1.Some alterations of hematology and coagulation in burn patients
Journal of Vietnamese Medicine 1998;231(12):49-52
The authors studied on alterations of hematology and coagulation of 2 patient groups, supernatal and deep burn, along 20 first days of intensive treatment. The results showed that decreased platelet aggregation, prolonged bleeding time, reduced prothrombine rate and reduced fibrinogen level in deep burn group, compared to supernatal burn group. These differences were seen mainly at time of T1 and occasional at time T2 and T3.
burns
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Hydrogen-Ion Concentration
2.Anti-inflammatory Triterpenes and Glyceryl Glycosides from Kandelia candel (L.) Druce.
Le Duc DAT ; Nguyen Phuong THAO ; Bui Huu TAI ; Bui Thi Thuy LUYEN ; Seo Young YANG ; Sohyun KIM ; Jung Eun KOO ; Young Sang KOH ; Nguyen The CUONG ; Nguyen Hoai NAM ; Nguyen Van THANH ; Phan Van KIEM ; Chau Van MINH ; Young Ho KIM
Natural Product Sciences 2015;21(3):150-154
Phytochemical investigation of Kandelia candel resulted in the isolation of six triterpenes (1 - 5) and two glyceryl glycosides (6 and 7) and their structures were determined by comparing the spectroscopic data with those of reported values. In present study, we described the inhibitory effects of fractions and isolated compounds from K. candel on pro-inflammatory cytokines (IL-12 p40, IL-6, and TNF-alpha) production in lipopolysaccharide (LPS) stimulated bone marrow-derived dendritic cells (BMDCs). Results indicated that compounds 3, 6, and 7 showed potent inhibition on IL-6 production (IC50 values at less than 0.5 microM, respectively). Meanwhile, compounds 6 and 7 exhibited strong inhibitory effects on the production of TNF-alpha (IC50 values of 1.7 +/- 0.1 and 5.5 +/- 0.2 microM). Compounds 1 and 3 were also showed the inhibitory effects on IL-12 p40 production (IC50 values of 8.9 +/- 0.4 and 3.3 +/- 0.1 microM, respectively).
Cytokines
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Dendritic Cells
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Glycosides*
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Interleukin-12
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Interleukin-6
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Rhizophoraceae*
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Triterpenes*
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Tumor Necrosis Factor-alpha
3.Production, purification and characterization of novel protease from Bacillus amyloliquefaciens D19 isolated in Vietnam
Tan Viet Pham ; Hanh Thi Dieu Nguyen ; Thi Luyen Bui ; Ngoc An Nguyen
Malaysian Journal of Microbiology 2024;20(no.1):85-93
Aims:
Microorganisms play a vital role in the breakdown of natural organic compounds and are valuable objects for worldwide enzyme production. The aim of this study was to identify favorable production conditions for Bacillus amyloliquefaciens D19 protease, followed by the purification and chemical characterization of this novel enzyme to assess its potential applications in various fields.
Methodology and results:
In this study, favorable conditions of protease production from B. amyloliquefaciens D19 were determined using a medium containing soluble starch (1.5%), earthworm extract (1.0%), yeast extract (0.5%), NaCl
(1.0%), at pH 7.0-8.0, 37 °C for 36 h with 150 rpm shaking condition. The protease was purified and had a molecular weight of about 23 kDa. The optimum condition for casein hydrolysis was at 40 °C and pH 6.5-7.0 in the presence of 1.0 mM Na+ or 5.0 mM Zn2+. The enzymatic activity was maintained at 75-100% at 30-50 °C and in pH 6.0-10.0. The values of Vmax and KM were also determined as 1547 U/mg and 6.33 mg/mL, respectively.
Conclusion, significance and impact of study
The identified optimal conditions will serve as the foundation for the production of the 23 kDa B. amyloliquefaciens D19 protease, one of the smallest proteases within the Bacillus genus. Moreover, its notable heat resistance, broad pH tolerance, high substrate catalysis and moderate substrate binding affinity make this enzyme a promising candidate for various applications in the food-feed and brewing industries.
4.Chemicals from Cimicifuga dahurica and Their Inhibitory Effects on Pro-inflammatory Cytokine Production by LPS-stimulated Bone Marrow-derived Dendritic Cells
Nguyen Phuong THAO ; Young Suk LEE ; Bui Thi Thuy LUYEN ; Ha Van OANH ; Irshad ALI ; Madeeha AROOJ ; Young Sang KOH ; Seo Young YANG ; Young Ho KIM
Natural Product Sciences 2018;24(3):194-198
Inflammation is a biological response caused by overactivation of the immune system and is controlled by immune cells via a variety of cytokines. The overproduction of pro-inflammatory cytokines enhances abnormal host immunity, resulting in diseases such as rheumatoid arthritis, cardiovascular disease, Alzheimer's disease, and cancer. Inhibiting the production of pro-inflammatory cytokines such as interleukin (IL)-12p40, IL-6, and tumor necrosis factor (TNF)-α might be one way to treat these conditions. Here, we investigated the anti-inflammatory activity of compounds isolated from Cimicifuga dahurica (Turcz.) Maxim., which is traditionally used as an antipyretic and analgesic in Korea. In primary cell culture assays, 12 compounds were found to inhibit the production of pro-inflammatory cytokines (IL-12p40, IL-6, and TNF-α) in vitro in bone marrow-derived dendritic cells stimulated with LPS.
Alzheimer Disease
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Arthritis, Rheumatoid
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Cardiovascular Diseases
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Cimicifuga
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Cytokines
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Dendritic Cells
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Immune System
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In Vitro Techniques
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Inflammation
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Interleukin-6
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Interleukins
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Korea
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Primary Cell Culture
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Ranunculaceae
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Tumor Necrosis Factor-alpha