Objective To systematically review the prednisone-induced test results for the prognosis assessment of acute lymphoblastic leukemia(ALL) in children.Methods Based on the established inclusion and exclusion criteria,studies of prednisone-induced test in evaluating the prognosis of childhood ALL were electronically searched from January 1990 to November 2016 from PubMed,Embase,The Cochrane Library,WanFang,VIP and CNKI data-base.Two independent researchers browsed literature,extracted data and evaluated the literature quality,Meta-analysis was performed by using RevMen5.3 software.Results A total of 17 articles were included.Meta analysis showed that after completing prednisone induced test in children,the 5 year-event-free survival (5 y-EFS) and 8 y-EFS of good response to prednisone (PGR) was better than that of poor response(PPR) group to prednisone [mean deviation (MD) =22.03,95 ％ CI:18.87-25.18,P ＜ 0.000 01;MD =27.90,95 ％ CI:20.94-34.85,P ＜ 0.000 01].The continuous complete remission rate and recurrence rate in 2 groups were compared,and the continuous complete remission of PGR group was high [odds ratio (OR) =4.82,95 ％ CI:2.81-8.29,P ＜ 0.000 01] and the recurrence rate was low (OR =0.26,95 ％ CI:0.11-0.59,P =0.001),and all the differences were statistically significant.There were statistical significance in T cell immune typing,the initial white blood cell count and at day 19(D19) bone marrow(M1,M2) between 2 groups [relative risk (RR) =0.30,95 ％ CI:0.20-0.44,P ＜ 0.000 01;OR =0.11,95 ％ CI:0.08-0.16,P ＜0.000 01;OR =8.53,95％ CI:2.85-25.52,P =0.000 1;OR =0.12,95％ CI:0.03-0.39,P =0.000 5].There was no statistical significance in gender(male) and D19 bone marrow(M3) between 2 groups (OR =0.89,95％ CI:0.62-1.28,P=0.53;OR=0.24,95％CI:0.04-1.44,P=0.12).Conclusion The prednisone induction test is an important index to evaluate the prognosis of children with ALL,and the treatment of prednisone induced test based on selection can improve the prognosis of children.

Objective:To investigate the effect and potential mechanism of serum exosome-derived cirC_0009362 on the osteogenic differentiation of human bone marrow mesenchymalstem cells (hBMSCs) .Methods:Serum samples from patients with osteoporosis (OP) were collected and exosomes were isolated. The expression level of circ_0009362 in exosomes was detected by qRT-PCR. hBMSCs osteogenesis was induced and the expression of circ_0009362 and miR-29b-3p was detected. The interaction between circ_0009362 and miR-29b-3p was detected by dual luciferase reporter assay. Alkaline phosphatase (ALP) kit was used to detect ALP activity and alizarin red (ARS) staining was used to detect calcium deposition.Results:Compared with healthy control group, the expression of circ_0009362 in serum exosomes of OP patients was increased, and the expression of circ_0009362 was decreased after inducing hBMSCs osteogenesis (all P<0.05) . The ALP activity and the percentage of calcium deposition in hBMSCs were decreased by exosomes, and this effect was achieved by secreting circ_0009362. The effect of exosomes was partially offset by circ_0009362 expression in knockdown exosomes (all P<0.05) . The expression of miR-29b-3p was increased after inducing hBMSCs osteogenesis ( P<0.05) . Circ_0009362 had a targeting relationship with miR-29b-3p, and exosomes inhibited the expression of miR-29b-3p by secreting circ_0009362. The ALP activity and the percentage of calcium deposition in hBMSCs were promoted by overexpression of miR-29b-3p, which was partially offset by exosomes (all P<0.05) . Conclusion:Serum exosomes of OP patients inhibit the osteogenic differentiation of hBMSCs by secreting circ_0009362 to down-regulate the expression of miR-29b-3p.

BACKGROUND:Rotator cuff muscle degeneration(muscle atrophy,fibrosis and fatty infiltration)is a common condition after rotator cuff tears,which seriously affects shoulder function and surgical outcomes.Ginsenoside Rg1 has biological effects such as anti-oxidation,anti-apoptosis and lipid-lowering.However,the effect of ginsenoside Rg1 on muscle degeneration after rotator cuff tear has not been reported. OBJECTIVE:To investigate the effect of ginsenoside Rg1 on muscle degeneration after massive rotator cuff tear in mice. METHODS:Sixty C57BL/6J mice were randomly divided into sham group,model group,ginsenoside Rg1 low dose group and ginsenoside Rg1 high dose group,with 15 mice in each group.The skin of the right shoulder of mice in the sham group was cut and sutured.Massive rotator cuff tear mouse models of the right shoulder were established in the other three groups.Supraspinatus tendon and suprascapular nerve compression were administrated.Mice in the sham and model groups were intraperitoneally injected with 0.5 mL of saline after operation,while those in the ginsenoside Rg1 low and high dose groups were intraperitoneally injected with ginsenoside Rg1 30 and 60 mg/kg respectively,once a day,for 6 weeks.Mice were assessed for limb function by gait analysis the day after the last injection.After euthanasia,the supraspinatus muscle on the operated side was taken to measure the muscle atrophy rate and muscle contractility.Muscle tissue was stained with oil red O and Masson.RT-PCR was used to detect the expression of atrophy,fibrosis,and fatty infiltration related genes. RESULTS AND CONCLUSION:Compared with the model group,low-and high-dose ginsenoside Rg1 significantly increased paw print area and step length(P<0.05).Compared with the model group,low-and high-dose ginsenoside Rg1 significantly increased myofiber cross-sectional area and supraspinatus contractility(P<0.05),and significantly decreased wet muscle mass reduction ratio,fatty infiltration area ratio,and collagen fiber area ratio(P<0.05).Compared with the model group,low-and high-dose ginsenoside Rg1 significantly decreased the expression of atrophy,fibrosis,and fatty infiltration related genes(P<0.05).There was no significant difference in paw print area,supraspinatus muscle contractility,and myofiber cross-sectional area between ginsenoside Rg1 low and high dose groups(P>0.05),and all other indexes were better in the ginsenoside Rg1 high dose group than in the ginsenoside Rg1 low dose group(P<0.05).To conclude,ginsenoside Rg1 could significantly reduce muscle atrophy,fibrosis and fatty infiltration following massive rotator cuff tear in mice,which is beneficial to improve muscle strength and limb function.