Objective To investigate the CT and MRI manifestations and clinical features of liver fluke granuloma.Methods A retrospective analysis was performed for the clinical and imaging data of 5 patients with pathologically confirmed liver fluke granuloma who were hospitalized in The First Affiliated Hospital of Guangxi Medical University from January 2010 to September 2015.Results Liver fluke granuloma had slightly low density on CT plain scan,as well as a slightly low signal on T1 weighted images and a slightly higher signal on T2 weighted images of MRI plain scan.Three-phase contrast-enhanced CT scan showed delayed enhancement with mild dilatation of the intrahepatic bile duct,and normal vessels ran through the lesion.Conclusion Liver fluke granuloma is a rare disease in chnical practice.A history of eating raw fish,delayed enhancement on three-phase contrast-enhanced CT scan,and normal vessels running through the lesion all contribute to the diagnosis of liver fluke granulomas.

Objective:Human metapneumovirus (hMPV),initially described in 2001,is an enveloped RNA virus of the genus Metapneumovirus,subfamily Pneumovirinae,family Paramyxoviridae. Here we sought to clarify basic features of human metapneumovirus proteins. Methods:Rabbits were immunized with inactivated virons of hMPV Chinese first isolate,CHN05-01,to yield anti-hMPV antiserum. Antiserum was used as primary antibody to detect hMPV proteins by Western blotting. NetNglyc1.0 server,NetOglyc 3.1server and the NetPhos 2.0 server were applied for predicting potential glycosylation and phosphorylation sites of proteins of prototype virus of type A,CAN97-83. Results:The highest reactive titer of the antiserum with hMPV antigens reached 1:500 in ELISA. Potential glycosylation sites of G protein and phosphorylation sites of P protein were greatest among all hMPV proteins. G protein was shown a narrow band with molecular weight between 55 and 72kDa (approximately 68kDa),indicating its glycosylation level being consistent and remarkably different from that of CAN99-80 and CAN99-81. F1 subunit of fusion protein displayed molecular weight between 40 and 55kDa (approximately 48 kDa),which is consistent with previous reports. Conclusion:Basic features of two major membrane proteins of Chinese human metapneumovirus isolate were clarified,which will benefit future studies on protein funtion and pathogenesis of this virus.

Objective To investigate the clinicopathological characteristics and treatment of nodular lymphocyte-predominant Hodgkin lymphoma (NLPHL). Methods Seven patients were confirmed as NLPHL by pathologic immunohistochemistry. Six patients received combined-modality therapy of chemotherapy and involved field, and the other one received single chemotherapy. Results The 3-year local control rate and overall survival rate were 100 % and 86 %, respectively. Only one case died of pulmonary infection following chemotherapy. Conclusion These patients with NLPHL has favorable prognosis, tolerance and less toxicity for combined-modality therapy. However the management of toxicity following treatment should be noted.

Objective To investigate the effect of tourniquet modified strapping on venipuncture in tumor patients.Methods 120 tumor patients whose veins were exposed hard in radiotherapy department from October 2016 to March 2017, randomly divided into the control group and the observation group equally.The control group was treated with the traditional single strapping tourniquet to have venipuncture and the observation group with tourniquet modified strapping.Results The venous filling degree, the success rate of first puncture, and the satisfaction of the patients in the observation group were significantly higher than those in the control group (P<0.05).Conclusion The use of tourniquet modified strapping in tumor patients whose veins were exposed hard can significantly improve the venous filling degree, the success rate of puncture, the satisfaction of patients and the quality of nursing service, and thus it is worthy of clinical promotion.

Objective To investigate the clinical efficacy and mechanism of minocycline hydrochloride ointment adjuvant therapy for chronic periodontitis.Methods86 patients of chronic periodontitis who received therapy from August 2013 to June 2015 in our hospital were selected.According to random number table, those patients were divided into the experimental group (n=43) and the control group (n=43).The control group was treated on the basis of the foundation treatment with tinidazole Oral Sticking Tablets and the experimental group were treated on the base of the foundation treatment with Minocycline hydrochloride sustained-release ointment, the levels of hs-CRP, TNF-a, IL-8 and IL-10 in gingival sulcus fluid were measured before and after treatment, the indexes of PLI, PD, SBI and AL were recorded,at the same time the clinical curative effect between two groups was compared.ResultsThe effective rate of the experimental group(95.35%) was higher than the control group(81.40%),the difference was statistically significant (P<0.05);Compared with before treatment, two groups general situations and inflammation factors changed after treatment,plaque index, periodontal pocket probing depth, gingival sulcus bleeding index and adhesion levels of the experimental group were lower than the control group after treatment, the PD, SBI, AL and PLI in the experimental group were significantly lower than that of the control group, and the hs-CRP, TNF-a and IL-8 levels were lower than the control group, IL-10 was higher than the control group after treatment(P<0.05).ConclusionMinocycline hydrochloride ointment is well for chronic periodontitis,which can effectively relieve periodontal chronic inflammation, improve clinical efficacy, the underlying mechanism may be related to the reduction of IL-8 and TNF-α levels in gingival crevicular fluid.

Objective To explore the application of mesangial thyroid resection in papillary thyroid carcinoma(PTC) with central lymph node dissection,and its influence on postoperative recurrence.Methods 76 cases with PTC were divided into two groups according to random number table,each group in 38cases.The control group was given primary lesions radical prostatectomy and routine central lymph node dissection,the treatment group was given primary lesions radical prostatectomy and central lymph node dissection by thyroid mesangial resection.The parathyroid function,injury of recurrent laryngeal nerve and local recurrence were compared between the two groups.Results Compared with before surgery,the levels of serum PTH and blood calcium were significantly decreased at the 1st,3rd day after surgery (all P ＜ 0.05),while there were no statistically significant differences in serum PTH and blood calcium at the 7th day after surgery(all P ＞0.05).The levels of serum PTH and blood calcium at the lst,3rd,7th day after surgery between the two groups had no statistically significant differences(all P ＜ 0.05).The incidence rate of injury of recurrent laryngeal nerve in the treatment group was significantly lower than that in the control group (5.3％ vs.21.1％,x2 =4.15,P ＜ 0.05).The secondary surgery rate and local recurrence within 2 years in the treatment group were significantly lower than those in the control group (0％ vs.15.8％,2.6％ vs.18.4％,x2 =6.51,4.69,all P ＜ 0.05).Conclusion Primary lesions radical prostatectomy and central lymph node dissection by thyroid mesangial resection can be used as theroutine operation for PTC,which can effectively produce complete dissection,reduce injury of recurrent laryngeal nerve and local recurrence.

corrected visual acuity and good visual quality are clinic effects standard of laser in situ keratomeleusis.Visual quality descending for patients after LASIK surgery depends on many factors.This review recommends analyses the influence of visual quality for patients after LASIK surgery and it's guiding significance to LASIK from seven sides,such as visual acuity,refactive diopter,contrast sensitivity,wavefront aberration,increment of Q-value,binocular visual function,the change of dominant eye.

OBJECTIVE: Nuclear factor (NF)-?B is an inducible transcription factor detected in neurons, glial and neural stem cells. It is involved in many biological processes such as inflammation and innate immunity, development, apoptosis and anti-apoptosis. This text is aimed to explain the roles of NF-?B in proliferation, migration and differentiation of neural stem cells. DATA SOURCES: The related literatures between January 1987 and September 2006 were collected from the PUBMED with the keywords of "Neural stem cell, Proliferation, Migration, Differentiation/stem cell, Differentiation/NF-?B" in English. STUDY SELECTION: Firstly the articles were checked and quotations attached to these articles were looked over. Inclusive criteria: the content of the articles must be correlated with roles of NF-?B in proliferation, migration, differentiation of neural stem cell. Exclusive criteria: Repetitive research or articles of Meta analysis. DATA EXTRACTION: There were 61 correlated articles, 32 articles accorded with inclusive criteria, and 29 articles excluded were olden or repetitive in content. In 32 collected articles, 4 articles referred to adult neural stem cell, 3 articles referred to NF-?B/Rel family and Ⅰ-?B, 11 articles referred to NF-?B and proliferation of neural stem cell, 8 articles referred to NF-?B and migration of neural stem cell and 6 articles referred to NF-?B and differentiation of neural stem cell. DATA SYNTHESIS: Neural stem cells were characterized by the capability to undergo self-renewal, cell divisions, to migrate and to differentiate into multiple cell types. Stem cells within the adult brain were found within special areas. Neural stem cells experienced the processes of dissociation, immigration and differentiation. NF-?B was a transcription factor, which can bind to specific DNA-sequences and regulate transcription. NF-?B was involved in induction of proliferation under erythropoietin (EPO), hypoxia conditions and brain injury. The inhibitors of NF-?B such as beta-peptide, nitric oxide acted in negative manner on proliferation of neural stem cells. In migration of neural stem cell, NF-?B was necessary for induction of migration caused by macrophage chemo attractant protein-1, stem cell factor, stromal cell derived factor. The differentiation of neural stem cells toward astrocytic, neuronal, oligodendrocytic and glial lineages was determined by specific signaling cascades. Interleukin-6 family could accelerate the differentiation of neural stem cells to horizontal cells. The differentiation of neuroblastoma cells could be joined by NF-?B. CONCLUSION: A great quantity of evidences verifies that NF-?B plays a crucial role in the complicated regulatory mechanism of transcription of neural stem cells.

OBJECTIVE:To investigate the effects of Danhong injection combined with low molecular weight heparin (LM-WH) on clinical symptoms,serum inflammatory factors and lung function in elderly patients with chronic obstructive pulmonary disease (COPD). METHODS:A total of 146 elderly patients with COPD were randomly divided into observation group and con-trol group,with 73 cases in each group. Both groups received conventional treatment. On this basis,control group was given Dan-hong injection 30 mL,intravenous drip,once a day. Observation group was additionally given LMWH sodium injection 5000 IU, subcutaneously,twice a day,on the basis of control group. Both groups were treated for 14 d. Clinical efficacies of 2 groups were observed and compared. The clinical symptom score,the levels of IL-6,IL-8,TNF-α,FEV1,FEV1/FVC,pa(O2),pa(CO2) and the occurrence of ADR were compared before and after treatment. RESULTS:After treatment,the total response rate of observa-tion group was significantly higher than that of control group(93.15%vs. 78.08%,P<0.05). Before treatment,there was no statis-tical significance between above indexes (P>0.05). After treatment,individual clinical symptom score,total clinical symptom score,the levels of IL-6,IL-8,TNF-α,pa(CO2)in 2 groups were significantly lower than before;the observation group was signif-icantly lower than the control group;FEV1,FEVl/FVC,pa(O2) were significantly higher than before,and the observation group was significantly higher than the control group,with statistical significance (P<0.05). CONCLUSIONS:Based on conventional treatment,Danhong injection combined with LMWH is effective in the treatment of senile COPD,and can significantly improve the clinical symptoms,serum inflammatory factors levels and pulmonary function with good safety.

Objective To investigate the effect of noninvasive positive pressure ventilation NIPPV in treatment of chronic obstructive pulmonary disease with acute exacerbation of respiratory failure. Methods From April 2014 to January 2015, 80 patients with chronic obstructive pulmonary disease and acute exacerbation of respiratory failure in our hospital were enrolled in this study. The patients received anti-infectious, antispasmodic, expectorant, correct electrolyte imbalance and symptomatic and supportive cure. BiPAP Symchrony ventilator was applied in the patients in the treatment group, and low flow nasal catheter ventilation therapy was given to patients in the control group. The following parameters were observed and compared: (1)The clinical efficacy of two groups of patients; (2) RR, PaO2, PaCO2, pH changes of patients before and at 2, 24 hours after ventilation therapy. (3) Intubation and mechanical ventilation, mortality, average length of stay and complications that occur during treatment. Results After treatment, the total effective rate of patients in the treatment group was significantly higher than that of patients in the control group (P < 0.05). At the end of treatment, RR, PaO2, PaCO2, pH changes, aeration at 2 h, 24 h post ventilation of patients in the treatment group improved significantly (P<0.05). The intubation and mechanical ventilation rate, mean hospital stay of patients in the treatment group were significantly shorter than theose of patients in the control group (P<0.05). Conclusion To patients with chronic obstructive pulmonary disease and acute exacerbation of respiratory failure, NIPPV can improve clinical outcomes, improve the patient′s oxygen partial pressure and oxygen saturation, shorter hospital stays, inhibit the rates of mechanical ventilation.