We report here on an 11-year-old child with a pustulovesicular eruption on her whole body, including her palms, soles and oral mucosa, and this was accompanied with fever. A serologic test was positive for IgM varicella zoster antibody and coxsackievirus A16 antibody. The histopathologic examination from the palm revealed intraepidermal pustules that showed neither inclusion bodies nor multinucleated giant cells. We made the final diagnosis, according to the serologic tests and histopathologic findings, as varicella combined with hand-foot-mouth disease caused by coxsackievirus A16 in a previously healthy child
Chickenpox ; Child ; Fever ; Giant Cells ; Herpes Zoster ; Humans ; Immunoglobulin M ; Inclusion Bodies ; Mouth Mucosa ; Serologic Tests

Focal segmental glomerulosclerosis (FSGS) is the name of the primary glomerular disease as well as the terminology to describe the secondary phenomena of any other glomerular diseases. It is characterized by sclerosis, hyalinosis, foam cell infiltration, vacuolar change of podocytes, and halo formation in the glomerulus. Throughout the interstitium, lymphocytes infiltration, tubular atrophy and vascular changes are accompanied. Occasionally, IgM and/or C3 depositions are noted in the sclerotic areas. Electron microscopically, diffuse effacement of foot processes are seen in non-sclerotic area like minimal change disease. Podocyte injury patterns including vacuolar changes are frequently examined. Recently, Columbia group has suggested morphologic classification of FSGS and they demonstrated very good prognosis of tip lesion and poor prognosis of both collapsing and cellular types. However, the pathogenetic classification has been suggested by others; hyperfilteration, podocyte injury, genetic lesions etc. Further studies are necessary to understand and treat this disease.
Atrophy ; Electrons ; Foam Cells ; Foot ; Glomerulosclerosis, Focal Segmental ; Immunoglobulin M ; Lymphocytes ; Nephrosis, Lipoid ; Podocytes ; Prognosis ; Sclerosis

Extramedullary plasmacytoma is a relatively rare tumor, occuring in a wide variety of organs and tissues, and is most frequently seen in the oral cavity and upper respiratory tract. Solitary extramedullary plasmacytoma located in the gastrointestinal tract is rare, especially the in colon. We report a case of solitary extramedullary plasmacytoma in the cecum of a 58-year-old man which simulated a carcinoma both endoscopically and radiologically. But histopathologic evaluation demonstrated a sheet-like proliferation of pure plasma cells with monoclonality for IgM and lamda chain which confirmed the diagnosis of plasmacytoma.
Cecum ; Colon* ; Diagnosis ; Gastrointestinal Tract ; Humans ; Immunoglobulin M ; Middle Aged ; Mouth ; Plasma Cells ; Plasmacytoma* ; Respiratory System

Waldenstr's macroglobulinemia is a slowly progressing disease characterized by proliferation of small lymphocytes, plasmacytoid lymphocytes and plasma cells in the bone marrow with increased serum monoclonal IgM. Since 1944, only a few cases of Waldenstr's macroglobulinemia with pulmonary involvement have been described. We experienced a case of Waldenstr's macroglobulinemia with pulmonary involvement. The patient was 66-year-old male complaining dyspnea. His cytological finding of pleural fluid aspirates revealed many plasmacytoid lymphocytes. Serum electrophoresis showed the sharp M-peak in the gamma globulin region, which was identified as a IgM, kappa type by immunofixation. Bone marrow findings showed small lymphocytic and plasmacytoid lymphocytic infiltration.
Aged ; Bone Marrow ; Dyspnea ; Electrophoresis ; gamma-Globulins ; Humans ; Immunoglobulin M ; Lymphocytes ; Male ; Plasma Cells ; Waldenstrom Macroglobulinemia*

To prove the buffering contribution of mitochondria to the increase of intracellular Ca2+ level ([Ca2+]i) via background nonselective cation channel (background NSCC), we examined whether inhibition of mitochondria by protonophore carbonylcyanide m-chlorophenylhydrazone (CCCP) affects endothelial Ca2+ entry and Ca2+ buffering in freshly isolated rabbit aortic endothelial cells (RAECs). The ratio of fluorescence by fura-2 AM (R340/380) was measured in RAECs. Biological state was checked by application of acetylcholine (ACh) and ACh (10microM) increased R340/380 by 1.1+/-0.15 (mean+/-S.E., n=6). When the external Na+ was totally replaced by NMDG+, R340/380 was increased by 1.19+/-0.17 in a reversible manner (n=27). NMDG+-induced [Ca2+]i increase was followed by oscillatory decay after [Ca2+]i reached the peak level. The increase of [Ca2+]i by NMDG+ was completely suppressed by replacement with Cs+. When 1microM CCCP was applied to bath solution, the ratio of [Ca2+]i was increased by 0.4+/-0.06 (n=31). When 1microM CCCP was used for pretreatment before application of NMDG+, oscillatory decay of [Ca2+]i by NMDG+ was significantly inhibited compared to the control (p < 0.05). In addition, NMDG+-induced increase of [Ca2+]i was highly enhanced by pretreatment with 2microM CCCP by 320+/-93.7%, compared to the control (mean+/-S.E., n=12). From these results, it is concluded that mitochondria might have buffering contribution to the [Ca2+]i increase through regulation of the background NSCC in RAECs.
Acetylcholine ; Baths ; Carbonyl Cyanide m-Chlorophenyl Hydrazone ; Endothelial Cells* ; Fluorescence ; Fura-2 ; Mitochondria*

Humans ; M Cells ; Cell Line ; Liver Neoplasms ; Cholesterol ; Cell Line, Tumor ; Apoptosis ; Cell Cycle Checkpoints

In order to achieve large-scale production of HSV-IgM (HSV1, HSV2) human-mouse chimeric antibody in vitro, the gene sequence of the corresponding hybridoma cell was harvested by RNA ligase-mediated rapid amplification of cDNA ends (RLM-RACE) technique to clone the chimeric antibody into eukaryotic expression vectors, and express the target proteins in CHO-S cells. At the same time, the screening process of stable cell lines was optimized, and the pressure conditions of pool construction stage and monoclonal screening stage were explored. Finally, the target protein was purified by protein L affinity purification method and the biological activity was detected. The recombinant IgM antibodies, HSV1 and HSV2, weighted at 899 kDa and 909 kDa respectively, were prepared. The optimal screening pressure was 20P200M (the first phase of pressure) and 50P1000M (the second phase of pressure). The final titer for the monoclonal expression of HSV1-IgM and HSV2-IgM was 1 620 mg/L and 623 mg/L, respectively. This study may facilitate the development of quality control products of HSV1 and HSV2 IgM series recombinant antibodies as well as efficient expression of IgM subtype antibodies in vitro.
Cricetinae ; Humans ; Animals ; Mice ; Immunoglobulin M/genetics* ; Antibodies, Viral ; CHO Cells ; Cricetulus ; Hybridomas ; Recombinant Fusion Proteins

Idiopathic granulomatous mastitis (IGM) is a rare chronic inflammatory condition of unknown etiology. It occurs in young, parous women who had experienced breast feeding, and is manifested as a tender breast mass. Histologically, a discrete granulomatous lobulitis consisting of giant cells, neutrophils, epitheloid cells, and foamy macrophges is shown. Extramammary manifestations of IGM are rare. Erythema nodosum (EN), arthralgia, and episcleritis have been reported, supporting the theory that there is an autoimmune component in the etiology of IGM. Coincidence of IGM and EN is rare. Herein, we report a 28-year-old pregnant woman in whom granulomatous mastitis presented with arthralgia and EN.
Adult ; Arthralgia ; Breast ; Breast Feeding ; Erythema ; Erythema Nodosum ; Female ; Giant Cells ; Granulomatous Mastitis ; Humans ; Immunoglobulin M ; Neutrophils ; Pregnant Women ; Scleritis

CMV is a largest member of herpes viridae consisted of 230-240 Kb double stranded DNA, 20 protein matrix and envelope. It has specific replication cycle with 3 phase. Human immunity against CMV, cellular immune and humoral immune are envolved and specially CD + 8 cytotoxic cell and natural killer cell are important because they controll primary infection, recurrent infection and latent infection. Our patient was 2 month old, healthy female infant. She had acute pneumonia and then progressed fulminent respiratory failure, cardiomegaly, hydrocephalus and DIC. At first, we tried to find cause of this condition but couldn't anything. We performed several CMV serologic study and on hospital day 30th, Anti-CMV IgM was positive and increased IgG. And we report the case of CMV pneumonia in a healthy infant.
Cardiomegaly ; Cytomegalovirus* ; Dacarbazine ; DNA ; Female ; Humans ; Hydrocephalus ; Immunoglobulin G ; Immunoglobulin M ; Infant* ; Killer Cells, Natural ; Pneumonia* ; Respiratory Insufficiency

The authors examined human immunity indicators such as CD4, CD8, Natural Killer (NK) cell, IgG, IgA, and IgM, and heavy metals such as Pb, Cr, Cd to evaluate the health hazard of welders. We selected total 126 male subjects, 60 welders who had more than 5 year working history as an exposure group, and 66 office workers and medical college students as a control group. This immunity evaluation approach is the first newly designed study that has never been tried in past studios on welder's heath evaluation. And the results were as follows. 1. Total lymphocyte count of peripheral blood was significantly higher in welder group, 2,615+/-650 ea/microliter compared with 2,368+/-681 ea/microliter in control group (p<0.05). 2. The proportion of CD4, CD8, NK cell in welder group was 37 5+/-9.4%, 24.0+/-8.8%, and 21.0+/-9.5%, respectively, and 35.5+/-6.5%, 25.0+/-6.9%, and 19.6+/-8.8% in control group. CD4 and NK cell were slightly higher, and CD8 was slightly lower in welder group. But there was no statistical significance between two groups (p> or =0.05). 3. IgG, IgA, and IgM in welder group were 15.9 mg/dl, 2.7 mg/dl, and 1.5 mg/dl, respectively, and 15.6 mg/dl, 2.9 mg/dl and 1.7 mg/dl in control group. There was no statistical significance between two groups(p> or =0.05). 4. Blood lead, cadmium and chromium concentrations were 4.2+/-1.8 microgram/dl, 0.4+/-0.38 microgram/dl, 1.2+/-0.6 microgram/dl in welder group and 3.2+/-1.7 microgram/dl, 0.44+/-0.29 microgram/dl, 1.1+/-0.6 microgram/dl in control group, respectively, and these value were within normal ranges.
Cadmium ; Chromium ; Humans ; Immunoglobulin A ; Immunoglobulin G ; Immunoglobulin M ; Killer Cells, Natural ; Lymphocyte Count ; Male ; Metals, Heavy ; Reference Values