IQGAP1 is a scaffold protein which binds to various signaling and structural molecules.By interacting with target proteins,IQGAP1 participates in integration of signaling pathways and regulation of cellular functions.More and more evidences suggest that IQGAP1 plays an important role in tumorigenesis by promoting cell proliferation and transformation,weakening cell adhesion and stimulating cell motility and invasion.

AIM:To study the effect of Ketangte2 (KTT2)on reducing blood glucose and its mechanism.METHODS:Adrenalin and streptozocin were used to induce hyperglycemia in two mice models,and then treated them with glybenzcyclamide (50 mg/kg),three different dose of KTT2 and normal saline(NS)(0.1 ml/10 g weight)for 15 days.And other healthy mice were set up as control group,During the experiment,the fast blood glucose(FBG),insulin was measured in different time.The pancreas and islets of diabetic mice induced by STZ were studied by pathologic section.RESULTS:In the adrenalin-induced model group,KIT2 could lighten hyperglycemic reaction,which Was significant different from that of model group(P＜0.05 or 0.01),while KTT2 high,middle dose group could decrease blood glucose in streptozocin-induced hypoglycemic mice obviously(P＜0.01).Histological examination showed that pancreatic island number in pancreas in KTT2 groups increased in comparison with the model group.CONCLUSION:KTT2 can obviously reduce the blood glucose in the two kinds of animal models of hyperglycemia (P＜0.05 or 0.01).Its mechanism may be related to the protective effect on islet β cells,thereby increase the insulin secretion.

BACKGROUND:In comparison with stem cel s from other tissues, adipose-derived stem cel s (ADSCs) are not only abundant and easy to harvest but also have the strong abilities to proliferate and differentiate. These make ADSCS the promising stem cel s in the field of oral tissue regeneration. OBJECTIVE:To summarize the biological features of ADSCs and the development of functions as stem cel s in the oral tissue regeneration. METHODS:We took“adipose-derived stem cel s, oral tissue regeneration, bone tissue engineering, periodontal tissue regeneration, bone defects”as the key words in Chinese and English, respectively, to retrieve the related literatures from PubMed, CNKI, Wangfang databases during 2000 to 2016 based on internet search. RESULTS AND CONCLUSION:In vitro studies on periodontal tissue regeneration have shown that ADSCs can promote the regeneration of bones, fibroblasts, blood vessels around the periodontal area, and improve the healing abilities of injured tissues. In the study of craniofacial bone regeneration, ADSCs can successful y repair massive bone loss due to the surgeries of tumor or accidents. ADSCs have a promising role in the oral tissue regeneration, but further studies wil be required because of the problems of immunogenicity and safety.

Objective To evaluate the effect and mechanism of ginseng polysaccharides injective solu-tion on invasive ability of human breast cancer MCF-7 cells.Methods Human breast cancer MCF-7 cells were treated with different concentrations of ginseng polysaccharides injections(12.5μL/mL,25μL/mL,50μL/mL or 100μL/mL),and phosphate buffer saline was served as control.The invasive ability,transfer ability and protein expression of matrix metalloproteinases-2 ( MMP-2 ) and matrix metalloproteinases-9 ( MMP-9 ) were determined via transwell matrigel invasive test,scratch test and Western Blot,respectively.Results Ginseng pol-ysaccharide injection on invasive ability and transfer ability in vitro of MCF-7 cell had significant role in inhibi-ting effect,and showed dose and time dependent.After ginseng polysaccharides injection treatment,the MMP-2 and MMP-9 protein expression significantly reduced,and showed dose and time dependent manner.Conclusion Ginseng polysaccharides injection is able to inhibit the invasion and transfer in MCF -7 cells.Moreover,theregulation of MMP -2 and MMP -9 expression may be involved in this effect.

OBJECTIVE:To investigate the protective effects of adjunctive general anesthesia of dexmedetomidine on cardiac and cerebral ischemia-perfusion injury. METHODS:50 patients,ASA physical status Ⅰ-Ⅱ,undergoing selective tracheal intuba-tion,were randomly divided into dexmedetomidine group (group D) and control group (group C) with 25 cases in each group. The patients in group D received a loading dose of 1 μg/kg of dexmedetomidine by infusion pump before anaesthesia,10 min lat-er by a maintenance dose of 0.5 μg/(kg·h)till the end of surgery;whereas patients in group C received normal saline and same induction. HR,SBP and DBP of 2 groups were monitored at the beginning of operation,10 min after incision,30 min after inci-sion,at the end of operation. Blood samples of vena jugularis interna were taken to determine the plasma levels of cardiac tropo-ninⅠ(cTnⅠ) ,adrenaline and norepinephrine before anesthesia induction (T0),at the end of surgery (T1),6 h after surgery (T2),12 h after surgery(T3),24 h after surgery(T4),48 h after surgery(T5). RESULTS:There was no statistical significance in the levels of HR,SBP and DBP in group D between during operation and at the beginning of operation(P>0.05);those in-dex were significantly lower than those of group C at corresponding time points,with statistical significance(P<0.05). The lev-els of cTnⅠ in group D at T2-T5 were significantly lower than in group C,with statistical significance(P<0.05). The levels of adrenaline and norepinephrine at T1-T5 were significantly lower than group C,with statistical significance (P<0.05). CONCLU-SIONS:Adjunctive general anesthesia of dexmedetomidline can improve cardiac and cerebral ischemia-perfusion injury to certain extent.

The interaction between the immune system and nervous sytem affcts nervous function.There is a close relationship between the episode of epilepsy and immunity,the molecular basis of which is the network of cytokines.In order to quest new methods of contolling epilepsy by regulating the network of cytokines,the roles of several cytokines in the ocurrence and onset of epilepsy were reviewed in this article.

OBJECTIVE:To evaluate the initial and acquired resistance level of lung tuberculosis in Shenzhen.METHODS:Culture-positive of sputum specimens of1948cases of lung tuberculosis were collected in Shenzhen from1999to2002and the resistance of tubercle bacillus in sputum-positive to four anti-tuberculosis drugs(isonicotinylhydrazide INH,streptomycin SM,rifampin RFP,Ethambutol EMB)were tested by WHO guideline.RESULTS:The total rate of tubercle bacillus to drug resistance was35.06%,initial drug resistance rate34.01%,initial multi-drug resistance rate4.97%,acquired re?sistance rate45.51%,and acquired multi-drug resistance rate12.92%.Acquired resistance rate of tubercle bacillus to each of4drugs was on the rise to different extents as compared with initial resistance rate.The rise was39.91%for SM,94.07%for INH,44.04%for EMB,and118.18%for RFP.CONCLUSIONS:The initial drug resistance rate ranks higher in Shenzhen as compared with the average level in the whole nation,resistance rates of RFP and INH double after combined administration.

Objective To investigate the difference of efficacy of interlocking nails and dynamic compression plate in tibial shaft fractures.Methods The clinical data of 137 cases with tibial shaft fractures were reviewed retrospectively.They were divided into two groups according to the various treatments which included interlocking nails group(72 cases with 79 fractures) and dynamic compression plate group(65 cases with 70 fractures).The efficacy,healing fineness rate,function score after operation and complications were compared.Results After operation,the patients were followed up 21.4 months on average.The postoperative callus time,weight-bearing limb time,healing time and postoperative fixation time taken in interlocking nails group was shorter than that in dynamic compression plate group [(2.36 ± 0.46) months vs.(3.12 ± 0.57) months,(2.42 ± 0.51) months vs.(3.14 ± 0.63) months,(7.65 ± 1.29) months vs.(9.33 ±1.54) months,(12.64 ± 3.76) months vs.(16.38 ± 3.15) months],and there was significant difference (P＜ 0.05).The healing fineness rate was 97.5％(77/79) in interlocking nails group,and 92.9％(65/70) in dynamic compression plate group,and there was nosignificant difference (x2 =1.76,P ＞0.05).The incidence of comphcations in interlocking nails group was higher than that in dynamic compression plate group[25％(2H9) vs.12.9％(9H0)],and there was significant difference(P ＜ 0.05).Condmions Interlocking nails have better biomechanical features with high union rate,low infective rate and good recovery of joint function.The clinical result of interlocking nails for the tibial shaft fractures is also better than that of dynamic compression plate.

Objective　The effects of endotoxemia on hepatic energy metabolism and hepatic tissue blood flow were studied on the rats with obstructive jaundice.Methods　 After biliary obstructed and decompressed the rats were injected endotoxin,then hepatic tissue blood flow and energy metabolism were determined.Results　Hepatic blood flow decreased and hepatic energy metabolism lowered in rats with obstructive jaundice.Hepatic blood flow decreased markedly and hepatic energy metabolism lowered further due to endotoxemia. Afeter 4 weeks of jaundice hepatic energy metabolism did not recover.Conclusion　The decrease in hepatic tissue blood flow affected notably hepatic energy metabolism in obsturctive jaundice.When obstructive jaundice with endotoxemia happened,hepatic tissue blood flow markedly deceased and energy metabolism lowered further.