Objective:To completely solve the traditional ECG examination mode, establish ECG diagnosis center in hospital, realize the automation of the whole process of ECG.Methods: Demonstrate the structure and function of Network ECG information management system software and hardware, describes the key technology used in the process of implementation.Results: Lead to centralized storage, management and diagnosis of the ECG data, achieve data sharing through integration with HIS, PACS system.Conclusion: Optimized the ECG examination process, improve doctor's efficiency and ECG report quality, make it convenient for the doctor to consult and analyze ECG data, provide medical care and powerful technical support for teaching and research.

Hospital digitization is a systematic process and has to be accomplished by stages. This paper discusses the key points of all stages of hospital digitization to enhance the control level of hospital.

Objective:To solve the management problems of using mobile cart, PDA, flat-panel computer and other mobile devices in hospital, promote the evolution of mobile device management to intelligent management. Methods: Select the mobile device management system for hospital use based on their own characteristics.Results: Manage all the mainstream operating system and terminal equipment; manage the hardware and software of mobile terminal and assets in hospital.Conclusion: Improve the management, reduce the maintenance workload, save the cost of operation and maintenance.

The improvement of quality control and management of Chinese medical equipment are discussed by analyzing the experience of other countries, aiming at the enhancement of medical equipment security, and social and economic benefit of the hospital.

Objective To investigate the mechanism of Acinetobacter baumannii resistant to carbapenems.Methods E test and microdilution were used to determine the minimum inhibitory concentrations(MIC) of 11 antibiotics against 30 isolates of imipenem-resistant Acinetobacter baumannii strains.The production of ?-lactamase was detected by improved three-dimensional test.Nine coding genes of?-lactamase were detected by PCR.Results The resistance rates of 11 antibiotics were over 90% except for polymyxin B(23.3%) and ciprofloxacin(87.7%).Among 30 isolates of Acinetobacter baumannii the positive rate of single ESBLs,single AmpC,ESBLs+AmpC,non-ESBLs non AmpC?-lactamase was 33.3%(10/30),3.3%(1/30),23.3%(7/30) and 26.7%(8/30) respectively by three-dimensional test.The coding genes:VIM-1,VIM-2,OXA-24,CTX-M-2,IMP-1 and VEB-1 were undetectable.Two or three of coding genes:OXA-23,PER-1 and AmpC were detectable in 27 isolates,and their homologies compared with corresponding sequences of GenBank were more than 98%.A nucleotide mutation(A→C) at 617 site of PER-1 gene was found in No.26 strain,so this sequence was registered in GenBank(Accession No.DQ341275).Conclusions Carbapenems resistance in Acinetobacter baumannii related with the production of OXA-23,PER-1 and AmpC.

The study of tumors' proteomics is a research hotspot in China and abroad in recent years.The development of theory and technology in proteomics has provided new ideas and research field for cancer research.This paper reviewed the commonly used technical means of proteomics and its research progress on diagnosis,therapy and other aspects of pancreatic carcinoma.

Objective To discuss the death causes in patients diagnosed initially as mild to moderate craniocerebral trauma. Methods A retrospective study was done on 44 deaths, who arrived at the emergency department with a mild or moderate craniocerebral trauma from 2004 to 2008. There were 35males and nine females at age range of 19-85 years (average 53 years). There were 17 patients with Glasgow coma score (GCS) 13-15 points and 27 with GCS 9-12 points. The causes of injury included road traffic accidents in 18 patients, tumbling in 18, falling from height in six and unknown factors in two respectively. Of all, 18 patients underwent surgical intervention and 26 received conservative therapy.Results The causes of death were cerebral hernia in 25 patients (57%), various complications in 15(34%), multiple trauma in two (5%) and sudden death in two (5%) respectively. Conclusions The primary death cause is cerebral hernia in the patients with mild to moderate craniocerebral trauma.Attention should be paid to prevention and control of complications and multiple trauma during treatment of primary head injury. The prognosis may be badly affected by human factors.

Objective:To explore the effect of Zinc finger protein 185 (ZNF185) on the migration of human gliocytoma cells. Methods: ZNF185 protein expression in human gliocytoma and its adjacent normal tissues was detected through Western blot. The ZNF185 gene was cloned and transfected into the human gliocytoma cell line SF767. ZNF185 cells were located through immunofluo-rescence staining, and cell migration was analyzed using the cell scoring method. Results:CZNF185 protein expression was lower in the human gliocytoma cell line than in normal para-neoplastic tissues. ZNF185 protein was mainly expressed in the cytoplasm and cell membrane of the human gliocytoma cells and was co-located with F-actin. ZNF185 overexpression repressed the migration of human gliocytoma cells. Conclusion:ZNF185 represses the migration of human gliocytoma cells by binding to F-actin.

Objective To establish the method for determining ephedrine hydochloride and pseudoephedrine hydrochloride in Keke Tablets by HPLC. Methods The samples were analyzed by a phemomenex Synergi Polar-RP column (4.6 mm×250 mm, 4 μm), with mobile phase methanol∶0.092%phosphoric acid (0.04%triethylamine and 0.02%second butylamine)=1.5∶98.5 at flow rate of 1.0 mL/min and detection at UV wavelength of 210 nm. The column temperature was 30 ℃. Results The linear ranges of ephedrine hydrochloride and pseudoephedrine hydrochloride were 6.51×10-3-0.651 μg (r=0.999 9) and 6.27×10-3-0.627 μg (r=1), respectively. The average recoveries (n=6) of ephedrine hydrochloride and pseudoephedrine hydrochloride were 102.26%and 103.71%, with RSD of 0.34%and 0.22%, respectively. Conclusion This method is simple, accurate, reproducible, highly specific and reliable results, and has the ability to effectively control the quality of alkaloids in Keke Tablets.

Objective To investigate the change rule of common coagulation indicators and platelet parameters in the patients with chronic viral hepatitis,liver cirrhosis and liver failure.Methods One hundrend and twenty patients with liver diseases in our hospital from January 2015 to June 2016 were selected and divided into chronic viral hepatitis group (46 cases),liver cirrhosis group (Child-Pugh class A,B,C,44 cases) and liver failure group (30 cases).Contemporaneous 30 persons undergoing healthy physical examination served as the healthy control group.The coagulation indicators (PT,PTA,APTT,TT, Fg,D-D) and platelet parameters (PLT,MPV,PDW, PCT) were detected in all subjects.Results Except TT,Fg and D-D had no statistical difference between the healthy control group and chronic viral hepatitis group (P>0.05),PT,APTT and TT in the chronic viral hepatitis group,liver cirrhosis group (Child-Pugh class A,B,C) and liver failure group were gradually extended,PTA and Fg were decreased and D-D value was increased,the difference in pairwise comparison was statistically significant (P<0.05).The PLT value in the chronic viral hepatitis group,liver cirrhosis group (Child-Pugh class A,B,C) and liver failure group was decreased gradually,while the MPV and PDW values were gradually increased,showing significantlystatistically significant difference (P<0.05),the PCT value in the liver cirrhosis group and liver failure group was lower than that in the chronic viral hepatitis group(P<0.05).Conclusion The regularity change of coagulation indicators and platelet parameters occurs in different periods of chronic liver disease progression,which provides some reference for clinical diagnosis,treatment and prognosis judgment.