Twenty Rota virus strains were isolated from 20 stool specimens derived from diarrhea children in Khanh Hoa province. After 3 times of passaged culturations on MAI04 cells, there were only 2 human Rota virus strains growing well in the medium of trypsin concentration at 5 mg/ml and 4 human Rotavirus strains multiflying well in the medium of trypsin concentration at 10 mg/ml.
Rotavirus ; Diarrhea, Child ; Child

Rota virus of the type of G4P6, 8 (VNHR 203) is one of endemic strains in Vietnam causing acute diarrhea in under- five -year -old children. In the year 2000 this strain was isolated from fecal sample of a 9 month boy with acute diarrhoae admitted to St Paul hospital. Viruses were carelully selected and underwent 10 consecutive transferred culturing on Rhesus (MA104) monkey kidney cell medium, then 20 times of assimilating on Macacca mulatta monkey primitive kidney cells.They had got high safety and efficacy in the trial on Macacca mulatta aged 12-24 months
Macaca mulatta ; Haplorhini ; Rotavirus ; Diarrhea ; child

41 rotavirus strains that were collected at Hai Phong Children Hospital were determined G, P genotypes by Rt-PCR method. Of these, G9 was the most common (75.62%), followed by G4 (12.19%), G1 (4.87%), and untyped (9.75%). For P genotype, P8 (46.34%), P6 (43.90%) were the most common. The common phenotype of rotavirus strains in Hai Phong Children Hospital were G9P8 (43.90%), G9P6 (21.95%), and G4P6 (12.20%)
Gastroenteritis ; Rotavirus ; child ; diagnosis ; epidemiology

Using rotavirus cultural methods isolated from 2001019203 strain in cellular fluid containing trypsine and rotavirus cloning method in stool samples of diarrhea children at the age of 9 months old, treated in St. Paul's Hospital in 2001. The results: 2001019203 strain was type G4, which was developed in MA 104 cellular fluids. 6/11 clones of the second cloning rotavirus were adaptable and development in cellular fluid MA104 were selected, in which only 3 clones were both adaptable and development as A-1, C-1* and E-3. All 6 adaptable colony were selected into further study
Rotavirus ; Macaca mulatta ; kidney ; cells

10 strains of rotavirus C-1P6 causing acute diarrhoea in under 5- year - old - children were cultured on African Rhesus monkey kidney cells. Only 4 strains were adapted in the mixture of monkey MA104 kid cells. Results showed that in the most favorable medium, not all strains of virus could be adapted and grown properly. Rotaviruses of A group is the main agents causing acute diarrhoea in children in winter
Diarrhea ; Rotavirus ; Kidney ; child ;

Four line of cells Hep2, Vero, MA104 and JVKT are used for quality control and a line of monkey kidney cells used for production of poliomyelitis vaccine. Contaminated mycoplasma was tested by culture method and immu-mark mycotest. Same results were given: 50% of Hep 2 cells and 66.6% of MA104 were contaminated with mycoplasma. There is not any controlled lot of JVKT. Vero and monkey kidney which was contaminated
Poliomyelitis ; Mycoplasma ; Quality Control ; Vaccines

Rotavirus strains isolated from 3 acute diarrhea calves at Ba Vi cattle farm were cultured and firstly cloned in African green monkey kidney (Vero) cells and African Rhesus monkey kidney (MA104) cells. After 3 times of passage, the calf rotavirus strains were secondly cloned and all of them were adapted in primary kidney cells of Macaca mulatta
Rotavirus ; Cell Culture Techniques ; Diarrhea

By RT-PCR biomolecular technique on ARN isolated from 30% fecal mixture of 41 samples of under five years old children with acute diarrhoea due to rotavirus collected at Khanh Hoa Pediatric Hospital, the year 2002, 4 genotypes (G9, G1, G2 and G4), 3 P genotypes (P4, P6, P8) and 2 common phenotypes G1P8 and G2P4 were determined. While 9.75% of G gene and 21,95% of P gene were not determined yet
Diarrhea ; Rotavirus ; Child

2455 fecal samples of acute diarrhoea children collected from National Institute of Pediatries, Hai Phong Pediatric hospital, Khanh Hoa Pediatric hospital, HoChiMinh city Pediatric hospitals No1 and No2 in the year 2002. By EIA assay, in 1042 samples (42.62%) Rotavirus was determined as positive. The virus existed around all the year, concentrated in Jan, Feb, March, Apr and July, affected commonly the children aged 3-35 months. From 154 genus of rotavirus, G type, G9 genus is the most common (90 genera). In addition, G2 (21 genera), G1 (19 genera), G4 (12 genera) and mixing types and unidentified types (12 genera) were also detected
Rotavirus ; epidemiology ; Diarrhea

Collecting stools sample from 232 children with acute diarrhoea, who treated in hospitals. Using diagnose immune enzyme EIA bio-product branch defined that reason of the disease is rotavirus. In 10 typies of rota G1P8 that cause of disease in Vietnam only have suitable and develop 4 typies in renal cell combination of Africa Rhesus monkey are G1P8 that signed 2001019423-11, 2001019427-15, 2001019428-17, 2001019432-20
Rotavirus ; Diarrhea ; Child