2.Clinical analysis of a child with cardio-facio-cutaneous syndrome due to a de novo variant of MAP2K1 gene.
Hongyao CAO ; Guanglei TONG ; Ru HUANG ; Taocheng ZHOU ; Weiwei ZHANG
Chinese Journal of Medical Genetics 2022;39(10):1129-1134
OBJECTIVE:
To explore the genotype-phenotype correlation of a patient with cardio-facio-cutaneous syndrome (CFCS) due to variant of the MAP2K1 gene.
METHODS:
DNA was extracted from peripheral blood samples of the infant and his parents and subjected to whole exome sequencing. Candidate variant was verified by Sanger sequencing.
RESULTS:
The patient had typical CFCS facies and developmental delay, and was found to harbor a de novo heterozygous c.389A>G (p.Tyr130Cys) missense variant in exon 3 of the MAP2K1 gene. Based on the American college of Medical Genetics and Genomics guidelines, this variant was classified as likely pathogenic.
CONCLUSION
This patient has differed from previously reported cases by having no cardiac anomaly or seizures but typical facial features and skin abnormalities accompanied by growth retardation, intellectual impairment, and urinary malformation. It has therefore enriched the phenotypic spectrum of CFCS due to variants of the MAP2K1 gene.
Ectodermal Dysplasia/genetics*
;
Facies
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Failure to Thrive/genetics*
;
Heart Defects, Congenital
;
Humans
;
MAP Kinase Kinase 1/genetics*
;
Mutation
3.Molecular mechanism of FGF8b regulation of epithelial-mesenchymal transition in prostate cancer cells.
Benyi FAN ; Guilin WANG ; Fan QI ; Zhuo LI ; Huaizheng LIU
Journal of Central South University(Medical Sciences) 2012;37(7):656-661
OBJECTIVE:
To explore the molecular mechanism of fibroblast growth factor 8b (FGF8b) in promoting epithelial-mesenchymal transition in prostate cancer DU145 cells.
METHODS:
Cells were selected in three groups as follows: a block control group (DU145 cells), a negative control group [DU145 cells transfected with empty plasmid (pcDNA3.1/DU145)], and an experimental group [DU145 cells transfected with FGF8b (FGF8b/DU145)]. The activity of extracellular regulated protein kinases1/2( ERK1/2) pathway was detected by western-blot in the three groups. The FGF8b-DU145 cells and DU145 cells were cultured with PD98059 (an ERK kinase inhibitor) to observe microscopically the morphology changes within the cells. The experimental samples were also divided into four groups: FGF8b/DU145 cells cultured with 2% FBS (Group A); FGF8b/DU145 cells cultured with 2% FBS+PD98059 (50 μmol/L) (Group B); DU145 cells cultured with 2% FBS (Group C); DU145 cells cultured with FBS+PD98059 (50 μmol/L) (Group D). The expression of epithelial- mesenchymal transition (EMT) markers (E-cadherin, vimentin) were detected by western-blot analysis and the cell's mobility were detected by the Transwell chamber.
RESULTS:
The activity of ERK1/2 in the experimental group was significantly higher than that in the other two control groups; when ERK kinase inhibitor PD98059 was added to FGF8b/ DU145 cells, the expression of epithelial marker E-cadherin protein was significantly increased in group B compared with that in the group A (P<0.05). The expression of mesenchymal marker vimentin protein was significantly reduced in group B compared with that in group A (P<0.05). The cell migration assay suggested that cell migration was markedly decreased in group B (P<0.05) compared with that in group A.
CONCLUSION
EMT in prostate cancer induced by FGF8b can be mediated by ERK kinase pathway, in which mitogen-activated/extraceluer signal regulated kinase 1 (MEK1) may be a key factor. MEK1 could be an effective target in regulating the invasion and migration of prostate cancer.
Epithelial-Mesenchymal Transition
;
genetics
;
Fibroblast Growth Factor 8
;
genetics
;
metabolism
;
Flavonoids
;
pharmacology
;
Humans
;
MAP Kinase Kinase 1
;
metabolism
;
MAP Kinase Signaling System
;
physiology
;
Male
;
Neoplasm Invasiveness
;
Neoplasm Metastasis
;
Prostatic Neoplasms
;
genetics
;
metabolism
;
pathology
;
Transfection
;
Tumor Cells, Cultured
4.A Case of Pulmonary Langerhans Cell Histiocytosis with BRAF V600E-negative and MAP2K1-posivtive Mutations Presenting as Diffuse Nodules in Chest High-resolution Computed Tomography After Smoking Cessation.
Yan LI ; Hou-Rong CAI ; Miao MA ; Li-Jing WANG ; Jun YANG ; Jing-Jing DING ; Li-Yun MIAO
Chinese Medical Journal 2016;129(11):1384-1386
5.MEK1 and MEK2 differentially regulate human insulin- and insulin glargine-induced human bladder cancer T24 cell proliferation.
Shan-Ying LIU ; Ying LIANG ; Tian-Xin LIN ; Fang SU ; Wei-Wen LIANG ; Heemann UWE ; Yan LI
Chinese Medical Journal 2012;125(23):4197-4201
BACKGROUNDIncreased risk of bladder cancer has been reported in diabetic patients. This study was to investigate the roles of mitogen-activated protein kinase kinase (MEK) 1 and 2 in the regulation of human insulin- and insulin glargine-induced proliferation of human bladder cancer T24 cells.
METHODSIn the absence or presence of a selective inhibitor for MEK1 (PD98059) or a specific siRNA for MEK2 (siMEK2), with or without addition of insulin or glargine, T24 cell proliferation was evaluated by cell counting kit (CCK)-8 assay. Protein expression of MEK2, phosphorylation of ERK1/2 and Akt was analyzed by Western blotting.
RESULTST24 cell proliferation was promoted by PD98059 at 5 - 20 µmol/L, inhibited by siMEK2 at 25 - 100 nmol/L. PD98059 and siMEK2 remarkably reduced phosphorylated ERK1/2. Insulin- and glargine-induced T24 cell proliferation was enhanced by PD98059, suppressed while not blocked by siMEK2. Insulin- and glargine-induced ERK1/2 activation was blocked by PD98059 or siMEK2 treatment, whereas activation of Akt was not affected.
CONCLUSIONMEK1 inhibits while MEK2 contributes to normal and human insulin- and insulin glargine-induced human bladder cancer T24 cell proliferation.
Blotting, Western ; Cell Line, Tumor ; Cell Proliferation ; drug effects ; Flavonoids ; pharmacology ; Humans ; Insulin ; pharmacology ; Insulin Glargine ; Insulin, Long-Acting ; pharmacology ; MAP Kinase Kinase 1 ; antagonists & inhibitors ; metabolism ; MAP Kinase Kinase 2 ; genetics ; metabolism ; MAP Kinase Signaling System ; drug effects ; genetics ; Phosphorylation ; drug effects ; RNA, Small Interfering ; genetics ; physiology ; Urinary Bladder Neoplasms ; metabolism
6.The mRNA expression of mitogen-activated protein kinase signal pathway related genes in the blood of arseniasis patients caused by burning coal.
Peng LUO ; Ai-hua ZHANG ; Yun XIAO ; Xue-li PAN ; Xue-xin DONG ; Xiao-xin HUANG
Chinese Journal of Preventive Medicine 2013;47(9):788-793
OBJECTIVETo detect the mRNA expression of ERK1, ERK2, JNK1 and P38 gene in mitogen-activated protein kinase(MAPK) path way in the arseniasis patients caused by burning coal.
METHODS70 arseniasis patients caused by burning coal at Jiaole village XingRen county in December 2006 were selected as case group, and another 30 villagers with similar living habits, matched gender and age, healthy physical condition without history of burning high arsenic coal were selected as control group from 12 km nearby the same village.Silver diethyl dithiocarbamate method (Ag-DDC) was taken to detect the arsenic contents in the environmental media, food, and arsenic level in the urine and hair of arseniasis patients.On the principle of informed consent, the peripheral blood was collected from the patients. The total RNA was extracted with Trizol method and cDNA was reversed from it. The mRNA expression of ERK1, ERK2, JNK1 and P38 gene in MAPK path way were tested by real-time fluorescent quantitative PCR (QT-PCR).
RESULTSA total of 70 cases of arseniasis patients (31 cases of mild, 25 cases of moderate and 14 cases of severe) and 30 cases of control were chosen. The median (quartile) of arsenic contents in the indoor air, outdoor air, coal, chili and corn were 0.079 (0.053-0.117) mg/m(3) ,0.007 (0.002-0.015) mg/m(3) , 93.010 (39.460-211.740) mg/kg, 3.460(0.550-16.760) mg/kg and 1.500(0.300-4.140) mg/kg respectively. They were above the national health standards. The median (quartile) of arsenic contents in the soil, rice and drinking water were separately 12.130(4.230-24.820) mg/kg, 0.650(0.300-0.980) mg/kg and 0.043(0.012-0.089)mg/kg, which were within the national health standards. Compared with the control group ((26.97 ± 9.71)µg/g Cr), arsenic level in the patients' urine ((71.48 ± 22.74)µg/g Cr) increased significantly, the differences were significant (F = 90.38, P < 0.01). Compared with the control group ((1.58 ± 1.07)µg/g), arsenic level in the patients' hair ((4.45 ± 2.78) µg/g) increased significantly, the differences were significant (F = 48.22, P < 0.01). The relative expression amount of the median(quartile) for ERK2, JNK1 mRNA were 0.0667 (0.0378-0.1371) and 0.0013 (0.0009-0.0025), respectively. Compared with the control group 0.1744 (0.1009-0.1985) and 0.0022 (0.0017-0.0030) , only the decreases of ERK2, JNK1 mRNA expression was significant (χ(2) = 15.10, 14.25, P < 0.01), and no significance in the other index. ERK2 mRNA relative expression for mild, medium and severe groups were separately 0.0818 (0.0408-0.1509) ,0.0582 (0.0154-0.1699) and 0.0588 (0.0399-0.1034) . Compared with the control group (0.1744 (0.1099-0.1985) ), there was significant difference (Z = -2.89, -3.19, -2.67, P < 0.01). JNK1 mRNA relative expression were 0.0012 (0.0007-0.001 57), 0.0019 (0.0011-0.0035), 0.0013 (0.0010-0.0026), respectively. Compared with the control group (0.0022 (0.0017-0.0030) ), significances were found in the mild groups (Z = -3.72, P < 0.01).
CONCLUSIONSArsenic could induce the changes of ERK2 and JNK1mRNA expression in the MAPK path way in arseniasis patients.It suggests that the MAPK signaling pathway take part in the occurrence and development process of arseniasis caused by burning coal.
Adult ; Air Pollution, Indoor ; Arsenic Poisoning ; blood ; etiology ; Case-Control Studies ; Coal ; Female ; Humans ; MAP Kinase Signaling System ; Male ; Middle Aged ; Mitogen-Activated Protein Kinase 1 ; blood ; genetics ; Mitogen-Activated Protein Kinase 8 ; blood ; genetics ; RNA, Messenger ; genetics ; Transcription, Genetic
7.Calcitonin induces connective tissue growth factor through ERK1/2 signaling in renal tubular cells.
Misa NAKAMURA ; Takashi OZAKI ; Aiko ISHII ; Masayoshi KONISHI ; Yuji TSUBOTA ; Toru FURUI ; Hayato TSUDA ; Ichiro MORI ; Kiichiro OTA ; Kennichi KAKUDO
Experimental & Molecular Medicine 2009;41(5):307-314
Calcitonin (CT), a polypeptide hormone, plays important roles in a variety of physiological processes. CT has been used clinically to treat osteoporosis and humoral hypercalcemia of malignancy. In order to clarify the pharmacological effects of CT in the kidney, we identified potential downstream genes induced by CT in the renal cells. Using a cDNA subtraction hybridization method, we identified connective tissue growth factor (CTGF) as a CT-induced gene in the porcine renal cell line, LLC-PK1. Furthermore, we found that CT-mediated induction of the gene was not inhibited by cycloheximide, which suggests that CTGF gene was not induced by an increased synthesis of regulating proteins. Therefore, CTGF is an immediate early gene. We further demonstrated that the regulation of CTGF gene expression by CT involved the ERK1/2 pathway, because PD98059, a MEK1 inhibitor, partially inhibited the mRNA expression of CTGF induced by CT. CT-induced CTGF protein expression was also observed in vivo. Our present findings suggest that CT induces the transcription of CTGF through ERK1/2 phosphorylation. We also identified twelve other genes induced by CT that, like CTGF, were related to wound healing. These results suggest that CT may have an effect on renal differentiation and wound healing in the kidney.
Animals
;
Calcitonin/*pharmacology
;
Cell Line
;
Connective Tissue Growth Factor/*genetics/metabolism
;
Female
;
Kidney Tubules, Proximal/*enzymology/metabolism
;
*MAP Kinase Signaling System
;
Mice
;
Mice, Inbred BALB C
;
Mitogen-Activated Protein Kinase 1/*metabolism
;
Mitogen-Activated Protein Kinase 3/*metabolism
;
Phosphorylation
;
Swine
8.Calcitonin induces connective tissue growth factor through ERK1/2 signaling in renal tubular cells.
Misa NAKAMURA ; Takashi OZAKI ; Aiko ISHII ; Masayoshi KONISHI ; Yuji TSUBOTA ; Toru FURUI ; Hayato TSUDA ; Ichiro MORI ; Kiichiro OTA ; Kennichi KAKUDO
Experimental & Molecular Medicine 2009;41(5):307-314
Calcitonin (CT), a polypeptide hormone, plays important roles in a variety of physiological processes. CT has been used clinically to treat osteoporosis and humoral hypercalcemia of malignancy. In order to clarify the pharmacological effects of CT in the kidney, we identified potential downstream genes induced by CT in the renal cells. Using a cDNA subtraction hybridization method, we identified connective tissue growth factor (CTGF) as a CT-induced gene in the porcine renal cell line, LLC-PK1. Furthermore, we found that CT-mediated induction of the gene was not inhibited by cycloheximide, which suggests that CTGF gene was not induced by an increased synthesis of regulating proteins. Therefore, CTGF is an immediate early gene. We further demonstrated that the regulation of CTGF gene expression by CT involved the ERK1/2 pathway, because PD98059, a MEK1 inhibitor, partially inhibited the mRNA expression of CTGF induced by CT. CT-induced CTGF protein expression was also observed in vivo. Our present findings suggest that CT induces the transcription of CTGF through ERK1/2 phosphorylation. We also identified twelve other genes induced by CT that, like CTGF, were related to wound healing. These results suggest that CT may have an effect on renal differentiation and wound healing in the kidney.
Animals
;
Calcitonin/*pharmacology
;
Cell Line
;
Connective Tissue Growth Factor/*genetics/metabolism
;
Female
;
Kidney Tubules, Proximal/*enzymology/metabolism
;
*MAP Kinase Signaling System
;
Mice
;
Mice, Inbred BALB C
;
Mitogen-Activated Protein Kinase 1/*metabolism
;
Mitogen-Activated Protein Kinase 3/*metabolism
;
Phosphorylation
;
Swine
9.Extracellular signal regulated kinase 5 promotes cell migration, invasion and lung metastasis in a FAK-dependent manner.
Weiwei JIANG ; Fangfang CAI ; Huangru XU ; Yanyan LU ; Jia CHEN ; Jia LIU ; Nini CAO ; Xiangyu ZHANG ; Xiao CHEN ; Qilai HUANG ; Hongqin ZHUANG ; Zi-Chun HUA
Protein & Cell 2020;11(11):825-845
This study was designed to evaluate ERK5 expression in lung cancer and malignant melanoma progression and to ascertain the involvement of ERK5 signaling in lung cancer and melanoma. We show that ERK5 expression is abundant in human lung cancer samples, and elevated ERK5 expression in lung cancer was linked to the acquisition of increased metastatic and invasive potential. Importantly, we observed a significant correlation between ERK5 activity and FAK expression and its phosphorylation at the Ser
A549 Cells
;
Animals
;
Cell Movement
;
Epithelial-Mesenchymal Transition/genetics*
;
Focal Adhesion Kinase 1/metabolism*
;
Humans
;
Lung Neoplasms/pathology*
;
MAP Kinase Signaling System
;
Mice
;
Mitogen-Activated Protein Kinase 7/metabolism*
;
Neoplasm Invasiveness
;
Neoplasm Metastasis
;
Neoplasm Proteins/metabolism*
10.MAPK signal pathway plays a role in proliferation of Jurkat cells induced by ouabain.
Run-Ming JIN ; Yan BAI ; An-Xiu XIONG ; Wen LIN ; Hui YU ; Xiao-Yan WU ; Hong-Bao FEI
Journal of Experimental Hematology 2005;13(1):126-129
The object was to study the effect of ouabain on Jurkat cells and its possible mechanism. The effect of ouabain of low concentration on Jurkat cells was confirmed by MTT, while c-myc gene transcription was measured by RT-PCR, and the phosphorylation of MAPK (ERK1/2) as well as the expression of c-myc gene was tested by Western blot respectively. The results showed that ouabain at low concentration could induce the proliferation of Jurkat in a time-and dose-dependent manner. At the same time, the phosphorylation of MAPK (ERK1/2) and the expression of c-myc gene was enhanced. In conclusion, ouabain stimulates the intracellular MAPK signal pathway by acting on the Na, K-ATPase, and thus induce the proliferation of Jurkat cells, in which the regulation of c-myc gene expression may be involved.
Blotting, Western
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Cell Proliferation
;
drug effects
;
Dose-Response Relationship, Drug
;
Extracellular Signal-Regulated MAP Kinases
;
metabolism
;
Humans
;
Jurkat Cells
;
MAP Kinase Kinase 1
;
metabolism
;
MAP Kinase Signaling System
;
physiology
;
Ouabain
;
pharmacology
;
Phosphorylation
;
drug effects
;
Proto-Oncogene Proteins c-myc
;
genetics
;
metabolism
;
RNA, Messenger
;
genetics
;
metabolism
;
Reverse Transcriptase Polymerase Chain Reaction
;
Time Factors