Many surface proteins of Gram-positive bacteria are anchored to the cell wall by the action of sortase enzymes, a recently discovered family of cysteine transpeptidases. As the surface proteins of pathogens are frequently required for virulence, the sortase might be a suitable target for the development of anti-Gram-positive drugs. Recently, the mechanism and active sites of sortase was elucidated by the research of sortaseA(SrtA) in Staphylococcus aureus and a series of SrtA inhibitors have been identified for providing the basis for further development of potent inhibitors.

Objective:To investigate the attentional bias to emotional information in college students with optimistic and pessimistic attributional style.Methods:A total of 83 college students (20 males and 23 females in optimistic group,20 males and 20 females in pessimisticgroup) were recruited online.The 2 cue validity (valid,invalid) × 2 emotional type (positive,negative) × 2 attributional style (optimistic group,pessimistic group) hybrid design was used to investigate the attentional bias to emotional information in college students with optimistic and pessimistic attributional style,adopting the cue-target paradigm.Results:Under valid situation,optimistic group had responded to positive (cue) target faster than pessimistic group [(311.4 ± 26.6) ms vs.(324.1 ±± 47.0) ms,P ＜ 0.05],while there was no significant difference in reaction times (RTs) on negative (cue) target between two groups (P ＞ 0.05).Under invalid situation,optimistic group had responded to negative target faster than pessimistic group [(331.0 ±± 31.7) ms vs.(337.4 ± 50.0) ms,P ＜ 0.05],whereas there was no difference in their RTs to responding to positive target(P ＞ 0.05).Conclusion:It suggests that optimistic individuals and pessimistic individuals respectively have selective attentional bias to positive information and negative information.

Objective To investigate the relationship between evidence-based nursing tactics and quality of life of breast cancer patients so as to provide the reference for the best nursing strategy .Methods 87 patients who were diagnosed with breast cancer received surgical treatment were randomly divided into experimental group (44 cases) and control group (43 cases) .Patients in control group were given general nursing ,while those in experimental group were given evidence-based nursing tactics on the ba-sis of general nursing .Both groups were asked to fill in the quality of life questionnaire after 4 months breast cancer treatment and before treatment .Results The quality of life in experimental group was significantly improved after treatment compared with that of before treatment(P< 0 .05) ,the quality of life in the experimental group received evidence-based nursing tactics is significantly better than that of in control group (P< 0 .05) .Conclusion Evidence-based nursing tactics can improve the quality of life in breast cancer patients .

The effect of colored noise on circadian oscillation and internal signal stochastic resonance(ISSR) has been studied in a Neurospora circadian clock system. The result shows that the correlation time of colored noise can affect strongly the strength of ISSR. For the case of no external signal(ES), the correlation time of colored noise plays a suppressive role for ISSR, and the suppressive role is increased with the increment of correlation time of colored noise. When the ES is injected to the system, with the increasing of the correlation time of colored noise, not only its suppressive role for ISSR is increased, but also the single peak ISSR can be transformed as internal single stochastic bi-resonance (ISSBR). There exists an optimal frequency of ES for the ISSR information amplification, while the ISSR is suppressed for another frequency of ES. In contrast to external colored noise, internal colored noise is more efficient to sustain and amplify the ISSR information. Furthermore, there exists a critical noise intensity for destroying the difference between white and colored noises.

OBJECTIVE: To summarize the clinical datas of thepatients with invasive laryngeal fungal infections in, discuss pathogenesis and treatment methods. METHOD: Eleven cases of invasive laryngeal fmycosis who were collected from September 2006 to February 2010 with electronic laryngoscopy, aspirate smear and culture and tissue biopsy for pathological diagnosis, were restrospectively analyzed. Those patients were received iv fluconazole, treatment of Oxygen Atomization of amphotericin B solution and taking itraconazole orally. The hepatic and renal functions of the patients were monitored in the course of treatment. RESULT: All the cases were diagnosed of invasive laryngeal mycosis. 1 patient showed liver dysfunction in the second week during treatment. And continuing the treatment after using liver protection drugs. All symptoms of the patients were improved and no recurrence happened during the 1-6 years of follow-up. CONCLUSION Invasive laryngeal fmycosis was correlated with occupation exposure, abusing of antibiotics and low immunity. Laryngeal mycosis was Diagnosised mainly depended on the pathological examination. The positive rates of the secretion smear was low. The effects of iv fluconazole, Oxygen Atomization of amphotericin B 2-4 weeks, and 4 weeks of taking itraconazole orally were safety and reliable.
Administration, Oral ; Amphotericin B ; therapeutic use ; Antifungal Agents ; therapeutic use ; Chemical and Drug Induced Liver Injury ; prevention & control ; Fluconazole ; therapeutic use ; Humans ; Itraconazole ; therapeutic use ; Laryngeal Diseases ; drug therapy ; etiology ; pathology ; Mycoses ; drug therapy ; etiology ; pathology

OBJECTIVE:To observe the clinical efficacy and safety of enhanced immunosuppressive therapy in the treatment of refractory nephrotic syndrome (RNS). METHODS:Totally 76 RNS patients were selected from 2 hospitals during Jan. 2012-Mar. 2015,and then divided into control group and observation group according to random number table,with 38 cases in each group. Two groups were given Prednisone acetate tablet 50 mg,qd;decreasing to 15 mg,qd,after urine protein returned to normal. Based on it,control group was given Leflunomide tablets 50 mg,qd;decreasing to 20 mg,qd,3 days later. Based on con-trol group,observation group was additionally given Mycophenolate mofetil dispersible tablet 750 mg,bid;decreasing to 500 mg, qd,3 months later. Both groups were treated for 6 months. Clinical efficacies,follow-up recurrence rate as well as renal function in-dexes and inflammatory cell factors before and after treatment,and the occurrence of ADR were compared between 2 groups. RE-SULTS:The total response rate of observation group(92.11%)was significantly higher than control group(73.68%),and fol-low-up recurrence rate(5.26%)was significantly lower than control group(23.68%),with statistical significance(P<0.05). Be-fore treatment,there was no statistical significance in renal function indexes or inflammatory cell factors between 2 groups(P>0.05). After treatment,24 h urinary protein quantification,urinary IL-6 and IL-8 levels of 2 groups decreased significantly,while the content of serum protein increased significantly;the observation group was significantly better than the control group,with sta-tistical significance(P<0.05). There was no statistical significance in the content of serum creatinine between 2 groups before and after treatment(P>0.05). The incidence of ADR in the control group and the observation group was 34.21% and 44.74% respec-tively,without statistical significance between 2 groups(P>0.05). CONCLUSIONS:Enhanced immunosuppressive therapy in the treatment of RNS can improve renal function,reduce inflammatory reaction and long-term recurrence risk,and have good therapeu-tic efficacy and safety.

Objective To report the clinical results of treatment of tarsometatarsal joint injuries by close reduction and percutaneous screw fixation. Methods From January 2003 to June 2005, 26 cases of tarsometatarsal joint injuries were treated with close reduction and percutaneous screw fixation. They were 13 cases of middle column injury, seven cases of medial-middle column injury and six cases of three-column injury according to Myerson’ s classification. Results In this series all the wounds healed primarily with a mean operative time of 40 min ( 30 to 70 min) and a mean incision length of 5 mm. A mean follow-up of 11.4 months (ranging from 6 to 17 months) revealed a mean score of 87 according to American Orthopaedic Foot & Ankle Society (AOFAS) midfoot scoring system. All the patients returned to normal daily life after a mean time of 5.3 months (3 to 11 months). Conclusions Tarsometatarsal joint injuries can be treated well by close reduction and percutaneous screw fixation with less operative lesion and lower incidence of soft tissue complications. Reduction of the middle column with screw insertion along the direction of the Lisfranc ligament is the key to reestablishment of the stability of the tarsometatarsal joint complex.

The management of medical equipment is one of the important parts in hospital management,which management quality can directly affect the hospital central task and disciplines construction.How to strengthen the management of medical equipment are studied from the plan of medical equipment,the purchase,book building and application four aspects.

Objective To explore the change and its clinical significance of neuron-specific enolase (NSE) and S-100? in serum and hippocampus tissue in rats with epilepsy induced by Kainic acid (KA).Methods 180 Wistar rats were randomly classified into control, KA and carbamazepine (CBZ) group, and the later two groups were further divided into 6 sub-groups (1 h, 4 h, 12 h, 24 h, 48 h and 72 h ) according to epileptic attack at different time point. The NSE and S-100? concentrations in serum and homogenate of hippocampus were determined by radioimmunity assay (RIA) and enzyme linked immunosorbent assay (ELISA), respectively.Results The concentrations of NSE and S-100? were dynamic change during 72 h after epileptic attack in serum and hippocampus homogenate, and the changes were synchronous. The concentrations reached peak at 12 h. Both NSE and S-100? concentrations in KA and CBZ group were obviously higher than those in control group ( P

Objective To investigate the value of magnetic resonance diffusion weighted imaging (DWI) and apparent diffusion coefficient (ADC) in detection of central gland prostate cancer (CGPCa).Methods Routine MRI and DWI examination were performed in 25 CGPCa patients and 30 benign prostate heperplasia (BPH) patients.Then the ADC value was measured according to the image of the lesion after DWI,and the receiver op erating characteristic (ROC) curve was analyzed to select the best ADC value for diagnosis of CGPCa.Results The ADC value of CGPCa (0.89±0.12×10-3 mm2/s) was smaller than that of BHP (1.43±0.12x10-3 mm2/s),and the dif ference was statistically significant (P＜0.05).When the cut off point of ADC value was set at 1.192×10-3 mm2/s,the sensitivity was 80.0％ and the specificity was 85.0％.Conclusion DWI and ADC have clinical application value in diagnosis of CGPCa.