1.Establishment of HPLC Fingerprints and Content Determination of 7 Components of Jingu Tongxiao Pill
Shi SUN ; Yifang WANG ; Xinjie ZHAO ; Zhao WANG ; Li ZHANG ; Dandan WANG ; Ailing WU ; Xiaolong WU
China Pharmacy 2021;32(10):1235-1240
OBJECTIVE:To establish the fingerprint of Jingu tongxiao pill ,and to determine the contents of 7 components. METHODS:HPLC method was adopted. The determination was performed on Cosmosil 5C18-MS-Ⅱ column with acetonitrile- 0.02% phosphoric acid solution as mobile phase (gradient elution )at the flow rate of 1.0 mL/min. The detection wavelength was 230 nm and the column temperature was set at 25 ℃. The sample size was 10 μL. HPLC fingerprint of 10 batches of Jingu tongxiao pill was established by using Similarity Evaluation System of TCM Chromatographic Fingerprint (2012 edition),and common peak was identified by comparing with the mixed reference substance. The contents of corresponding components of the identified common peak were determined by the same HPLC method. RESULTS :There were 20 common peaks in HPLC fingerprint of 10 batches of samples ,and the similarity with the control fingerprint was not less than 0.980. By comparing with the mixed reference substance, 7 components were identified , which were loganic acid ,gentiopicroside,paeoniflorin,salvianolic acid B , cryptotanshinone,tanshinone Ⅰ and tanshinone Ⅱ A. The linear range of the above 7 components were 4.509-45.090, 15.090-150.900,14.985-149.850,14.982-149.820,2.967-29.670,1.944-19.440,3.094-30.940 μg/mL(all r>0.999),respectively. The limits of detection were 0.060 1,0.161 0,0.399 6,0.159 8,0.031 6,0.051 8,0.082 5 μg/mL,respectively. The limits of quantitation were 0.200 4,0.503 0,0.999 0,0.399 5,0.079 1,0.259 2,0.412 6 μg/mL,respectively. RSDs of precision ,stability (24 h) and reproducibility tests were all lower than 3.0% (n=6). Average recoveries were 98.81% -100.28% ,RSDs were 0.20%-1.21%(n=6). In 10 batches of samples ,the contents of the above 7 components were 0.441 0-0.969 4,3.283 4-4.733 4, 1.947 7-3.674 9,1.336 6-2.270 9,0.293 2-0.372 1,0.190 2-0.293 9 and 0.352 8-0.518 8 mg/g,respectively. CONCLUSIONS :In this study,HPLC fingerprint and content determination method of Jingu tongxiao pill are successfully established and can be used for quality control.
2.Functional Autapses Form in Striatal Parvalbumin Interneurons but not Medium Spiny Projection Neurons.
Xuan WANG ; Zhenfeng SHU ; Quansheng HE ; Xiaowen ZHANG ; Luozheng LI ; Xiaoxue ZHANG ; Liang LI ; Yujie XIAO ; Bo PENG ; Feifan GUO ; Da-Hui WANG ; Yousheng SHU
Neuroscience Bulletin 2023;39(4):576-588
Autapses selectively form in specific cell types in many brain regions. Previous studies have also found putative autapses in principal spiny projection neurons (SPNs) in the striatum. However, it remains unclear whether these neurons indeed form physiologically functional autapses. We applied whole-cell recording in striatal slices and identified autaptic cells by the occurrence of prolonged asynchronous release (AR) of neurotransmitters after bursts of high-frequency action potentials (APs). Surprisingly, we found no autaptic AR in SPNs, even in the presence of Sr2+. However, robust autaptic AR was recorded in parvalbumin (PV)-expressing neurons. The autaptic responses were mediated by GABAA receptors and their strength was dependent on AP frequency and number. Further computer simulations suggest that autapses regulate spiking activity in PV cells by providing self-inhibition and thus shape network oscillations. Together, our results indicate that PV neurons, but not SPNs, form functional autapses, which may play important roles in striatal functions.
Parvalbumins/metabolism*
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Corpus Striatum/metabolism*
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Interneurons/physiology*
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Neurons/metabolism*
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Neostriatum