Adolescent ; Adult ; Child ; Child, Preschool ; Heart Defects, Congenital ; surgery ; Humans ; Young Adult

Adult ; Ascorbic Acid ; therapeutic use ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; administration & dosage ; therapeutic use ; Female ; Humans ; Infusions, Intravenous ; Male ; Myocarditis ; drug therapy ; virology ; Phytotherapy ; Virus Diseases ; drug therapy

Biomarkers ; analysis ; Environmental Monitoring ; methods ; Humans ; Occupational Exposure ; analysis ; Xylenes ; analysis

Adolescent ; Asthma ; blood ; drug therapy ; Case-Control Studies ; Child ; Child, Preschool ; Female ; Humans ; Interleukin-17 ; blood ; Male

Animals ; Autoantigens ; genetics ; metabolism ; physiology ; Centromere Protein A ; Chromosomal Instability ; Chromosomal Proteins, Non-Histone ; genetics ; metabolism ; physiology ; Gene Expression Regulation, Neoplastic ; Humans ; Kinetochores ; metabolism ; Mitosis ; physiology ; Rectal Neoplasms ; genetics ; metabolism ; pathology ; Spindle Apparatus ; metabolism

BACKGROUND:Previous studies demonstrated that ischemia can be compensated by establishing collateral circulation and microangiogenesis,and exercise training can ameliorate blood supply of ischemic lower limb.However,whether exercise training accelerate establishment of collateral circulation remains poorly understood.OBJECTIVE:To explore the mechanism of exercise training on accelerating microangiogenesis of rat ischemic lower limb.METHODS:Sprague-Dawley rats were randomly divided into the exercise training,model and sham-surgery groups.All animals were established left lower limb ischemia models except those in the sham-surgery group.Rats in the exercise training group were forced running 30 minutes per day at 1 week after model preparation and those in other groups were performed daily activities.The adductor of ischemic lower limb was obtained for the examination of microvessel density and the expressions of vascular endothelial growth factor and basic fibroblast growth factor.Simultaneously,bone marrow-derived endothelial progenitor cells were harvested for detection of microangiogenesis.RESULTS AND CONCLUSION:Number of endothelial progenitor calls,expressions of vascular endothelial growth factor and basic fibroblast growth factor,as well as microvessel density of the exercise training group was obviously greater than those in the model and sham-surgery groups(P<0.01).Compared with the model and sham-surgery groups,in vitro vasculogenesisof bone marrow-derived endothelial progenitor cells in the exercise training group was increased(P<0.05).These findings demonstrated that lower limb ischemia increases microangiogenesis,and exercise training enhances this effect.

This research was based on the theory and method of International Classification of Function, Disability and Health (ICF) and looked into the issues of physical function and disability. It tried to explore the purpose, knowledge structure and curriculum of special physical education that met the needs of social development and to restructure the curriculum and content of special physical education. Stu-dents of special physical education should not only master basic specialized knowledge, but also be able to practice theories, so as to better serve all kinds of children with special needs in training, assessment and intervention.

Acquired Immunodeficiency Syndrome ; diagnosis ; therapy ; Female ; Humans ; Male ; Syphilis ; diagnosis ; therapy

Objective To evaluate the long term efficacy of partial splenic embolization(PSE) for thalassanemia major. Methods 75 patients with thalassanemia major were treated with PSE and followed for 5 years,in which 30 patients with compelete data were studied.There were 8 patients with ? thalassanemia major,22 patients with ? thalassanemia major.The follow up indices included mean haemoglobin concertration,transfusion indices and blood transfusion consumption after embolization at 1,2,3,4,5 years. Results 26 of 30 patients showed a reduction in blood transfusion requirements and increase in haemoglobin level after embolization. The median blood transfusion requirements decreased from 1 200 ml/year before embolization to 200 ml/year; and the transfusion indices dropped from 6 times/year to 1 time/year. The mean of haemoglobin level increased from (60 9? 18 9)g/L to (82 3?17 4)g/L. The total effective rate was 86 7%,in which ? ,? thalassanemia major were 100 0%,81 8% respectively. Conclusion PSE is an effective therapeutic procedure for thalassanemia major, especially for ? thalassanemia major.

Objective: To investigate the expressions of hypoxia-inducible factor-1α (HIF-1α), glucose transporter 1 (GLUT1), multidrug resistance associated protein (MRP1), and glutathione-S-transferases (GST-π) in non-small cell lung cancer (NSCLC) tissues and their relationship with the biological features of lung cancer. Methods: The expressions of HIF-1α, GLUT1, MRP1, and GST-π mRNA in 36 samples of NSCLC tissues and 12 samples of corresponding adjacent tissues were detected by RT-PCR and semiquantitative agarose gel electrophoresis. Their expression in various TNM staging cancer tissues was analyzed by SAS8.0 statistic software. Results: The expressions of the four genes significantly increased in NSCLC tissues than in the corresponding adjacent tissues (P < 0.01). The expressions of the four genes were significantly higher in the group with lymph node and distant metastasis than that in the groups without lymph node and distant metastasis (P < 0.01 or P < 0.05). The expressions of HIF-1α and GLUT1 mRNA were significantly increased in T3 and T4 staging cancer tissues than that in T1 and T2 staging cancer tissues (P < 0.01 or P < 0.05). The expressions of MRP1 and GST-π mRNA had no significant difference between NSCLC tissues at T1, T2, T3 and T4 staging(P >0.05). Conclusion: HIF-1α, GLUT1, MRP1, and GST-π were over-expressed in NSCLC tissues, which were associated with lymph node and distant metastasis. Expressions of HIF-1α and GLUT1 were elevated with the increase in the TNM staging but expressions of MRP1 and GST-π did not correlate with TNM staging. HIF-1α mRNA expression was positively related with the expression of GLUT1 mRNA (P < 0.01). The expression of HIF-1α mRNA was significantly related with the expression of MRP1 and GST-π mRNA in linear dependency (P <0.05). The expression of GLUT1 mRNA was not significantly related with the expression of MRP1 and GST-π mRNA (P > 0.05). The over-expressions of the four genes may be useful markers indicating the carcinogenesis, Progression, and deterioration of NSCLC.