Purpose:To investigate the comprehensive treatment value on the malignant Tumor of lung transference.Methods:From January, 1993 to January, 1999, our hospital accepted 75 cases malignant tumor of lung transference in all. It was proved that the primary foci were lungs transference. There are 34 male cases and 41 female cases in the group. The average age is 47 years old the ages(between 27 to 71).Single primary foci reach 12 cases, and multiprimary foci reach 63 cases. Pure operation removals are 4 cases; operation with chemotherapy are 2 cases, and 6 cases are treated with plan radiotherapy before operation with chemotherapy after operation; 17 cases, radio and chemotherapy; 10 cases, only radiotherapy; 36 cases, only chemotherapy. 59 cases in 75 take additional traditional Chinese drugs.Results:The effective rate of nasopharyngeal carcinoma in lung transferring tumor (CR+PR) is 71.4% (20/28) MST is 9.5 month; the effective rate of lung cancer (CR+PR) is 53.3% (8/15) MST 8.5 month; breast cancer, 68.7% (22/32) MST 21 month. The total effective rate of this group (CR+PR) is 66.7% (50/75), CR rate is 36.04%, PR rate is 30.7%, NC rate is 25.3%, PD rate is 8%.Total MST is 13.5 month. Traditional Chinese medicine (TCM) can help the radiotherapy and chemotherapy in the function.Conclusions:The group of cases are for retrospect investigation, there are many disease type, but not many in each type, the analysis of the result shows that they are sensitive to radiotherapy and chemotherapy, the radiotherapy and chemotherapy is a kind of supplementary treatment to low degree malignant tumor of lung transference, it has definite clinical meaning if assisting with traditional Chinese medicine. [

Objective To evaluate the influences of the genotypes,anther developmental stages,and cultural conditions on the efficiency of embryogenic callus induction and plant regeneration in the anthers culture of Bupleurum chinense.Methods The different effects such as four genotypes,plant growth regulators,and temperature condition were compared in the experiments.The histological study was performed with the process of the anther culture.Results The highest inducing rate of embryogenic calli were achieved for the genotypes Zhongcaiyihao(ZCYH),Z4,and Z5 at the early-to middle-uninucleate stages,except for genotype ZPM1 at the tetrad stage.Cold pretreatment increased the production of the embryogenic callus,in which 4-day cold pretreatment improved the production of embryogenic callus from 0% to 2.2% and 5.0% for genotypes ZPM1 and ZCYH,respectively.No embryogenic callus was induced in the medium containing less than 0.75 mg/L 2,4-dichlorophenoxyacetic acid(2,4-D).The highest regeneration rate (34.6%)was obtained in 1/2 MS salts regeneration medium supplemented with 0.1 mg/L 6-benzylmaminopurine (BA).The low concentration of BA was able to promote the embryogenic callus formation and subsequent plantlet regeneration via somatic embryogenesis.Chromosome counting of regenerated plantlets showed mostly diploid plant (2n = 12)with only one haploid plant(n = 6).Because of the low rate of microspore embryo formation,we only tracked the process of embryogenesis from the connective tissue,instead of microspore by histological observations.Conclusion This study establishes an efficient system for embryogenic callus induction and plant regeneration system.This is the first report on the haploid plantlet through the anther culture orB.chinense.

Objective To constructe ,package and identificate the lentiviral vector with overexpression gene Grp78 .Methods We used lentiviral vector and genetic engineering technology to obtain the aim gene fragment and to constructe recombinant plas‐mid .we prepared competent cells and transform the cells .Through positive clone sequencing ,lentivirus was packaged and virus titer was tested .Results Positive cloning sequence comparison results show that the test was passed .Melt curve did not appear mixed peak ,also did not appear abnormal peak broadening .It means that does not appear pollution ,primer dimers and nonspecific amplifi‐cation in the experiments .Conclusion The construction ,packaging and identification of lentiviral vector with over expression gene Grp78 are sucessful .

Purpose: This study was performed to investigate the association of Glasgow prognostic score (GPS), combined positive score (CPS), and clinicopathological characteristics of locally advanced rectal cancer. Methods: Between February 2012 and February 2018, 103 patients with locally advanced rectal cancer treated by neoadjuvant chemoradiotherapy and total mesorectal excision (TME) were retrospectively evaluated. Results: According to the classification of the GPS, 85 (82.5%), 13 (12.6%), and 5 patients (4.9%) were classified as a score of 0, 1, and 2, respectively. Patients were classified into the GPS-low group (GPS of 0, n = 85) and GPS-high group (GPS of 1 or 2, n = 18) with an area under the curve of 0.582 for overall survival (OS). The mean programmed death-ligand 1 (PD-L1) CPS of the whole group was 2.24 (range, 0–70). The PD-L1 CPS of the GPS-high group was higher than the GPS-low group (P < 0.001). Multivariate analysis by Cox proportional hazards model indicated that GPS was associated with OS and diseasefree survival (DFS). Furthermore, PD-L1 CPS was associated with DFS (hazard ratio, 1.050; 95% confidence interval, 1.017– 1.083; P = 0.003). Conclusion Elevated GPS was related to the PD-L1 CPS. GPS and PD-L1 CPS were associated with the prognosis of locally advanced rectal cancer treated with neoadjuvant chemoradiotherapy followed by TME.

OBJECTIVE: To explore the role in the pathogenesis of nasal polyp (NP) by comparison of eosinophil major basic protein (MBP), and neutrophil elastase (NE) in nasal polyps (NP) epithelium, stroma and the secretion of expression. METHOD: Immunohistochemical detection of 30 cases of patients with chronic sinusitis (CRS) NP epithelium NE stroma and the expression and secretion of MBP. RESULT: 1. There were significant differences of the expression of NE and MBP in epithelial tissue, stroma and secretion compared with the control group (P < 0.05); 2. There was not significant difference of the expression of NE and MBP between epithelial tissue and stroma (P > 0.05), while there was significant difference between epithelial tissue and the secretion (P < 0.05); 3. There were significant differences of the average positive expression of MBP and NE among epithelial tissue, stroma and secretion (P < 0.05); 4. MBP and NE were usually degranulated in secretion, while usually located in eosinophils (Eos) and neutrophils (Neu) in epithelial and mesenchymal; 5. There were abundant expression of MBP and NE in epithelial shedding regional, while small amounts of expression in stroma and integrated epithelial; 6. Electron microscopy could show the characteristics of electron density of MBP and NE particles. CONCLUSION MBP and NE collaborated to cause pathological effects on the occurrence of NP.
Adolescent ; Adult ; Aged ; Blood Proteins ; metabolism ; Bodily Secretions ; metabolism ; Eosinophil Major Basic Protein ; Eosinophils ; metabolism ; Female ; Humans ; Leukocyte Count ; Leukocyte Elastase ; metabolism ; Male ; Middle Aged ; Nasal Polyps ; metabolism ; pathology ; Proteoglycans ; metabolism ; Sinusitis ; metabolism ; pathology ; Young Adult