OBJECTIVETo provide experimental data for the quality control of processed Paeonia lactiflora, a Chinese herbal medicine.

METHODTraditional processing of P. lactiflora was simulated, content of paeoniflorin and water extracts among different preparations were assayed by HPLC; The quantitative correlations among different processing conditions were analyzed, the effects of processing parameters on the contents of paeoniflorin and water extracts were assayed and analysed.

RESULT AND CONCLUSIONThe controlled processing parameters were correlated with covariables which showed that processing procedures was controllable, and the heating temperature was a factor impacting the content of paeoniflorin.

Caffeic Acids ; analysis ; China ; Chromatography, High Pressure Liquid ; methods ; Ecosystem ; Lycopus ; chemistry ; Plant Components, Aerial ; chemistry ; Plants, Medicinal ; chemistry ; Quality Control ; Reproducibility of Results ; Seasons ; Sensitivity and Specificity

OBJECTIVETo Study the antioxidant activity of Herba Lycopi, and screen the main active fractions.

METHODThe different polar fractions were isolated from the ethanol extract of Herba Lycopi. The scavenging activity to DPPH and superoxide anions, reducing effect on Fe3+, and inhibiting activity of lipid peroxidation of the fractions were studied in vitro by high-throughput screening technique.

RESULTThe ethanol extract, ethyl acetate fraction (LLE-A. E, MCCM2921) and n-buthanol fraction (LLE-A. B, MCCM2922) of Herba Lycopi showed the scavenging activity to DPPH and superoxide anions, reducing effect on Fe3+, and inhibiting activity of lipid peroxidation.

CONCLUSIONThe ethanol extract and different polar fractions showed antioxidant capacity in vitro. That may be explain that the effect of activating blood and dissolving stasis and anti-fibrosis of Herba Lycopi in traditional application. The results may be helpful for further development of Herba Lycopi as a low-toxic antioxidant drug.

Animals ; Antioxidants ; analysis ; pharmacology ; Lipid Peroxidation ; drug effects ; Lycopus ; chemistry ; Male ; Microsomes, Liver ; drug effects ; metabolism ; Plant Extracts ; analysis ; pharmacology ; Rats ; Rats, Wistar

OBJECTIVETo study the effects of L.F04, the active fraction of Lycopus lucidus, on erythrocytes rheological property so as to investigate its mechanism in promoting blood circulation and removing blood stasis.

METHODThe effects of L.F04 (used for treatment for 10 days in different dosages) on deformability, aggregation and membrane liquidity of erythrocytes (MLE) as well as whole blood apparent viscosity (eta(b)) were examined on the basis of rat model of blood-stasis syndrome induced by venous injection of high molecular weight dextran.

RESULTAs compared with the normal control group, the model group's RBC deformability and MLE were lower, and the aggregation of erythrocytes and eta(b) were higher. Compared with the model group, both L.F04 0.612 g/kg and 0.306 g/kg showed significant effect in improving deformability and inhibiting aggregation of red blood cells (RBC) and reducing blood viscosity. The trend of improving MLE was also shown.

CONCLUSIONL.F04 could significantly improve the abnormal rheological property of erythrocytes.

Animals ; Blood Viscosity ; drug effects ; Dextrans ; pharmacology ; Drugs, Chinese Herbal ; administration & dosage ; Erythrocyte Aggregation ; drug effects ; Erythrocyte Deformability ; drug effects ; Hemorheology ; Hemostasis ; drug effects ; Lycopus ; Male ; Rats ; Rats, Wistar ; Space Flight