With the Science Citation Index Expanded(SCIE)and Journal Citation Reports(JCR), the amount and distribution of the originals in Progress in Biochemistry and Biophysics, which were cited by the journals included by SCIE, were analyzed according to method of citation analysis. The average cited number of original articles cited by other researchers is 1.70. The cited authors are from 23 provinces and from other countries. Beijing, Hunan, and Guangdong province are in the front of most cited author's areas. The most frequently cited institute was Xiangya School of Medicine at Central South University. There are 115 citing journals.

There were neither available database nor deep investigation on gene patents or patented genes in China before.But the National Bio-Sequence Database of Chinese Patent(NASDAP,http://nasdap.generank.org/),comprising 123 218 patented bio-sequences within 8 563 Chinese patents,has been constructed now.Genes,microarrays,single nucleotide polymorphisms(SNPs),motifs and any other objects of Chinese patent are involved in,and BLAST-based search or motif scan are carried out to navigate these objects,which can serve as an indispensable bioinformatics platform for setting up of research work,tracing of the advancement in patented genes and facilitating the decision of both applicants and assigners of gene patent.Such platform also gives comprehensive consults on drawing intellectual property strategies in the areas of pharmaceutics,diagnostics and agriculture.

Ergonomics ; Humans ; Occupational Health

Animals ; Endothelium, Vascular ; Humans ; Lipid Metabolism ; Metabolic Diseases

?AIM:To compare the curative effect of conjoint fascial sheath( CSF) suspension and levator muscle resection for moderate or severe congenital ptosis.?METHODS: Forty - three patients ( 74 eyes ) with moderate or severe ptosis were treated by CSF suspension or levator muscle resection randomly, and followed up for 6mo. The normalization rates of the two operations were then compared by statistical method, and the complications of the two operations were analyzed.?RESULTS: The two operations appeared no significant difference on the normalization rate for moderate congenital ptosis (P>0. 05), while the normalization rate of CSF suspension on severe congenital ptosis was significantly higher than that of levator muscle resection (P<0. 05). Less complication was happened in the CSF suspension group than in the levator muscle resection group.?CONCLUSION:CSF suspension is more effective on the treatment of severe congenital ptosis than levator muscle resection, and has advantages such as less trauma, repeatable, and less complication.

Objective: This study aimed to investigate the induction of MxA protein in PBMC treated with various doses of adenovirus type 3(Ad3) and to test the antiviral effect of MxA protein against Ad3 in vitro.Methods:The content of MxA protein in cytoplasm of the peripheral blood mononuclear cells( PBMC) , which were treated with various dose of Ad3 was detected by flow cytometry. The antiviral effect of MxA protein against Ad3 in Hela cells was studied by the microdose cytopathogenic effect inhibition assay. Results: MxA protein that in all the cell groups that were treated with various dose of Ad3 was higher than that of control. 10 ng/ml MxA protein can resist 20TCID50 Aad3. Conclusion: It was suggest that MxA protein that can be induced by Ad3 in PBMC and recombinant MxA can resist Ad3.

Objective To explore the application of students' evaluations of educational quality questionnaire (SEEQ) and related influencing factors in the teaching effectiveness evaluation of medical faculty.Methods 477 Grade 1-4 five-years medical students in China medical university were investi-gated to evaluate the teaching effectiveness.We used the internal consistency and principal component method to test the reliabihty and validity of the scales.We analyzed different influencing factors of educational quality including different gender,grade,student academic achievement and curriculum type with the t-test and AVONA in the SPSS 19.0 software,P＜0.05 was considered statistically.Results The Cronbach's α coefficient of SEEQ was 0.89,the cumulative variance contribution rate was 86.43％,the reliability and validity of the questionnaire were good.Girls scored higher in the teacher-student relationships domain than boys(P=0.022).Students in the lower grade had lower scores in the dimen-sions of the scales (P＜0.05).Clinical courses scored higher in all dimensions of the scales(P＜0.05) than basic medical courses.Outstanding students had higher scores in the sense of the learning value,teacher-student relationships,the scope of teacher's knowledge and curriculum management than nor-ma] students(P＜0.05).Conclusions It is feasible that SEEQ questionnaire is applied in the teaching evaluation of medical facuhy.Medical faculties should carry out teaching innovation according to the characteristics of different students and course type with the targeted method.

This paper introduced the teaching reform in the integrated course of introduction to clinical medicine in China medical university.According to the capacity and quality in the global minimum essential requirements in medical education (GMER) proposed by the Institute for International Medical Education (IIME),we carried out the teaching reform of ‘early exposure to clinic’ in the integrated course of introduction to clinical medicine based on the theory of competency-based education (CBE) and took capacity-building as the teaching objective.We achieved good teaching effect and appraisal by students.

Objective To preliminarily evaluate the performance of glucose detention reagents with three kinds of different chro-mogens and to investigate their anti-interference performance according to NCCLS document.Methods According to the protocol EP10-A2 provided by the National Committee for Clinical Laboratory Standards(NCCLS),the samples with low,middle and high level of glucose were detected by the glucose reagent kits with 3 kinds of different chromogens.The bias,total imprecision,inter-cepts,slope rates,nonlinearities,carryover rate and drifts were calculated.The interference evaluation test was performed according to the document EP7-A2.Results The bias and total imprecision of three kinds of reagent kits were all within allowed ranges.No statistically significant differences were showed in intercepts,slope rates,nonlinearities,carryover rate and drifts.1450 turbidity chyle,5 g/L hematoglobin and 0.03 g/L vitamin C did not interfere with the assay of three kinds of glucose reagent kits with differ-ent chromogens.342 μmol/L free bilirubin,342 μmol/L conjugated bilirubin did not interfere with the detection of reagent with MAOS.Conclusion The glucose detention reagents with three different chromogens have good accuracy and precision,and various performance indexes all conform to the clinical application requirements,reagent with chromogen MAOS is better than other chro-mogenic reagents in the anti-interference performance.

Purpose To exp1ore the expression and c1inica1 significance of epiderma1 growth factor receptor( EGFR)and human epi-derma1 receptor-2(HER-2)in co1orecta1 carcinoma. Methods Immunohistochemistry(IHC)PV-9000 was used to detect the expres-sion of EGFR and HER-2 in 78 cases of co1orecta1 carcinoma. Si1ver in situ hybridization( SISH)was used to detect HER-2 amp1ifica-tion in co1orecta1 carcinoma. Results The positive rates of EGFR in 78 co1orecta1 carcinomas were 69. 23%( 54/78 ). The differ-ences of the expression of EGFR between different depth of invasion group and 1ymph node metastasis group were statistica11y signifi-cant. The differences of the expression of EGFR between different aging group,gender group,tumor size,different histo1ogica1 grading group and Dukes stage were insignificant. The positive rates of HER-2 in 78 cases of co1orecta1 carcinoma were 25. 64%(20/78). The differences of the expression of HER-2 between different depth of invasion group and 1ymph node metastasis group were statistica11y sig-nificant. The differences of the expression of HER-2 between different aging group,gender group,tumor size,histo1ogica1 grading group and Dukes stage were insignificant. The expression of EGFR and HER-2 was positive1y corre1ated. Among the 20 cases of HER-2 protein overexpression,10 cases showed HER-2 gene amp1ification,15 cases showed HER-2 protein overexpression(~)by IHC and 10 cases showed HER-2 gene amp1ification by SISH. The rate of HER-2 gene amp1ification was 66. 67%. 5 cases with 1ow HER-2 protein overexpression( +)and no case showed HER-2 gene amp1ification. Conclusions EGFR and HER-2 are high1y ex-pressed in co1orecta1 carcinoma. EGFR and HER-2 expression is connected to invasion and metastasis process of co1orecta1 carcinoma. The both may be synergy. The corre1ation between HER-2 protein overexpression(~)and HER-2 gene amp1ification is statisti-ca11y significant. Therefore,immunohistochemistry can be regarded as an initia1 screening method for HER-2 gene amp1ification,and then SISH testing shou1d be done as we11 to confirm the resu1t. It is usefu1 for mo1ecu1ar target therapy to detect HER-2 status in co1or-ecta1 carcinoma.