AIM: To investigate the chemical constituents of Shuanghuanglian Injection(Flos lonicerae japonicae, Radix scutellariae, Fructus forsythiae). METHODS: Chromatography and spectral analysis were used respectively to isolate and identify the constituents. RESULTS: Six compounds were isolated from Shuanghuanglian Injection which included baicalin(Ⅰ)、quercitrin(Ⅱ)、hyperoside(Ⅲ)、rutin(Ⅳ)、quercetin(Ⅴ) and luteolin(Ⅵ). CONCLUSION: The other five compounds were first isolated and identified in Shuanghuanglian Injection except baicalin(Ⅰ).

Objective To construct and evaluate the real-time fluorescence quantitative polymerase chain reaction for detecting IL-2R? mRNA in ankylosing spondylitis(AS) based on TaqMan technique.Methods A pair of primers and a TaqMan probe were designed by sequence in GenBank.Total RNA isolated from the fresh peripheral blood monocytes (PBMC) of homo sapiens was amplified by the real-time FQ-RT-PCR.The product was collected by agarose gel electrophoresis and sequencing analysis then ligated with pUCm-T.The recombined plasmid was transcribed to cRNA by T7 RNA polymerase in order to prepare serial standard materials.A new method was created to quantify IL-2R? mRNA in ideal condition.Sensitivity, reproducibility and efficiency were evaluated and used, combined with sIL-2R,for clinical application of AS.Results The linear range was (7~107) cells/ml.The intra-and-inter-assay coefficient variation was 8.4% and 9.6% respectively. Recombined plasmid contained the target fragment was specific and accurate by BLAST.Standard reference was constructed successfully.The RT-PCR product in AS with HLA-B27 positive groups was higher than that with HLA-B27 negative groups and health controls (P0.05).The sensitivity of IL-2R? mRNA was 96.7%.Conclusions Real Time FQ-RT-PCR of IL-2R? mRNA is constructed successfully.This is an easy,rapid,sensitive, accurate and reliable method for quantifing IL-2R? mRNA. There is highly statistical significance, compared with sIL-2R, on the expression of IL-2R? mRNA and inflammatory states between AS and control group and effective information for administration of patients.

Objective To investigate the indication, occasion and risk predication of cesarean hysterectomy and interventional treatment. Methods The clinical data of 9 patients with low cesarean section postpartum hemorrhage that underwent hysterectomy after interventional treatment from January 2012 to January 2013 were analyzed which included surgical indication, disease characteristics, procedure time, bleeding volume and other parameters. Results The most common surgical indication for cesarean that caused postpartum hemorrhage was dangerous placenta previa, accounting for 56%, followed byⅢdegree of placental abruption and preeclampsia. The surgical bleeding of cesarean accounted for 62%-70%of the total amount of bleeding. The total time of rescue was 7.4 ± 1.4 hours. The total amount of bleeding was 7 100 ± 2 250 mL. The occurrence of coagulation disorder was significantly correlated with surgical time and amount of blood loss. Conclusion For postpartum hemorrhage caused by cesarean section with risk factors such as scar uterus, placenta previa, placental abruption, many pregnant prolific and others, we must relax indications of hysterectomy. With future treatment potential, the early intervention of postpartum hemorrhage also plays an important role in reducing hysterectomy rate, reducing intraoperative and postoperative blood loss.

OBJCTIVE:To determine the content of Gentiopicrin in Qianggan capsules by HPLC.METHODS:The sample was separated on Diamonsil TM C18 (200 mm?4.6 mm,5 ?m) with mobile phase consisted of methanol-water (23∶77) at a flow rate of 1.0 mL?min-1.The detection wavelength was set at 254 nm.RESULTS: The linear range of Gentiopicrin was 0.018 59～0.232 40 ?g(r=0.999 6) with an average recovery of 96.59%(RSD=1.35%,n=6).CONCLUSION: The meth-od developed is accurate,stable and reproducible,and it provides basis for the improvement of the quality standard of Qianggan capsules.

Objective: By building database model, to put forward measures to solve the shortage of basic drugs. Methods: There were five factors influencing medicine shortage have been researched, collecting data, using SPSS 18.0 software to build the database model of different parts, and then analyzed the existing problems and inner link, scientifically came up with the basic framework and main solutions of guarantee measures on shortages of essential medicines. Results: The database model of country and six areas in it have been built, and the influence factors of drug shortage in different region have been analyzed, and raised five aspects measures to secure reliable supplies of drugs, including selection, bid and procurement; production and flow; reserve and adjust;pricing and pay;use training. Conclusion: This study extends the application of database, enriches research ideas of drug policy, and the policy put forward to avoid drug shortages to a certain extent.

Objective To analyze the effect of Yaotongning capsule in the treatment of lumbar osteoarthritis patients.Methods From March 2013 to March 2015 in our hospital, 80 cases of lumbar osteoarthritis patients according to the digital table method were divided into two groups: the control group and the experimental group.The patients of the control group were given Bitongning, and the patients of the experimental group were given Yaotongning capsule, and the therapeutic effect, serum indexes and Japanese Orthopaedic Association ( JOA ) of two groups were compared. Results The curative effect of the experimental group was higher than control group(95.00%vs.67.50%) , the JOA score of the experimental group was higher than control group [(27.46 ±1.07)points vs.(21.06 ±1.89)points] (P<0.05).After treatment, the MMP-3(65.28 ±4.37) ng/L and IL-1β(12.43 ±1.01) ng/L of the experimental group were significantly lower than MMP-3(79.56 ±5.36) ng/L, IL-1β(16.44 ±1.03) ng/L of the control group, and the difference was significant (P<0.05).Conclusion The lumbar osteoarthritis patients with Yaotongning capsule has obvious effect, and the function of lumbar intervertebral joint improves obviously, and the serum index is normal, safe and reliable.

Objective:To investigate and assess the systemic functional training in the prevention of lower limb trauma fracture surgery knee stiffness effect. Methods: Select General Hospital of PLA (301 Hospital) traumatic lower extremity Department of orthopedics from 2009 since April admissions of fractures in patients with 126 cases, according to the nursing methods of postoperative patients were divided into experimental group 63 cases and control group 63 cases, control group was given routine nursing after traumatic fracture of lower limb, the experimental group received training rehabilitation care plan and patient in the control group. Postoperative follow-up, the time of 5 months-2 years, two groups of patients were muscle strength, extension and flexion degree and the patients with complications were analyzed statistically, and the comprehensive evaluation of knee joint function recovery of patients. Results:The postoperative knee flexion strength and degree than the control group;experimental group, the complication rate was 15.9%in the control group of patients swelling after seven cases, the complication rate was 28.6%; comprehensive evaluation knee function two groups of patients in the experimental group was 82.5% excellent (52/63), good rate of the control group was 65.1% (41/63);said comparison items were statistically significant differences (x2=12.6, P<0.05). Conclusion: Lower limb trauma fracture surgery rehabilitation for patients with systemic functional training rehabilitation care, joint function recovery is remarkable, scientifically valid methods, should be further promoted.

To observe the efficacy and safety of mini-percutaneous nephrolithotomy with Neodymium: Yttrium Aluminum Garnet (Nd-YAG) laser in the treatment for upper urinary tract stones, from December 2005 to September 2006, 31 patients with renal stones, 15 patients with ureteral stones and 7 patients with renal and ureteral stones underwent mini-percutaneous nephrolithotomy with Nd-YAG laser by combination of rigid ureteroscope and flexible ureteroscope under B-ultrasound guidance. Clinical data including operation time, lithotripsy time, complications and stone-free rate were analyzed retrospectively. Our study showed that the percutaneous renal access (F14-F18) was successfully established under B-ultrasound guidance in all cases. Immediate phase: lithotripsy was performed in 47 cases through single tract, and in one case through two tracts. Delayed phase II lithotripsy was done in 5 cases of renal stones. Operation time ranged from 55 to 180 min with an average time of 100+/-15 min. Lithotripsy time was from 25 to 135 min and the average lithotripsy time was 65+/-11 min. No severe complications occurred in our series. Complex renal stones were cleared in 34 of 38 cases (89.5%). All ureteral stones were completely removed in 15 cases (100%). It was concluded that mini-percutaneous nephrolithotomy with Nd:YAG laser for the treatment of upper urinary tract stones by combination of rigid ureteroscope and flexible ureteroscope has the advantages of effectiveness, mini-invasion, shorter operative time and safety.

This study examined the predictive value of plasma cystatin C, creatinine and estimated glomerular filtration rate (eGFR) as risk factors for cardiovascular disease in Chinese. Plasma cystatin C and creatinine were measured in 466 coronary heart disease (CHD) patients recruited from 4 hospitals and 349 healthy controls from local communities in Wuhan, China. Cockroft-Gault formula was used to estimate the glomerular filtration rate (GFR) after adjusting for body surface area. With each measure, the study population was divided into quintiles. The results showed that the patients had significantly higher levels of plasma cystatin C, creatinine, and lower level of eGFR than controls. Lower eGFR was associated with a higher risk of cardiovascular events. As compared with the first (highest) quintile, the hazard ratios (and 95% CIs) after multivariate adjustment for CHD were as follows: third quintile, 2.98 (1.54-5.78); fourth quintile, 3.34 (1.58-7.09); fifth quintile, 4.37(1.84-10.35). With higher cystatin C quintiles (≥1.00 mg/L and ≥1.17 mg/L), the hazard ratios for CHD were 2.16 (1.23-3.81) and 2.34 (1.25-4.38), similar to those of creatinine 2.21 (1.21-4.03) and 2.03 (1.07-3.84). However, it was plasma cystatin C not eGFR or creatinine had stronger association with ischemic stroke. The highest quintile had the hazard ratio of 4.51 (1.45-14.08) after multivariate adjustment. It was concluded that plasma cystatin C, associated with renal function, is not an independent risk factor for cardiovascular disease. eGFR is a better risk predictor for CHD than plasma cystatin C and creatinine. But for ischemic stroke, plasma cystatin C is a better risk factor than creatinine and estimated GFR.

Eight-year medical education is an important way to train high-level medical students.According to the eight-year medical educational characteristics of neurosurgery,with reference to the teaching achievement both in domestic and foreign medical schools in conjunction with the characteristics of military medical university students,eight-year medical student clinic practice of neurosurgery has been improved through the application of clinical teaching,and achieved good teaching effect.