Objective To investigate the cleaning quality of frequently touched object surfaces in a hospital,so as to formulate effective evidence-based intervention measures.Methods Frequently touched object surfaces in common wards,operating rooms,and intensive care unit(ICU)of the hospital between October 2014 and February 2015 were labeled by fluorescent adhesive agents 1 -2 times a week,objects were labeled before patients’discharge and at the end of operation,cleaning quality of frequently touched object surfaces were checked after routine cleaning. Results A total of 2 131 object surfaces were monitored,fluorescent adhesive agents on 1 732 objects were cleared, clearance rate was 81 .3%,hygienic clean was qualified.642,650,and 839 objects surfaces in common wards, operating rooms,and ICU were monitored respectively,the clearance rates were 82.1 %,75.8%,and 84.9%respectively,the clearance rates among three wards were significantly different (χ2 =19.964,P <0.001 ).In com-mon wards,fluorescent clearance rates of call buttons,power switches,and equipments were 51 .3%,55.2%,and 58.5% respectively;in operating room,fluorescent clearance rates of room wall,control panel of anesthesia ma-chine,bed-adjusting device,keyboards,and infusion pumps were 38.6%,48.8%,60.0%,61 .3%,and 68.4%,re-spectively;in ICU,fluorescent clearance rates of equipments was 65.9%,all were unqualified.Conclusion The en-vironmental hygiene quality is generally qualified in this hospital,but the cleaning quality of frequently touched ob-ject surfaces needs to be further improved.

Resveratrol is a kind of polyphenolic compounds, and widely exists in plants.It can be provided with comprehensive physiological and pharmacological effects. In this paper, the authors reviewed the effect of resveratrol on myocardial ischemia-reperfusion injury, the effective treatment of atherosclerotic diseases and coronary heart disease, and discuss the protective effect and the mechanism of resveratrol on cardiovascular disease through the effect of the electrophysiology and the accommodation of the blood vessel.

Objective To evaluate the clinical efficacy of laparoscopic surgery in the treatment of endometriosis. Methods Clinical data of 102 patients with endometriosis treated by laparoscopic surgery were retrospectively analyzed. Results There were no conversions to open surgery or complications in all the patients. The operation time was 80 5 min ? 28 3 min, the intraoperative blood loss was 25 2 ml ? 23 2 ml and the postoperative hospital stay 3 0 d ? 1 2 d. Follow-up for 6 months ～ 66 months in 83 patients showed 21 cases of recurrence. Normal pregnancy was seen in 23 patients among 53 patients with endometriosis associated with infertility. Conclusions Laparoscopic surgery is effective for endometriosis, but the danger of recurrence should not be overlooked.

The control meaurees and endemic situation were analysed in Dali Prefecture,Yunnan Province. The infection rates of population and livestock decreased from 6.42% and 3. 81% in 2004 to 0.07% and 0.15% in 2008, respectively. No infected snail and acute schistosomiasis case were discovered in 2008. The proportion of villages with serious endemic situation decreased from 49.42% in 2004 to 7.51% in 2008, and 92.48% of villages reached the criteria of transmission control or transmission interruption. The endemic situation and snail status of Dali Prefecture have decreased significantly after the implementation of the comprehensive control project, but the consolidation and enlargement of the control result still remains a challenge.

Objective To provide an approach to research of peroxisome proliferator activated receptor (PPAR) ? 2 function, mouse PPAR? 2 (mPPAR? 2) gene was cloned and its transient expression in eukaryotic cells was carried out. Methods mPPAR? 2 mRNA from epididymis fat pad of Chinese Kunming mice was amplified by RT PCR and subcloned into plasmid pcDNA3 to generate the recombinant plasmid pcDNA3/mPPAR? 2 which was confirmed to contain the amplified target gene segments with fluorescence sequencing. The recombinant plasmid pcDNA3/mPPAR? 2 was used to transfect COS 7 with lipofectamine and the expressing product was detected with immune fluorescence assay and Western blot. Results The sequencing results for amplified target gene showed that the sequence of mPPAR? 2 from epididymis fat pad of Chinese Kunming mice is similar to that of mouse PPAR? 2 in Genbank, only at the site of 383 amino acid where Ser (AGC) substitutes Asn (AAC). pcDNA3/mPPAR? 2 was efficiently expressed in eukaryotic cells in vitro. Conclusion This work is the experimental basis for further researching on PPAR? 2 function.

Objective To express the mouse peroxisome proliferator activated receptor ? 2 (mPPAR? 2) in NIH3T3 cells induced by the recombinant retrovirus vector. Methods mPPAR? 2 gene digested from the recombinant plasmid pcDNA3/mPPAR? 2 containing the target gene segment was subcloned into retrovirus vector pGCEN to generate the recombinant retrovirus pGCEN/mPPAR? 2. The recombinant retrovirus pGCEN/mPPAR? 2 and pGCEN were used to transfect PA317 cells with LipofectAMINE, and anti G418 clones of PA317 cells were selected and viral supernatants were harvested and used to infect NIH3T3 cells. The expressing products were identified with immune flurescence assay (IFA) and Western Blot. Results The recombinant retrovirus pGCEN/mPPAR? 2 was constructed, and 5?10 4 CFU/ml and 6?10 5 CFU/ml of pGCEN/mPPAR? 2 containing and pGCEN containing viral supernatants were obtained respectively. mPPAR? 2 was expressed in NIH3T3 cells mediated by the recombinant retrovirus vector. Conclusion This work is the basis for the foundation of adipocyte differentiation model in vitro and further researching on the molecular mechanism of adipocyte differentiation induced by PPAR? 2.

Objective To elucidate the significances of health education in the prevention and treatment of gout and hyperuricemia.Method Health education scientific knowledg was done through approaches of specific proclamation in clinic out patients,screening the directly-related members of patient family,training medical staffs and popular vehicle propaganda etc.Results Health education improved the diet structure and life mode of patients,promoted early diagnosis and treatment of patients.It also had favourness in timely detection of other patients in directly-related members of ones family.Conclusion Health education combined with conventional pharmacological therapy could produce a better efficacy than that of single pharmacological ones.

Objective To compare two assay methods, namely immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH), in the detection of Her-2/neu status in mammary cancer tissues. Methods In 20 samples IHC technique was applied to detect the Her-2 protein in the cytomembrane of mammary cancer, and FISH was used to assess the amplification of Her-2/neu gene, and SPSS 10.0 software was employed to analyze the relationship between the two methods. Results The coincident rate of two methods was 85%, therefore there was a significant positive correlation between the two techniques (?~2=80.00, P=0.00). Conclusion The two assay methods, IHC and FISH showed a good coincidence in the detection of Her-2/neu status in mammary cancer tissues.

OBJECTIVE To explore a management method for the prevention and control of infectious diseases in hospital. METHODS We applied the PDCA (plan,do,check and action) model to establish a management strategy for the prevention and control of infectious diseases for three years. The components of the management model included providing educational program for medical staff,designing and realizing direct network system for infectious disease reporting,monitoring diagnosed infectious diseases ,analyzing their epidemic tendency,guiding medical staff to prevent and control infectious diseases,and strengthening the surveillance,feedback,rewards and punishment. RESULTS The rate of delayed or missed report of infectious diseases was decreased from 5% to 1.1% by monthly check and from 6.8% to 0% by seasonally cross check. Although 46 cases of AIDS,5 cases of caesarean birth with HIV possitive and 1445 cases of hand-foot-mouth disease were admitted in our hospital,No medical staff and other patients was crossly infected. CONCLUSIONS Our way of management based on PDCA model can strengthen the prevention and control of infectious diseases and assure medical quality and patient safety.