The present study was to investigate whether peripheral NMDA receptors were involved in the persistent nociceptioninduced by subcutaneous (s. C. ) bee venom injection in the conscious rat by using quatitative pain scoring methods, a.c. Bee venom injection into one hindpaw resulted in a persistent, monophasic nociceptive response characterized by continuously flinching.lifting and licking the injected paw for more than I h. The non-competitive. NMDA receptor channel blockers, ketamine and ME-801, were administered s.c. 5 or 20 min after bee venom. Local ketamine injection produced a suppression of flinching reflex by 20. 90±2.88% and 45.76±13.9%, while that of lifting/bcking time by 39. 53±10. 05% and 59.94±5.53%, at doses of 25mmol/L and 50 mmol/L respectively without any motor disturbance, Local MK-801 resulted in an inhibition of flinching reflexby 22.84±3.12% and 49.53±5.35%. While that of lifting/licking time by 17. 49±5.67%and 53.49±3. 87%. At doses of 10μmol/L and 100 ,μmol/L respectively also with no motor disturbance. However, s. C. Administration of ketamine and MK-801 inior region symmetrical to the bee venom injection site on the contralateral hindpaw produced no change in the nociceptive behaviors, suggesting that the analgesic actions of keramme and MK-8Ol were not the result of systemic effects. The present resultsuggests that peripheral NMDA receptors are involved in the production of persistent pain.

BACKGROUND: Serum levels of high-sensitivity C-reactive protein (hs-CRP) can be used to predict cardiovascular injury.OBJECTIVE: To investigate the serum levels of hs-CRP in a rabbit model of atherosclerosis following repeated long-termexposure to positive acceleration and to predict the risk of cardiovascular injury.METHODS: Twenty-four New Zealand purebred rabbits were randomly divided into a positive acceleration group and a controlgroup. Rabbits in the positive acceleration group were exposed to +4 g rotation for 20 seconds with an acceleration of 1 g/s. Therotation interval was 5 minutes and totally three rotations were daily performed for 3 days per week. 0.5 g rotation was addedevery week, and by week 4, +6 g rotation was added, and the exposure lasted for 40 seconds.RESULTS AND CONCLUSION: Exposure to positive acceleration yielded significant effects on serum level of hs-CRP in rabbitswith atherosclerosis compared with before exposure (P < 0.01), but exposure time did not produce effects on serum level ofhs-CRP (P > 0.05). The interaction between exposure to positive acceleration and exposure time also did not produce significanteffects on serum level of hs-CRP (P > 0.05). Hyperplastic foam cells in the aortic tunica intima and the superficial layer of mediaincreased with the prolongation of exposure to positive acceleration. Repeated long-term exposure to positive acceleration maylead to a long-term high serum level of hs-CRP in rabbits.

Relaxation and contraction factors influencing penile erection are produced and released by the central and peripheral nerves as well as intracavernosal sinus gap and vascular endothelial cells. Aging, diabetes, cardiovascular disease and spinal cord injury can influence these factors. Further researches of hyperbaric oxygenation (HBO) on the erectile dysfunction (ED) can provide some theoretical evidences for the clinical treatment of ED.
Erectile Dysfunction ; therapy ; Humans ; Hyperbaric Oxygenation ; Male ; Penis ; physiopathology

Objective　To investigate ideal procedure of reconstruction for the digestive tract after total gastrectomy.Methods:After total gastrectomy,the 25 centimeters length jejunum with vessel pedicle was dissociated and was inosculated between esophagus distal and duodenum proximal end.This procedure is simple and meet the physiological need.Results:28 cases of patients were curved recently without complications and death.All patients wre followed-up long term survey that they had no intestinal symptoms,reverse esophagitis and nutritional deficiency.Their body weights were increased significantly and could take part in labor.Conclusion:This procedure is the ideal reconstruction operation for the digestive tract after total gastrectomy.

OBJECTIVE To determine the association between phenotype and genotype of biofilm in Staphylococcus.METHODS All 156 clinical isolates of coagulase negative Staphylococcus(CNS) and 90 S.aureus(SAU) strains isolating from clinical specimens from Mar 2005 to Jul 2008,as well as 12 CNS strains from nasal swabs of healthy carriers from hospital staff,were collected.Congo red technique was performed to examine slime production.The presence of the intercellular adhesion genes(icaA and icaD) was determined by the polymerase chain reaction method.The phenotype and genotype of biofilm were analyzed.RESULTS Thirty-two out of 168 CNS isolates were biofilm producers(19.0%) while 32 out of 90 SAU isolates were producers(35.6%).All strains positive for icaA positive were also positive for icaD.The 16 strains(10 CNS isolates and 6 SAU isolates) were icaA and icaD positive but do not produced slime.CONCLUSIONS For SAU,the percentage of slime-production strains(35.6%) is higher than that found in CNS(19.0%).The characteristic of biofilm-negative variants having the ica loci might contribute to the dissemination.For clinical isolates of S.epidermidis,ica locus may not be a significant marker discriminating between invasive and contaminating isolates.

OBJECTIVE:The effects of zinc fructose diphosphate(ZnFDP)in different concentrations on the growth of cultured newborn mouse cerebral cortex neurons were observed METHODS:The newborn mice cerebral cortex neurons were cultured and different dosages of ZnFDP were added with final concentrations of 2 5?g/ml,12 5?g/ml and 125?g/ml The convert phase microscope was used to observe the growth of dendrites and cell bodies of neurons The survival neuron count and LDH assay were carried out to investigate the effect of ZnFDP on the growth and development of neurons in different culture periods RESULTS:After 48h,the number and the length of neural dendrites in 12 5?g/ml ZnFDP group were increased but the maximum diameter of cell bodies of neurons showed no change There were no significant differences in all parameters observed between 2 5?g/ml group and control group,while 125?g/ml ZnFDP obviously inhibited the differentiation of neurons The survival neurons on 12 5?g/ml ZnFDP group outnumbered those in the control group after 3d,7d and 10d culture,and the LDH activity in 12 5?g/ml ZnFDP group was lower than that in control group after 7d and 10d culture CONCLUSION:This study suggests that a suitable dose of ZnFDP can promote the growth and development of cerebral cortex neurons

Objective To explore the clinical characteristics of severe hand-foot-mouth disease in children and summarize nursing experience.Method The clinical data of 32 children with severe hand-foot-mouth disease were reviewed and analyzed retrospectively. Results After standard diagnosis and treatment,all cases were cured. The average stay in hospital was(8.2±1.6)d.Conclusion The close observation,prevention of infection,skin and oral nursing are of importance for promoting curative effectiveness and prognosis.

Objective To evaluate retrospectively ECG -gating for the coronary angiography with MSCT. Methods 15 patients were studied with a multislice spiral CT on retrospective ECG -gating,0.5s rotation, and cardial segment reconstruction algorithm. Nine phases were chosen to reconstruct images of coronary arteries. Results In the 230 coronary artery segments,213 (91%)were best visualized at 70%. And 15 (6.4%), 9 (3.8%), and 3 (1.3%) were optimal at 80%, 40% , and 5% of the cycle respectively. Conclusion The coronary arteries usually are best visualized at 70% image reconstruction window of the cardial cycle.

0.05). Oil red O staining demonstrated that lipid droplet number was significantly less in the each adipogenic inductor + extracting solution from Eucommia ulmoides Oliv. groups compared with the adipogenic inductor group. CONCLUSION: Extracting solution from Eucommia ulmoides Oliv. can induce the differentiation of in vitro cultured goat BMSCs into osteoblasts, and inhibit their differentiation into adipocytes.