OBJECTIVE:To apply the biosensor technology to screening of the best method to extract and separate anti-endotoxin effective materials in Radix Paeoniae rubra.METHODS:The affinities of samples extracted by three different methods binding to lipopolysaccride(LPS)were determined with biosensor technology.Extracted materials were incubated with0.25EU/ml endotoxin for determination of change of binding activity.RESULTS:Material extracted by water showed high binding capa?bility with LPS,after incubation with endotoxin,still had highly effective anti-endotoxin component;1∶40diluted water extract could neutralize78.1%endotoxin.Determination results by biosensor technology and traditional limulus reagent showed no dif?ference.CONCLUSION:Water extraction could obtain more anti-endotoxin effective materials.Compared with traditional methods,biosensor technology is a fast,highly effective,direct and accurate method.

Hydroxypropyl methylcellulose (HPMC) propels self-emulsifying drug delivery systems (SEDDS) to achieve the supersaturated state in gastrointestinal tract, which possesses important significance to enhance oral absorption for poorly water-soluble drugs. This study investigated capacities and mechanisms of HPMC with different viscosities (K4M, K15M and K100M) to inhibit drug precipitation of SEDDS in the simulated gastrointestinal tract environment in vitro. The results showed that HPMC inhibited drug precipitation during the dispersion of SEDDS under gastric conditions by inhibiting the formation of crystal nucleus and the growth of crystals. HPMC had evident effects on the rate of SEDDS lipolysis and benefited the distribution of drug molecules across into the aqueous phase and the decrease of drug sediment. The mechanisms were related to the formed network of HPMC and its viscosities and molecular weight. These results offered a reference for selecting appropriate type of HPMC as the precipitation inhibitor of supersaturatable SEDDS.

Objective To investigate the transcription factor 21 (transcription factor 21 ,TCF21) gene on human lung cancer A549 cells sensitivity to chemotherapy .Methods Using lentivirus technology in A549 lung cancer cells highly expressed genes TCF21 ,fluorescence quantitative PCR ,Western Blot analysis were used to analyse the expression of the target gene ,MTT assay was used to detect the effect of TCF21 lung adenocarcinoma A549 cells on cisplatin (cis‐Dichlorodiamineplatinum ,DDP) chemosen‐sitivity ,and colony assay was used to detect the effect of overexpression of TCF21 lung adenocarcinoma A549 cells on radiosensitiv‐ity .Results After 72 h ,with the increasing concentration of DDP (0 ,0 .625 ,1 .250 ,2 .500 ,5 .000 ,10 .000 mg/L) ,corresponding in‐hibition rates in each group increased ,and the inhibition rate of the high expression group was significantly higher in empty vector group and untransfected group (P<0 .05) ,no significant difference between the two then;overexpression TCF21 group with drug concentration and time and increase the rate of high expression inhibition corresponding increase (P<0 .05) ;after receiving X radi‐ation ,non‐transfected group ,untransfected plus radiotherapy group ,vector group ,vector plus radiotherapy group ,high expression and high expression + radiotherapy colony formation rates were:95 .17% ± 2 .85% ,88 .20% ± 2 .03% ,93 .80% ± 4 .17% , 85 .60% ± 2 .42% ,71 .67% ± 3 .21% ,56 .00% ± 2 .65% .Conclusion TCF21 gene expression can significantly enhance the sensitiv‐ity to radiotherapy and chemotherapy DDP A549 lung cancer cells .

In recent years, 40Hz auditory steady state response (ASSR) has been paid more attention when the depth of anaesthesia monitored because its same source with the middle latency auditory evoked potential (MLAEP), strong anti-jamming ability and close relationship with consciousness level. This article brings forward some effective and steady methods for extracting ASSR according to its characteristics. The results of these methods are analyzed and compared based on the actual records from clinical practice. And thus an extraction method fit for monitoring the depth of anaesthesia is determined.

It is very significative to perform real-time and effective monitor of the depth of anesthesia during the clinical operation. With the introduction of muscle-relaxing agents in general anesthesia, the two major indices to judge the depth of anesthesia have been obscured, which makes it more difficult to judge the depth of anesthesia. In recent years it has been found that 40Hz Auditory Steady State Response (ASSR) has a very good characteristic to monitor the depth of anesthesia. This article makes use of parametric-model method to extract 40Hz ASSR, which has a higher speed than Moving Time Average (MTA) when tracing the transition process of the patient from being conscious to being unconscious.

Objective To analyze palm ratio of body surface area (B SA ) based on the body different surface area form ula. Methods Forty-four Chinese adult m ales w ere selected in this study. The contour of left single palm w as recorded and the palm area w as calculated by cut-paper w eighing m ethod. The body surface w as calculated by the height and w eight based on 12 form ulas of body surface area. Then, the palm ratio of BSA w as calculated. Results The average palm ratio of BSA w as (0.789±0.075)% . The data show ed statistically significant difference com pared with the traditional ratio (1% ) (P<0.05). Conclusion The ratio of B SA in Chinese adult m ale population show ed m ore accuracy com pared with the traditional ratio in this present study. The forensic evaluation can be affected by using the different ratio of B SA .

Objective To investigate the effect of TCF21 on human non-small cell lung cancer A549 cells , and to reveal the role of TCF21 in the development of NSCLC. Methods Overexpression of TCF21 in NSCLC A549 cells was mediated by lentivirus vector. TCF21 protein expression was identified by Western blotting assay. The experimental group, negative control group and the blank control group were set up. A549 cells were subcuta-neously seeded in BALB/c nude mice on the left armpit. Results TCF21 was successful overexpressed in the ex-perimental group. Compared with the negative control group and the blank control group , the tumor growth was slow, and the final tumor volume was significantly reduced in the experimental group. Conclusion Overexpression of TCF21 can inhibit the tumor growth of NSCLC in nude mice , indicating that TCF21 may play an important role in NSCLC development.

Objective To investigate the expression of EphA8 in colon cancer and its clinical significance.Methods Real-time quantitative polymerase chain reaction (RT-PCR) and Western blot were respectively used to assess the expressions of EphA8 mRNA and protein in normal colon mucosa cell line and colon cancer cell lines.Immunohistochemistry for EphA8 and vascular endothelial growth factor (VEGF) were performed in 98 cases of colon cancer and 12 matched normal mucosa tissues.CD31 immunohistochemical staining was used for microvascular density (MVD) counting.The relationships between the expression of EphA8 and the expression of VEGF and MVD,the clinical pathological significance,and the prognosis of patients were analyzed by statistical methods.Results EphA8 mRNA expressions were significantly higher in colon cancer cell lines than those in normal mucosa cell line (t =11.98,13.54,P ＜0.001).EphA8 protein expressions were significantly higher in colon cancer cell lines than those in normal mucosa cell line (t =4.63,P =0.006;t =4.92,P =0.004).The high expression of EphA8 was closely related to tumor size (x2 =22.97,P＜0.001),TNM stage (P＜0.001),differentiated degree (P =0.007)and lymph node metastasis (P＜0.001),distant metastasis (x2 =6.97,P =0.008) and poor survival rote (x2 =17.3,P ＜0.001).But it was not associated with the gender and age (x2 =1.36,P =0.30; x2 =0.83,P=0.44).Besides,EphA8 had positive correlation with the VEGF and MVD (r =0.434,P ＜0.001; r =0.584,P ＜0.001).Conclusion EphA8 may play important roles in the development and metastasis of colon cancer,which has potential clinical values in the therapeutic intervention and prognosis of colon cancer.

Objective To compare and analyze the different clinical features between male and female patients with rheumatoid arthritis (RA), by detecting the gender difference to make clinical decisions. Method The clinical features of 95 male RA patients(MRA) were analyzed and compared with those of 248 female RA patients (FRA) by retrospective analysis method. Results The mean onset age of MRA [18-79 (51.40±15.55) years old] was older than that of FRA[18-75(43.54 ±15.46) years old](P=0.0002). The morning stiffness time of MRA [(1.58 ±1.34) h] was shorter than that of FRA [(2.10 ±1.51) h](P = 0.0357). There was statistical difference in the first involved joint between MRA and FRA (P=0.0170), the prevalence of knee,metatarsonphalangeal joint,shoulder and ankle of MRA was higher than those of FRA, ' but conversely the wrist prevalence of FRA was higher than that of MRA.The prevalence of secondary Sj(o)gren syndrome of MRA(13.68%, 13/95) was rather lower than that of FRA(35.08%, 87/248)(P = 0.0007). MRA had lower positive rate of ANA (2.11% ,2/95) and lower value of IgM [(1.42 ±0.75) g/L] than FRA [(10.48% ,26/248), (1.69 ±0.86) g/L] (P < 0.05). There was no statistical difference between MRA and FRA in articular radiographic changes, most extraarticular manifestations and lab results (P >0.05). Conclusions MRA patients have older onset age and milder articular symptom than FRA patients. The disease severity of MRA is similar to that of FRA. Weighted joints are more involved in MRA. FRA is more inclined to have secondary Sj(o)gren syndrome.