Purpose:To evaluate the efficacy and toxic and side effects of brain metastases of lung cancer treated with chemotherapy and conventional radiotherapy. Methods:45 cases of brain metastases of lung cancer were treated with sequential chemotherapy of VM26DDP regimens and conventional radiotherapy. The complete remission and partial remission rate ,1-,2-,3-year survival rate and toxic and side effects were observed.Results:The complete remission and partial remission rate of salitary as well as multiple brain metastases of non-small-cell lung cancer and small-cell lung cancer were (92.86%) and 80.94%,75% and 83.33%, respectively . The 1-,2-,3-year survival rate of salitary and multiple brain metastases of non-small-cell lung cancer was 13.33% and 14.29%、9.5% and 4.76%、9.5% and 0. The 1-,2-,3-year survival rate of salitary and multiple brain metastases of small-cell lung cancer were 50% and 50%、25% and 33.33%、25% and 16.67%, respectively. The Ⅰ,Ⅱ,Ⅲ and Ⅳ degree myelosuppression was 33.33%,28.89%,17.78%,8.89%, respectively.Conclusions:The efficacy of brain metastases of lung cancer treated with sequential chemotherapy by VM26+DDP regimens and conventional radiotherapy is better and with less toxic and side effects.

Objective To investigate the relationship between axillary lymph node status and molecular pathological features in breast cancer and its clinical significance. Methods A retrospective analysis was performed to study the relationship of axillary lymph node metastasis (LNM), positive lymph node (PLN), and lymph node ratio (LNR) with clinical and molecular pathology features in 300 patients with breast cancer. Results Of 300 cases of breast cancer, the positive rate of axillary LNM was 31.0% (13/42) in the Luminal A subtype of breast cancer, yielding a statistically significant difference (P < 0.05), compared with that of other molecular subtypes. Of 144 patients with positive axillary LNM , there were no significant difference of LNR and PLN between different molecular subtypes of breast cancer (P > 0.05). PLN and LNR were all negatively correlated with E-cadherin expression in breast cancer (P < 0.01 and P < 0.05, respectively), correlated with tumor size and TNM staging (P < 0.05), but not with age, menopausal status, and histological grade (P > 0.05). Conclusion The combined phenotype expression of ER, PR, HER-2, and Ki67 (molecular subtypes) are associated with the incidence of axillary lymph node metastases , and E-cadherin expression deficiency and tumor size growth promote the progression of lymph node metastases in breast cancer.

For wild natural medicine, unanticipated biodiversity as species or varieties with similar morphological characteristics and sympatric distribution may co-exist in a single batch of medical materials, which affects the efficacy and safety of clinical medication. DNA barcoding as an effective species identification tool is limited by its low sample throughput nature. In this study, combining DNA mini-barcode, DNA metabarcoding and species delimitation method, a novel biological sources consistency evaluation strategy was proposed, and high level of interspecific and intraspecific variations were observed and validated among 5376 Amynthas samples from 19 sampling points regarded as "Guang Dilong" and 25 batches of proprietary Chinese medicines. Besides Amynthas aspergillum as the authentic source, 8 other Molecular Operational Taxonomic Units (MOTUs) were elucidated. Significantly, even the subgroups within A. aspergillum revealed here differ significantly on chemical compositions and biological activity. Fortunately, this biodiversity could be controlled when the collection was limited to designated areas, as proved by 2796 "decoction pieces" samples. This batch biological identification method should be introduced as a novel concept regarding natural medicine quality control, and to offer guidelines for in-situ conservation and breeding bases construction of wild natural medicine.