In order to make the comprehensive evaluation work of intellectual property of TCM industry more scientific and reasonable in our country, promote the protection of intellectual property of TCM industry, based on the survey of the domestic and foreign literature about evaluation of intellectual property and TCM industry, this article built the evaluation index system of intellectual property of TCM industry from five aspects:the number of intellectual property, the quality of intellectual property, the development of intellectual property, the implementation of intellectual property, and the management of intellectual property, and elaborated each index in detail.

Objective　To research the anterior corneal stromal puncture therapeutic effect for bullous keratopathy.Methods　The causes of the bullous keratopathy of the 11 cases were analysed and the bullous keratopathy was cured by the anterior stromal puncture methods.Results　Eleven cases were followed up for three months.The symptoms were relieved in all cases. The corneal bullous disappeared.Conclusion　The cases of the bullous keratopathy caused by mechanical or physical factors can be cured more effectively by using anterior corneal stromal puncture therapeutics.

NO is an important signal factor existing in expiratory air of normal person.Different kinds of airway inflammation can cause the increase of NO,especially eosinophilic inflammation.Fractional exhaled nitric oxide(FENO) can result in airway inflammation,which is noninvasive,repeatable and easily performed.It can be very helpful in the diagnosis and differential diagnosis of airway symptoms such as recurrent wheezing and chronic cough.It can also help determine the state of the disease and prognosis.It is a very useful tool in the assessment and management of airway diseases,especially asthma.

Zinc supplementation can reduce the mean duration,the recurrence rate and mortality of diarrhea.Nonetheless,it has no effect on stool frequency or stool output.Zinc supplementation results in different effort for the difference of the duration of diarrhea,pathogens,zinc type and doses of zinc,and shows no effect in infants younger than 6 months.Using iron as a co-intervention can reduce the effect of zinc,but vitamin A or ORS have a synergistic effect with the meeting.Average baseline zinc levels did not contribute to variations in the effect size.Zinc supplements are unlikely to improve compliance with the treatment for diarrheal disease.Longer course,zinc alone,malnutrition,diarrhea,breast-feeding children are prone to vomiting after oral administration of zinc.

AIM: To analyse the main chemical constituents of Shuanghuanglian Injection Power(Flos lonicerae japanicae,Radix scutellariae and Fructus forsythiae) by high performance liquid chromatography-electrospray ionization-mass/mass spectrometry. METHODS: The sample solution was analysed by a Zorbax C_18 column with a gradient mobile phase comprised of acetonitrile and water.Electrospray ionization mass spectrometry detector was used,full-scan detection mode was evaluated for the identification of the liquid chromatography peaks. RESULTS: To analyse the mass spectrum of every liquid chromatography peaks,we identified 18 molecular mass from the ion chromatogram of Shuanghuanglian Injection Powder,9 compounds were identified,including quinic acid,caffeic acid,three monocaffeoylquinic acids,dicaffeoylquinic acid,forsythin,forsythoside and baicalin. CONCLUSION: Quinic acid is identified from Shuanghuanglian Injection Power for the first time.High performance liquid chromatography-mass/mass spectrometry is emerging as an important tool in traditional Chinese medicine.

Objective To explore the diagnostic value of carcino embryonic antigen (CEA),squamous cell carcinoma antigen(SCC),human papilloma virus-E7 (HPV-E7) in cervical carcinoma.Methods A total of 107 cases of women patients treated in hospital from July 2013 to July 2015 accorded to the pathological examination results were divided into cervical cancer group 60 cases and CIN group 47 cases,another 50 cases of healthy people were selected as control group, and serum expression levels of HPV-E7, CEA and SCC in the three groups were detected by enzyme-linked immunosorbent assay.Results The serum HPV-E7, CEA and SCC in cervical cancer group were significantly higher than those in CIN group and control group (P<0.05).There was no significant difference in serum HPV-E7, CEA and SCC between CIN group and control group.The levels of serum HPV-E7, CEA and SCC in stage I-II were significantly lower than those of stage III-IV in patients with cervical cancer, and the difference with statistically significant between two groups(P<0.05).The area under the ROC of HPV-E7 was significantly higher than that of CEA and SCC (Z=2.914,2.951, P<0.05), and there was no significant difference in the area under the ROC between CEA and SCC (Z=1.580,P=0.057).Conclusion The serum HPV-E7, CEA and SCC in cervical cancer patients are significantly higher, the diagnosis value of HPV-E7 is higher which is expected to become one of the effective indicators of cervical cancer diagnosis.

Objective To explore the clinical effect of limited internal fixation combined with miniature double side external fixing support in the treatment of midfoot fracture dislocation.Methods From March 2010 to March 2015,200 patients of midfoot fracture dislocation in our hospital were randomly divided into the control group and the observation group according to random figure table,100 cases in each group.The patients of the observation group were given limited internal fixation combined with miniature double side external fixing support treatment,and the patients of the control group were treated with conventional therapy.The clinical effects of the two groups were observed and compared.Results The operation time and wound healing time of the observation group[(67.46 ± 4.53)min,(837 ±3.43)weeks]were significantly shorter than those of the control group[(113.25 ±5.63)min, (16.44 ±5.63)weeks](t =8.37,12.24,all P <0.05).After different surgical treatment,the incidence rate of lim-ited activity of the observation group (0.00%)was significantly lower than 30.00% of the control group.The disease recovery excellent rate of the observation group (100.00%)was significantly higher than that of the control group (70.00%),and the difference was statistically significant (χ2 =35.29,P <0.05).Conclusion For midfoot frac-ture dislocation patients,limited internal fixation combined with miniature double side external fixing support treatment is very good to patients,which can correct fracture and dislocation,fast recovery,decrease infection opportunity,greatly reduce the patients'pain,it is worthy of widely used in clinic.

Traditional Chinese medicine has advantages in treating chronic glomerulonephritis. According to the articles in recent years, the therapeutic methods of TCM include not only syndrome differentiation, but also symptomatic treatment, modification of the chief prescriptions, Chinese traditional patent medicine, single Chinese herb, and combined internal and external treatment, which all have achieved some remarkable effects. However, the majority of articles were about personal experience and clinical observation which lack experimental study on the therapeutic mechanism. Therefore, the results of relative researches should be under objective evaluation. A large number of experimental researches on the therapeutic mechanism and standardized clinical observation need to be carried out in the future.

Objective To study the distribution of clinically separated Hemophilus (H .) influenzae and its drug resistance situa‐tion .Methods Among 2 663 specimens of screening qualified and positive culture collected from the outpatients ,inpatients and e‐mergency patients of our hospital from January to December 2014 ,including 1 895 specimens of lower respiratory tract ,666 blood specimens and 102 urinary secretion specimens ,438 strains of H .influenzae were isolated .The cephalosporin nitro thiophene paper test strip was used to test its beta lactamase and the K‐B method was used to detect its sensitivity to 8 kinds of antibacterial drugs . The testing data were statistically analyzed by using WHONET 5 .6 software .Results Among 438 strains of H .influenzae ,98 .6%was originated from hospitalized patients ,1 .4% from the outpatient department ;176 isolated strains were originated from children , accounting for 40 .18% ;194 strains (44 .29% ) were from elderly patients ,434 strains(99 .09% ) were originated from the lower re‐spiratory tract specimens .The resistance rate to compound sulfamethoxazole was highest and reached 82 .90% ,which to ampicillin and tetracycline were 29 .00% and 43 .40% respectively ;but which to cefuroxime ,ceftriaxone ,ciprofloxacin ,azithromycin ,imipenem were below 2 .00% .The β‐lactamase positive rate was 43 .40% ,in ampicillin resistant strains ,1 .57% of strain was negative in β‐lac‐tamase test .The resistance to ampicillin and enzyme production rate had no significant differences between the children isolated strains and adult isolated strains (42 .43% ,42 .40% vs .44 .00% ,44 .00% ) .Conclusion H .influenzae is mainly isolated from low ‐er respiratory tract specimens ;which is dominated by children and elderly patients ;the second and third generations of cephalospo‐rins and azithromycin can serve as a clinical preferred drugs ;β‐lactamase is the primary mechanism of resistance to ampicillin .

Objective To analyze the characteristics and related factors of the incidence of intestinal infectious diseases in children.Methods 180 children with intestinal infectious diseases who were treated from July 2012 to July 2015 in our hospital were radomly selected.Meanwhile,180 healthy children were randomly selected from pediatric hospital examination as control group.Then,according to the clinical data and epidemiological data of these children,the number of cases were compared,and these children were from different age,different regions and different types of infections.The incidence factors of univariate and multivariate Logistic analysis were compared.Results In terms of age,patients younger than 1 year of age were 105 cases,between the ages of 1 -3 years were 37 cases, patients older than 3 years were 38 cases,the proportion of younger than 1 year was 58.3%,the proportion of patients between 1 -3 years of age was 20.6%,the proportion of older than 3 years of age was 21.1%,the proportion of patients younger than 1 year of age compared with the proportion of patients with the other two groups had significant differences (P <0.05).In the area,117 cases were from rural areas and the incidence rate was 65.0%,63 cases were from the city,and the incidence rate of the city was 35.0%,which was significantly lower than the incidence of rural areas,the difference was statistically significant (P <0.05).On the type of infection,parenteral infection was 24 cases,bacterial infection was 75 cases,viral infection was 81 cases,parenteral infection rate was 13.3%,bacterial infection rate was 41.7%,virus infection rate was 45.0%,parenteral infection was significantly lower than the other two infection prevalence groups,the difference was statistically significant (P <0.05 ).After univariate analysis, observation and control groups were compared,showed that children washing their hands before meals,children washing their hands after stool,parents washing their hands after stool,no tableware disinfection,ground nails,indoor flies and intestinal infectious diseases in children were concerned (P <0.05).After multivariate Logistic analysis,OR values of no tableware disinfection and indoor flies were greater than 1,they were risk factors,and ground nails,OR value of children and children wash their hands before meals and after hand washing were less than 1,they were protective factors.Conclusion Pediatric intestinal infectious diseases mainly occur in infants less than 1 year old, and the incidence has temporal and spatial distribution,multiviral and bacterial infections,the analysis by the relevant factors,including age,time,space and infectious pathogens.And children washing their hands before meals,washing their hands after stool,parents washing their hands after stool,no tableware disinfection,ground nails,indoor flies and intestinal infectious diseases in children -related,but not sterilized tableware and interior flies belong to risk factors and attendance nails,children and children wash their hands before meals and after hand washing belong to protection factor.