Objective To understand the genotoxicity of organic extracts (OEs) of fishpond water containing sewage.Methods Male Kunming mice were randomly divided into different groups according to the body weight,6 in each group.The OEs were administrated to the mice through peritoneal injection at the doses of 25.00 L/kg,12.50 L/kg and 6.25 L/kg respectively for 3 consecutive days.The negative and positive control groups were treated with DMSO and cyclophosphamide (CP) respectively.The DNA damage was tested by using un-fluorescent staining comet assay.Results The tailed-cells rates and tail length in each OEs treated group were significantly higher than those in the negative control group (P

Objective To study the mutagenicity of fish-raising water containing effluent. Methods The niuta-genieities of water samples from waste water canal, the fish-raising water(fish-pond water) treated by presedimenta-tion in oxidation tank were detected using micronucleus test of vicia faba root tip cells. The micronucleus test of peripheral red blood cells was carried out among fish raised in water containing effluent. The water samples and fish collected from reservoir as controls. Results The micronucleus test of vicia faba root tip cells showed that the micronucleus rates in treated fish-pond water and waste water were significantly higher than those of negative control group (distilled water) (P 0.05) . The frequencies of micronucleus in peripheral red blood cells of fish raised in treated fish-pond water were significantly higher than those of fish raised in reservior water (P

OBJECTIVE:To work out the optimal inventory levels in zero inventory management mode through model predic-tion and control strategy,by using the inventory upper & lower limits settings generally available in the information management system of health care institutions. METHODS:Multi-varieties joint ordering modelwas constructed by referring to operations management,time series analysis and quantitative approach to decision-making,that is,to make a prediction of upper&lower lim-its on medicine inventory based on historical data and applicable mathematical models(fixed order interval model and re-order mod-el,i.e. FOI and ROP),and compared with real results;based on above,specific medicine procurement and inventory control strat-egies would be developed and an evaluation of the application effects would be made. RESULTS:The error test and reproducibility test exhibited that the out-of-stock ratio remained under 3.36%,of which 71.24% could be effectively alarmed;under computer simulation and practical operation,the instant replenishment rate reduced by 9.33% and 13.03%,OOS ratio down by 11.11% and 27.45%,and average daily inventory turnover rate up by 30.19% and 15.85% respectively,all showing remarkable improvements compared to before the implementation of the mode. CONCLUSIONS:This model is of favorable accuracy and operability,there-fore it can lay foundation for rational and well-founded decisions in medicine procurement and inventory control in zero inventory management mode.

OBJECTIVE To study the meaning of detecting the antibody of Salmonella typhi by gel technique(GEL). METHODS Minigel column technique(MGCT) and Widal reaction(WR) were used to detect the antibody among 32 suspicious typhoid and paratyphoid patients. RESULTS Among them,the titers of various kinds of antibody of S.typhi "O","H","?","?",and "?" with GEL were 1-2 orders higher than with WR.There was a significant difference in statistics. CONCLUSIONS It is convenient and fast to detected the antibody of S.typhi.And it can give important reference data for the clinic.

Objective To observe the surface morphology of dentin irradiated by Er,Cr∶YSGG laser under anhydrous conditions and discuss the possible clinical applications of the laser.Methods 11 freshly extracted specimens (approximately 1.5 mm thick) taken from the first premolars were selected.One was regarded as the control group without any treatment and the remaining ten were divided into 5 groups whose dentin surface was irradiated by Er,Cr∶ YSGG laser with different energy (1,2,3,4,6 W) under anhydrous condition.The specimens were routinely prepared for electron microscopic scanning observation.Results As the output power increased with Er,Cr∶YSGG laser irradiation,the dentin surface was observed of the constriction of tubular lumen,and part of the tubules closed.The dentin surfaces with 3 ～6 W laser irradiation were observed thermal injury alters such as micro crack and carbonization.Conclusion 1 ～2 W anhydrous Er,Cr:YSGG laser irradiation play a more ideal and safe constriction and sealing role to dentinal tubules,and the laser is expected to be used for the treatment of dentin hypersensitivity.

Objective To evaluate the mutagenicity of mercury, cadmium, arsenic, chromium(Ⅵ)and lead in water on Vicia faba root tip cells. Methods The micronucleus assay of Vicia faba root tip cells were conducted to determine micronucleus rate of Vicia faba root tip cells induced by mercury (0.000 5-1.0 mg/L), cadmium (0.005-1.0 mg/L), arsenic(0.05-2.0 mg/L), chromium (Ⅵ) (0.05-2.0 mg/L) and lead (0.05-3.0 mg/L ) ,and pollution indexes were calculated. Results When the concentration of mercury was ≥ 0.5 mg/L, cadmium ≥ 0.1 mg/L, arsenic ≥ 0.5 mg/L and chromium(Ⅵ)≥ 2.0 mg/L in the water, the micronucleus rate were significantly higher compared with the negative control group, respectively(P

Objective: To discuss the applied effect of high frequency repetitive transcranial magnetic stimulation combined with cognitive intervention for stroke patients with aphasia. Methods: 84 stroke patients with aphasia were divided into observation group (42 cases)and control group (42 cases). Patients of control group were treated by routine intervention of language training, while patients of observation group were treated by high frequency repetitive transcranial magnetic stimulation combined with cognitive intervention. A series of evaluated indicators, such as the language function score of Boston diagnostic aphasia examination(BDAE), the anxiety symptom score of Hamilton anxiety scale(HAMA), the quality of life and degree of satisfaction, were adopted to compare the post-treatment effect for the two groups. Results: After the treatment, there were statistical significance in the difference of understanding, retelling ability, reading ability and writing ability about BDAE scores between the two groups (t=3.572, t=4.482, t=6.238, t=3.013; P<0.05), and the HAMA scores of observation group was significant higher than those of control group (t=3.472, P<0.05). Besides, through different interventions, the series of indicators about quality of life, such as physical function, mental function, social function and material function, of observation group were higher than those of control group (t=4.513, t=3.923, t=4.473, t=4.033; P<0.05). And the satisfaction of observation group was significant higher than that of control group (x2=5.233, P<0.05). Conclusion: The clinical effect of high frequency repetitive transcranial magnetic stimulation combined with cognitive intervention for stroke patients with aphasia is favorable. And this method can increase quality of life and satisfaction for patients, and can extremely reduce medical dispute and improve the therapeutic level of hospital for stroke patients with aphasia.

ve To assess the cytogenetic toxicological safety of fish raised in water containing effluent. Methods The mutagenecity of organic extracts of uncooked meat specimens of fish raised in fishpond water mixed with sewage water taken from South and North Sewer Drainage Canals of Tianjin was detected by mice bone marrow micronucleus test. Results The organic extracts of uncooked fish meat specimens showed positive result for all of the 3 observed groups of fish raised in water of fish ponds with sewage, and negative result for fish raised in clean fishpond water in micronucleus test. Conclusion The results indicated that some kinds of organic mutagenic compounds ex-isted in the uncooked meat of fish raised in fishpond water containing effluent, which might have some potentially hazardous effects on human health through the food chains.

As a kind of source wide,low inherent immnunogenicity multipotent stromal cells,mesenchymal stem cells (MSCs) have been used to cell-based therapeutic researches of immunodeficient deseases or other severe clinical disorders.Despite low levels of engraftment in the injured lung,numerous preclinical trials indicate that mesenchymal stem cells can promote damaged tissue repair constructed by secreting paracrine factors to regulate the host immunity,inhibit inflammation and restore epithelial integrity.Acute lung injury(ALI) is a serious organ dysfunction occurred earliest and the most commonly affected organ.Currently there is no effective medicine therapy and the mortality remains high.Researches demonstrate MSCs can reduce alveolar edema,improve lung function and reduce motality.So understanding of the mechanism will be conductive to the MSCs from experimental stage into clinical application.This issue will put a brief overview pointed on the mechanism of mesenchymal stem cells in repairing the injured lung.

Objective To evaluate treatment outcomes and quality of life (QOL) in glioma patients treated with postoperative intensity-modulated radiotherapy (IMRT), and to explore the possible clinical factors of affecting QOL. Methods From 2007 to 2009, 37 patients with low or high grade glioma were analyzed retrospectively. All patients were operated by tumor resection below microscopy. IMRT began at 2-4 week postoperstion with 2.0 Gy/fractior, 5 fractions/week and to shrink portal and to add dose to 50-60 Gy/25-30 fractions after 40-50 Gy. The gross tumor volume (GTV) was defined as preoperation T2WI MRI high sign area and postoperation tumor cavity for low grade glioma, and with preoperation T1WI MRI enhanced abnormity area and postoperation tumor cavity for high grade glioma. The clinical target volume ( CTV ) was defined as GTV with a margin of 1.5 cm for low grade glioma and a margin of 2.5 cm for high grade ghoma, the planning target volume (PTV) with CTV plus 0.4 cm margin for setup errors according to the European Organization for Research and Treatment of Cancer ( EORTC ).The treatment outcomes and QOL were assessed. Results The half-year and one-year survival rates for all the patients were 100% and 79.2%, respectively. The median progression-free survival time was 10 months. The main side-responses after postoperative IMRT were fatigue and mild memory decline or cognitive disabilities, which were radiation dose-dependent. Conclusions Postoperative IMRT is an effective and safe modality of therapy for glioma patients.