1.Cloning and analysis of STR gene and its promoter from Uncaria
Hao ZHOU ; Xing-xing LU ; Wen-wen AO ; Hai-min LIAO ; Ming-sheng ZHANG ; Wei QIANG
Acta Pharmaceutica Sinica 2022;57(5):1526-1536
On the basis of the
2.Resistant analysis and cultivation results of 3 160 blood specimen.
Jin-xing ZHANG ; Dan-qian LU ; Jian-wen YI
Journal of Central South University(Medical Sciences) 2005;30(1):121-122
Adolescent
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Adult
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Aged
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Aged, 80 and over
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Azithromycin
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pharmacology
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Bacteremia
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microbiology
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Child
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Child, Preschool
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Culture Media
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Drug Resistance, Bacterial
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Drug Resistance, Multiple, Bacterial
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Escherichia coli
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drug effects
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isolation & purification
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Female
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Humans
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Infant
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Infant, Newborn
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Male
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Methicillin Resistance
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Middle Aged
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Penicillin G
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pharmacology
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Salmonella paratyphi A
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drug effects
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isolation & purification
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Staphylococcus aureus
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drug effects
;
isolation & purification
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Staphylococcus epidermidis
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drug effects
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isolation & purification
3.Students' leading functions in the anatomy teaching reform
Xing-lu WANG ; Wen-jing TANG ; Hai-xia HUANG ; Yu-xing SUN ; Shan-quan SUN ;
Chinese Journal of Medical Education Research 2011;10(9):1069-1071
As the main part of the teaching activities,students play an important role in the teaching reform.The students were trained through 3 pathways,“Extending teaching activities from the classroom to the outside”,“Development from basic to clinical knoledge” and “Culturing students' innovative consciousness”,so as to allow them to give full play in teaching reform,to enhance their ability of practice and learning by themselves,to culture their innovative consciousness and to develop students' leading functions in the anatomy teaching reform.
4.Screening of target genes in esophageal squamous cell cancer in Kazakh by oligonucleotide microarray
Xiaomei LU ; Hui PFNG ; Zan LIU ; Manshu SONG ; Xing WANG ; Tuerxun AERZIGULI ; Xiaheding YILIYAER ; Hao WEN
Chinese Journal of Digestive Surgery 2008;7(5):372-374
Objective To screen the differentially expressed genes in esophageal squamous cell cancer (ESCC) and normal tissue of esophageal mucosa in Kazakh. Methods RNA was extracted from the ESCC sections in Kazakh patients, and was amplified to obtain cRNA. The gene expression profiles in ESCC and normal tissue of esophageal mucosa were detected by HG-U133 Plus 2.0 gene chip. The results were analyzed by bioinfor-matics. Results One hundred and seventy differentially expressed genes in ESCC and normal tissue of esophageal mucosa were found, with a difference of more than 10 times in expression levels. Of the 170 genes, 39 were up-regulated (signal log ratio > 3 ) and 131 down-regulated (signal log ratio < - 3). These factors such as cell cycle regulation, apoptosis, cytoskeleton; extracellular matrix, intracellular signal transduction, protein translation and synthesis, and immunological functions were correlated with the genes with abnormal expression. Conclusion The use of oligonucleotide microarray accurately and efficiently screen the 170 target genes in ESCC in Kazakh. It is suggested that these genes may be related to the carcinogenesis and development of ESCC in Kazakh.
5.Diagnostic and therapeutic values of capsule endoscopy in Crohn's disease.
Acta Academiae Medicinae Sinicae 2008;30(2):175-177
OBJECTIVETo explore the diagnostic and therapeutic values of the capsule endoscopy (CE) in Crohn's disease (CD).
METHODSThe clinical data of 14 patients diagnosed by CE were retrospectively analyzed. The clinical features, CE findings, and medical management were evaluated.
RESULTThe severity of CD diagnosed by CE was consistent with the clinical features.
CONCLUSIONThe CE findings are important indicators in CD diagnosis and may facilitate clinical decision making.
Adolescent ; Adult ; Anti-Inflammatory Agents ; therapeutic use ; Capsule Endoscopy ; methods ; Crohn Disease ; diagnosis ; drug therapy ; Female ; Humans ; Male ; Middle Aged ; Retrospective Studies ; Young Adult
6.Study on the pharmacodynamic material basis of Xiangju Preparations in the treatment of rhinitis and sinusitis based on network pharmacology
Sai-yu LI ; Yi-wen ZHANG ; Pan-pan YANG ; Xin-ran WANG ; Lu-wen XING ; Qing LI
Acta Pharmaceutica Sinica 2022;57(8):2471-2483
In order to clarify the pharmacodynamic substances and mechanism of Xiangju Preparations (Xiangju Tablets, Xiangju Drops) in the treatment of rhinitis and sinusitis, the multi-level network integration analysis of "ingredients-targets-pathways" was conducted. 137 chemical constituents were identified in Xiangju Preparations by high pressure liquid chromatography-quadrupole-time of flight mass spectrometry (HPLC-QTOF/MS) for the first time. Network pharmacology analysis was performed on 59 potential active components. The results of network pharmacology analysis demonstrated that the medicinal ingredients in Xiangju Preparations included caffeic acid, senkyunolide F, rosmarinic acid, ligustilide, prim-
7.Tendon-regulating and bone-setting manipulation plus endurance resistance exercises for female with chronic neck pain
Wen-Di ZHANG ; Zhao-Hui CHEN ; Hui ZHANG ; Meng-Xing LI ; Lu-Lu CHENG ; Kai-Wei ZHANG
Journal of Acupuncture and Tuina Science 2021;19(5):389-397
Objective: To observe the clinical efficacy of tendon-regulating and bone-setting manipulation combined with endurance resistance exercises in treating female with chronic neck pain, and explore the mechanism. Methods: A total of 57 female patients with chronic neck pain who met the inclusion criteria were randomly divided into a manipulation group (29 cases) and a medium-frequency electrotherapy group (28 cases). Patients in both groups received the same endurance exercise therapy, while those in the manipulation group received additional tendon-regulating and bone-setting manipulation, and those in the medium-frequency electrotherapy group received additional medium-frequency electrotherapy. Both groups were treated for 5 weeks. Before and after treatment, the neck function of patients was evaluated by visual analog scale (VAS), Analgesy-Meter, Northwick Park questionnaire (NPQ), root mean square (RMS) and median frequency (MF) of surface electromyography of sternocleidomastoid muscle and posterior cervical extensor muscle, and the patients were followed up at a month after treatment. Results: All patients completed the treatment and were followed up. Compared with the same group before treatment, the VAS scores of both groups decreased, the tenderness values increased, the RMS and MF values increased, and the NPQ scores decreased after treatment (all P<0.05). The improvement of manipulation group was more notable than that of medium-frequency electrotherapy group (all P<0.05). At one-month follow-up, the VAS and NPQ scores of the manipulation group were lower than those before and after treatment, and the VAS and NPQ scores of the medium-frequency electrotherapy group were only lower than those before treatment; the two scores of the manipulation group were lower than those of the medium-frequency electrotherapy group (both P<0.05). Conclusion: Tendon-regulating and bone-setting manipulation combined with endurance resistance exercises can relieve neck pain and cervical dysfunction in female patients with chronic neck pain. The efficacy of this method is more durable and better than that of medium-frequency electrotherapy combined with endurance exercises.
8.Effect of the rolB gene on phenotypic development and tropane alkaloids biosynthesis in Atropa belladonna
Wen-wen AO ; Ai-juan TAN ; Bing-nan MA ; De-hui MU ; Xing-xing LU ; Hang PAN ; Ming-sheng ZHANG ; Wei QIANG
Acta Pharmaceutica Sinica 2023;57(6):1705-1712
The
9.Establishment of serological detection methods of 3 types of human herpesvirus 8 specific-antigens and complex antigens
Xing WANG ; Fang-Ping HE ; Xiao-Mei LU ; Shu-Jun ZHAO ; Ren-Yong LIN ; Bin HE ; Hao WEN ;
Chinese Journal of Infectious Diseases 2007;0(09):-
Objective To establish serological detection methods of human herpesvirus 8 (HHV-8).Methnds Three potent antigenic fusion proteins.K8.1,ORF65 and ORF73 C of HHV- 8 were synthesized using E.coli system.The sera were detected using lhese antigenic proteins.The positive sera were from 12 patients with Kaposi's sarcoma and 32 patients with acquired immunodeficiency syndrome-related Kaposi's sarcoma.The negative sera were from 20 patients with cutaneous tumors and children under 15 years old.Western blot and enzyme-linked immunosorbent assay (EI.ISA)were employed to determine the immunogenicity of each recombinant protein and the sensitivity and specificity of ELISA using the complex antigens.Results Three types highly purified HHV 8 specific recombinant pro teins with potent antigenicity were successfully synthesized.The sensitivity of ELISA using the above complex antigens was significantly higher than traditional immuno-flurescent assay (IFA)detecting the positive and negative sera,whieh were 81.8%,34.4%,respectively.And the specificity of ELISA was 97.9%.Conclusion K8.1,ORF65 and ORE73 C are good candidate antigens for establishing HHV-8 serological detection methods,which have better sensitivity and specificity.
10.Molecular cloning, sequencing and function of extracellular signal regulated kinase of Echinococcus granulosus
Guodong Lü ; Jing JI ; Junhua WANG ; Liang LI ; Hongli WANG ; Xiaomei LU ; Xing WANG ; Hao WEN ; Renyong LIN
Chinese Journal of Infectious Diseases 2010;28(7):402-407
Objective To perform molecular cloning and sequencing, bioinformatics analysis,protein expression and function of extracellular signal regulated kinase (EgERK1) of Echinococcus granulosus in Xinjiang. Methods The specific primers of EgERK1 were designed and total RNA was extracted from Echinococcus granulosus in Xinjiang. EgERK1 gene was amplified by reverse transcription-polymerase chain reaction (RT-PCR) and prokaryotic expression plasmid pET28a-EgERK1 was constructed and sequenced. The sequences were analyzed by DNA sequencing and bioinformatics technology. The recombinant EgERK1 protein was induced and expressed. The biological function was detected using sodium dodecyl sulfate polyacrylamide gel electropheresis and Western blot. Results The sequence of RT-PCR product was 1125 bp, encoding 374 amino acids with isoelectric point of 6.34.This gene was a new ERK-homologues gene indicated by BLAST, named EgERK1(EU701008).Homology comparisons indicated that the homology of EgERK1 and EmMPK1from Echinococcus multilocularis was 95.45%, and was 43.04%-61.88% to ERK from Caenorhabditis elegans, S. cerevisiae, D. melanogaster and human. Phylogenetic analysis showed that EgERK1 clustered with EmMPK1. Bioinformatics analysis predicted that EgERK1 contained a highly conserved T-X-Y motif and activation loop segment of ERK-like kinase.Western blot results showed the EgERK1 recombinant protein could reacted specifically with anti-human ERK monoclonal antibody. Conclusion A new EgERK1 gene of Echinococcus granulosus is successfully cloned and its recombinant protein could reacted specifically with ERK1/2 antibody, which provides the basis for further study of EgERK1 function in the host-parasite interaction.