Objective To study the incidence and treatment measures of complications in esophageal stenting.Methods The processes of implantation of esophageal stents in 60 cases were done,of them,59 cases were malignant disease and 1 case was benign stricture.All cases were followed-up for 3~24 months.The types of stents to be used including domestic metal-coated stainless steel Z-stent in 12 cases,domestic gridding-shape nickel-titanium memory alloy coated stent in 47 cases and imports stent in one.Results The processes were successful in all cases,the complications such as arrhythmia in 2 cases,suffocation in one case during operation,and chest discomfort and feeling of foreign body in 49 cases,nausea and vomiting in 15 cases,chronic infection of esophageal stent upper port in 2 cases,stent displacement in 6 cases,stent obstruction in 16 cases,massive hemorrhage,dyspnea and esophago-thoracic fistula in 1 case respectively for 1~36 weeks after operation.After clinical treatment,except 1 patient died due to bleeding shock,all complications were controlled or improved.Conclusion The majority of complications during or after treatment of esophageal stricture with stent implantation are slight,but the indications of operation should be strictly controlled.

Objective To study the application of P1 adhesin protein epitopes in diagnosis of Mycoplasma pneumoniae(Mp) infected patient. Methods The major epitope(P1-534) of P1 adhesin protein were predicted by ProPred and ANTIGENIC according to its primary structure. The high value fragment was cloned into a constructed recombinant vector. The gene was induced to express fusion protein in E. coli host strain BL21(DE3) and the fusion protein was identified by Western blot. BALB/c mice were immunized with purified protein to test its immunogenicity. Then the purified protein was used as antigen to test the serum of Mp infected patient by ELISA, and compared with the Mp whole cell antigen. Results The P1-534 protein was successfully expressed and purified. ELISA data showed that P1-534 protein could elicit high levels of IgG in immunized mice, the sensitivity and specificity of P1-534 were determined to be 85.00% and 97.67%, while the Mp whole cell antigen were 72.50% and 74.42%. Conclusion The results conformed that the recombinant epitope has certain immunogenicity,and its sensitivity and specificity are better than Mp whole cell antigen. P1-534 protein can be used as an antigen for immunodiagnosis of Mp infection.

pneumococcal infections for children. S. pneumoniae was sensitive to penicillin, which was still the first choice of treatment for S. pneumoniae infections. All the isolates were resistant to erythromycin, and ermB was the dominant mechanism of macrolide-resistance.

Objective To investigate the evaluation value of magnetic resonance spectroscopy (MRS) combined with S-100B protein in the severity and prognosis in patients with acute subdural hematoma ( ASDH).Methods Eighty cases of ASDH patients and 20 cases of healthy check-up were selected.MRS was used to test NAA/Cr, Cho/Cr, NAA/Cho, and Glx /Cr in thalamus and corpus callosum.The blood S-100B protein was detected in 72 h after injury.The relationships of those MRS detection indices with glasgow coma scale ( GCS) and glasgow prognostic score ( GOS) for 2 months after injury were analyzed .Results MRS detection in-dex and the S100B protein in ASDH were compared between each group relative to normal control group , all difference had statistical significance ( P <0.05).As aggravating the severity of traumatic brain injury , Cho/Cr, Glx/Cr ratio, and S-100B protein concentra-tion were elevated , and NAA/Cho and NAA/Cr were reduced .All differences were statistically significant among poor recovery , good recovery, and normal control groups ( P <0.05).For patients with traumatic brain injury, there were worse prognosis, the higher ra-tios of Cho/Cr and Glx/Cr, higher concentration of S-100B protein, and lower ratios of NAA/Cho and of NAA/Cr.GCS score and GOS scores were negatively correlated with Cho/Cr and Glx/Cr ratios of corpus callosum , and were positively correlated with NAA/Cho, NAA/Cr ratios of corpus callosum .S-100B protein was positively correlated with Cho/Cr and Glx/Cr, and was negatively correlated with NAA/Cho and NAA/Cr.MRS combined S-100B can improve the prognosis of patients with up to the accuracy of 81%.Conclu-sions MRS detection in the early stage after injury of ASDH patients has important value in assessment of the severity of the injury and its prognosis , the accuracy of assessment of prognosis is improved with a combination of MRS detection and blood S -100 B protein meas-urement.

Objective To explore the value of neuroendoscopy combined with Endport for the surgery of hypertensive intracerebral hemorrhage.Methods We retrospectively analyzed 92 cases of hypertensive intracerebral hemorrhage in our department from January 2016 to February 2018.According to the different surgical methods,they were divided into small bone window group and neuroendoscopic group,47 cases in small bone window group and 45 cases in neuroendoscope group.The amount of intraoperative bleeding,operative time,postoperative hematoma clearance,postoperative rebleeding,hospitalization time,postoperative complications and Glasgow prognosis expansion score (GOS-E) were recorded for statistical analysis.Results Compared with neuroendoscopy group,small bone window group had more bleeding loss [(182.6 ± 34.5) ml vs (103.3 ± 25.7) ml] and longer operation time [(168.7 ± 26.3) min vs (115.7 ± 18.7)min],with significant statistically difference (P ＜ 0.05).The hematoma clearance rate (90.3 ± 5.3) ％ in the small bone window group,was lower than that in the neuroendoscopic group (92.8 ± 6.8) ％,but with no statistical significance (P ＞ 0.05);Postoperative rebleeding occured in 3 cases (6.4％) in small bone window group and 2 cases (4.4％) in the neuroendoscopic group,with no statistically significant difference between the two groups (P ＞ 0.05).Compared with neuroendoscopy group,small bone window group had longer hospitalization time [(18.5 ± 4.3) days vs (13.5 ± 3.8) days],higher tracheotomy rate [15 (31.9％) vs 8 (17.8％)],with significant statistically difference (P ＜ 0.05).The number of patients with GOS-E score ＞ 4 in small bone window group 2 months after operation was less than that in neuroendoscopy group [42.6％ (20/47) vs 62.2％ (28/45)],with significant statistically difference (P ＜ 0.05).Conclusions Endoscopic treatment of intracerebral hemorrhage has the advantages of minimally invasive,short operation time,less intraoperative hemorrhage,low incidence of complications and fast recovery of postoperative function.

Benzene, a recognized hematotoxicant and carcinogen, can damage the human immune system. We studied the association between single nucleotide polymorphisms (SNPs) in genes involved in innate immunity and benzene hematotoxicity in a cross-sectional study of workers exposed to benzene (250 workers and 140 controls). A total of 1,236 tag SNPs in 149 gene regions of six pathways were included in the analysis. Six gene regions were significant for their association with white blood cell (WBC) counts (MBP, VCAM1, ALOX5, MPO, RAC2, and CRP) based on gene-region (P < 0.05) and SNP analyses (FDR < 0.05). VCAM1 rs3176867, ALOX5 rs7099684, and MPO rs2071409 were the three most significant SNPs. They showed similar effects on WBC subtypes, especially granulocytes, lymphocytes, and monocytes. A 3-SNP block in ALOXE3 (rs7215658, rs9892383, and rs3027208) showed a global association (omnibus P = 0.0008) with WBCs even though the three SNPs were not significant individually. Our study suggests that polymorphisms in innate immunity genes may play a role in benzene-induced hematotoxicity; however, independent replication is necessary.
Adult ; Arachidonate 5-Lipoxygenase/genetics/*metabolism ; Benzene/toxicity ; Cell Count ; Cross-Sectional Studies ; Female ; Genetic Association Studies ; Genetic Predisposition to Disease ; Hematologic Diseases/chemically induced/genetics/*metabolism/pathology ; Humans ; Immunity, Innate/genetics ; Leukocytes/*drug effects/metabolism/pathology ; Male ; Occupational Exposure/adverse effects ; Peroxidase/genetics/*metabolism ; Polymorphism, Single Nucleotide ; Vascular Cell Adhesion Molecule-1/genetics/*metabolism