Objective To explore the effect of aspirin resistance on cardiovascular events in non-insulin-dependent diabetes melli-tus patients .Methods 496 patients with non-insulin-dependent diabetes mellitus received daily aspirin therapy (100mg/d) 4 weeks were recruited from Jun .2006 to Sept .2010 .Platelet aggregation was measured by light transmission aggregometry .The patients were divided into three groups :aspirin resistance group ,aspirin semi-resistance group and aspirin-sensitive group .The clinical bio-chemistry indications and the adverse cardiovascular events were collected .Results 11 .69% of patients were found to be resistant to aspirin therapy ,and 37 .90% of patients to be aspirin semi-responders .There were 67 .48% female in aspirin resistance group and aspirin semi-responders .The preprandial glucose of group AR and ASR was higher than that of group AS (P<0 .05) .The HbA1C and TG of group AR were higher than those of group AS(P<0 .05) .There was statistically significant difference of the rate of car-diovascular events .Conclusion The measure of platelet aggregation should be taken in the diabetic mellitus patients and pay atten-tion on the dose of aspirin recommended .Particular attention is should also devote to the incidence of adverse cardiovascular events .

ObjectiveTo identify the proteins presented variation during ischemia/reperfusion injury (I/RI) in liver transplantation (LT).MethodsAt Mar.2009,a total of nine liver sample tissues,three samples obtained from each donor liver at three different time points,were studied.The time points were as follows:(1) the time point immediately after donor liver was harvested (T1) ; (2) the time point just before the anastomosis of hepatic artery and potal vein (T2),during this time period the donor liver was kept in ice cold and transported,then back-table prepared,during time from T1 to T2,ischemia injury occurred; (3) the time after donor reconnected to the recipient (T3),during which the donor liver was subjected to reperfusion injury.The three time points represent normal control,ischemia injury,and reperfusion injury respectively.By using method of 2DE-MALDI-TOF and mass-spectrum,the differential expression of these protein at T1,T2,and T3 time point were compared.ResultsFrom the 1580 proteins that were isolated,19 ischemia/reperfusion injury (I/RI)-related proteins that varied markedly from time point T1 to T2 and from time point T2 to T3 were identified.They were metabolic enzyme,molecular chaperone,redoxase,cytoskeleton protein,signal transduction protein,and binding protein,respectively.The character of each protein was further analized based on their functions.ConclusionsFor the first time,we detected the protein related to I/RI during LT.We obtained independent proteomics information of ischemia injury,and reperfusion injury,respectively.Further functional analyses of these proteins may improve the understanding of the critical biological processes during I/RI.Medicine intervention on the I/RI-related proteins may result in improved survival of the recipients.

BACKGROUND: The filling compactness can not be guaranteed due to apparatus retention in traditional lateral condensation.The warm gutter filling can achieve good results because of the easy changeable of gutta-percha in softening states.OBJECTIVE: To compare the warm gutta-percha fillings (Obtura Ⅱ and BeeFill 2 in1 filling) and cold lateral compacted gutta-percha with the bypassing root canal preparation in the intracanal separated instrument of oval canal.METHODS: Firstlythe 60 oval root canals of fiat mandibular central incisors were randomly divided into three groups, with 20 teeth in each group. After that, the root canal was filled with BeeFill 2 in1, Obtura Ⅱ condensation, and lateral condensation.The percentage of void at 5-mm to root tip versus cress section area was observed under a scanning electron microscope.RESULTS AND CONCLUSION: Under in vitro condition, the difference of percentage of void area versus root canal area between the Obtura Ⅱ condensation group and the lateral condensation group had significance (P=0.013), and the similar difference could be found between the BeeFill 2 in1 condensation group and the lateral condensation group (P=0.014), but the difference had no significance between the BeeFill 2 in1 condensation group and the Obtura Ⅱ condensation group (P=0.088).The results revealed that the root canal can be sealed accurately when establish hypass root next to the separated instrument and filled with BeeFill 2 in1 warm gutter filling.

Food allergy( FA) is a growing health problem which affects young children′s growth. Re-cent studies have showed that leukotrienes can contract gastrointestinal smooth muscle,increase intestinal perm-bility,and it is obviously related to the development of FA gastrointestinal disorder. Leukotriene receptor antago-nists have provided a good therapeutic option and showed clinical benefits in the management of the FA gastroin-testinal disorder.

Neurally ajd usted ventilatory assist( NAVA) uses the electrical activity of the diaphragm as a neural trigger to synchronize mechanical ventilatory breaths with the patient′s neural respiratory drive. Some studies have shown that compared with pressure-support ventilation,NAVA can improve patient-venti-lator synchrony while maintaining spontaneous breathing,unload respiratory musculaturei n term adn prte erm i nfants.Further studies are needed to determ ine whether NA VA will have significant impact on the overall outcomes of neonates.

Objective To establish animal tumor model expressing human oncogene HER2/neu and to evaluate the model by immunological method. Methods The recombinant eukaryotic plasmid expressing HER2/neu gene was transfected into P815 tumor cells. The tumor cell line P815 expressing HER2/neu gene stably was screened by limiting dilution method. The expression of target gene in host cells was determined by Western blotting. Following the establishment of animal tumor model expressing HER2 antigen in DBA/2 mice, tumor specific response was induced in the spleen cells of DBA/2 mice which were inoculated with recombinant plasmids. Results P815 cell line expressing HER2/neu oncogene stably was obtained. HER2/neu specific CTLs could be induced from immunized DBA/2 mice. Conclusion HER2/neu expressing tumor animal model is established successfully and the in vivo immune response can be induced after immunization with HER2/neu genetic vaccine.

Objective To study the clinical diagnosis,treatment and prognosis of and the factors influencing prognosis of breast cancer(BC) in young women. Methods A restrospective analysis of the clinical data of 59 cases treated from Jan .1990 to Dec. 1998was carried out in the Second Xiangya Hospital. Results All patients underwent operation, including 31 radical mastectomy , 25 modified radical mastectomy and 3 simple mastectomy. 34 cases had axillary lymph node metastasis. The overall 3,5 and 7 year survival rates were 67.2% , 61.0% and 57.1%respectively. The 3,5 year survival rates in patients with axillary-lymph node metastasis were lower than those in patients without axillary lymph node metastasis (61.8%,45.5%vs 86.4% ,83.3%). The 3,5 year survival rates in patients with negative C erbB 2 expression were 84.6%,82.4% and in patients with positive C erbB 2 expression were 54.6%,45.8%,respectively(all P

Objective:To explore the reading feature of developmental dyslexia. Methods:820 children of grade 3~5 in 5 primary schools in Wuhan City were selected by cluster sampling; fifty-five children with developmental dyslexia were screened out according to ICD-10. One hundred and ten normal children with the same age, sex and socioeconomic status were chose as control. Results:The prevalence rate of developmental dyslexia is 6.7%. The score of 8 items of DCCC (Dyslexia Checklist for Chinese Children) of developmental dyslexics were mostly significantly higher than that of normal children (15.2?3.7/10.1?3.3, 21.6?3.7/13.4?4.9, 16.1?3.5/10.6?3.7, 16.8?3.6/11.7?3.1, 15.9?3.9/11.5?3.5, 21.4?4.3/14.5?4.6, 14.9?3.7/9.4?2.8, 23.2?4.4/15.9?4.7, all withP

There are some unsafe and inconvenient problems in moving Ⅴb-type field tents because the overall weight amounts to 310Kg,and the medical boxes are lack of sign cards and block slots.Improving methods and suggestions for these problems are put forword.

Study on proteomics can explain the protein biological function of executing life activities expressed by genome.The results of the research point to a new direction for pharmaceutical and clinical development.This review focuses on the latest developments of the qualitative and guantitative researches on biological mass spectrometry in proteomics,as well as their advantages and prospects.