Objective To examine the effect of lutein supplementation on visual function in people with long-term display light exposure. Method Thirty-seven persons (age 22-30) with long-term display light exposure were divided into low-dose group, high-dose group, and placebo group involving supplementation with 6 mg lutein/d, 12 mg lutein/d, and placebo for 12 w. The levels of serum lutein and visual function indices such as break-up time (BUT), critical flicker fusion frequency (CFF), visuognosis persistence, and reaction time were measured at week 0 and 12. Results After lutein supplementation serum concentrations of high-dose group and low-dose group increased from (0.328?0.120) ?mol/L to (0.733?0.354) ?mol/L, and from (0.356?0.117) ?mol/L to (0.607?0.176) ?mol/L respectively. There was significant increase of break up time in left eyes of high-dose group. Visuognosis persistence was significantly improved, and significant decrease of simple reaction time was observed in high-dose group. No statistical change was observed in critical flicker fusion frequency, discriminative reaction time and choice reaction time during the study. Conclusion Visual function indices such as break-up time, visuognosis persistence and simple reaction time in people with long-term display light exposure who received lutein supplements were significantly improved.

Objective:To evaluate the antioxidant status in IGT and newly diagnosed type 2 diabetes patients, we detected the levels of serum Vitamin E(Vit E), vitamin C(Vit C) and intraerythrocytic glutathion(GSH).Methods:The levels of serum Vit E, Vit C and intraerythrocytic GSH were measured in 32 IGT and 41 newly diagnosed type 2 diabetes patients, and the associated factors with GSH/GSSG (oxidized glutathione) were evaluated.Results:Significant decrease of serum Vit C intraerythrocytic GSH and GSH/GSSG were noted in IGT and newly diagnosed type 2 diabetes patients. There were negative correlation between GSH/GSSG and blood glucose, triglyceride, HbA_(1c) in IGT and newly diagnosed type 2 diabetes patients.Conclusion:The results showed that antioxidant defenses are decreased in IGT and newly diagnosed type 2 diabetes patients. To improve the metabolism of blood glucose and triglyceride may have some beneficial effects on antioxidant defenses in IGT and newly diagnosed type 2 diabetes patients.

BACKGROUND:Sirolimus and Paclitaxel-eluting stents are commonly used for clinical application.Sirolimus-eluting stent have been proved safely and effectively to treat acute myocardial infarction.However,the comparison between those two eluting stents has been less reported yet.OBJECTIVE:To compare the security and long-term efficacy between Sirolimus- and Paclitaxel-eluting stents to the treatment of ST-segment acute myocardial infarction via percutaneous transluminal coronary intervention.METHODS:A total of 354 patients with ST-segment acute myocardial infarction,including 259 males and 95 females,were administrated with Sirolimus- and Paclitaxel-eluting stents.All cases were randomly divided into Sirolimus-elutin9 stent group (n=213) and Paclitaxel-eluting stent group (n=141).Major adverse cardiovascular events were compared between the two groups during 1-year following up.RESULTS AND CONCLUSION:One-year following up indicated that there was no significant difference in recurrent myocardial infarction (1.5% vs.1.5%) and cardiac death (2.5% vs.3.0%) between the two groups.Radiography showed that there was no significant difference in restenosis rate (5.0% vs.4.5%) between the two groups.Inner diameter lose was (0.19±0.34) mm in the Sirolimus-eluting stent group and (0.19±0.37) mm in the Paclitaxel-eluting stent group,and there was no significant difference.Additionally,there was also no significant difference in major adverse cardiovascular events between the two groups (8.9% vs.9.1%,P>0.05),suggesting that both Sirolimus-and Paclitaxel-eluting stents were safe and effective to the treatment of ST-segment acute myocardial infarction via percutaneous transluminal coronary intervention.

Objective To discuss progress in Qinghai Tibetan Thymidylate synthase enhancer in patients with gastric cancer (TSER),ERCC1 polymorphism of genes,gene expression and mRNA studies.Methods 210 cases of advanced gastric cancer were enrolled as required to complete the treatment and follow-up.TSER,ERCC 1 gene polymorphism were tested using PCR-RFLP method.Analysis of gastric cancer is based on the results of gene polymorphism and clinical pathological factors in relations.The expression of ERCC1 mRNA was detected by RTPCR,and tracking access after treatment.Analyzing the relationship among ERCC1 mRNA expression,clinical treatment of overall survival,total response rates and gene polymorphism.Results In patients with advanced gastric C/C in Qinghai ERCC1 gene frequency was 54.2％,C/T frequency was 42.7％,T/T ERCC1 gene frequency was 5.6％.ERCC1 mRNA expression and clinical effect in the treatment of pathological factors were not related,ERCC1 mRNA and gene ERCC1 polymorphism had no relevance.Conclusions Progress in Qinghai Tibetan TSER gene is highly expressed type 3R/3R in patients with gastric cancer.ERCC 1 gene polymorphisms with type C/ C is overexpressed.ERCC1,TSER gene polymorphism and clinicopathologic parameters in advanced gastric is not related.Extension of Tibetan ERCC1 mRNA in gastric cancer patients expression has no association with ERCC1 gene polymorphism.

Ⅳ, change of phosphocreatine (PCr) content was very similar to thatof ATP. Content of ADP, AMP, Cr and HYPO was negatively correlated to ATP content. NAD content was also reduced. In summary, recently proposed hyperkalemic crystalloiddilluted blood protecting myocardium would not be sufficient enough and further improve-ment is quiet neccessary.

Objective To explore the chronic effect of low levels of 2,3,7,8-tetrachlorodibenzo-p-dioxin(TCDD) exposure on the protein expression profile in the serum of rats and to get the further understanding of the molecular mechanism of TCDD toxicity.Methods Thirty-two male SD rats were randomly divided into three TCDD exposed groups treated with TCDD at the doses of 875,350 and 140 ng /kg by gavage,once a week,for 29 consecutive weeks respectively and one control group treated with single corn oil.The proteins in the serum were separated and analyzed by two-dimensional gel electrophoresis and mass spectrometry,the histopathological examination of livers was performed at the same time.Results TCDD induced significant fatty degeneration and necrosis in the livers.Several interesting volume-altered protein spots were identified.Among these proteins,Bal-647 was down-regulated,apolipoprotein E was up-regulated by TCDD and fetuin-like protein was only expressed in the group of 875 ng/kg.Conclusion The differentially expressed proteins may contribute to a better understanding of the toxicity induced by TCDD and may be used as the biomarkers of effect for TCDD exposure.

Parkinson's disease (PD) is a complicated disease, commonly diagnosed among the elderly, which leads to degeneration of the central nervous system. It presently lacks an effective therapy for its complex pathogenesis. Adverse effects from Western drug-based medical intervention prevent long-term adherence to these therapies in many patients. Traditional Chinese medicine (TCM) has long been used to improve the treatment of PD by alleviating the toxic and adverse effects of Western drug-based intervention. Therefore, the aim of this study is to evaluate the efficacy and safety of Xifeng Dingchan Pill (XFDCP), a compound traditional Chinese herbal medicine, taken in conjunction with Western medicine in the treatment of PD patients at different stages in the progression of the disease.

OBJECTIVE To evaluate the inhibitory effect of HPSE AS-ODN on the invasiveness of human Hep-2 cell lines. METHODS HPSE AS- ODN which was complementary with initiation codon region of HPSE mRNA was designed and synthesized. After embedded by cation lipofectin, it was transfected into Hep-2 cells of human laryngocarcinoma. The expression of HPSE protein and HPSE mRNA in Hep-2 cell lines were detected by flow cytometry and RT- PCR. Meanwhile Matrigel invasive assay was used to measure the inhibitory effect of HPSE AS-ODN on the invasiveness of human Hep-2 cell lines. RESULTS The HPSE protein and HPSE mRNA expression and invasiveness of human Hep-2 cells treated with AS- ODN of different concentrations were significantly decreased as the AS-ODN concentration increasing. There was a significantly difference between control group and each group of AS-ODN respectively (P

Objective The purpose of this study was to investigate the effect of isoflurane on baroreflex sensitivity ( BRS ) during induced hypotension and to illucidate the mechanism of isoflurane-induced hypotension. Methods Fifteen female ASA I or II patients aged 13-16 yrs undergoing scoliosis surgery were studied. The patients were unpremedicated. Radial artery and internal jugular vein were cannulated for BP and CVP monitoring. EGG, BP and SpO2 were continuously monitored before and during anesthesia. Anesthesia was induced with midazolam 0.04 mg? kg-1 , fentanyl 2 ?g? kg-1 and propofol 2 mg? kg-1 . Tracheal intubation was facilitated with rocuronium 1 mg?kg-1 . Anesthesia was maintained with isoflurane inhalation and fentanyl infusion (0.4?g?kg-1?h-1). Nitroglycerin (NTG) was infused at 1 ?g?kg-1 ?min-1 at the beginning of surgery to control MAP at 55-65 mm Hg and esmolol 20 mg was given i. v. to control HR 0.8. BRS was measured before induction of anesthesia (T1), immediately after intubation (T2), when end-tidal concentration of isoflurane reached 0.7% (T3 , supine) (T4 , prone), when end-tidal concentration of isoflurane reached 1.3% (T5) , at 30 min after NTG infusion and esmolal bolus injection were stopped (T6) when end-tidal concentration of isoflurane was decreased to 0.7 % (prone, T7 ) and immediately after the patients were turned supine (T8 ) . Results When end-tidal isoflurane concentration reached 1.6%, the desired level of hypotension could be maintained without NTG infusion and esmolol injection. BRS gradually decreased with increasing end-tidal isoflurane concentration. BRS was the lowest at 30 min after termination of NTG infusion and esmolol bolus injection. Conclusion Isoflurane depresses BRS and BRS depression may be involved in the mechanism of isoflurane-induced hypotension.

Regulation of liver regeneration is a complex pathophysiological process, accompanied by the participation of a large number of cytokines, such as the early stage of IL-6, TNF-α, the proliferation phase of HGF, TGFα, as well as the termination phase of TGFβl, and so on. These factors are coordinated in an orderly adjustment of liver cell proliferation. Enhancing the capacity of liver regeneration after liver resection and transplantation, study on molecular mechanism of liver regeneration is of great significance. This article summarizes liver regenerati after partial hepatectomy and the role of the relevant molecules.