Objective To compare the evaluation effect ,clinical feasibility and influencing factors of two methods on comparative analyzing the blood glucose measurement of glucose meter and automatic analyzer .Methods 61 diabetic or non-diabetic patients were recruited in this study .All subjects were drawn 2 mL fasting venous blood using EDTA-K2 tubes following an 8~12 h over-night fast .One drop of venous whole blood was used to measure the blood glucose concentration (VBG)with the meter and the rest of blood was centrifuged for detecting the plasma glucose (VPG)by automatic analyzer (HITACHI-7600) .The capillary blood was taken by finger stick to test blood glucose(CBG)using the same meter within 5 min after the venous blood collection .Taking the VPG as the reference ,to calculate the respective bias of VBG and CBG .Correlation of VPG with VBG or CBG was analyzed .The clinical acceptance of glucose meter readings was evaluated by Parkes error grid analysis .Results Both VBG and CBG measured by Accu-Chek Performa glucose meter were highly correlated with VPG ,with correlation coefficient 0 .991 and 0 .989 respectively .All the measurements satisfactorily met the GB/T 19634-2005 criteria and all the results were clinically accepted .Conclusion To com-pare the results of blood glucose measured by glucose meter and automatic analyzer ,venous blood sample is recommended because of the same blood resource ,convenience of operation and possibility of making blood samples with extremely low or high glucose concentration .

Object To study the chemical constituents from Epimeredi indica and search for the natu-ral compounds with bioactivities. Methods Three compounds were isolated by a combination of RP-18and silica gel column chromatographies, and their structures were identified by spectral methods. ResultsA novel 2- (3-methoxy-4-hydroxy) phenyl-ethanol 1-O-a-L- [(1→3 )-rhamnopyranosyl- 6-feruloyl] gluco-side ( I ) along with known 2-(3, 4-dihydroxy) phenyl ethanol 1-O-?-L-[(1→3)-rhamnopyranoside-4-O-caffeoyl-] glucoside (verbascoside) ( Ⅱ ), and 2-(3, 4-dihydroxy) phenyl ethanol (1→1) (2→2)-[(1→3)-rhamnopyranoside-4-O-caffeoyl glucoside (oraposide) (Ⅲ) were isolated from E. indica. ConclusionCompound I is a new compound named as epimeredinoside A and compounds Ⅱ and Ⅲ are isolated fromthis plant for the first time.

AIM: To study the correlation between the release rate in vitro and the absorption in vivo of Shenshen sustained-release Capsule(SSRC phenylethanoid glycosides). METHODS: Release rate of SSRC in vitro was tested with D-800LS dissolution tester. 8 rats were randomly divided into two groups. One group was orally fed on SSRC. Plasma levels were determined by UV and the absorption fraction was calculated according to Wagner-Nelson's formula. RESULTS: The correlation coefficient between the release rate in vitro and the absorption fraction in vivo was 0.9654 . CONCLUSION: There is a significant correlation between release rate in vitro and absorption in vivo to SSRC.

Objective To investigate the association of cytotoxic T lymphocyte associated antigen 4(CTLA 4) gene polymorphism with type 1 diabetes in Chinese Han population.Methods The A/G phenotype at position 49 of the CTLA 4 gene exon 1 was determined by polymerase chain reaction restriction fragment length polymorphism(PCR RFLP)method in 33 typical type 1 diabetes patients,57 latent autoimmune diabetes in adults(LADA) patients and 84 healthy control subjects of Chinese Han.Results The frequency of the CTLA 4/G 49 phenotype was significantly higher in type 1 diabetes patients than in control subjects(55.6% vs 36.9%, respectively, P =0.0005),but there was no significant difference between typical type 1 DM and LADA groups. Neither the presence nor the absence of G 49 allele influenced the occurrence of islet autoantibody(ICA) and glutamate decarboxylase antibody(GADAb).Conclusion The polymorphism of CTLA 4 gene exon1 confers susceptibility to type 1 diabetes. This association is independent of ICA and GADAb.

Objective To evaluate the efficacy of intra-aortic balloon pump (IABP) on elderly patients with acute myocardial infarction (AMI) combined with cardiogenic shock (CS).Methods Among 62 elderly patients with AMI plus CS undergoing percutaneous coronary intervention (PCI),32 patients received IABP before PCI (IABP group) and 30 patients did not (control group).Diastolic blood press ure(DBP),mean arterial pressure (MAP),heart rate,cardiac index (CI),and ejective fraction before and 2 weeks after PCI in the two groups were observed.The short-and long-term therapeutic effects were compared between the two groups.Results The DBP,MAP and CI were higher and heart rate was lower in IABP group than in control group within 24 h after IABP [(64.4± 11.1) mmHg vs.(37.8±15.1) mmHg,(71.4±8.3) mmHg vs.(43.0±10.5) mmHg,(2.98± 0.33) L· min-1 · m-2 vs.(1.99±0.29) L· min-1 · m-2,(90.7±18.7) /min vs.(130.2±50.1)/min,t=7.97,11.83,12.51,4.16,all P=0.000].Two weeks after IABP,LVEF was significantly improved in IABP and control groups as compared with pre-IABP [(46.4±7.2)％ vs.(35.2± 7.2) ％,(39.1±6.8) ％ vs.(33.8±6.7) ％,both P＜0.01],and heart function was improved more significantly in IABP group than in the control group (t=3.91,P=0.000).Death tolls during hospitalization and after leaving hospital,and recurrence of AMI had no significant differences between the two groups (2 cases vs.4 cases,3 cases vs.8 cases,6 cases vs.10 cases,x2 =0.89,3.17,1.72,P=0.346,0.075,0.190).Conclusions IABP can improve the cardiac function in elderly AMI patients with CS after PCI.

Objective To compare the endocrine and metabolic parameters between patients suffering from polycystic ovary syndrome (PCOS) with or without subclinical hypothyroidism.Methods One hundred and fifty-six PCOS patients and 47 healthy controls were enrolled.Height,weight,waist circumference,and hip circumference were measured and recorded.Fasting blood samples were collected for measuring luteinizing hormone (LH),follicular stimulating hormone (FSH),total testosterone,prolactin,estrogen,sex hormone binding globulin (SHBG),thyroidstimulating hormone (TSH),free triiodothyronine (FT3),free thyroxine (FT4),blood glucose,serum insulin,triglycerides,total cholesterol,low-density lipoprotein cholesterol(LDL-C),and high-density lipoprotein cholesterol (HDL-C).Body mass index,waist-to-hip ratio,LH-to-FSH ratio,and free androgen index (FAI) were calculated.Homeostasis model assessment of insulin resistance (HOMA-IR) and β cell function (HOMA-β) were assessed.All patients underwent ultrasound for diagnosing polycystic ovary.All the patients were subdivided into two groups in terms of TSH value:subclinical hypothyroidism group with TSH level ≥4.2 μIU/ml and non-subclinical hypothyroidism group with TSH level＜4.2 μIU/ml.The differences in endocrine and metabolic parameters were compared between two subgroups.Results Compared with healthy controls,the PCOS patients had significantly higher body mass index,waist-to-hip ratio,LH,LH-to-FSH ratio,total testosterone,FAI,TSH,and lower SHBG (all P＜0.05).The other indexes were not different between two groups (P＞0.05).24.4％ PCOS patients were diagnosed as cases of subclinical hypothyroidism.TSH,prolactin,and triglycerides levels were higher in PCOS patients with subclinical hypothyroidism than those without (P＜0.01).Whereas estrogen,FT3,FT4 were significantly lower(P＜0.01).The differences of other parameters were not significant between two groups(P＞0.05).The results of correlation analysis and TSH quartiles showed no significant linear correlation between TSH and sex hormones,lipids,insulin resistance indices (P＞0.05).Conclusions The incidence of subclinical hypothyroidism in all PCOS patients is 24.4％.Prolactin and triglycerides levels were higher in PCOS patients with subclinical hypothyroidism than those without.Whereas estrogen,FT3,FT4 were significantly lower.

BACKGROUND:Receptor-interacting protein 140-knockout mice are lean and resistant to high fat diet-induced obesity due to an increase in mitochondrial biogenesis, fatty acid oxidation and oxidative phosphorylation.
OBJECTIVE:To study the expression level of receptor-interacting protein 140 mRNA in adipose tissue of high fat diet-induced obese mice and the correlation with insulin resistance.
METHODS:Male C57BL/6J mice were randomly divided into control group and high fat diet group, and fed for 14 weeks separately. The mice of the two groups were weighed. Mice in high fat diet group whose body weight were 20%higher than average weight of control mice were selected as obese mice.
RESULTS AND CONCLUSION:A total of 12 mice in high fat diet group were recruited into the obesity group. The levels of triglycerides, total cholesterol, fast blood glucose and fast insulin in obesity group were significantly higher than those of control group (P<0.05 or P<0.01). The expression of receptor-interacting protein 140 mRNA in adipose was significantly higher in obesity group compared with the controls (P<0.05). Moreover, the expression of receptor-interacting protein 140 mRNA in adipose was positively correlated with triglycerides levels and insulin resistance index (r=0.526, P<0.05;r=0.465, P<0.05), but negatively associated with the levels of total cholesterol, fast blood glucose and fast insulin (P>0.05).

AIM: To study Cordyceps sinesis and its cultured mycelia by HPLC.METHODS: An HPLC established for the fingerprints of cordyceps sinensis and its cultured mycelia was performed on an Agilent C18(4.6 mm ? 25 mm,5 ?m)as column and acetonitrile-0.1% phosphoric acid solution in gradient as mobile phase.The flow rate was 0.5 mL/min,and the detection wavelength was set at 260 nm.RESULTS: There were 6 common peaks in HPLC fingerprint of Cordyceps corensis and its cultured mycelia,three of which were determined as uracil,adenosine,cordycepin.CONCLUSION: The method can be used for the study of fingerprints of cultured mycelia.

Objective To investigate the association of cytotoxic T lymphocyte associated antigen 4 (CTLA 4) gene polymorphism with type 1diabetesmellitusandautoimmunethyroiddiseases. Methods The A/G phenotype at position 49 of the CTLA 4 gene exon 1 was determined by PCR RFLP method in 33 classic type 1 diabetes patients, 57 latent autoimmune diabetes in adults (LADA) patients, 122 autoimmune thyroid disease patients and 84 healthy control subjects of Chinese Han. Results The frequency of the CTLA 4 G phenotype was significantly higherintype1diabetespatientsthanthatincontrol subjects (55.6% vs 36.9%, P=0.0005). Neither the presence nor the absence of G allele influenced the occurrence of islet autoantibody (ICA) and glutamate decarboxylase antibody (GADA). The strong association of the CTLA 4 G allele with AITDs was showed in our study (66.4% vs 36.9%, P

To investigate the prognosis relevant factors of endometrial carcinoma. Clinical pathological materials of 68 cases of endometrial carcinoma were analyzed. The 5 year survival rate of ≥40 years old women with endometrial carcinoma was slightly less than that of the younger women, while menopause showed no influence on the survival rate .The 5 year survival rate of patients with term parity ≥2 was 92 7%( P 0 05). Therefore, surgical method ,clinical staging ,invasion depth of uterine muscle and number of term parity are the important factors which influence the prognosis of endometrial carcinoma.