AIM:To study the effect of Ketangte2 (KTT2)on reducing blood glucose and its mechanism.METHODS:Adrenalin and streptozocin were used to induce hyperglycemia in two mice models,and then treated them with glybenzcyclamide (50 mg/kg),three different dose of KTT2 and normal saline(NS)(0.1 ml/10 g weight)for 15 days.And other healthy mice were set up as control group,During the experiment,the fast blood glucose(FBG),insulin was measured in different time.The pancreas and islets of diabetic mice induced by STZ were studied by pathologic section.RESULTS:In the adrenalin-induced model group,KIT2 could lighten hyperglycemic reaction,which Was significant different from that of model group(P＜0.05 or 0.01),while KTT2 high,middle dose group could decrease blood glucose in streptozocin-induced hypoglycemic mice obviously(P＜0.01).Histological examination showed that pancreatic island number in pancreas in KTT2 groups increased in comparison with the model group.CONCLUSION:KTT2 can obviously reduce the blood glucose in the two kinds of animal models of hyperglycemia (P＜0.05 or 0.01).Its mechanism may be related to the protective effect on islet β cells,thereby increase the insulin secretion.

Objective　The effects of endotoxemia on hepatic energy metabolism and hepatic tissue blood flow were studied on the rats with obstructive jaundice.Methods　 After biliary obstructed and decompressed the rats were injected endotoxin,then hepatic tissue blood flow and energy metabolism were determined.Results　Hepatic blood flow decreased and hepatic energy metabolism lowered in rats with obstructive jaundice.Hepatic blood flow decreased markedly and hepatic energy metabolism lowered further due to endotoxemia. Afeter 4 weeks of jaundice hepatic energy metabolism did not recover.Conclusion　The decrease in hepatic tissue blood flow affected notably hepatic energy metabolism in obsturctive jaundice.When obstructive jaundice with endotoxemia happened,hepatic tissue blood flow markedly deceased and energy metabolism lowered further.

IQGAP1 is a scaffold protein which binds to various signaling and structural molecules.By interacting with target proteins,IQGAP1 participates in integration of signaling pathways and regulation of cellular functions.More and more evidences suggest that IQGAP1 plays an important role in tumorigenesis by promoting cell proliferation and transformation,weakening cell adhesion and stimulating cell motility and invasion.

Objective To investigate the pathogens and drug resistance of bacteria in children with pneumonia in Chongqing by using antibiotic reasonably.Methods Bacteria identification antibiotics sensitivity test were performed in samples of lower respiratory tract secretions from 780 cases by microscan automatic analysis machine.At the same time,respiratory virus,chlamydial and mycoplasmal pneumoniae were tested.Results The pathogens were found in 433 cases.Three hundred and sixty-four strains bacteria were isolated,116 cases were infected by virus and 26 cases by mycoplasmal pneumoniae.The resistant rates of staphylococcal and Klebsiella pneumoniae were high.The resistant rate of Streptococcus pneumoniae to penicillin was high.Haemophilus parainfluenzae was sensitive to cephalosporins.Conclusions In Chongqing,children′s pneumonia is mainly caused by bacteria.The sensitivity of antibiotic varies from different bacteria.Finding pathogens and monitoring drug resistance have taken great advantage to antibiotic selection and have prevented rapidly growing of resistant strains.

The botanical Origin, macroscopical and microscopical characteristics and physical chemical tests for the idcntification of Tianhusui (Hydrocotyle sibthorpipides Lam. ) and Potongqian (H.sibthorpioids Lam. var. batrachium(Hance) Hand-Mazz.) were reported to provide abasis for the identification of the drug and revision of the drug standard.

Totally 1 patient were selected from Beijing Xishan Institute for Neuroregeneration and Functional Recovery in Shijingshan District in May 2006. The patient received acupuncture treatment in trideca-ghost points after olfactory ensheathing cells (OECs) transplantation. Detailed acupuncture method and acupuncture point were as follows: Acupuncture was performed from left side on Shuigou (DU 26), Jiache (ST 6), Fengfu (DU 16), Chengjiang (RN 24), Shangxing (DU 23), oblique needling from Shuigou to mittele muschel 0.3 cun. Needle insertion on Chengjiang was from left to right, for 1 minute without retaining needle. Then acupuncture on Shaoshang (LU 11), Daling (PC 7), Jianshi (PC 5), Quchi (LI 11) of both sides, and lower limbs: Yinbai (SP 1) and Shenmai (BL 62) was conducted. Acupuncture on Jiache and Quchi was retained until the patient vellicated limbs. Punctured on Dicang (ST 4) if the patient had spasm on angulus oris, about 20 minutes every time, once a day. After the treatment, the patient had some improvements in the consciousness and electrophysiology. Significant pain reaction appeared, and the endings of extremities had some little movement. The patient could turn head and twist shoulder. Evoked potential in electromyogram (EMG) had certain amelioration.

The interaction between the immune system and nervous sytem affcts nervous function.There is a close relationship between the episode of epilepsy and immunity,the molecular basis of which is the network of cytokines.In order to quest new methods of contolling epilepsy by regulating the network of cytokines,the roles of several cytokines in the ocurrence and onset of epilepsy were reviewed in this article.

OBJECTIVE:To evaluate the initial and acquired resistance level of lung tuberculosis in Shenzhen.METHODS:Culture-positive of sputum specimens of1948cases of lung tuberculosis were collected in Shenzhen from1999to2002and the resistance of tubercle bacillus in sputum-positive to four anti-tuberculosis drugs(isonicotinylhydrazide INH,streptomycin SM,rifampin RFP,Ethambutol EMB)were tested by WHO guideline.RESULTS:The total rate of tubercle bacillus to drug resistance was35.06%,initial drug resistance rate34.01%,initial multi-drug resistance rate4.97%,acquired re?sistance rate45.51%,and acquired multi-drug resistance rate12.92%.Acquired resistance rate of tubercle bacillus to each of4drugs was on the rise to different extents as compared with initial resistance rate.The rise was39.91%for SM,94.07%for INH,44.04%for EMB,and118.18%for RFP.CONCLUSIONS:The initial drug resistance rate ranks higher in Shenzhen as compared with the average level in the whole nation,resistance rates of RFP and INH double after combined administration.

Objective:To explore the microanalysis method used to evaluate the solution-focused brief coun-seling (SFBC)interview characteristics of the skilled trainees and novices.Methods:Five skilled SFBC trainees, who had learned and practiced of SFBC for not less than 5 years,and 9 SFBC novices,who had a total of learning of SFBC for no more than 3 months,were recruited.The skilled trainees'5 interview recordings and 9 interview re-cordings of the novices were collected and coded.Then the formulations and questions used in the two groups'inter-views were compared with each other,and the different nature of language (positive,negative or neutral)used by them were also analyzed.Results:The numbers of complex formulations used by skilled trainees were more than the novice's [(4.4 ±2.5)vs.(1.6 ±1.1),P <0.05].And the percentage of opening questions was significant higher than novice's [(0.83 ±0.14)vs.(0.61 ±0.18),P <0.05].The numbers of positive utterances used by the skilled trainees were much more than the novices'[(16.0 ±7.0)vs.(8.7 ±2.4),P <0.05].Conclusion:The microanaly-sis method could be effectively used to distinguish the characteristic differences between the skilled trainees and the novices in their formulations,opening questions and positive utterances,during their respective interviews.And it could be effectively used to evaluate the interview skill levels of the skilled trainees and novices.