1.Analysis on IgM antibody detection results of pathogens causing respiratory tract infections during first half year in Shihezi area
Lu WANG ; Min LIU ; Jiang CHENG
International Journal of Laboratory Medicine 2015;(7):895-896
Objective To understand the prevalence situation of common respiratory tract pathogens in Shihezi area to provide reliable basis for clinical diagnosis and treatment Methods The serum samples from the inpatients with acute respiratory tract in‐fection in the First Affiliated Hospital of Medical College of Shihezi University from January to June 2014 were collected and detec‐ted 9 kinds of common pathogens by using the indirect immuno‐fluorescence assay .Results Among 810 serum samples ,the IgM antibody positive detection rate was 32 .35% ,the detection rates of various pathogens from high to low were Mycoplasma pneumon‐iae(MP ,21 .48% ) ,Q fever rickettsia (COX ,8 .8% ) ,legionella pneumophila(LP1 ,5 .18% ) ,Chlamydia pneumoniae(CP ,4 .2% ) ,in‐fluenzaBvirus(INFB,2.22% ),parainfluenzavirus(PIVs,1.24% )andrespiratorysyncytialvirus(RSV,0.50% ).MPinfectionwas dominated by young children ,the detection rate in females was higher than that in males (P<0 .05);majority of COX infection were young adults ,the detection rate in males was higher than that in females (P<0 .05) .Conclusion MP is the main respiratory tract infection pathogen in children and COX is the main respiratory tract infection pathogen among young adults in Shihezi area .
2.Retrospective Study on the Protocol Deviation in Drug Clinical Trial Implementation of Our Hospital
Jinhua LIU ; Min LIU ; Yucun LIU ; Jun DUAN ; Yi LU
China Pharmacy 2017;28(25):3474-3478
OBJECTIVE:To study the influencing factors of protocol deviation in drug clinical trial implementation,and pro-vide reference for improving the quality of drug clinical trial. METHODS:Quality verification was conducted for the drug clinical trial projects in the First Affiliated Hospital of Chongqing Medical University during 2010-2016,and protocol deviations in each year were retrospectively studied,classified and analyzed. Category,frequency,international and domestic pilot projects and the differences of protocol deviation after full-time research nurse participating in trail management were explored,and the measures were put forward. RESULTS:27 drug clinical trials were implemented in our hospital during 2010-2016,including 949 cases,176 cases with protocol deviation,accounting for 18.55%. Deviation protocol in drug clinical trial was decreased year by year for 7 years. The categories were mainly incompleteness of observation/records (30.11%),checking omission/broaden the window(28.41%),adverse drug events and improper combined medication (14.20%) and omission in drug management (11.93%). The proportion of protocol deviation with full-time research nurse participated was lower than the projects without full-time research nurse(11.11% vs. 28.67%,P<0.01),and proportion of deviation protocol in international multi-center project was lower than the domestic projects(6.60% vs. 28.84%,P<0.01). CONCLUSIONS:It is suggested to pointedly strengthen the weak links of drug clinical trial. For example,clinical trial institutions should establish the clinical trial data retention system,electronic data should be timely backed up in a different places,etc. Besides,clinical trial institutions should equip professional full-time research nurses as much as possible,learn the rigorous scientific experimental design,standard operational procedures and the authenticity of data pro-cessing from the international multi-center clinical trial projects to effectively reduce the incidence of deviation protocol and im-prove the quality of drug clinical trials.
3.EXPERIMENTAL STUDIES ON THE EFFECTS OF TSCP, TSCC ON BLOOD PLATELET FUNCTION AND THEIR MECHANISM
Qingyun LIU ; Daiyin PENG ; Min LU ; Min DAI ;
Chinese Pharmacological Bulletin 1987;0(03):-
The effects of TSCP and TSCC on aggregation and adhesion of blood platelets were measured by turbidimetric method and salzman method respectively. TSCP and TSCC strongly increase mouse and rat platelet aggregation and platelet adhesion. These effects are important for blood coagulation. cAMP and TXB2 levels in plasma and platelet were determind by RIA TSCP significantly increased the TXB2 level in plasma and platelet. This effect may be the main mechanism of increasing platelet aggregation by TSCP.
4.Prostate cancer: diagnostic value of quantitative analysis by dynamic contrast-enhanced MR at 3.0 T
Wei HE ; Yi LIU ; Jianyu LIU ; Min LU
Chinese Journal of Radiology 2014;48(3):215-218
Objective To investigate the diagnostic value of the quantitative analysis parameters of dynamic contrast-enhanced MRI (DCE-MRI) for prostate cancer.Methods MR examination were performed in 26 patients and correlations were made with surgical pathology following radical prostatectomy.According to the localization of pathologic specimens,ROI were drawn on areas of cancerous regions,noncancerous regions in peripheral zone and central gland to measure the values of Ktrans,kep and Ve.The values of the three parameters in different tissue were compared with Kruskal-Wallis H and Mann-Whitney U.Results Twenty six patients had prostate cancer confirmed by pathology.Data from 468 assessable regions of prostate were acquired,including 115 cancerous regions and 142 noncancerous regions in central gland,68 cancerous regions and 143 noncancerous regions in peripheral zone.Prostate cancer showed a multifocal distribution.The K,kep and Ve values were (1.04 ± 0.26)/min,(1.43 ± 0.46)/min,(0.76 ±0.12) respectively for cancerous regions in central gland,and (0.82 ±0.19)/min,(1.12 ±0.26)/min,(0.75 ± 0.14) respectively for noncancerous regions in central gland,(0.95 ±0.31)/min,(1.31 ±0.51)/min,(0.76 ± 0.13) respectively for cancerous regions in peripheral zone and (0.32 ± 0.07)/min,(0.52 ± 0.13)/min,(0.64 ± 0.14) respectively for noncancerous regions in peripheral zone.The differences among the three parameters were statistically significant (x2 =316.008,297.985,63.681,P <0.01).The Ktrans,kep values of cancerous regions were significantly higher than the corresponding values of noncancerous regions,respectively.Conclusion Quantitative analysis parameters of DCE-MRI contribute to the diagnosis of prostate cancer,including cancer located in central gland.
5.Lafora disease: a report of five cases diagnosed by skin biopsy
Yuwei DA ; Yan LU ; Yueshan PIAO ; Aihua LIU ; Dehong LU ; Yuping WANG ; Min WANG ; Min XU ; Lu LIU ; Jianping JIA
Chinese Journal of Neurology 2008;41(7):468-470
Objective To investigate the clinical manifestions, pathological features and diagnosing methods of Lafora disease. Methods The chnical and pathological features of 5 patients with Lafora disease who were diagnosed by axillary biopsies were systemically studied. The specimen were stained by HE, PAS and AB-PAS methods. Results Four of 5 cases had an onset during adolescence and 1 during adulthood. All cases presented with progressive generalized tonic-clonic seizure, myoclonus and dementia. Emotional disturbance, dysarthria and ataxia appeared in the early course of the disease. Lafora bodys were identified in myoepithelial cells and duct cells of both eccrine sweat glands and apecrine sweat glands in the biopsies of axillary skin. Conclusions Lafora disease could be confirmed by round and oval periodic acid-Schiff- positive inclusions in skin biopsy specimen combined with the proper clinical settings. Both axillary and other skins can be chosen as the sites of biopsy.
6.Sural Nerve Morphometrics of Motor Neuron Disease
Min XU ; Cunjiang LI ; Juexian SONG ; Yan LU ; Min WANG ; Lu LIU
Chinese Journal of Rehabilitation Theory and Practice 2009;15(1):15-16
Objective To investigate the pathological features of sural nerve biopsy in patients with motor neuron disease (MND). Methods 22 patients with amyotrophic lateral sclerosis (ALS) underwent sural nerve biopsy and routine electrophysiological examination. The transected images were captured and morphometrically analyzed.Results The myelinated fiber density decreased in ALS patients' sural nerves, and larger fibers were involved mostly. The thinly myelinated fibers increased. Conclusion The sural nerves of ALS patients shows mild but definite pathological changes.
8.Bacteria distribution and drug sensitivity analysis of nosocomial infection cases in children′s hospital
Zhuofu LIANG ; Lu KUANG ; Yongqiang XIE ; Min LIU ; Chunmiao LIAO
International Journal of Laboratory Medicine 2014;(11):1445-1446
Objective To understand and analysis of the pathogens distribution and drug resistance of nosocomial infection in children′s hospital,so as to provide reliable scientific basis for the prevention and control of hospital infection.Methods 396 cases of upper respiratory tract specimens were collected from pediatric patients with nosocomial infection.These specimens were detected by sputum specimens conventional methods of microorganism cultivation,and K-B method was used to determine the bacteria sensi-tivities to clinical common drug.Results There were 225 cases of specimens were pathogen positive among all the 396 specimens, and 234 strains of bacteria were isolated in all.The positive isolated rate was 56.8%(225/396).Among the 234 isolated strains, Gram negative bacteria accounted for 72.6%(170/234),and Klebsiella occupied the first place[49.4%(84/170)].Gram positive bacteria accounted for 23.5%(55/234),and Staphylococcus had the highest isolated rate in Gram positive bacteria[58.2% (32/55)].In all the 9 kinds of clinical common antimicrobial agents,imipenem had high drug sensitivity to the 234 isolated strains,and the aminoglycosides came next.Conclusion It is necessary for the pediatric patients with nosocomial infection to collect upper re-spiratory tract specimens for bacteriologic studies and drug sensitivity tests.
9.Effect of total saponins from rhizoma dioscoreae nipponicae on the expression of SDF1 and HCIKB kinase in rIK-1beta induced fibroblast-like synoviocytes.
Qi ZHOU ; Ning ZHANG ; Fang LU ; Shu-min LIU
Chinese Journal of Integrated Traditional and Western Medicine 2015;35(2):234-238
OBJECTIVETo study the effect of total saponins from Rhizoma Dioscoreae nipponicae (RDN) on the expression of stroma cell derived factor 1 (SDF1) and IKB kinase (IKK) in rIL-1beta induced fibroblast-like synoviocytes (FLS).
METHODSFLS were primarily cultured and the 3rd generation log phase growth FLS were divided into the normal control group, the model group, and the medication group. 10 microg/L rIL-1beta was used to induce the proliferation of FLS in the model group.10 microg/L rIL-1beta and 100 microg/L RDN were administered to co-incubate FLS in the medication group. No treatment was given to FLS in the normal control group. Expression levels of SDF1 and IkapaB kinase proteins (p-IKK) were detected using Western blot.
RESULTSExpression levels of SDF1 and p-IKK increased significantly higher in the model group than in the normal control group (P<0.01). Compared with the model group, expression levels of SDF-1 and p-IKK significantly decreased in the medication group (P <0.01).
CONCLUSIONSTotal saponins from RDN could inhibit the activation of both SDF1 and p-IKK. It might further regulate the expression of IKB kinase by regulating the expression of SDF1.
Cells, Cultured ; Chemokine CXCL12 ; metabolism ; Dioscorea ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Epithelial Cells ; Fibroblasts ; Humans ; Interleukin-1beta ; metabolism ; Saponins ; metabolism ; Synovial Membrane ; metabolism