Objective To investigate the protective effect of high-density lipoprotein (HDL) on the mice cardiac myocytes induced by oxygen and glucose deprivation (OGD).Methods Cardiac cells of primary scavenger receptor-B1 knockout mice (SR-B1-/-) and normal C57 mice (SR-B1+/+) were obtained by protease digestion and differential adhesion method. ① The two kinds of cells were divided into normal control group (Con group), OGD group, OGD+HDL group. Propidium iodide (PI) staining were used to determine the necrosis of cardiac myocytes. ② SR-B1+/+cardiac cells were divided into Con group, OGD group, OGD+HDL group, and phosphatidylinositol 3 kinase/protein kinase B (PI3K/Akt) inhibitor LY294002 group. PI staining were used to determine the necrosis of cardiac myocytes. TUNEL staining was used to determine the cell apoptosis. The kit was used to determine the contents of MB isoenzyme of creatine kinase (CK-MB) and lactate dehydrogenase (LDH) in the culture medium supernatant. The expressions of SR-B1 and Akt protein were determined by Western Blot.Results ① In SR-B1+/+ cardiomyocytes, HDL could inhibit cell necrosis induced by OGD. There was no protective effect of HDL on OGD in the SR-B1-/- cardiomyocytes.② The study of SR-B1+/+ cells was showed that compared with Con group, necrotic cells were significantly increased and cell activity were significantly decreased, the cell viability were significantly decreased, the contents of LDH and CK-MB in supernatant were significantly increased, the expressions of phosphorylated Akt (p-Akt) and SR-B1 were significantly decreased in OGD group. Compared with OGD group, the number of necrotic cells in the OGD+HDL group was significantly decreased [PI positive cells rate: (26.71±5.94)％ vs. (64.24±18.34)％], the cell activity was significantly increased [(63.84±6.95)％ vs. (26.71±5.13)％], the contents of LDH and CK-MB in supernatant were significantly decreased [LDH (U/L): 896.3±161.5 vs. 1568.3±243.5, CK-MB (U/L): 304.3±72.9 vs. 583.6±81.6], the expressions of p-Akt and SR-B1 were significantly increased (p-Akt/t-Akt: 0.84±0.13 vs. 0.18±0.06, SR-B1/β-actin: 1.23±0.19 vs. 0.09±0.02), with statistically significant differences (allP < 0.05). Compared with OGD+HDL group, necrotic cells in LY294002 group were increased, cell activity was decreased, LDH and CK-MB contents in supernatant were increased, p-Akt and SR-B1 expressions were decreased; there was no statistical difference between LY294002 group and OGD group. There was no significant difference in cell apoptosis among the 4 groups.Conclusions HDL has protective effect on the mice myocardial cells. The mechanism may be related with the up regulation of the expression of SR-B1 protein by the activation of PI3K/Akt pathway.

Objective To survey the current situation of hypertension among "three minority ethnic groups" in Inner Mongolia Autonomous Region.Methods Hypertension epidemiological survey among Three Minority Ethnic Groups and Han nationality aged ≥ 18 years was performed from June to December 2010.Results The prevalence rate of hypertension among the surveyed population was 29.4％ (630/2 146) (standardized prevalence was 34.6％).The prevalence rate of hypertension in male was 33.8％ (359/1 062) (standardized prevalence was 39.9％),and the prevalence rate in female was 25.0％ (271/1 084) (standardized prevalence was 26.6％),and the prevalence rate in male was higher than in female (P ＜ 0.01).The prevalence rate of hypertension for the Oroqen nationality,Ewenki nationality and Daur nationality was 33.8％ (92/272),32.4％ (170/524),and 30.2％ (174/576) respectively (standardized prevalence was 33.7％,33.1％,and 3 1.3％),and which was significantly higher than in the Han nationality (25.1％ (194/774),P ＜ 0.01) (standardized prevalence was 25.8％).The awareness,treatment and control rate of hypertension in the Oroqen nationality residents was 64.1％ (59/92),56.5％ (52/92),27.2％ (25/92) (standardized rate was 63.5％,56.8％ and 27.4％),and 60.0％ (102/170),53.5％ (91/170),24.1％ (41/170) (standardized rate was 62.9％,56.7％,26.6％) in the Ewenki nationality residents,and 59.2％ (103/174),54.0％ (94/174),20.7％ (36/174) (standardized rate was 60.3％,54.7％,21.4％) in the Daur nationality residents,and 65.0％ (126/194),57.7％ (112/194),27.3％ (53/194) (standardized rate was 63.3％,56.5％,27.1％) in the Han nationality residents.Awareness rate,treatment rate and control rate were similar among different nationalities (all P ＞ 0.05).Conclusion The prevalence rate of hypertension among " three minority ethnic groups" residents in Inner Mongolia Autonomous Region is high and comprehensive prevention and therapy strategies are warranted to reduce the hypertension burden in these residents.