Objective: To compare the therapeutic effects and adverse reaction of late-course three-dimensional conformal concurrent chemoradiotherapy (3DCRT) and conventional concurrent chemoradiotherapy on stage III non-small cell lung carcinoma. Methods: This study retrospectively analyzed the clinical data from 87 cases with stage III non small cell lung carcinoma. The patients were randomly divided into 3DCRT group (group A, n = 31) and conventional concurrent chemoradiotherapy group (group B, n = 56). Group A was given docetaxel 40 mg once a week for 4-6 times and concurrent radiotherapy at 40 Gy followed by 3DCRT at a total dose of 70-74 Gy. Two weeks after radiotherapy, patients received carboplatin at 300 mg/m2 and docetaxel at 75 mg/m2. Four weeks were regarded as one cycle and the chemotherapy lasted for 4 weeks. Group B were treated with conventional radiotherapy at a total dose of 60-64 Gy. The chemotherapy regimen was the same with group A. Results: This study followed up 87 cases of NSCLC patients for 24 to 36 months. The complete and partial response (CR + PR) rate of group A and group B were 80.65% and 76.56%, respectively. one-and 2-year survival rates were 51.61% and 35.48% for group A, and 35.71% and 17.86% for group B. The difference was not significant between the two groups. The incidences of acute irradiation esophagitis, acute radiation pneumonitis, and myelosuppression were 58.06%, 6.45%, and 54.83% for group A and 79.64%, 16.07%, 85.71% for group B. The difference was not statistically significant. Conclusion: The therapeutic effects of 3DCRT are better than those of conventional concurrent chemoradiotherapy, but the incidence rates of adverse reactions do not increased.

BACKGROUND:Obturation with warm gutta-percha can achieve perfect three-dimensional root canal obturation effect. Due to specific structure of oval canal, particularly in the bypass preparation process, excessive cutting of local tooth tissue may lead to tooth tissue thinning and irregular shape. The separation equipment is the metal with good heat conduction, so the heat caused by the bypassing warm gutta-percha obturation would burn periodontal tissue remains controversial. OBJECTIVE:To investigate the changes of root surface temperature produced by two bypassing warm gutta-percha obturation for the separation instrument in oval canalin vitro by using infrared thermography. METHODS:Forty mandibular first premolars were harvested from oval canals, and were separated using stainless steel 15# K file at apical 3 mm, with the length of 3 mm. The root canal bypassing was prepared using TF nickel-titanium file under operating microscope. The teeth were randomly divided into two groups, with 20 teeth in each group, and were obturated with the Thermafil warm gutter-percha and E&Q plus warm gutta-percha vertical compaction, respectively. An infrared thermoviewer was used to measure the external root surface temperature before and after the obturation. RESULTS AND CONCLUSION:Two warm gutta-percha obturation methods used for separating instruments bypassing in the oval root canal, had both caused the rise of root surface temperature, which was less than 10℃. In the Thermafil group, the temperature changes were 3.2-8.1℃ and the average change was 4.97℃. In the vertical condensation group, the temperature changes were 5.5-9.8℃ and the average change was 7.35℃. There were significant differences in the change of root surface temperature between the two groups (P < 0.05). Thermafil warm gutter-percha obturation would increase the root surface temperature, but cannot damage periodontal tissues. It is more secure than warm gutta-percha vertical condensation.

Objective To study the antibiotic resistance mediated by the mar mechanism in clinical isolated Escherichia coli. Methods To analyse the antibiotic resistance pattern and the out membrane protein spectrum of clinical isolated E. coli . The plasmid pNJR3 2 carrying the wild type gyr A gene was transferred into clinical multiple resistant strain LC 1. The resistant pattern and plasmid pattern of LC 1 before and after plasmid transferring were compared. The mar OR of clinical strain LC 1 was cloned and sequenced through PCR. The nucleotides of mar gene were compared with the wild type issued by GenBank. Results Of all the 49 clinical strains , six strains express Mrp5, a mar specific out membrane protein. No changes were detected on susceptibility to fluoroquinolone of clinical strain LC 1 after pNJR3 2 transferred suggesting that gyr A was not the main reason contributing to fluoroquinolone resistance. Sequence analysis showed three mutant spots in mar OR of clinical strain LC 1. Conclusion There does exist mar mechanism in clinical isolated Escherichia coli resistant to fluoroquinolone. The mutation in mar OR may contribute to the mar phenotype of LC 1.

Objective To analyze retrospectively the HRCT signs in the patients with invasive pulmonary aspergillosis and evaluate the value of HRCT in the diagnosis of invasive pulmonary aspergillosis.Methods The cilinical and HRCT images of 30 cases with invasive pulmonary aspergillosis diagnosed by fiber bronchoscopy, CT guided biopsy or sputum culture were collected.HRCT images were analyzed and the HRCT signs were summarized by two experienced chest imaging radiologists.Results 19 patients had a variety of CT signs, the sign of tree in bud was seen in 8 cases, bronchial stenosis 6 cases, bronchiectasis 8 cases, ground-glass opacity 8 cases, acinic nodules 10 cases, nodular lesions 12 cases, acinar nodules with halo sign 4 cases, nodules with halo sign 9 cases, cavity 10 cases.11 cases only had a single CT sign, the sign of tree in bud was seen in 2 cases, bronchiectasis 2 cases, ground-glass opacity 1 case, acinar nodules 2 cases, nodules with halo sign 2 cases, cavity 2 cases.The occurrence rates of various signs in 30 cases were as follows, the sign of tree in bud was 33.3%, bronchial stenosis 20%, bronchiectasis 33.3%, ground-glass opacity 30%, acinar nodule 40%, nodular lesion 46.6%, halo sign 53.3%,cavity 40%.Conclusion The main HRCT signs in the patients with invasive pulmonary aspergillosis includes tree in bud, bronchial stenosis, bronchiectasis, ground-glass opacity, acinar nodules, nodal lesions,pulmonary cavity and halo sign.The signs of bronchiectasis with tree in bud sign, acinar nodule and halo sign in the HRCT images are highly specific in the diagnosis of invasive pulmonary aspergillosis.

BACKGROUND:Due to the complexity and irregularity of bypass obturation of oval root canal and the particular stress of the post and core to the tooth, we have not yet found a reasonable post crown for dental restoration after bypass obturation of the oval root canal.
OBJECTIVE:To compare the flexural capacity of the three kinds of post-and-core repair systems (cobalt-chromium cast post and core, zirconium oxide post and core, and CAD/CAM-fabricated glass fiber post and core) after bypass obturation of the oval root canal warm gutta.
METHODS: Ninety mandibular first premolars were selected for bypass obturation of the root canal with Obtura II & System B, and then randomized into three groups that were respectively restored with cobalt-chromium cast post and core, zirconium oxide post and core and CAD/CAM-fabricated glass fiber post and core. After that, cobalt-chromium metal crown was used for ful-crown restoration. Fracture strength and fracture type were recorded in different groups.
RESULTS AND CONCLUSION: The fracture resistance was higher in the cobalt-chromium cast post and core group and zirconium oxide post and core group than the glass fiber post and core group (P < 0.05), and the former two groups had no significant difference. Cobalt-chromium cast post and core was fractured at the root of tooth, and could not be repaired; the zirconium oxide post and core was fractured at the root neck and root of tooth, which was confirmed as reparative fracture; the glass fiber post and core was fractured at the tooth neck, which could be restored. These findings indicate that the cobalt-chromium cast post and core can bear greater occlusal force, but has a higher probability of root fracture; the CAD/CAM-fabricated glass fiber post and core exhibits a lower probability of root fracture

BACKGROUND:Currently, there are many studies on colapsed repair, but a systematic and horizontal comparison is not reported yet. OBJECTIVE: To compare the effect of conventional resin adhesive, resin adhesive+silane coupling agent and adjacent surface open part of the crown on colapsed repair through clinical trial and in vitro experiment. METHODS: (1) Clinical trial: 90 patients with porcelain colapse were randomized into three groups, and respectively treated with conventional resin adhesive, resin adhesive+silane coupling agent and adjacent surface open part of the crown. Success rate was measured and compared among three groups at 1 year after repair. (2) In vitro test: Twenty test specimens were equaly divided into two groups, and treated with conventional resin adhesive and resin adhesive+silane coupling agent, respectively. Then, shear strength was detected in the two groups. Twenty double-crown specimens were equaly divided into four groups. The first three groups were treated with sand blasting, silane coupling agent and their combination treatment, respectively; the rest group had no treatment (control group). After repair, the tensile strength of each specimen was detected.RESULTS AND CONCLUSION:The success rate of colapsed repair was 37% for conventional resin adhesive, 90% for resin adhesive+silane coupling agent and 100% for adjacent surface open part of the crown. The shear strength was (13.978±0.343) MPa for the conventional resin adhesive and (10.058±0.64) MPa for resin adhesive+silane coupling agent, and there was a significant difference between two methods (P < 0.01). The tensile strength was (0.68±0.04) kN in the control group, (1.00±0.02) kN in the sand blasting group, (1.31±0.08) kN in silane coupling agent group, and (1.09±0.04) kN in the combination group, and there was a significant differences between groups (P < 0.01). Experimental results show that the silane coupling agent+resin adhesive treatment and adjacent surface open part of the crown are superior to conventional resin adhesive.

Administration, Rectal ; Adolescent ; Adult ; Aged ; Chronic Disease ; Drugs, Chinese Herbal ; administration & dosage ; Humans ; Male ; Middle Aged ; Phytotherapy ; Prostatitis ; drug therapy ; Suppositories

This article briefly described the existing problems in talent training of TCM informatics, conducted discussion according to discipline personnel training practice teaching mode, proposed to introduce Arduino open-source hardware into TCM informatics teaching, try to carry out practice exploration for existing classroom and experiment teaching, and proposed relevant suggestions to promote learning interest of students and improve their application ability, with a purpose to further improve the practice teaching mode of TCM informatics, cultivate "TCM+informatics" compound and application talents to promote long-term development of this discipline.

BACKGROUND: As a cytokine highly expressed in internal organs, visfatin could be used as a biomarker of systemic inflammation response for chronic obstructive pulmonary diseases, but few studies have reported the use of visfatin in severe pneumonia. The present study was undertaken to determine the plasma levels of visfatin in patients with severe pneumonia. METHODS: A total of 70 patients, including 40 patients with severe pneumonia (group A) and 30 patients with non severe pneumonia (group B) who had been admitted to the ICU from June 2009 to June 2010, were enrolled in this prospective study. And another 30 healthy physical examinees served as healthy controls (group C). Patients were excluded if they suffered from severe diseases of the heart, brain and kidney, cancers, autoimmune diseases, or received special treatment in the latest month. The plasma levels of visfatin, IL-6, IL-8 and TNF-α were measured by ELISA, while the level of CRP was determined by immuneturbidimetry, and the routine blood test was performed. Blood gas analysis and Acute Physiology and Chronic Health Evaluation II (APACHE II) were performed in patients with pneumonia. Comparisons between the groups were conducted by Student's t test, ANOVA or nonparametric test. Correlation analysis was carried out by Pearson's correlation test or Spearman's rank-order correlation test. RESULTS: The plasma level of visfatin in group A was significantly higher than that in groups B and C (P<0.001), and the level of visfatin in group B was significantly higher than that in group C (P<0.001). The plasma level of visfatin was positively correlated with CRP, TNF-α, APACHE II and PMN％ in patients with severe pneumonia (rho=0.653, r=0.554, r=0.558, r=0.484, respectively, P<0.05 for all), while it was negatively correlated with PaO2 and PaO2/FiO2 (rho=?0.422, r=?0.543, respectively, P<0.05 for all). CONCLUSION: Visfatin may be involved in the systematic inflammation response in patients with severe pneumonia as a pro-inflammatory cytokine, and it is valuable in assessing the severity of pneumonia..

AIM: To observe the effects of berberine (Ber) on enterocyte apoptosis in septic mice and its pos-sible mechanism.METHODS: Male C57BL/6 mice (8 ~10 weeks old) were randomly divided into sham group, cecal ligation and puncture (CLP) group, CLP +Ber group and sham +Ber group.The mice in CLP group underwent CLP ope-ration, and the mice in sham groups suffered a similar operation except the ligation and puncture.After the sham or CLP operation, the mice were administered intragastrically with distilled water or berberine (50 mg/kg) within 2 h.After 20 h, the mice were killed with excess pentobarbital sodium and the ileum tissues were removed.The histological changes of the intestine were observed and the enterocyte apoptosis was examined by determining the protein level of cleaved caspase-3. Furthermore, mitochondrial Bax, cytoplasm cytochrome C (Cyt C) and the total proteins of Bcl-2, Fas, FasL and Fas-as-sociated protein with death domain (FADD) were examined by Western blot.The mRNA expression of tyrosine hydroxylase (TH) and dopamine beta-hydroxylase (DBH) was measured by real-time PCR.RESULTS: The extensive ileum injuries, including remarkably increased leukocytes and necrosis of intestinal villus were observed 20 h after CLP.In CLP group, the protein levels of cleaved caspase-3, cytoplasm Cyt C, as well as Fas, FasL were significantly increased, but the Bcl-2 level was decreased.Bax translocation into mitochondria was promoted.However, FADD was not changed significantly.The mRNA expression of TH and DBH was also increased sharply in CLP group.On the contrary, treatment with berberine made a considerable alleviating alteration in the ileum of the septic mice.CONCLUSION: Treatment with berberine pro-vides protective effects on intestinal injury in septic mice by reducing enterocyte apoptosis, and its possible mechanism may be involved in the inhibition of the endogenous and exogenous apoptosis pathways.