China ; Humans ; Medicine ; methods ; trends ; Medicine, Chinese Traditional ; trends

To address the role of Pr1 gene in the process of Hirsutella sinensis infecting Hepialus gonggaensis, differential expression of subtilisin-like protease gene was detected. In the present study, Pr1 gene analogues from H. sinensis were obtained by PCR strategy using specific primers designed from conserved regions of Pr1 gene reported in the GenBank. Then we detected the changes in the expression of Pr1 gene before and after infecting H. gonggaensis using real-time quantitative PCR. We obtained the partial sequence of Pr1 gene with the length of 535 bp (GenBank accession: KC009680). Real-time PCR results showed that the expression level of Pr1 gene was significantly different among 8 samples (P < 0.01). Pr1 gene showed the obvious higher expression level (2-3 folds) after infecting the H. gonggaensis, suggesting that the Pr1 gene may play an important role in the process of H. sinensis infecting H. gonggaensis. The present study paves a way for further identification on infectivity assessment of H. sinensis.

[Objective]To explore the optimal time for the surgical operation on patients with ossification of the posterior longitudinal ligament of the cervical spine. [Methods]The clinical symptoms,the persistent time of the symptoms and the interval from exacerbation to operation in 168 patients with OPLL were analyzed.[Results]The average time was 27.6 months from the onset of myelopathy to the operation on patients with OPLL,and 17.4 months from the decrease of muscle strength in extremities to the operation,and 5.3 months from the symptomatic exacerbation of the extremities especially the decrease of muscle strength in lower extremities to the operation.[Conclusion]The patient should be treated earlier by surgical operation after his/her illness has been diagnozed as OPLL.It is the optimal time for surgical operation when the patient has imaging change of hypertrophy or ossification of the posterior longitudinal ligament and severe compression of spinal cord(more than 60% spinal canal stenosis),or the decrease of muscle strength in extremities especially in lower extremities.

Objective A rapid method was constructed for detection of 3 kinds of phosphodiesterase 5 (PDE5) inhibitors (sidenafil,vardalafil,tadalafil) in invigorative health food by paper spray ionization mass spectrometry (PSI-MS method).Methods The characteristic ions of the PDE5 inhibitors could be used for preliminary identification and semiquantitative analysis with internal standard method using PSI-MS method.Results The 24 kinds of commercially available health-care products includes capsule,tablet,pill,powder,wine,syrup and liquid were tested.Results from PSI-MS were consistent with the HPLC-UV date.The calibration curves of PSI-MS has a good linearity in a given range.The linear coefficients of analytes were higher than 0.99.The LODs of 3 kinds of PDE5 inhibitors were lower than 1.0 mg/L.The RSDs of this method ranged from 20％ to 24％.Conclusion The PSI-MS method was rapid,accurate and targeted,which is compliant for quickly screening the PDE5 inhibitors in large complex matrix samples.

Objective To assess the status of mental health of nursing students of secondary technical school and provide targeted guidance for mental health education. Methods 642 nursing students of secondary technical school were assessed using Mental Health Diagnostic Test Scale (MHT Scale). Results There were 0.78% of nursing students with serious psychological problems (scores≥65), 4.52% of nursing students with less serious psychological problems (scores < 65 and≥ 56). Students in different grades had significant difference in learning anxiety, allergic tendency, physical symptoms anxiety. Conclusions Nursing students of secondary technical school were in good mental health in general, but there were many students had learning anxiety, allergic tendencies and physical symptoms anxiety, which were different among various grades. Secondary nursing schools should further strengthen the mental health education, and seek close collaboration with parents,provide appropriate intervention measures to the nursing students with psychological barriers, in order to enhance the overall mental health of nursing students.

AIM: To observe the effect of compound clindamycin liniment on acne vulgaris. 　METHODS: A multi-centre, open-label trial of compound clindamycin liniment in treating acne vulgaris. One hundred and fifty-three patients used compound clindamycin liniment on face three times per day for 4 wk.　RESULTS: The total excellent response rate was 83.0%. The total effect rate on pustule and papule was 96.1% and 80.7% respectively. The adverse reaction rate was 1.9%.　CONCLUSION: Compound clindamycin liniment is an effective and safe drug in the treatment of acne vulgaris.

Objective To summary the experience in diagnosis and surgical treatment of differentiated thyroid carcinoma (DTC). Methods The clinical data and follow-up results of 167 cases of DTC were retrospectively analysed. Results (1) The accurate rate of preoperative cytology diagnosis was 76.9%(97/126). (2)121 patients were diagnosed as thyroid carcinoma before or during operation. Histologic examination after primary operation revealed that the carcinoma spread to the isthmus and the opposite lobe in 5 cases. 57 cases were followed up with only 2 cases recurred 2 and 3 years after the operation, and 3 died 6 to 10 months after operation. (3)87.5%(42/48)cases of the reoperation were due to misdiagnosis as benign lesions in primary operation. Histologic examination revealed that residual tumor in the remnant thyroid tissue was found in 45.2%(19/42)cases, and residual tumor in the lymph nodes of the suffered side in 19.0%(8/42)cases. 30 cases were followed up without any recurrence. Conclusions (1) Cytological diagnosis must be paid great attention to preoperatively; (2) Excision of the suffered lobe, isthmus and greater partial of the opposite lobe, along with clearing the enlarged lymphnodes of the isolateral side are generally performed for the DTC; (3) Reoperation due to misdiagnosis as benign lesions in primary operation must remove the remnant tissue of thyroid and the isolateral lymphnodes. The cases should also be followed up even if enough excising is performed in primary operation.

Objective: To investigate the expression and clinical significance of Ezrin, a tumor metastasis related gene, in tissues of nasopharyngeal carcinoma (NPC). Methods: The expression of Ezrin was detected with two-step immunohistochemistry technique in tissues of 38 NPC patients and 20 nasopharyngitis patients. All of the Ezrin expression and clinical data (gender, age, clinical stage, recurrence, and ]ymphonode metastasis) were compared and analyzed between two groups. Resuits: The Ezrin expressions were significantly different between NPC tissue (and peri-NPC tissue) and naeopharyngitis tissue (P＜0.05). No significant difference was found in the Ezrin expressions between the NPC tissue and the peri-NPC tissue (P＞0.05). It was found by Spearman analysis method that the Ezrin expression increased with patients' age (P＜0.05), but no significam relationship with gender, clinical stage, recurrence, and lymphonode metastasis (P＞0.05). The Ezrin expression influenced the survival time of NPC patients significantly (P＜0.01). Conclusion: The Ezrin expression can be an useful prognostic factor for NPC patients.

Objective To investigate scanning and reconstruction techniques of multi-slice spiral CT (MSCT) in patients with complex congenital heart diseases (CCHD).Methods One hundred eighty-four patients suffering from CCHD underwent 16-detector MSCT scanning without ECG-gating.Multi-planar reconstruction (MPR),maximum intensity projection (MIP),curved-planar reconstruction (CPR) and volume rendering (VR) were used to reconstruct images.CT findings were compared with those of surgical operation or angiocardiography.Results A total of 616 cardiac deformities were found with MSCT and proved by angiocardiograms or surgical operation.The diagnostic accuracy of extracardiac malformation with MSCT was 100%,of atrial septal defect was 54.65%,and of ventricular septal defect was 78.62%.MSCT failed to display heart valve disease well.Conclusion MSCT can accurately detect extracardiac malformations of CCHD.