Objective:To investigate the value of YL-1 puncture hematoma evacuation for hypertensive intracerebral hematoma.Methods:138 patients with hypertensive intracerecbral hematoma treated by YL-1 puncture hematoma evacuative were studied retrospectively,and the relationship between the age,the time of operation,GCS and volume of hemorrhage with mortality were with logistic regression method analysis.Results:①There were no significant relation in all the ages;②the shorter the hemorrhage,the higher the mortality;③the lower GCS was,the higher the mortality was;④the bigger the hemorrhage volume,the higher the mortality.Conclusion:The present study indicates that age is not an factor to limit minimally invasive operation(YL-1 puncture hematoma evacuation).The effect of surgery depended on the time of hemorrhage,GCS and volume of hemorrhage.

Objective:To evaluate and analyze the application value of Acute Physiology and Chronic Health EvaluationⅢ(A- PACHEⅢ)in evaluation of the severity and prognosis of patients in SCIU.Methods:By collecting and sorting out the data of 341 cases of surgical serious patients,the severity and prognosis of patients were evaluated by APACHEⅢ.Results:Making comparison among the different types of diseases,the scores and mortality of cerebral hemorrhage and multiple hurt were high- er than those of the diseases(P

Objective: To study the marriage quality of different occupational groups. Methods: 200 married teachers from two universities and 144 married employees in hospitality industry in Hainan Province were applied Olson 's Questionnaire on Marriage Quality. Results: First, the total score of the teachers was higher than that of the employees (35. 1?6. 7/33. 5 ?6. 6, t =5. 59, P =0. 00) . Marriage satisfaction, character compatibility, communications between husbands and wives, while the arrangements of their incomes and leisure activities were also higher in teachers (t = 2. 20 -3. 37, P

Objective To study clinical characteristics of the thalamic hemorrhage,probe into the correlation factor of judged prognosis in early days and case-fatality rate.Methods To 2003—2005 96 thalamic hemorrhage sufferer's clin- ical data were analysed.Results The advanced age and high blood pressure is main cause of disease of the thalamic hemorrhage.Conclusion To observe the thalamic hemorrhage sufferer's vital signs have the certain reference value to judged prognosis,case-fatality rate and serious complications were related to bloods closely in early days,whether break into the brain room,hypothalamus and the brain stem whether implicate.

Objective To understand the clinical medicine graduates competency status of Kunming Medical University in the past 5 years and provide important reference for cultivating students in medical university. Methods The questionnaire results in the relevant departments responsible person of Yunnan provincial municipal and district 26 hospitals were system analyzed by fuzzy comprehensive evaluation method. Results In the past 5 years, the graduates occupation competence of clinical medicine in Kunming Medical University is good. Among them, the graduates’core competency gets the lowest score, but the graduates’post competency gets the highest score. Conclusion The graduates’core competency should be strengthened in training of doctor-patient communication skills and quality evaluation of clinical practice for medical students.

AIM:So far,endostatin is found playing the strongest effect to inhibit endothelial cells.Because endostatin protein is instable,it is difficult to prepare and apply.Gene therapy is used to assist endostatin to inhibit angiogenesis.This study constructed recombinant adenovirus of human endostatin gene using AdEasy-1 system to investigate the feasibility of expressing endostatin protein in ECV304 cells(human umbilical vein endothelial cells).METHODS:The experiment was performed at College of Life Science,Sun Yat-set University from March to October 2006.Endostatin was amplified from Pshuttle-Endostatin plasmid with PCR and subcloned into the pAdTrack-CMV shuttle vector after sequencing and identification.The resultant plasmid was co-transduced into E.coli BJ 5183 cells with pAdEasy-1 plasmid for homologous recombination.The recombinant plasmid was detected by restriction analysis and DNA sequence analysis.The linearized recombinant plasmid was transfected into AAV 293 cells with LipofectAMINE2000.The recombinant adenovirus was detected by GFP,PCR and restriction analysis.The virus was used to infect ECV304,and the expression of endostatin in vitro was detected by the techniques of RT-PCR and Western blot.RESULTS:The positive clones of the recombinants were constructed and confirmed by restriction endonuclease analysis and DNA sequence analysis.AAV293 cells were transfected.The titer of viral liquid was 2.06?1010 pfu/mL.The transcription of endostatin mRNA in ECV304 cells was checked by RT-PCR and endostatin protein could be detected in the culture by Western blot analysis.CONCLUSION:The recombinant adenoviral vector pAd-Endo carrying human endostatin is successfully constructed,and effective expression is found in ECV304 cells in vitro.

Objective To investigate the clinical efficacy of ICARE rebounder tonometer (RBT) by comparing the measurement results of ICARE RBT,applanation tonometer (AT) and non-contact tonometer (NCT).Methods This study included 100 glaucoma suspect cases (100 eyes) with their day and night intraocular pressure (IOP) measurement in Beijing Tongren Hospital from May to November,2014.IOP of each eye was measured by two experienced examiners using RBT,AT and NCT for three times respectively and the mean values were recorded as the final result.Results IOP was measured by RBT,AT,NCT,the values were (19.1s5.7),(18.9±5.0),(15.2±5.0) mmHg (1 mmHg=0.133 kPa),respectively.The difference between RBT and AT was (0.2±1.8) mmHg,and the difference between NCT and AT was-(3.8±2.6) mmHg.There was no significant difference in the values between RBT and AT,t=-1.136,P＞0.05.There was significant difference in the values between NCT and AT,t=14.714,P＜0.01.The results measured by RBT,NCT and AT had correlation,r=0.948,0.822;P＜0.01.The relevant value was 0.973 in two examiners with RBT,which consisted with the result with AT.Conclusion RBT meets the clinical needs and can be widely used in clinical work.

Objective To investigate the correlation and risk factors between vaginal intraepithelial neoplasia (VAIN) and cervical intraepithelial neoplasia (CIN).Methods A total of 121 cases of VAIN patients was retrospectively analyzed with examination results such as clinical manifestations,liquid based cytology (TCT),human papilloma virus infection,virus infection type,and clinical data of treatment and follow up.Results VAIN Ⅰ,VAIN],and VAIN Ⅲ] were accounted for 33.9％ (41/121),38.0％ (46/121),and 24.4％ (34/121) ; for 40 or less years old patients,VAIN Ⅲ was accounted for 10.7％ (3/28) ; for more than 40 years old patients,VAIN Ⅲ was accounted for 33.3％ (31 /93).Among 121 VAIN patients,77.7％ (94/121) patients had CIN history,15.7％ of which (19/121) had a history of cervical cancer; 27.3％ (33/121) with hysterectomy history,of which 75.8％ (25/33) was due to cervical intraepithelial neoplasia and cervical cancer resection of the uterus.Papilloma viral load in patients with VAIN Ⅰ was detected with a (HPV-HC2) positive rate of 87.8％ (36/41),while in VAIN Ⅱ and VAIN [patients,the positive rate was 100％,the infection of high risk type of HPV ratio was 95.9％ (116/ 121).of which the mixed infection was accounted for 56.9％ (66/116).There was 94.6％ in oatients with VAIN (114/121),and liquid-based cytology test results suggest abnormality; 88.4％ (107/121) in patients with VAIN lesions occurred in upper 1/3 of vaginal.After diagnosed cervical disease,the onset time of VAIN was (3.45 ± 2.02) years,non-total-hysterectomy was (3.24 ± 1.97) years,after hysterectomy to pathological diagnosis time for VAIN was (5.01 ± 3.95) years:for the diagnosis of VAIN disease after resection of cervical original time was (3.96 ± 2.99) years,and rather than non cervical disease resection was an average of (9.94 ±5.37) years.In the course of treatment of the 12l cases of CIN patients,6 cases of patients were progressed to invasive carcinoma.Conclusions The clinical manifestation of VAIN is similar to CIN,the principle of diagnosis and treatment of CIN can also apply to VAIN,high risk HPV infection virus,cervical intraepithelial neoplasia are the risk factors for VAIN.

BACKGROUND:Carotid artery stenting is a minimal y invasive, safe, simple intervention, which is particularly meaningful to patients with carotid artery stenosis who exhibit poor physical basis and cannot tolerate open surgery. ＜br> OBJECTIVE:To explore the clinical effect of percutaneous transluminal angioplasty and stenting for treatment of extracranial carotid artery stenosis. ＜br> METHODS:Twenty-nine patients with extracranial carotid artery stenosis, including 19 males and 10 females, age ranging from 45-78 years, were enrol ed and subjected to stenting and percutaneous angioplasty. American National Institutes of Health Stroke Scale, modified Rankin Scale and CT examination were tested before treatment and 24 hours after treatment. After 3-12 months fol ow-up, the coagulation indexes and B ultrasound of the carotid artery were reviewed. ＜br> RESULTS AND CONCLUSION:One patient exhibited the complete bifurcation occlusion of the right common carotid artery, and only the diameter of external carotid artery recovered 50.0%. The remaining patients were confirmed by angiograph that completely restored to normal levels, and the success rate was 97%. At 24 hours after treatment, al the 29 patients exhibit significant improvement in the diameter of lesioned carotid artery, scores on American National Institutes of Health Stroke Scale and modified Rankin Scale (P＜0.01, P＜0.05). During the 3-12 months fol ow-up, no cerebral infarction and transient cerebral ischemia occurred and carotid artery stenosis was improved in al patients. At 6 months after operation, one patient had restenosis, and the restenosis rate was 3%. Percutaneous angioplasty combined with biological material stenting for treatment of extracranial carotid artery stenosis can obtain good clinical effects.

As a safe and noninvasive therapy, repetitive transcranial magnetic stimulation (rTMS) and theta burst stimulation (TBS) are used to change the cortical excitability, and induce or enhance neural plasticity, which can promote the recovery of motor function after stroke. This paper summarized the individual treatment of rTMS on motor function of stroke patients, the advantages of TBS compared with rTMS, and the mechanisms and clinical application of TBS.