Objective To investigate the outcome of allergen skin prick test in children with bronchial asthma,and propose prevention strategy.Methods Sensitinogen prick test on the skin was applied on 200 children who were in asthma clinic of our hospital between Mar 2007 and Oct 2010.Then,the distribution of sensitinogen and their correlation with age and sex were analysed by SPSS 17.0.Results (1) The positive rate of sensitinogen prick test in 200 children with asthma was 72.5%(145/200).The positive rate of inhalant allergen and ingestion allergen were 62.5%(125/200),14.0%(28/200),respectively,of which dermatophagoides farinae,dermatophagoides pteronyssinus,outdoor mould,mugwort,cat epidermis,tree Ⅰ,egg,milk were 36.5%,40.5%,19.0%,17.0%,17.0%,13.0%,8.0%,5.5% respectively.(2) The frequency of sensitivity to cat epidermis,mugwort,outdoor mold and tree Ⅰ increased with age and the frequency of sensitivity to egg and milk decreases with age.The sensitivity rate to all other allergens had no relation with sex.(3) The severity of sensitivity to dermatophagoides farinae,dermatophagoides,ragweed and pigweed increased with age,while severity of sensitivity had no significant relation with sex among the 18 kinds of allergens.Conclusion Dermatophagoides farinae,dermatophagoides pteronyssinus,outside mould,mugwort,tree Ⅰ,cat epidermis,egg and milk are the major allergens in children with bronchial asthma,who should avoid contact with these facters.

Objective:To develop the UPLC-MS/MS method for the determination of hesperidin, oleanolic acid, ephedrine and glycyrrhizic acid in Erchen Decoction. Methods:The separation was performed on Phenomenex Gemini C18, isocratic elution was carried out with mobile phase consisting of acetonitrile - 2 mmol/L ammonium formate. The mass spectrometer was operated in the positive and negative ionization electrospray (ESI) mode using multiple monitoring (MRM) for analysis of four components. External standard methods fix quantity. The precursor to product ion transitions monitored for hesperidin, oleanolic acid, ephedrine and glycyrrhizic acid were m/z 611.2→303.1, 455.2→407.2, 166.2→148.2 and 821.4→350.9, respectively. Results:Hesperidin, oleanolic acid, ephedrine and glycyrrhizic acid were all analyzed exactly, the linear ranges were 0.010 0-0.400 0, 0.006 3-0.250 0, 0.012 5-0.500 0, 0.002 5-0.100 0 ng, respectively. The r were 0.998 2, 0.998 8, 0.999 4 and 0.999 1, respectively. The recovery rateof the four analytes ranged from 98.45% to 102.60% and the RSD were all below 1.67%. Conclusion:This UPLC-MS/MS method is sensitive, accurate and suitable hwhich could be applied for the determination of hesperidin, oleanolic acid, ephedrine and glycyrrhizic acid in Erchen Decoction.

This paper reviews the currently research on competency of practitioners in the field of nutrition and food hygiene at home and abroad. Through literature review, it's found that the there are few researches on competency in China at present. Furthermore, compared with foreign countries, there are several issues in China, such as the methods and index system setting. However, due to different national conditions, there are significant differences in the demand for nutrition and food hygiene practitioners, related disciplines and characteristics between China and abroad. Therefore, when drawing lessons from foreign models, we should appropriately make adjustments according to China's national conditions. This review will provide references for further research on establishing a set of scientific and standardized competency evaluation indexes for practitioners in the field of nutrition and food hygiene with Chinese characteristics.

Recently,there had been many researches about the effects of brain nat-riuretic peptide(BNP)and N-terminal pro-brain natriuretic peptide(NT-proBNP)on diagnosis and differential diagnosis of cadiovascular disease,risk classification,assessment of the treatment,prognosis and screening.Some results had been applied to clinical.This article reviews the progress of BNP and NT-proBNP on pe-rioperative monitoring for congenital heart defect.

Diagnostics is the bridge from basic medicine to clinical medicine.For the students contacting the clinic for the first time,we take measures to cultivate the consciousness of showing love to patients and dedicating yourself to work.It is beneficial to cultivate the students'ability in clinical practice.

Ischemic reperfusion is the common mechanism of some chronic diseases such as type 2 diabetes,coronary heart disease,hypertension,and so on.Previous studies of insulin resistance is mainly focused on the relationships of these chronics,while now,in recent years,the research between the insulin resistance and ischemic reperfusion has gradually become one of the new and hot points,closely related to the patient's prognosis.In this paper,we will review the insulin resistance and ischemic reperfusion according to the reported literature review.

At present,in the opinions of the domestic and foreign scholars,the only hope of long-term survival even cure of hilar cholangiocarcinoma was obtained by radical resection(ie R0 resection).The application of preoperative assessment with appropriate and comprehensive strategies can judge the respectability and Intraoperative safety accurately to the tumor,thereby it can improve the radical resection rate and safety of patients with hilar cholangiocarcinoma.In this paper,preoperative assessment strategies are reviewed.

For the patients with early non-small cell lung cancer (NSCLC) who can tolerate surgery,surgery remains the standard treatment.For that patients who can not tolerate surgery,a large number of studies have shown that stereotactic body radiothery (SBRT) plays an indispensable role in the treatment of early NSCLC.

Objective Analysing the potential relationship of mismatch repair protein MLH3 gene C2531T mutation with oligospermia and azoospermia cases. Methods Using MLH3 C2531T as an example to establish a method of tetra-primer amplification refractory mutation system-PCR (4P-ARMS-PCR); We chose 81 cases of oligospermia, azoospermia patients and 80 normal controls, detecting MLH3 C2531T both using 4P-ARMS-PCR and PCR-RFLP; randomly to select 20% samples to sequence. Results The results suggested higher prevalence of CT + TT genotypes in patients as compared to the controls and association of T allele with disease (P <0. 01); PCR-RFLP results , 4P-ARMS-PCR results and sequence results are fully consistent. Conclusion 4P-ARMS-PCR can be a fast, easy-operating and accurate technology for detection of gene mutation of single nucleotide polymorphism (SNP) ; MLH3 C2531T polymorphism is related to oligospermia and azoospermia.

Autophagy is the cell biology process in which cytoplasmic components are degraded in lysosomes to maintain cellular homeostasis,it is involved in the pathophysiology of several kidney diseases (podocytopathies,IgA nephropathy,diabetic nephropathy,hereditary kidney disease),but its specific effect is still controversial.Most studies suggest that autophagy plays a protective role in kidney diseases,in the future,it may become a new target for the prevention and treatment of kidney disease.