Objective To explore the expression of the CENP-W in gliomas and investigate the effects of its invasion. Methods The expression level of the CENP-W in gliomas with varied pathologic grade were detected by immunohistochemical analysis,RealTime PCR,and Western Blotting. U251 cells were transfected with the specific siRNA to repress the CENP-W expression level. The invasion ability of U251 cells were examined by Transwell Chamber assay ,while RAS mRNA and protein levels were detected at the same time. Results The expression levels of the CENP-W in glioma tissues were significantly high and the CENP-W gene could enhance the invasion of U251 cells . The expression of RAS was down-regulated when the expression of CENP-W was repressed. Conclusion The CENP-W has an oncogenic role in human brain gliomas and may regulate the invasion of gliomas by adjusting the RAS signaling pathways.

Objective To understand the residual levels of eugenol in aquatic products sold in 4 cities in Hubei Province and timely discover potential food safety hazards, and to provide a scientific basis for supervision of eugenol in aquaculture and transportation of aquatic products. Methods From 2021 to 2023, 124 samples of aquatic products were randomly collected from supermarkets and farmers’ markets in Yichang, Xiantao, Jingmen, and Ezhou cities in Hubei Province. The ultra-high performance liquid chromatography-mass spectrometry was used to detect the residues of eugenol. Results Eugenol was detected in 51 out of 124 samples, with a detection rate of 41.13%，and a concentration range of N.D. - 2601μg/kg. Among them, 19 out of 40 samples were detected in 2021, with a detection rate of 47.50%; 12 out of 40 samples were detected in 2022, with a detection rate of 30.00%; and 20 out of 44 samples were detected in 2023, with a detection rate of 45.45%. There was no statistically significant difference in the detection rates in different years (P>0.05). The detection rates of eugenol in aquatic products sold in Yichang City, Xiantao City, Jingmen City, and Ezhou City were 22.58%, 35.48%, 41.94%, and 64.52%, respectively, and the differences were statistically significant (P<0.05). The detection rates of samples in supermarkets and farmers' markets were 35.56% and 44.30%, respectively, and the difference was not statistically significant (P>0.05). The detection rate of eugenol in bighead carp was the highest at 66.67%, followed by grass carp with a detection rate of 61.22%, which was significantly higher than other fish (P<0.05). Conclusion At present, the widespread use and arbitrary addition of eugenol should be paid attention to by relevant departments.