DNA ; chemistry ; Genes ; Genes, erbB-2 ; Humans ; Pathology ; trends

Objectives: To assess DNA methylation state of the 13 genes in urine sediments and evaluate their value in diagnosis of bladder cancer. Methods: Ninety-two patients with bladder cancer, 23 patients with non tumorous urinary disease, 6 patients with neurological disease, and 7 healthy volunteers were recruited in the study. The DNA methylation state of tumor-related promoters in urine sediments was determined by Methylation specific PCR method (MSP). Results: The frequency of hypermethylation of 13 genes in 92 cases with bladder cancer was higher than that in 23 cases of bladder diseases, while all the genes were at demethylation state in the 7 healthy volunteers and 6 patients with neurological disease. The incidence of hypermethylation was significantly higher in bladder cancer compared with non tumorous urinary disease (P < 0.05). If DNA hypermethylation was used as a positive indicator, 88.0% (81/92cases) of bladder cancer could be detected. Conclusion: Assessing DNA methylation state in the promoter of tumor-related genes in urine sediments by MSP method is effective for detection of bladder cancer.

The 55th ASH annual meeting published a special report about Hodgkin lymphoma,which mainly contains management of early-stage Hodgkin lymphoma,novel targeted therapy and the optimal treatment of lymphocyte-predominant Hodgkin lymphoma.The therapy of Hodgkin lymphoma should be individualized,in order to improve both PFS and OS.More clinical trials should be designed on the target therapy and the treatment strategy of nodular lymphocyte-predominant Hodgkin lymphoma.

A 41 years female patient was hospitalized with hoarseness for three months, without cough, expectoration and dyspnea. The symptom had no relief by application of antibiotics. Electronic laryngoscope showed that there were the grey neoplasms under subglottic anteriou commissure with broad base and slightly rough surface, and bilateral vocal cords were normal; throat CT showed subglottic irregular high density and calcification. The subglottal tumor resection was performed under the general anesthesia and laryngoscope CO2 laser assisted. In the operation, we found that the tumor was hard with surface of thin mucosa tissue, and there was white bone tissue under it. The removing tumor was about 0. 8 cm X 0. 6 cm X 0. 3 cm and was diagnosed as subglottic osteoma by pathologic examine. There was no recurrence in three months follow-up.
Adult ; Female ; Glottis ; pathology ; Hoarseness ; complications ; Humans ; Lasers, Gas ; Neoplasm Recurrence, Local ; Osteoma ; diagnosis

Acupuncture Points ; Acupuncture Therapy ; Adolescent ; Adult ; Aged ; Female ; Humans ; Male ; Middle Aged ; Moxibustion ; Restless Legs Syndrome ; therapy ; Young Adult

Drug Combinations ; Drug Design ; Drugs, Chinese Herbal ; pharmacology ; Drugs, Investigational ; Humans

Anemia ; blood ; chemically induced ; drug therapy ; etiology ; therapy ; Antineoplastic Agents ; adverse effects ; Blood Transfusion ; Erythropoietin ; blood ; therapeutic use ; Hemoglobins ; metabolism ; Humans ; Neoplasms ; blood ; complications ; drug therapy ; Receptors, Erythropoietin ; blood

Objective:To explore the effect of infection on autophagy-related proteins,Beclin-1 and LC3,expression in cerebral white matter in newborn rats.Methods: A total of 64 two-day-old Sprague-Dawley(SD) rats were randomly divided into control and experimental groups(n=32 each).At day 2 to 6 after birth,the rats in experimental group were intraperitoneally injected with 0.6 mg/kg of lipopolysaccharide(LPS) once a day to establish a white matter injury induced by infection in neonatal rats while the rats in control group were injected with equal amounts of normal saline.Rats were sacrificed to collect brain tissues at 12 hour,1,3,5 d after model establishment.HE staining was performed to observe the pathological changes.Changes in the expression of Beclin-1 and LC3 in rat white matter were examined by Western blot and RT-PCR.Results: Growth and development of rat in experimental group was slow,cerebral white matter lesions were obvious.Compared with the control group,the experimental group Beclin1 and LC3 protein and mRNA levels in the model after 12 h began to express,1 d reached the peak,and then decreased,each time points were higher than the control group(P<0.05).Conclusion: Early infection in neonatal rats can cause white matter damage;the expression of autophagy-related proteins,Beclin1 and LC3,showed that autophagy may be involved in the pathological process of white matter damage induced by infection.

Objective To establish experimental autoimmune thyroiditis (EAT) rat model induced by bovine thyroglobulin (bTg) and to observe the effect of iodine on IP-10 in rat serum and IP-10 mRNA expression in rat thyroid tissue.Methods One hundred and thirty-five four-week old female Lewis rats were divided into normal control (NC,20 rats) group;TG group,25 rats;H Ⅰ group,20 rats;H Ⅰ + TG group,25 rats;H Ⅱ group,20 rats and H Ⅱ + TG group,25 rats according to a random number table.The water iodine concentration was 25.7 μg/L given to rats of HⅠ and H I + TG groups,and 423.3 μg/L of H Ⅱ and H Ⅱ + TG groups.Rats of NC and TG groups drank distilled water.Rats of TG,H Ⅰ + TG,H Ⅱ + TG groups were immunized with 0.1 ml bTg (8 g/L) in IFA.All rats were killed at the end of 15 weeks.Urinary iodine was determined by As3+-Ce4+ catalytic spectrophotometry (WS/T 107-2006).Pathological changes in thyroid tissue were observed by light microscope.Serum IP-10 was determined by enzyme-linked immunosorbent assay (ELISA),and IP-10 mRNA expression in thyroid was detected by real-time PCR.Results The differences of urinary iodine between groups were statistically significant (x2 =106.4,P ＜ 0.05).Compared with NC group (456.45 μg/L) urinary iodine in other groups increased significantly (TG,H Ⅰ,H Ⅰ + TG,HⅡ,HⅡ + TG:800.08,18 633.20,13 869.08,87 889.97,61 661.51 μg/L,all P ＜ 0.05).The pathological changes of rats in each group aggravated following increased iodine intake,NC group had normal thyroid structure;thyroid of TG group had a small amount of lymphocytes;thyroid of H Ⅰ group showed irregular follicular,part of the follicular was destroyed;a large number of lymphocytes infiltrated between follicular of H Ⅱ group;in H Ⅱ + TG group,the follicular was destroyed severely,diffuse inflammatory cell infiltration.The difference of serum IP-10 between groups no were statistically significant (F =1.462,P ＞ 0.05).The expression of IP-10 mRNA of H Ⅰ + TG (2.80 ± 1.73) rats thyroid was higher than that in NC (1.65 ± 1.62) and H Ⅰ (1.07 ± 1.00) groups,H Ⅱ (0.64 ± 0.64),H Ⅱ + TG (0.80 ± 0.49) were lower than H Ⅰ + TG group (all P ＜ 0.05).Conclusions Excessive iodine intake could have increased inflammatory cells in EAT rat and rats have showed more serious pathologic changes.These phenomena may be ralated with changed expression of IP-10 mRNA in EAT rat thyroid.