1.Some opinions of 50 patients with the scapular periarthritis treated by massage therapy and acupuncture point press in Friendship Hospital
Journal of Vietnamese Medicine 2002;269(2):102-104
The scapular periarthritis is a common disease in elderly. 50 patients with scapular periarthritis without the physical causes were treated by the massage therapy and the acupuncture point press. The results have shown that the treatment had good effects in 80% patients.
Massage
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Acupuncture Points
2.Application of microplate almar blue assay for rapid detection of drug resistance of M. tuberculosis strains
Ly Minh Ho ; Hoa Thanh Tran ; Lien Kim Pham ; Hung Van Nguyen ; Phuong Thi Hoang ; Sy Ngoc Dinh
Journal of Preventive Medicine 2008;0(3):60-66
Background: Recently, microplate almar blue assay has been used commonly in detecting gen related to tuberculosis drug resistance, which provides results after 5-7 days with lower cost compared to traditional methods. Objective: To evaluate the application of microplate almar blue assay for rapid detection of drug resistance of M. tuberculosis strains. Subject and Method: A microplate-based assay which uses Alamar blue reagent - an oxidation reduction dye (MABA), was used for the determination of the anti-tuberculosis drug (isoniazid-INH, rifampicine-RIF, streptomycine-STR and ethabuton=EMB) resistance of 96 M. tuberculosis strains isolated from Vietnamese patients in comparison to those obtained by conversional method. Result: MABA showed to have high sensitivity and specificity in testing the sensitivity to individual anti-tuberculosis drugs (from 82.4% for STR to 93.3% for - INH and from 82.5% for EMB to 98.4% for STR; respectively), as well as for the multi-drug resistant M.tuberculosis (86.4% of sensitivity), highly correlated with the result determined by proportion method. Conclusion: MABA reveals the advantage in shortening test time, in simple performance and lower cost compared with the conversional culture based methods.
Microplate almar blue assay
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M. tuberculosis
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drug resistance
3.Isolation and toxicity of Xanthomonas oryzae pv. oryzae in rice and a collection of phages against the pathogen
Ngoc Quynh Anh Pham ; Vo Thanh Phuc ; Pham Do Tra My ; Le Thi My Duyen ; To Hue Ngoc ; Nguyen Minh Thien ; Nguyen Hai Duong ; Le Thi Thuy Tien ; Le Phi Nga ; Hoang Anh Hoang
Malaysian Journal of Microbiology 2024;20(no.1):15-23
Aims:
The objective of the study was to isolate bacteriophages and conduct a comprehensive analysis of their potential against Xanthomonas oryzae pv. oryzae (Xoo) strains in the Mekong Delta, Vietnam.
Methodology and results:
Twelve Xoo strains were isolated from rice fields located in the Mekong Delta, Vietnam. Among these strains, three strains Xoo L019, L020 and L024, showed the highest disease index of bacterial blight. Four
phages specific to Xoo were isolated from soil, water and leaf samples, and their morphologies were determined. In a test against 12 Xoo strains, phage L541, MLA23 or W41 could infect 10 of the 12 Xoo strains, while phage LBH01 could infect 8 of the 12 Xoo strains. The stability of the phages to pH, organic solvents, UV-A and UV-B was also evaluated.
Conclusion, significance and impact of study
The initial characterization of the phages indicates their potential as biocontrol agents against bacterial blight in rice. The study is one of the very first studies about Xoo phages in rice in Vietnam.
4.Immunohistochemical expression in idiopathic inflammatory myopathies at a single center in Vietnam
Dat Quoc NGO ; Si Tri LE ; Khanh Hoang Phuong PHAN ; Thao Thi Phuong DOAN ; Linh Ngoc Khanh NGUYEN ; Minh Hoang DANG ; Thien Thanh LY ; Thu Dang Anh PHAN
Journal of Pathology and Translational Medicine 2024;58(4):174-181
Background:
The identification of idiopathic inflammatory myopathies (IIMs) requires a comprehensive analysis involving clinical manifestations and histological findings. This study aims to provide insights into the histopathological and immunohistochemical aspects of IIMs.
Methods:
This retrospective case series involved 56 patients diagnosed with IIMs at the Department of Pathology, University of Medicine and Pharmacy at Ho Chi Minh City, from 2019 to 2023. The histology and immunohistochemical expression of HLA-ABC, HLA-DR, C5b-9, Mx1/2/3, and p62 were detected.
Results:
We examined six categories of inflammatory myopathy, including immunemediated necrotizing myopathy (58.9%), dermatomyositis (DM; 23.2%), overlap myositis (8.9%), antisynthetase syndrome (5.4%), inclusion body myositis (IBM; 1.8%), and polymyositis (1.8%). The average age of the patients was 49.7 ± 16.1 years, with a female-to-male ratio of 3:1. Inflammatory cell infiltration in the endomysium was present in 62.5% of cases, perifascicular atrophy was found in 17.8%, and fiber necrosis was observed in 42 cases (75.0%). Rimmed vacuoles were present in 100% of cases in the IBM group. Immunohistochemistry showed the following positivity rates: HLA-ABC (89.2%), HLA-DR (19.6%), C5b-9 (57.1%), and Mx1/2/3 (10.7%). Mx1/2/3 expression was high in DM cases. p62 vacuole deposits were noted in the IBM case. The combination of membrane attack complex and major histocompatibility complex I helped detect IIMs in 96% of cases.
Conclusions
The diagnosis of IIMs and their subtypes should be based on clinical features and histopathological characteristics. Immunohistochemistry plays a crucial role in the diagnosis and differentiation of these subgroups.
5.Down-regulated expression of NPM1 in IMS-M2 cell line by (-)-epigallocatechin-3-gallate.
Hoang Thanh CHI ; Bui Thi Kim LY ; Hoang Anh VU ; Yuko SATO ; Phu Chi DUNG ; Phan Thi XINH ;
Asian Pacific Journal of Tropical Biomedicine 2014;4(7):570-574
OBJECTIVETo investigate the inhibited effect of epigallocatechin-3-gallate (EGCG) on the expression of NPM1 in IMS-M2 cells harboring the NPM1 mutations.
METHODSCell proliferation assay was performed to test the effects of EGCG on cell growth of IMS-M2 cells harboring the NPM1 mutations. Western blot analysis were performed to test the protein expression of NPM1, AKT, those associated with apoptosis.
RESULTSEGCG can down-regulate the expression of NPM1 in IMS-M2 cells harboring the NPM1 mutations. Moreover, EGCG also suppressed the cell proliferation and induced apoptosis in IMS-M2 cells.
CONCLUSIONSThe results suggested that EGCG could be considered as a reagent for treatment of AML patients with NPM1 mutations.
6.Assessment of In vitro Antioxidant, Antidiabetic and Cytotoxic Activities of Sphaeranthus africanus Extracts
Tran Thi HUYEN ; Julien DUJARDIN ; Nguyen Thi THU HUONG ; Chung Thi MY DUYEN ; Nguyen Hoang MINH ; Ha Quang THANH ; Dao Tran MONG ; Ly Hai TRIEU ; Nguyen Mai TRUC TIEN ; Mai Thanh CHUNG ; Nguyen Nhat MINH ; Nguyen Thi NGOC DAN ; Huynh LOI
Natural Product Sciences 2023;29(2):98-103
Sphaeranthus africanus is commonly used as a traditional remedy for sore throats and pain treatment in Vietnam. The aerial parts have been studied for its anti-inflammatory and anti-proliferative properties. However, the antioxidant and antidiabetic potential of the plant has not been explored. In this work, hydrophilic extracts of the plant's aerial parts were prepared in order to investigate its antioxidant and anti-diabetic properties. Also, the cytotoxicity of the root was evaluated and compared to that of the aerial parts. All of the extracts inhibited lipid peroxidation with IC 50 values ranging from 2.05 to 3.56 µg/mL, indicating substantial antioxidant activity. At an IC 50 value of 4.80 μg/mL, the 50% ethanol extract exhibited the most potent inhibition of α-glucosidase. The cytotoxic activity of root extracts is 2 to 5-fold less than that of the aerial parts. Nevertheless, dichloromethane and ethyl acetate extracts of the root demonstrated a selective effect on leukemia cells, with no harm towards the normal HEK-293 cell line. This work provides a scientific support for the antioxidant and antidiabetic activity of the plant. Hence, it may find a promising material for the development of novel antioxidant and antidiabetic agents. More research can be conducted on the phytochemistry and anticancer activities of the plant’s root.
7. Down-regulated expression of NPM1 in IMS-M2 cell line by (-)-epigallocatechin-3-gallate
Hoang Thanh CHI ; Phu Chi DUNG ; Phan Thi XINH ; Bui Thi Kim LY ; Hoang Anh VU ; Phan Thi XINH ; Yuko SATO
Asian Pacific Journal of Tropical Biomedicine 2014;4(7):570-574
Objective: To investigate the inhibited effect of epigallocatechin-3-gallate (EGCG) on the expression of NPM1 in IMS-M2 cells harboring the NPM1 mutations. Methods: Cell proliferation assay was performed to test the effects of EGCG on cell growth of IMS-M2 cells harboring the NPM1 mutations. Western blot analysis were performed to test the protein expression of NPM1, AKT, those associated with apoptosis. Results: EGCG can down-regulate the expression of NPM1 in IMS-M2 cells harboring the NPM1 mutations. Moreover, EGCG also suppressed the cell proliferation and induced apoptosis in IMS-M2 cells. Conclusions: The results suggested that EGCG could be considered as a reagent for treatment of AML patients with NPM1 mutations.