Objective To evaluate the diagnostic efficacy of the reformatted unenhanecded spiral CT of ureter for ureterolithiasis. Methods 39 patients were examined using nonenhenced spiral CT from the top of the kidneys to symphysis pubis in a single breath hold. The postprocessing method used in our studies was curved planar reformatting(CPR). Results Reformatted CT displayed clearly the entire course of ureter in all 39 cases . The diagnosis of ureterolithiasis was made on CPR views by definitive demonstration a calculus actually within the ureter, including the stone size, shape, location and the obstruction degree of ureter in 33 patients. The CPR views also demonstrated the ureter and the structures around clearly to aid greatly in differentiating the other 6 cases miscellaneous urinary tract conditions. Conclusion Our findings suggest that the reformatted nonenhanecded spiral CT of ureter has potential as an imaging tool for the ureterolithiasis and other urinary tract abnormalities.

Objective:To evaluate the feature of juvenile onset recurrent respiratory papillomatosis (JORRP).Methods:Thirty-eight cases with JORRP were analyzed retrospectively from June,1986 to June,2000.Results:The average age was 3.5 years before diagnosis.Average 3.45 operations had been performed per patient .Of 38 cases ,eight had comlications of laryngeal dysfunction after operations.Peristomal and tracheal papilloma were spread in six of the nineteen cases with tracheotomies.Conclusion:JORRP is the most common laryngeal tumor in children. Most patients have frequent recurrences and multiple operations. Care should be taken to avoid damaging the normal laryngeal structure during surgical excision.

Aim To investigate the effects of ?,?-methylene ATP ( ?,?-MeATP) on the P2X1 purino-ceptor-mediated vasoconstriction by different administration ways in the rat mesenteric artery. Methods Isometric vasoconstrictive responses to ?,?-MeATP,administered in non-accumulative manner or single concentration manner,were recorded in the rat isolated mesenteric arterial rings. Results ?,?-MeATP ( 10 -7 ～ 10 -4 mol ? L -1 ) administered in both of the two manners produced concentration-dependent vasocon-strictive responses in the rat isolated mesenteric artery. The vasoconstrictive responses to ?,?-MeATP in single-concentration administration group were greater than those in non-accumulative administration group when the vasoconstriction was standardized either by tissue wet weight or by maximal response to 120 mmol ?L-1 KCl ( P

Cells, Cultured ; Endothelial Cells ; drug effects ; metabolism ; Endothelium, Vascular ; cytology ; metabolism ; Gene Expression Profiling ; Humans ; Ouabain ; pharmacology ; Umbilical Veins ; cytology

Objective To evaluate the application of CT perfusion in the assessment of muscle perfusion in ischemic rabbit hindlimb treated by implantation of bone marrow mesenchymal stem cells.Methods Twenty rabbits were divided into two groups randomly (each n =10).Bone marrow mesenchymal stem cells were injected into the left ischemic hind limbs of animal models of intravenous injection group,with same volume of normal saline injected into the control group.Twenty-eight days later,the collateral circulation and blood vessel neogenesis were examined by Toshiba Aquilion One 320-MSCT and pathological approaches.t test and Pearson test were used to compare the parameters.Results The results show that rAF,rBV,rClearance,rMVD were 1.15 ±0.67,1.19 ±0.32,0.62 ±0.20,and 1.34 ±0.28 in intravenous injection group and 0.57 ±0.17,0.74 ±0.19,2.06 ±0.15,0.62 ±0.19 in the control group respectively.There was significant difference of rAF,rBV,rClearance and rMVD between the intravenous injection group and the control group(t =5.75,5.01,-2.81,6.43 respectively,P ＜ 0.01).There was significant correlation between rBV,rAF,rC and rMVD (r =0.857,0.811,0.615,P ＜ 0.01).Conclusion CT perfusion imaging is a relatively accurate technique to assess changes of muscle perfusion in ischemic rabbit hindlimb treated by implantation of bone marrow mesenchymal stem cells.

Objective To explore the effects of high glucose on release of endothelial microparticles (EMPs) and cell apoptosis in human umbilical vein endothelial cells (HUVECs). Methods HUVECs were incubated for 0h, 12h, 24h, 48h and 72h, respectively, with different concentrations of glucose (5.5mmol/L, 16.7mmol/L and 33.3mmol/L, respectively). The cells cultured in the media containing glucose 5.5mmol/L and mannitol 27.8mmol/L served as control groups. The survival rate of cells was examined by MTT assay. The level of EMPs release and percentage of cell apoptosis were detected by flow cytometry. Results ① According to MTT detection, the survival rate of HUVECs was 93% when incubated for 12h with 33.3mmol/L, 78% for 24h, 51% for 48h, and 40% for 72h. Compared with that for 0h, the survival rate for 12h did not decline significantly (P>0.05). the survival rate reduced significantly for 24, 48 and 72h, respectively, in the time-dependent manner (P<0.05). ② If the survival rate was 100% when incubated in 5.5mmol/L glucose for 48h, then it was 98% in mannitol 27.8mmol/L with no significant difference (P>0.05). It was 79% in 16.7mmol/L glucose and 51% in 33.3mmol/L glucose, obviously lower than that in 5.5mmol/L glucose, and the two groups differed significantly (P<0.05). ③ the level of EMPs release increased significantly in 33.3mmol/L glucose for 12, 24, 48 and 72h respectively, in the time-dependent manner. Compared with the normal glucose group, the level of EMPs release did not significantly differ in mannitol 27.8mmol/L for 12, 24, 48 and 72h , respectively (P>0.05). ④ There was a significantly positive correlation between EMPs release and cell apoptosis (r=0.659, P<0.05). Conclusion High glucose induces release of HUVECs endothelial miccroparticles and promotes cell apoptosis.

Cells, Cultured ; Drug Antagonism ; Drug Resistance, Multiple ; drug effects ; HSP70 Heat-Shock Proteins ; pharmacology ; Humans ; K562 Cells ; drug effects ; pathology ; Quercetin ; pharmacology

Objective To discuss the influences of irbesartan on high glucose-induced lipid oxidation effect and human umbilical vein endothelial cell (HUVEC) injury. Methods HUVECs of the logarithmic phase were divided into four groups:normal-concentration glucose control group (glucose final concentration of 5.5 mmol/L),high-glucose group (glucose final concentration of 33.3 mmol/L),irbesartan intervention group,and anti-oxidant N-acetyl-cysteinyl acid (NAC) intervention group. The intervention groups were first treated with irbesartan (10-5mmol/L) and NAC (10 mmol/L) for 1 h,respectively,then co-incubated with high glucose (glucose final concentration of 33.3 mmol/L) for 24 h,48 h and 72 h. Malondialdehyde (MDA) content and superoxide dismutase (SOD) activity of culture supernatant were determined by TBARS method and spectrophotometric assay,respectively. Results MDA content increased significantly (P0.05). Compared with that in high glucose group,MDA content significantly decreased (P

Objective Through death analysis of critically ill children with interhospital transportation,to explore the cause of death and its influencing factors.Methods We collected the data of death cases within 24 hours who were one-way transported from primary hospital to the emergency department of Guangzhou Women and Children's Medical Center between July 2012 and May 2014.We analyzed the diseases type,physiopathologic status,and the treating measures in the process of transportation.Results There were total 1 122 cases,34 children died,the mortality was 3.0％,7 cases died before admission,27 cases died within 24 hours after admission.The first three types of diseases were respiratory system diseases(8/34,23.5％),nervous system diseases (7/34,20.6％) and accidental injury (6/34,17.6％).The physiopathologic status were analyzed according to physiological status and laboratory data at admission.Most of the children had a variety of physiopathologic status.Electrolyte disturbances were found in 22 cases(81.5 ％),metabolic acidosis and respiratory failure in 19 cases (70.4％).Most cases had intravenous channel (33/34,97 ％),intravenous infusion (32/34,94.1％)and electrocardiogram monitoring (30/34,88.2％),none of the cases received vasoactive agents in transportation.Conclusion Insufficient disease evaluation before the transportation of critically ill children,inadequate monitoring and treating measures on the way are important factors causing the death of children.

Objective To review the diagnosis and treatment of primary gallbladder carcinoma in patients older than 70 years.Method The clinical data of 48 patients older than 70 years with primary gallbladder carcinoma treated in our hospital from 2003 to 2010 were retrospectively analyzed.Results The preoperative diagnostic rate was 60.4％.Of 48 patients with primary gallbladder carcinoma,9 received cholecystectomy,18 radical resection,7 extended radical resection,8 palliative operation,while the remaining 6 received biopsy because of metastasis. The 3-year survival rate was 20.8％ and the 5-year survival rate was 8.3％.Conclusions Regular B-ultrasonography,CT-scan and MRI are suggested for elderly patients with chronic diseases of the gallbladder.Radical resection is recommended if clinically possible. Chinese traditional medicine given postoperatively improved the prognosis.