In this study,we observed that PGE2 3?10-7~3?10-6 mol/L & nifedipine 1 ?10-5mol/L inhibited lymphocyte proliferation markedly.The effect of nifedipine was completely reversible, however the effects of PGE2(3 ?10-6mol/L)was only partly reversible .In combination of PGE2(3?10-8mol/L) with nifedipine (1 ?10-6mol/L) ,the effect was additive,no significant antagonism and synergism were observed .PGE26? 10-6mol/L and nifedipine 1?10-3 mol/L directly inhibited maerbphage migration but no significant effect on PHA-induced MIF .Preincuba-tation with nefidipine did not influonce macrophage phagocytosis, but PGE2 slightly enhanced it. The results stated above strongly suggests that PGE2 may regulate cells interaction and Ca2+ is required during lymphocyte proliferation.

The leakage of glutamic-pyruvic transaminase ( GPT ) in the supernatant of primary cultures of rat hepatocytes has been used to evaluate the toxicity of the classical hepatotoxin carbon tetrachloride ( CC14 ) . The response of the isolated hepatocyte's preparation to incubate with CCl4 showed a clear dose dependency from 5 to 20 ml/ L in this system and the toxicity increased -with time of incubation. Glycyrrhizin ( 100mg/L ) and sodium caffeic acid 10 to 100 mg/L remarkably reduced GPT release in the medium of hepatocytes with CC14 while Bupleurum kunmingense polysaccharides had no significant effect at the same condition. Glycyrrhizin and caffeic acid might have protective effects against CC14. Further study is necessary. These findings also indicated that the primary cultures of rat hepatocytes is a suitable system for evaluating liver protective drugs as well as studying the function of cell metabolism.