Objective To explore the clinical efficacy of percutaneous vertebroplasty technique(PVP) in the patients with senile osteoporotic thoracolumbar compression fractures (OVCFs) and to explore the main factors of PVP.Methods PVP treatment of OVCFs 46 cases,in the perspective of C-arm X-ray,bone cement as a filling material,transpedicular puncture and injection of 59 vertebrae.Results The pain in 35 cases(76.09％) OVCFs patients apparent ease or disappear,the remaining 11 cases of moderate relief;follow-up period(3 to 15)months(mean 8.6months).After X-ray review,the patient' s bone cement dispersion was good and has improved vertebral height has not been lost,activity and quality of life significantly inproved.Conclusion PVP is simple,less invasive,etc.,which can quickly reduce pain and improve their quality of life.

To explore the characteristics of benign paroxysmal positional vertigo (BPPV) and examine the efficacy of Canalith repositioning in elders.A retrospective study was carried out among 47 BPPV cases at our hospital from January 2011 to June 2012.Posterior semicircular canal received Epley maneuver (n =35) and Lempert rolling for horizontal semicircular canal (n =12).Curative effect was evaluated after 1 week and 3 months.After Canalith repositioning,the initial success rate was 91.5％ (43/47) and the effective rate 8.5％ (4/47).After 1 week,the success rate was 100％.Nine patients (19.1％) had recurrence in the same canal previously affected,and all improved with a new repositioning maneuver.Canalith repositioning is both safe and effective in the treatment of BPPV.

Radiotherapy is an important method of tumor treatment .The incidence of complications caused by radiotherapy is high, which seriously effect patients′quality of life.The current terms of radiation enteritis , radiation proctitis and radiation cystitis can not reflect the changes of physiopathology after radiotherapy .Nowadays , a consensus has been gradually reached that 'pelvic radiation disease'is a more accurate description .This article mainly introduces the definition evolution , significance and current status of pelvic radiation disease to draw the attention of domestic related professionals .A new starting point is opening for the curing of radiotherapy-related complications .

Objective: To study the phenotype characteristic of mandibular condylar chondrocytes (MCCs) in vitro. Methods: MCCs of two-week-old New Zealand rabbits were isolated with enzyme digestion and cultured in DMEM. Phase contrast microscopy, image analysis system, MTT assay and immunocytochemistry method were used to compare MCCs of different passages in cellular shape and size, growth curve and expression of type I and type II collagen. Results: The majority of MCCs in earlier passages (1～3 passage) were polygonal, while more fusiform and spindle-shaped cells were found after 5～6 passages. MCCs in earlier passages were smaller than those in sixth passages. The proliferation rate of MCCs decreased with passaging. There were more type II collagen positive cells in 1 ～ 3 passages, while more type I collagen positive cells in 7th passage. Conclusion: The changing characters of MCCs in vitro such as cellular shape and size and expression of type I and II collagen are similar to those of MCCs in vivo from proliferating zone to hypertrophic zone. MCCs in later passages may be with dedifferentiation.

Objective To evaluate the value of laparoscopic-assisted cholangiography in diagnosing prolonged jaundice in infants.Methods Through an umbilical troear,an laparoscope was placed into the abdominal cavity to detect the gallbladder and liver.After confirming that the choleeyst is normal,we pulled out the fundus of the gallbladder through the right subcostal trocar,and then inserted a catheter into the gallbladder for cholangiography.If the fundus could not be exteriorized because of gallbladder atresia, the patient would be converted to an open surgery.Results Cholangiography showed infant hepatitis syndrome or cholestasis in 8 cases,biliary hypoplasia in 2,and biliary atresia in 2.In 5 patients,the gallbladder was dissected from the liver bed before cholangiography,2 of them had biliary hypoplasia and 3 showed biliary atresia.Cholangiography was given up in 21 children because of liver cirrhosis.These patients were diagnosed with biliary atresia and then were treated by Kasai portoenterostomy. Conclusions Laparoscopic-assisted cholangiography is a simple,accurate,and safe method in the diagnosis of prolonged jaundice in infants.By using the procedure,the whole biliary tree can be shown clearly without leading to serious injuries.

Objective To study the method of Traditional Chinese Medicine EMR System application evaluation, design the evaluation index. so that to make the Traditional Chinese medicine EMR system evaluation more scientific and normative. Methods Based on the current situation of traditional Chinese medicine EMR system functional evaluation and the evaluation methods of EMR System Function Application Level Classification Evaluation Methods and Standards (Trial)formerly issued by the Ministry of Health to research traditional Chinese medicine EMR system application evaluation methods. Results Evaluation methods of traditional Chinese medicine EMR system should be the establishment form 3 part:establishing evaluation index system and evaluation standard, establishing qualitative and quantitative evaluation, and establishing evaluation scores system. Conclusion Traditional Chinese medicine EMR system application evaluation method provides a scientific basis for designing traditional Chinese medicine EMR system evaluation index system.

Aim ToinvestigatewhethertheJAK2/STAT3 signaling pathway regulates prohibitin expres-sion to protect cardiomyocytes against hypoxia/reoxy-genation injury in hydrogen sulfide postconditioning. Methods Primaryculturedcardiomyocytesfromneo-natal rats were divided into 6 groups: control group ( Normal) , hypoxia/reoxygenation group ( H/R ) , hy-drogen sulfide postconditioning group ( NP) , hydrogen sulfide with AG490 group ( N + A ) , AG490 group ( AG) , DMSO group ( DMSO) . The survival percent-age of cardiomyocytes and the release of LDH were tested at pre-hypoxia and reoxygenation 2h. After reox-ygenation, cell apoptosis was detected by flow cytome-try. The expression of t-STAT3, p-SATAT3 and PHB were determined with Western blot analysis. Results No obvious changes were observed among the groups before hypoxia (P <0. 05). After reoxygenation 2h, compared with H/R group, NP group significantly im-proved the survival rate of cardiomyocytes ( P <0. 05 ) , inhibited the release of LDH and the myocardi-al apoptosis ( P <0. 05 ) , meanwhile up-regulated the p-STAT3 and PHB expression. However, AG490 abol-ished the cardioprotection offered by hydrogen sulfide postconditioning and the increase in p-STAT3 and PHB expression.Conclusion Hydrogensulfidepostcondi-tioning may protect cardiomyocytes against hypoxia/reoxygenation injury through the JAK2/STAT3 pathway upregulating the expression of prohibitin.

Objective To study the clinical efficacy of full thoracoscopic lobectomy and thoracotomy in treat-ment of non -small cell lung cancer.Methods 200 patients with non -small cell lung cancer were divided into group A and group B according to the different surgical methods,with 100 cases in each group.Group A was given the full treatment thoracoscopic lobectomy,group B was received treatment thoracotomy.Pain situation were evaluated in visual analog scale(VAS)method,clinical efficacy and adverse reactions of the two groups were compared.Results The intraoperative blood loss,operative time,drainage,VAS scores and hospital stay of group A were (253.4 ± 1.9)mL,(181.3 ±9.3)min,(174.6 ±4.2)mL,(4.2 ±1.4)points and (7.2 ±0.6)d respectively,which were significantly better than (409.3 ±2.4)mL,(232.2 ±3.2)min,(312.9 ±2.5)mL,(6.3 ±0.9)points and (13.5 ± 0.6)d of group B,the differences were statistically significant(t =8.928,9.028,8.232,9.701,10.906,all P <0.05).The lymph node metastasis number and the number of lymph node dissection of the two groups had no statistically significant differences(t =2.098,3.092,2.983,all P >0.05).The incidence of complications of group A were significantly lower than those of group B,the difference was statistically significant(χ2 =9.033,P =0.002). Conclusion Full thoracoscopic lobectomy in the treatment of non -small cell lung cancer has better effect compared with thoracotomy,its harm to patients is small,and has fewer postoperative complications.

Objective To assess the middle-term results of laparoscopy in resection of congenital choledochal cyst,hepaticojejunostomy,and extra-abdominal Roux-en-Y anastomosis. Methods We retrospectively studied 50 patients who had undergone laparoscopic choledochal cyst excision in our institute from 2005 to 2008.For large cysts,we dissected and separated the cyst at the middle portion,and then opened the inferior wall of the cyst to dissect the posterior wall of the cyst from the portal vein.For small cysts,we divided the cysts circumferentially and then completely excised the dilated bile duct.When the cyst was intensely inflamed,excision of the cysts was performed as Lilly's technique.Roux-en-Y jejunojejunostomy was performed extracorporeally through an umbilical incision(1.5 cm),afterwards,end-to-side anastomosis was carried out intracorporeally.The conversion to open surgery,and morbidity and mortality rates of the patients was analyzed.Results The cysts were completely excised in 16 cases,and Lilly's technique was performed on 34 cases.One patient was converted to open surgery due to hemorrhage resulted from recurrent inflammatory-caused tight adhesion between the cyst and surrounding tissues.The mean operation time was 226 minutes(range: 190 to 450 minutes).Eight patients received blood transfusion during the operation.The children were discharged from hospital in 6 to 16 days after the surgery(mean,8 days).A mean of 26 months follow-up was achieved in 49 patients(ranged 3 to 39 months).Four patients developed complications: one of them showed bile leakage and then were cured by intra-abdominal drainage;one patient developed acute pancreatitis and one suffered from adhesive small bowel obstruction,both recovered after conservative treatment;intestinal necrosis was detected in one patient,who received another operation for resection of the necrotic bowel and anastomosis of the bile duct and intestine.In the other 45 patients,no abdominal pain,fever,jaundice,etc.were detected.No patient showed bile duct stenosis or reflux gastritis,no one died because of the surgery.Conclusions Laparoscopic approach is effective for congenital choledochal cyst,hepaticojejunostomy,and extra-abdominal Roux-en-Y anastomosis.Dissection of the posterior cyst wall to avoid injury to the portal vein is the key to the procedures,while extra-abdominal Roux-en-Y anastomosis is one of the most difficult steps.

Objective To assess the efficacy and safety of the Nuss procedure in the treatment of pectus excavatum in children. Methods The Nuss procedure was performed in 38 patients.Under thoracoscopy,a convex steel bar was inserted under the sternum through small bilateral thoracic incisions,with the convexity facing posteriorly.When the bar was in position,it was turned over,thereby correcting the deformity.Two lateral stabilizing bars were employed.Out of the 38 patients,imported bars were used in 5 patients and China-made bars were used in 33 patients.Results The procedure was completed under thoracoscopy smoothly in all the 38 patients.The operating time was 40~80 min(mean,50 min) and the intraoperative blood loss was 5~30 ml(mean,16 ml).A single bar was utilized in 36 patients,and a second bar was required in 2.Complications included postoperative pneumothorax in 4 patients,subcutaneous emphysema in 16 patients,bar displacement in 1 patient,and lateral stabilizer dislocation in 1 patient.The length of postoperative hospital stay was 7~21 days(mean,8 days).Follow-up checkups in the 38 patients for 3~22 months(mean,11 months) showed excellent outcomes in 36 cases and good outcomes in 2 patients,the rate of excellent or good results being 100%. Conclusions The Nuss procedure is safe and reliable for the treatment of pectus excavatum in children.The best age range for surgery was 3~12 years old.