1.Relationship between vitamin D and cardiac autonomic dysfunction
Cheng WANG ; Xuemei LUO ; Jiemin LI
Chinese Journal of Applied Clinical Pediatrics 2015;30(1):6-9
The automatic nervous system(ANS) has 2 main branches:the sympathetic nervous system and the parasympathetic nervous system.The ANS controls mainly automatic bodily functions that are engaged in homeostasis.Autonomic dysfunction lead to many diseases,for example,orthostatic intolerance etc.The relationship between vitamin D and cardiovascular disease has becomes the focus of study gradually in recent years.1,25-dihydroxy vitamin D participates in the regulation of renin-angiotensin axis,vascular effects.Vitamin D deficiency triggers secondary hyperparathyroidism,promotes the development of hypertension,diabetes,dyslipidemia which can influence the incidence and prognosis of cardiovascular disease as well.The study confirmed that vitamin D deficiency is one of the risk factors of cardiac autonomic dysfunction diseases such as orthostatic intolerance.The mechanism is still not very clear.Supplement of vitamin D can offer an effective method to decrease cardiovascular disease risk in populations with low vitamin D status.
2.Bedside ultrasonographic diagnosis in children with superior vena cava syndrome after cardiac operations
Yongqing LI ; Yi LUO ; Pei CHENG ;
Chinese Journal of Ultrasonography 1993;0(04):-
Objective To explore the findings and clinical value of bedside ultrasonography in superior vena cava(SVC) syndrome in children after cardiac operations for congenital heart disease. Methods Bedside ultrasonography was performed on 6 children suspected of SVC syndrome after 5-38 hours[(22?11) hours] postoperatively,to view the structure and blood flow of SVC and its main branches. All the patients were reoperated as soon as diagnosed. Results Five children got severe SVC obstruction, marked with stenosis, turbulent, continuous and high velocity in SVC. Color Doppler flow imaging presented almost absence of flow due to nearly complete occlusion of the innominate vein in 1 child. Ultrasonographic diagnosis was confirmed by the reoperative findings. All the patients were cured by reoperation and discharged with normal ultrasonographic results. Conclusions Bedside ultrasonography can provide early and accurate diagnosis in children with SVC syndrome after cardiac operation.
3.Quantitative analysis of IGF1Rs on human hepatocellular HepG_2 cells
Chaoxue LUO ; Shaojun CHENG ; Qianwei LI
Journal of Third Military Medical University 1983;0(03):-
Objective To quantitatively analyze type 1 receptor of insulin-like growth factors (IGF1Rs) on the HepG 2 cells. Methods 1H7 (monoclonal antibody against IGF1R) was labeled with radionuclide 131 I by Iodogen method. The mixture was purified with Sephadex G-50 column, 131 I-1H7, and its unlabeled counterpart 1H7 competitively bound to IGF1Rs on the HepG 2 cells. The average number of IGF1R on the HepG 2 cells was analyzed by Scatchard method. Results The Bmax of HepG 2 cells (2.0?10 5) in every well was 6.746?10 -12 mol/L. The expression of IGF1R on the HepG 2 cells was 2.03?10 4/cell. Conclusion IGF1Rs are expressed on the HepG 2 cells with high density and high affinity, which may be used in other related studies.
4.Primary Study on the Effect of Xanthotoxin,and Umbelliferone in Photochemotherapy
Jiarong LI ; Nanqiu CHENG ; Chaodong LUO
Chinese Traditional and Herbal Drugs 1994;0(02):-
External use of either xanthotoxin or umbelliferone alone in normal rats showed that the chromosomes of the myelocyte were mutated at an aberrant rate of 2.0% and 0.2%, respectively (P
5.The effect of the recombinant human resistin on lipid metabolism by AMPK pathway in HepG2 cells
Zhaofan LUO ; Fangping LI ; Hua CHENG
The Journal of Practical Medicine 2017;33(11):1743-1747
Objective To investigate the effect of rh-resistin on lipid metabolism in HepG2 cells and to elucidate its relation to AMPK pathway. Methods We treated the HepG2 cells with 50 ng/ml rh-resistin and 0.5 mmol/L palmitic acid,used siRNA technique to inhibite α2 subunite expression of AMPK in HepG2 cells and quantitative RT-PCR to detect ACC1,ACC2,and HL mRNA expression levels of related lipid metabolism genes. The P-AMPK-Thr172 of AMPK and P-ACC-Ser79 of ACC were determined by Western blotting. The Lipid accumu-lation in cells was determined by images of Laser Scanning Confocal Microscope after Nile red staining. Results Rh-resistin decreased the AMPKα2,HL mRNA expressions and the phosphorylation level of AMPK and ACC in both basal and insulin-stimulated conditions (P < 0.05),had no influence on ACC2 mRNA expressions (P >0.05),while it increased ACC2 mRNA expressions and cytoplasmic lipid droplets in the same conditions (P <0.05). Conclusion Rh-resistin may affect lipid metabolism via AMPK pathway with increase of fatty acid synthe-sis and inhibition of triglyceride catabolism,which leading to lipid accumulation in HepG2 cells.
6.Effect of recombinant human resistin on glucose metabolism by AMPK pathway in HepG2 cells
Zhaofan LUO ; Fangping LI ; Hua CHENG
The Journal of Practical Medicine 2016;32(10):1556-1560
Objective To investigate the effect of rh-resistin on glucose metabolism in HepG2 cells and to elucidate whether the underlying mechanisms are related to AMPK pathway. Methods Cells transfected with control siRNA or AMPKα2 siRNA were cultured in 6-well plates and then treated with 50 ng/mL rh-resistin for 24 hours , while untransfected cells were treated with or without 50 ng/mL rh-resistin on the same conditions , followed by serum-starving in glucose-free DMEM for 3 ~ 5 hours in the continued absence or presence of rh-re-sistin. Then the cells were treated with or without insulin for 2 hours. AMPKα2, G6Pase, PEPCK and Glut2 mRNA expression levels were determined by quantitative RT-PCR. The phosphorylation state of AMPK was deter-mined by Western blotting. Glycogen synthesis was measured by the incorporation of D-[U-14C] glucose to glycogen. Results Rh-resistin suppressed the AMPKα2, Glut2 mRNA expressions, and reduced the phosphory-lation level of AMPK and glycogen synthesis on both basal and insulin-stimulated conditions (P < 0.05), while it accelerated G6Pase and PEPCK mRNA expressions on the same conditions (P < 0.05). The mRNA expression levels of G6Pase , PEPCK , Glut2 and the phosphorylation level of AMPK and glycogen synthesis were signifi-cantly different between the rh-resistin group and the rh-resistin in conjunction with AMPKα2 siRNA-treated group. Conclusion Rh-resistin may affect glucose metabolism in HepG2 cell via AMPK pathway.
7.Levels of Serum Leptin and Insulin and Their Relationships with Growth and Development in Preterm Infants
zhi-hua, LUO ; li-li, WANG ; yan, CHENG ; yang, WANG
Journal of Applied Clinical Pediatrics 1986;0(02):-
Objective To explore the levels of serum leptin and insulin and their relationships with growth and development in premature infants.Methods The serum leptin and insulin concentrations of 37 premature infants were examined by the radioimmunoassay(day 1 and day 10,respectively),and anthropometric measurements were performed at birth.The blood sugar and triglyeride,cholesterol were tested at the same time.Twenty full-term newborn without severe diseases were controlled in 24 h after delivery and the level of leptin and insulin of them were detected,too.The results were analyzed by SPSS 10.0 software.Results The leptin and insulin levels in preterm infants were significantly lower than those in full-term infants[(6.45?2.24)?g/L vs(8.56?1.33)?g/L,(5.84?1.07)IU/L vs(10.85?5.24)IU/L Pa0.05).Serum leptin was positive correlation with serum insulin(Pa0.05).Conclusions Serum leptin and insulin can be the markers that reflect intrauterine nutrition status of preterm infants and the growth and development of fetus.A dipoinsular axis may be functional before 34 weeks gestation without maturity.The changes of leptin and insulin concentrations are useful for metabolism and growth in preterm infants.
8.A study on anxiety-depression situation and related factors of 120 puerpera before delivery
Chun-Lian TAO ; Li-Li LUO ; Hai-Cheng LIN ; Lin-Hong LUO ; Li-Ping WU ;
Chinese Journal of Primary Medicine and Pharmacy 2006;0(10):-
Objective To investigate the status of anxiety-depression and the relevant factors in puerpera be- fore delivery.Methods The emotion of anxiety and depression in puerpera before delivery was evaluated by self-rat- ing anxiety scale(SAS)and self-rating depression scale(SDS)which had satisfactory validity and reliability.Results The values of anxiety and depression in puerpera were higher than those of general crowd(P
9.Encephalic lymphomatoid granulomatosis.
Li YU ; Zhen-Xi LIU ; Sha XIAO ; Cheng-Yi LUO
Chinese Journal of Pathology 2005;34(2):121-122
10.The relationship between the hemodynamic changes in head-up tilt test and the height, body quality and body mass index in children
Haiyan LUO ; Ping LIN ; Fang LI ; Cheng WANG
Chinese Pediatric Emergency Medicine 2014;21(8):486-488
Objective To explore the relationship between the hemodynamic changes in head-up tilt test(HUTT) and the height,body quality and body mass index in children.Methods We choosed 1906 cases of unexplained syncope,headache,dizziness in children who came from Children Syncope Outpatient in the Second Xiangya Hospital of Central South University from Jan.2000 to Aug.2012,Male 964 cases and female 942 cases.Their ages were 2 to 17.92 years old.The average age was 10.84 ± 2.97 years old.The height and body mass in children was measured,BMI was calculated.After obtain written informed consent subjects or guardian,the HUTT was carried.According to the result of HUTT and reaction type,the children were divided into HUTT negative group,orthostatic tachycardia syndrome(POTS) group,vasovagal syncope (VVS) vascular inhibitory type group,VVS heart inhibited type group,the VVS mixed group.Results Compared to the HUTT negative group,the age increased in POTS group and VVS vascular inhibitory group and VVS mixed group(P < 0.05),height and body quality and body surface area increased in POTS group and VVS vascular inhibitory group and VVS mixed group(P < 0.01).Compared to POTS group,body quality and body surface area reduced in VVS vascular inhibitory group and VVS mixed group(P < 0.05).VVS heart inhibitory group was no statistically significant difference compared with the other groups (P > 0.05).Conclusion There is a certain relationship between the hemodynamic changes in HUTT and the height,body quality and BMI in children.