1.Study on the regulatory effects of mechano growth factor on soft tissue repair.
Can YU ; Yongqiang SHA ; Pan GUO ; Yimei CHEN ; Lucy Wanjiru NJUNGE ; Yonggang LU ; Li YANG
Journal of Biomedical Engineering 2015;32(1):235-239
Mechano growth factor (MGF) is an autocrine/paracrine factor and sensitive to mechanical stimulation. MGF can be highly expressed in various soft tissues under physical stimuli, biochemistry stimuli or in damaged situation. MGF may "compensate" the stress for tissue in the processing of tissue repair. MGF can effectively accelerate the repair of the soft tissue by promoting the proliferation, migration and differentiation of cells. This paper summarizes the MGF expressions in different soft tissues and their functions in soft tissue repair. The paper also discusses current problems and challenges in using MGF to repair the soft tissue.
Cell Differentiation
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Cell Proliferation
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Humans
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Insulin-Like Growth Factor I
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physiology
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Soft Tissue Injuries
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Wound Healing
2.Effects of total parenteral nutrition on drug metabolism gene expression in mice.
Christina FERRUCCI-DA SILVA ; Le ZHAN ; Jianliang SHEN ; Bo KONG ; Michael J CAMPBELL ; Naureen MEMON ; Thomas HEGYI ; Lucy LU ; Grace L GUO
Acta Pharmaceutica Sinica B 2020;10(1):153-158
Parenteral nutrition-associated liver disease (PNALD) is a liver dysfunction caused by various risk factors presented in patients receiving total parenteral nutrition (TPN). Omega-6 rich Intralipid® and omega-3 rich Omegaven® are two intravenous lipid emulsions used in TPN. TPN could affect the hepatic expression of genes in anti-oxidative stress, but it's unknown whether TPN affects genes in drug metabolism. In this study, either Intralipid®- or Omegaven®-based TPN was administered to mice and the expression of a cohort of genes involved in anti-oxidative stress or drug metabolism was analyzed, glutathione (GSH) levels were measured, and protein levels for two key drug metabolism genes were determined. Overall, the expression of most genes was downregulated by Intralipid®-based TPN ( and ). Omegaven® showed similar results as Intralipid® except for preserving the expression of and and increasing . Total GSH levels were decreased by Intralipid®, but increased by Omegaven®. CYP3A11 protein levels were increased by Omegaven®. In conclusion, TPN reduced the expression of many genes involved in anti-oxidative stress and drug metabolism in mice. However, Omegaven® preserved expression of , suggesting another beneficial effect of Omegaven® in protecting liver functions.