Human T lymphocytes are divided into αβT cells and γδT cells on the basis of the different expressions of T cell receptors. In recent years, the studies of the regulation of T cell activation and tolerance by co-stimulatory molecules and their signaling pathways in αβT cell have made remarkable progress; however, relatively fewer investigations have been performed on γδT cells. A clearer understanding of the roles of co-stimulatory molecules and their signaling pathways in the positive/negative regulation of γδT cells at different stages will provides new insights for the treatment of viral infections, cancer, autoimmune diseases, transplant rejection, and other conditions.
Humans ; Lymphocyte Activation ; physiology ; Signal Transduction ; T-Lymphocyte Subsets ; physiology

OBJECTIVETo study the postnatal changes in lymphocyte subsets in early preterm infants and the effect of perinatal factors on lymphocyte subsets.

METHODSA total of 61 early preterm infants were enrolled. Flow cytometry was used to measure the absolute counts of lymphocytes and lymphocyte subsets at 1, 7, 14, and 28 days after birth, as well as at 6 months after birth for 17 of these early preterm infants. The effects of perinatal factors, such as antepartum use of hormone, intrauterine infection, gestational age at birth, and Ureaplasma urealyticum (UU) colonization, on lymphocyte subsets were analyzed.

RESULTSThe absolute counts of lymphocyte subsets except natural killer (NK) cells were lowest at birth, increased rapidly at 1 week after birth, and reached the levels in healthy infants at 6 months; the count of NK cells remained at a low level and increased significantly at 6 months after birth. Compared with those with a gestational age of <28 weeks, the early preterm infants with a gestational age of ≥28 weeks had significantly higher absolute counts of T cells, T helper (Th) cells, and NK cells at 7 days after birth, a significantly higher absolute count of T cells at 14 days after birth, and significantly higher absolute counts of lymphocytes and Th cells at 28 days after birth (P<0.05). Compared with the group not using hormone, the group using hormone showed a significantly higher absolute count of T cells at 7 days after birth and significantly higher absolute counts of lymphocytes and all subsets at 14 days after birth (P<0.05). There was no significant difference in lymphocyte subsets at 1 day after birth between the intrauterine infection and non-infection groups (P>0.05); the intrauterine infection group had significantly higher absolute counts of B cells at 7 and 14 days after birth than the non-infection group. Compared those without UU colonization, the infants with UU colonization had significantly higher absolute counts of lymphocytes, T cells, Th cells, and Ts cells at 1 day after birth and a significantly higher absolute count of B cells at 14 days after birth.

CONCLUSIONSEarly preterm infants have deficiencies in innate immune cells at birth and normal levels at about 6 months after birth. Various perinatal factors including antepartum use of hormone, gestational age at birth, intrauterine infection, and UU colonization have long-term effects on lymphocyte subsets in early preterm infants.

Female ; Humans ; Infant, Newborn ; Infant, Premature ; immunology ; Lymphocyte Subsets ; microbiology ; physiology ; Male ; Ureaplasma urealyticum ; isolation & purification

OBJECTIVETo investigate the changes in white blood cell populations, lymphocyte subsets and stress-related cytokines after long-term exercise training and address the association between blood cell changes and stress-related cytokines in relation to exercise.

METHODSA total of 1038 professional athletes were examined for CBC with Sysmex XE2100, and the T, B, and NK lymphocyte subsets were analyzed with flow cytometry. The testees' RNA were extracted from 1 ml whole blood, and the stress-related cytokines such as CRP, SELL,TNF-α, IL8, IL4, ICAM1, PECAM1, IL6, and NOS were tested by multi-RT-PCR and fragments separated by capillary electrophoresis using Beckman Coulter GeXP system.

RESULTSNo obvious difference was found in WBC count between the athletes, all within normal range. The proportion of lymphocytes was increased in the athletes by 20%-40% in comparison with the normal level, and the CD3+, CD3+CD4+, and CD3+CD8+ T, B, and NK lymphocyte subsets were all lower in the athletes than the normal range. The cytokine expressions exhibited no significant gender-related difference. IL-8, TNF-α and SELL expressions increased while IL-4 decreased in the athletes. Correlations were noted between the changes of the cells and the cytokine expressions.

CONCLUSIONLong-term exercise training affects the immune system and cause stress, which may potentially increase the risks of some chronic diseases.

Adolescent ; Adult ; Athletes ; Cytokines ; physiology ; Exercise ; physiology ; Female ; Flow Cytometry ; Humans ; Immunologic Factors ; physiology ; Lymphocyte Count ; Lymphocyte Subsets ; cytology ; immunology ; physiology ; Male ; Stress, Physiological ; immunology ; Young Adult

CD4 Antigens ; immunology ; Humans ; Interleukin-2 Receptor alpha Subunit ; immunology ; T-Lymphocyte Subsets ; immunology ; physiology ; T-Lymphocytes, Regulatory ; physiology

The aim of this study was to explore effect of CD8+CD28- T-lymphocyte in the inhibition of mesenchymal stem cells (MSC) on T-lymphocyte proliferation. T cells were harvested by using nylon column and CD8+ T cells were sorted by magnetic beads; the T-lymphocyte proliferation in the presence of PHA was evaluated by MTT; the proportion of CD8+CD28- T cells was assayed by fluorescence-activated cell sorter (FACS). The results showed that MSC inhibited T-lymphocyte proliferation and the inhibitory effect depended on the amount of MSC; the data of FACS indicated that in the CD8+ T cells co-cultured with MSC, CD8+CD28- T cells were up-regulated significantly, compared with the non-treated CD8+ T cells. In conclusion, MSC perform their immunosuppressive function by up-regulation of CD8+CD28- T cells.
Bone Marrow Cells ; physiology ; CD28 Antigens ; analysis ; CD8 Antigens ; analysis ; Humans ; Lymphocyte Activation ; Mesenchymal Stromal Cells ; physiology ; T-Lymphocyte Subsets ; immunology ; Up-Regulation

OBJECTIVETo study the changes of red cell immune function and T-lymphocyte subsets in children with bronchiolitis and their possible roles in the pathogenesis of bronchiolitis.

METHODSForty-five children with bronchiolitis and 30 healthy controls were enrolled. Red cell immune complex rosette (RBC-ICR) and red cell C3b receptor rosette (RBC-C3bRR) were detected. The percentages of CD3+, CD4+ and CD8+ cells were assayed by flow cytometry.

RESULTSRBC-C3bRR［(13.6 ± 6.2)% vs (18.0 ± 7.4)%] and the percentage of CD8+ cells [(21.6 ± 4.4)% vs (25.6 ± 5.2) %］ in the bronchiolitis group were lower than those in the control group (P<0.01). The percentage of CD3+ cells ［(59.9 ± 6.7)% vs (52.1 ± 8.3)%］ and CD4+ cells [(53.5 ± 6.2)% vs (46.8 ± 4.9)%] and RBC-ICR [(8.3 ± 3.5)% vs (6.1 ± 2.5)%] in the bronchiolitis group were higher than those in the control group (P<0.01). The percentage of CD4+ cells was positively correlated with RBC-ICR (r=0.63,P<0.05) and negatively correlated with RBC-C3bRR (r=-0.82,P<0.01).

CONCLUSIONSThere are dysfunctions of red cell immune and T-lymphocyte subsets in children with brochiolitis, which may play a role in the pathogenesis of brochiolitis.

Bronchiolitis ; etiology ; immunology ; Erythrocytes ; immunology ; Female ; Humans ; Infant ; Male ; Receptors, Complement 3b ; physiology ; Rosette Formation ; T-Lymphocyte Subsets ; immunology

OBJECTIVETo identify the frequency and interferon (IFN)-alpha-producing ability of circulating type 2 pre-dendritic cells (pDC2) and evaluate its role in liver cirrhotic patients with chronic HBV infection.

METHODS27 liver cirrhotic patients were included in our study and 25 patients with chronic hepatitis B and 25 healthy individuals were enrolled as controls. The numbers of circulating pDC2 and lymphocytes including CD4+ T cells, CD8+ T cells, NK cells as well as B cells were analyzed by flow cytometry. The IFN-alpha-producing function of peripheral blood mononuclear cells (PBMCs) representing the circulating pDC2 was determined by ELISA assay after stimulated by ultraviolet-inactivated herpes simplex virus-1 (UV-HSV-1).

RESULTSThe number of pDC2 were (7.21+/-2.38)*10(6)/L, (4.49+/-3.08) *10(6)/L and (2.89+/-1.17) *10(6)/L for healthy control, chronic hepatitis B and cirrhotic patients respectively. Both the number and IFN-alpha-producing function of circulating pDC2 in liver cirrhotic patients significantly lower than that in healthy subjects. There was a correlated simultaneous decrease numbers of circulating CD8+ T cells, NK cells in HBV-infected cirrhotic patients. Furthermore, cirrhotic patients with opportunistic infections have lower numbers of pDC2, CD8+ T cells and NK cells compared to those without opportunistic infections.

CONCLUSIONSLiver cirrhotic patients with chronic HBV infection have a significant decrease of circulating pDC2 level and IFN-alpha-producing function. The decreased number and function of pDC2, together with the lower number of CD8+ T cells and NK cells may result in the decline of host immune response, which may partially contribute to the disease progression of HBV infection and opportunistic infections.

Cell Count ; Dendritic Cells ; physiology ; Hepatitis B, Chronic ; immunology ; Humans ; Interferon-alpha ; biosynthesis ; Liver Cirrhosis ; immunology ; T-Lymphocyte Subsets ; immunology

The immunoregulatory effects of mescenchymal stem cell (MSC) and its application have become a hot research topic in recent years. This article reviews the up-to-dated research advances in the features and mechanisms of immune regulation of MSC and its application.
Animals ; Humans ; Lymphocyte Subsets ; immunology ; Mesenchymal Stem Cell Transplantation ; Mesenchymal Stromal Cells ; physiology ; T-Lymphocytes, Regulatory ; immunology

Forest bathing trip is a short, leisurely visit to forest. In this study we determined the health effects of forest bathing trip on elderly patients with chronic obstructive pulmonary disease (COPD). The patients were randomly divided into two groups. One group was sent to forest, and the other was sent to an urban area as control. Flow cytometry, ELISA, and profile of mood states (POMS) evaluation were performed. In the forest group, we found a significant decrease of perforin and granzyme B expressions, accompanied by decreased levels of pro-inflammatory cytokines and stress hormones. Meanwhile, the scores in the negative subscales of POMS decreased after forest bathing trip. These results indicate that forest bathing trip has health effect on elderly COPD patients by reducing inflammation and stress level.
Aged ; Cytokines ; genetics ; metabolism ; Female ; Forests ; Gene Expression Regulation ; physiology ; Humans ; Lymphocyte Subsets ; physiology ; Male ; Middle Aged ; Pulmonary Disease, Chronic Obstructive ; pathology ; psychology ; therapy ; Recreation

In this paper, we review the results of previous studies and summarize the effects of various factors on the regulation of bone metabolism in traumatic bone infections. Infection-related bone destruction incorporates pathogens and iatrogenic factors in the process of bone resorption dominated by the skeletal and immune systems. The development of bone immunology has established a bridge of communication between the skeletal system and the immune system. Exploring the effects of pathogens, skeletal systems, immune systems, and antibacterials on bone repair in infectious conditions can help improve the treatment of these diseases.
Anti-Bacterial Agents/administration & dosage* ; Bone and Bones/metabolism* ; Cellular Microenvironment ; Humans ; Immune System/immunology* ; Lymphocyte Subsets/immunology* ; Osteitis/microbiology* ; Osteoblasts/physiology* ; Osteoclasts/physiology* ; Staphylococcal Infections