Objective To explore the clinical efficacy of theophylline retard-20 tablet in pediatric asthma treatment.Methods From April 2010 to December 2014 in our hospital 136 cases of children with infantile asthma were selected and divided into observation group and control group.Patients in the observation group were treated with sulfate aerosol inhalation therapy,patients in the control group were given oral theophylline retard-20 tablet treatment.After treatment,the treatment results were analyzed.Results In the observation group,functional recovery was found in 37 cases,significant progress in 20 cases,on effect in 11 cases,the total effective rate was 83.82％;Adverse reactions such as pharyngeal discomfort was found in 3 cases,upset stomach in 0 cases,heartburn in 0 cases,the incidence was 4.41％.In the control group,68 functional recovery was found in 19 cases,and remarkable progress in 26 cases,on effect in 23 cases,the total effective rate was 66.18％;Adverse reactions such as pharyngeal discomfort occurred in,4 cases,upset stomach in 3 case,heartburn in 4 cases,the incidence was 14.71％.The effect effective treatment was better than that of control group (P ＜ 0.05),the incidence of adverse reactions in observation group was significantly lower than the control group (P ＜ 0.05).ConcltSior The treatment of infantile asthma with salbutamol sulfate aerosol therapy is very effective and has,clinical significance,is worth further promotion and application.

Objective To investigate the characteristics of rifampin(RFP) and isoniazid(INH) resistance genes of Mycobacteri-um tuberculosis in Ganzhou region .Methods Gene chip technology was employed to detect common resistance genes in 130 sputum samples with smear positive acid-fast staining and Ct values less than 30 .Results Among 130 samples ,34 samples(26 .2% ) were found resistance ,including 30 samples(23 .1% ) resistant to RFP ,26 samples(20 .0% ) resistant to INH ,and 21 samples(16 .2% ) resistant to both .The mainly mutation of RFP resistant gene was rpoB gene 531(C→ T ) ,accounting for 42 .4% ,while the mainly mutation of INH resistant gene was katG gene 315(AGC→ACC) ,accounting for 65 .4% .Conclusion The mutation charateristics of RFP and INH resistance-associated genes of Mycobacterium tuberculosis in Ganzhou region is consistent with domestic and foreign research .

Klebsiella Pneumoniae is an important pathogen for various infections clinically,in which hypervirulent Klebsiella pneumoniae (hvKP) mainly causes pyogenic liver abscess.By now,there are no standardized method to identify hvKP strains.hvKP strains are usually with hypermucoviscous phenotype,and the prevailing serotypes and clonal types are K1,K2 and CC23,CC65,respectively.Genes rmpA,magA,alls and kfu contribute to the pathogenicity of hvKP.This paper reviews the identification,clonal types and serotypes,hypermucoviscous phenotype and some other virulent genes of hvKP.

With the rapid development of clinical laboratory technology and clinical improvement,the discordance between department of microbiology and clinical departments got more and more.Based on the analysis of interface issues existed between them,we discussed and explored how to do a wonderful job to communicate with each other.(Chin J Lab Med,2013,36:375-376)

Objective To study the roles of advanced glycation end products and its receptor on fetal brain injury of gestational diabetes mellitus (GDM) rats.Methods Twenty one adult pregnant Wistar rats were administered streptozotocin (STZ) intraperitoneally to induce GDM rats model.The fourteen pregnant rats were divided into two groups according to the fasting glucose on the 3rd day of pregnancy:severe GDM group with the fasting glucose ＞ 16.7 mmol/L and mild GDM group with the fasting glucose between 6.7 - 16.7 mmol/L Another seven pregnant rats were chosen as the severe GDM and intervention with micronutrient group,receiving gavage with micronutrient during the whole pregnancy.Five control rats received the same volume of citric acid buffer.All the pregnant rats were tested fasting glucose from the tailvein and their weight on the pregnant day 3,13 and 19.Maternal serum levels of AGE were measured by ELISA and RAGE levels in the embryonic brain tissues were tested by immunohistochemistry.Results ( 1 ) There was no statistically significant difference of pre-pregnancy fasting glucose level among all groups (P ＞ 0.05 ).The fasting glucose levels on the 3rd day and the mean fasting glucouse level of pregnancy in the severe GDM group and the severe GDM and intervention with micronutrient group were higher than those of the control group ( P ＜0.05 ).And there was no significant difference between the severe GDM group and the severe GDM and intervention with micronutrient group (P ＞0.05 ).(2)The serum AGE levels in the severe GDM group and the mild GDM group were( 1037 + 38) ng/L and( 880 ± 34) ng/L respectively,with no significant difference ( P ＞ 0.05 ).The serum AGE levels in the control group and the severe GDM and intervention with micronutrient group were (857 ± 32 ) ng/L and (988 ± 37 ) ng/L,and the difference was statistically significant ( P ＜ 0.05 ).The serum AGE levels in the severe GDM and intervention with micronutrient group and in the mild GDM group had no significant difference ( P ＞ 0.05 ).( 3 ) The serum AGE levels in the severe GDM group,mild GDM group and the control group were positively associated with the mean glucose level of pregnancy ( r =0.603,P ＜ 0.05 ) and the grlucose on the 3rd day of pregnancy (r =0.704,P ＜ 0.05 ).(4)The fetal brain nerve cell number and morphology in the control group were normal.While in the mild GDM group fetal brain nerve cells decreased,the proliferation and swelling of glial cells were seen.In the severe GDM group and the severe GDM and intervention with micronutrient group,the fetal brain cells furtherly reduced,and large vacuole around the cells,deformation and debris of the cells were seen. Glial scar formation was visible in some fetal brain tissues.There was a few RAGE expression in the control fetal brain tissues.In the mild GDM group and the severe GDM group,RAGE expression increased significantly.And the RAGE expression intensity in the severe GDM and intervention with micronutrient group was between the severe and the mild GDM groups.Conclusions( 1 ) Abnormal fetal brain development of GDM rats was associated with the increase of maternal serum AGE and the enhancement of RAGE expression in fetal brain tissues,which suggested that AGE/RAGE pathway may play an important role in the fetal brain injury of GDM rats.(2) Micronutrients can reduce the brain damage of GDM fetuses.

Objective To investigate the expression of CD40 protein in bladder urothelium cancer and to explore its correlation with clinical stage, pathological grades, cell apoptosis. Methods Expression of CD4o protein was evaluated by immunohistochemistry in paracancerous tissues (20 cases) and bladder urothelium cancer tissues (78 cases). Apoptosis of bladder urothelium cancer tissues was detected by Hoechst to determine the apoptotic rate (AR). Results The expression rate of CD40 protein was 2/20(10 %) in the cases of paracancerous tissues, 55/78(70.5%) in the cases of bladder urothelium cancer tissues, a statistically significant differences of CD40 staining was observed between paracancerous tissues and bladder urothelium cancer tissues (P ＜0.01). Overexpression of CD40 was strongly inversely related to the tumor grade and clinical stage; the positive group of CD40 protein was (12.60±0.38)%, the negative group of CD40 protein was (6.77±0.53)%, a statistically significant differences of apoptosis were observed between CD40 protein positive group and CD40 protein negative group (P ＜0.01). It showed CD40 protein might induce cell apoptosis. Conclusion Expression of CD40 protein is closely related with clinical stage, pathological grade and cell apoptotic rate and may offer reliable evidence for diagnosis, treatment and prognosis in bladder urothelium cancer.

The bookmade precise discussions on syndrome differentiation and treatment of infantile malnutrition.This article tried to explore the diagnostic and therapeutic features of infantile malnutrition from four aspects:etiopathogenisis and location of disease:classificafion of therapeutical principle;andinternal and external therapy.

Objective To investigate the value of laparoscopy in the diagnosis and treatment of unexplained chronic abdominal pain.Methods From January 2014 to February 2016, clinical data of 56 unexplained chronic abdominal pain undergoing laparoscopic surgery were analyzed retrospectively.Laparoscopic exploration was performed firstly and then the corresponding abnormalities were diagnosed and dealt with accordingly.Results The disease causes were explicit in 48 cases (85.7%), including 25 cases of abdominal adhesions, 8 cases of chronic appendicitis, 2 cases of small intestinal diverticulum, 2 cases of intestinal tuberculosis, 2 cases of lymphoma, 5 cases of endometriosis, 2 cases of tubal adhesions, 1 case of peritoneal metastasis of gastric cancer after surgery, and 1 case of appendiceal mucinous adenocarcinoma.Surgical treatment was performed in 43 cases, 3 of whom were converted to laparotomy.There were no postoperative complications in the 56 cases.The follow-up rate was 92.9% (52/56), and lost of follow-up was in 4 cases.The follow-up duration was from 3 months to 3 years (median, 19.4 months).There was 1 death case (peritoneal carcinomatosis).There were 35 cases (62.5%) of complete relief of abdominal pain (no recurrence of preoperative abdominal pain symptoms) and 12 cases (21.4%) of pain relief (preoperative pain decreased or interval extended).The total effective rate was 83.9% (47/56).Conclusion Laparoscopy is of high value in the diagnosis and treatment of unexplained chronic abdominal pain.

Objective To investigate the clinical characteristics of children with dyspepsia diarrhea and the effect of Clostridium butyricum powder combined with changweikang granules on the treatment of the children with this diseases.MethodsFrom February 2013 to October 2015 in department of pediatrics, Tonglu County the First People's hospital, 76 cases with dyspepsia diarrhea as the research object, were randomly divided into the experimental group and the control group, 38 cases in each group.The two groups were given changweikang granules, at this basis, the observation group were given clostridium butyricum powder.3 days for a course of treatment, the two groups were treated for 2coureses continuously.ResultsBefore treatment, there were no significant differences in clinical symptom scores between the two groups.After treatment, the scores of loss of appetite, abdominal pain, diarrhea, abdominal distension and stool in the experimental group were lower than those in the control group, the difference was statistically significant (P<0.05);Before treatment, the serum motilin, gastrin and somatostatin levels in the two groups were not statistically significant.After treatment, the serum motilin and gastrin levels in the experimental group were lower than those in the control group, and the level of serum somatostatin was higher than that of the control group, the difference was statistically significant;After treatment, the total effective rate in the experimental group was 92.11% higher than that in the control group (73.68%), and the difference was statistically significant (P<0.05).ConclusionPediatric indigestion diarrhea often showed loss of appetite, abdominal pain, diarrhea, abdominal distension and stool.The effect is good which clostridium butyricum powder combined with changweikang granules on the treatment of infantile indigestion diarrhea, it is worthy of clinical application.

Quantum dots( QDs) as a new type of nanostructured luminescent materials have been widely used in biology, materials science, and physical optics. QD-based biosensors can rapidly and accurately detect biological macromolecules or inorganic molecules both in vivo and in vitro. The surface of QDs, prior to their biological detection applications, needed to be modified to enhance their fluorescence properties and lower their biological toxicity. Currently, the surface modification technologies mainly include polymer modification, thiol compounds modification, mercapto propionic acid compounds modification and organic groups modification. Various biosensors can be developed by adopting different modification techniques, which have been adopted to locate and track a variety of biological molecules in vivo. Although a large number of literatures have reported the biological applications of QD-based biosensors, rare systematic reviews of surface modification technologies on QDs have been witnessed. In this paper, we reviewed the surface modification technologies of QDs in biosensors and their medical applications.