1.Assessment of In vitro Antioxidant, Antidiabetic and Cytotoxic Activities of Sphaeranthus africanus Extracts
Tran Thi HUYEN ; Julien DUJARDIN ; Nguyen Thi THU HUONG ; Chung Thi MY DUYEN ; Nguyen Hoang MINH ; Ha Quang THANH ; Dao Tran MONG ; Ly Hai TRIEU ; Nguyen Mai TRUC TIEN ; Mai Thanh CHUNG ; Nguyen Nhat MINH ; Nguyen Thi NGOC DAN ; Huynh LOI
Natural Product Sciences 2023;29(2):98-103
Sphaeranthus africanus is commonly used as a traditional remedy for sore throats and pain treatment in Vietnam. The aerial parts have been studied for its anti-inflammatory and anti-proliferative properties. However, the antioxidant and antidiabetic potential of the plant has not been explored. In this work, hydrophilic extracts of the plant's aerial parts were prepared in order to investigate its antioxidant and anti-diabetic properties. Also, the cytotoxicity of the root was evaluated and compared to that of the aerial parts. All of the extracts inhibited lipid peroxidation with IC 50 values ranging from 2.05 to 3.56 µg/mL, indicating substantial antioxidant activity. At an IC 50 value of 4.80 μg/mL, the 50% ethanol extract exhibited the most potent inhibition of α-glucosidase. The cytotoxic activity of root extracts is 2 to 5-fold less than that of the aerial parts. Nevertheless, dichloromethane and ethyl acetate extracts of the root demonstrated a selective effect on leukemia cells, with no harm towards the normal HEK-293 cell line. This work provides a scientific support for the antioxidant and antidiabetic activity of the plant. Hence, it may find a promising material for the development of novel antioxidant and antidiabetic agents. More research can be conducted on the phytochemistry and anticancer activities of the plant’s root.
2.Effect of Diquafosol on Hyperosmotic Stress-induced Tumor Necrosis Factor-α and Interleukin-6 Expression in Human Corneal Epithelial Cells
Yeoun Hee KIM ; In Jun YANG ; Ly Thi Huong NGUYEN ; Sang Il GUM ; Sung YU ; Gwang Ja LEE ; Bo Ae KIM ; Jae Chang JUNG ; Young Jeung PARK
Korean Journal of Ophthalmology 2020;34(1):1-10
Blotting, Western
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Conjunctival Diseases
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Dry Eye Syndromes
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Epithelial Cells
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Fluorescent Antibody Technique
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Humans
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Immunohistochemistry
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Inflammation
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Interleukin-6
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Necrosis
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Phosphorylation
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Real-Time Polymerase Chain Reaction
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RNA, Messenger
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Tears
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Tumor Necrosis Factor-alpha
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Wounds and Injuries
3. Ethanol extracts of Scutellaria baicalensis protect against lipopolysaccharideinduced acute liver injury in mice
Hai Nguyen THANH ; Tuan Anh LE ; Huong Duong Thi LY ; Tung Nguyen HUU ; Loi Vu DUC ; Thu Dang KIM ; Tung Bui THANH ; Hue Pham Thi MINH
Asian Pacific Journal of Tropical Biomedicine 2015;5(9):761-767
Objective: To investigated the protective potential of ethanol extracts of Scutellaria baicalensis (S. baicalensis) against lipopolysaccharide (LPS)-induced liver injury. Methods: Dried roots of S. baicalensis were extracted with ethanol and concentrated to yield a dry residue. Mice were administered 200 mg/kg of the ethanol extracts orally once daily for one week. Animals were subsequently administered a single dose of LPS (5 mg/kg of body weight, intraperitoneal injection). Both protein and mRNA levels of cytokines, such as tumor necrosis factor alpha, interleukin-1β, and interleukin-6 in liver tissues were evaluated by ELISA assay and quantitative PCR. Cyclooxygenase-2, inducible nitric oxide synthase, and nuclear factor-kB protein levels in liver tissues were analyzed by western blotting. Results: Liver injury induced by LPS significantly increased necrosis factor alpha, interleukin-1β, interleukin-6, cyclooxygenase-2, inducible nitric oxide synthase, and nuclear factor-κB in liver tissues. Treatment with ethanol extracts of S. baicalensis prevented all of these observed changes associated with LPS-induced injury in liver mice. Conclusions: Our study showed that S. baicalensis is potentially protective against LPSinduced liver injury in mice.