Objective To investigate the CT and MRI manifestations and clinical features of liver fluke granuloma.Methods A retrospective analysis was performed for the clinical and imaging data of 5 patients with pathologically confirmed liver fluke granuloma who were hospitalized in The First Affiliated Hospital of Guangxi Medical University from January 2010 to September 2015.Results Liver fluke granuloma had slightly low density on CT plain scan,as well as a slightly low signal on T1 weighted images and a slightly higher signal on T2 weighted images of MRI plain scan.Three-phase contrast-enhanced CT scan showed delayed enhancement with mild dilatation of the intrahepatic bile duct,and normal vessels ran through the lesion.Conclusion Liver fluke granuloma is a rare disease in chnical practice.A history of eating raw fish,delayed enhancement on three-phase contrast-enhanced CT scan,and normal vessels running through the lesion all contribute to the diagnosis of liver fluke granulomas.

Objective:Human metapneumovirus (hMPV),initially described in 2001,is an enveloped RNA virus of the genus Metapneumovirus,subfamily Pneumovirinae,family Paramyxoviridae. Here we sought to clarify basic features of human metapneumovirus proteins. Methods:Rabbits were immunized with inactivated virons of hMPV Chinese first isolate,CHN05-01,to yield anti-hMPV antiserum. Antiserum was used as primary antibody to detect hMPV proteins by Western blotting. NetNglyc1.0 server,NetOglyc 3.1server and the NetPhos 2.0 server were applied for predicting potential glycosylation and phosphorylation sites of proteins of prototype virus of type A,CAN97-83. Results:The highest reactive titer of the antiserum with hMPV antigens reached 1:500 in ELISA. Potential glycosylation sites of G protein and phosphorylation sites of P protein were greatest among all hMPV proteins. G protein was shown a narrow band with molecular weight between 55 and 72kDa (approximately 68kDa),indicating its glycosylation level being consistent and remarkably different from that of CAN99-80 and CAN99-81. F1 subunit of fusion protein displayed molecular weight between 40 and 55kDa (approximately 48 kDa),which is consistent with previous reports. Conclusion:Basic features of two major membrane proteins of Chinese human metapneumovirus isolate were clarified,which will benefit future studies on protein funtion and pathogenesis of this virus.

Objective To investigate the clinicopathological characteristics and treatment of nodular lymphocyte-predominant Hodgkin lymphoma (NLPHL). Methods Seven patients were confirmed as NLPHL by pathologic immunohistochemistry. Six patients received combined-modality therapy of chemotherapy and involved field, and the other one received single chemotherapy. Results The 3-year local control rate and overall survival rate were 100 % and 86 %, respectively. Only one case died of pulmonary infection following chemotherapy. Conclusion These patients with NLPHL has favorable prognosis, tolerance and less toxicity for combined-modality therapy. However the management of toxicity following treatment should be noted.

Objective To investigate the effects of absorbable gelatin sponge, chitosan and sodium hyaluronate(HA) on inhibition of postoperatively epidural scar formation in animal models with posterior discectomy and the concentrations of HA in the cerebral spinal fluid was also determined. Methods 96 Wistar rats older than 12 months were equally allocated into four groups. Each animal underwent a unilateral hemi-laminectomy and discectomy. Absorbable gelatin sponge, chitosan and HA were placed at the sites of surgery in three groups respectively. The fourth group undergone laminectomy and discectomy only, was served as control. At the time of 2,4, 8, 12 weeks postoperatively, 6 rats in each group were sacrificed for gross evaluation, histology and transmission electron microscope studies. The areas of epidural scar and spinal canal were measured by a computer image processor, and were compared statistically. The concentrations of HA in the cerebral spinal fluid in the HA groups and control groups were tested. Results The epidural scar areas in the chitosan and HA groups were significantly less than those in the control and absorbable gelatin sponge groups; no significant difference was found among the spinal canal areas of all the groups at different time phase; the compression and dislocation of the dura and spinal nerve roots and the discontinuity of the annulus fiber were observed in every group and time phase. The two cases with highest concentrations of HA were in the HA group at 2-week. Conclusion Chitosan and HA are able to inhibit the epidural scar formation after posterior discectomy. The healing of the annulus must be considered when evaluating the scar inhibition effects of materials. When the materials are placed in the epidural space, their effects on the central nervous system should be considered.

Programmed cell death(PCD)of human leukemic HL-60 cell and human poorly differentiated gastric adenocarcinoma cell BGS-180 induced by efoposid(VP-16) was preliminarily observed comparatively in the same experimental condition through rate of cell death, DNA agarose gels electrophoresis and terminal deoxynucleotidyl transferase(TdT) mediated biotin-11-dUTP nickend labeling(TUNEL).It was found that apoptosis is the main pattern of HL-60 cell death induced by low does VP-16 in a short time, and it can be suppressed when protein kinase C(PKC) is activated. The main pattern of BGS-180 cell death induced by VP-16 is necrosis, and PCK activation does not affect its necrosis rate. Extracellular Ca2+ reduction do not affect BGS-180 and HL-60 cell death rate. The mechanism of VP-16 action on BGS-180 and HL-60 cell is different, apoptosis is not the main pattern of cell BGS-180 death induced by VP-16.

Etiologic classification of ischemic stroke is the basis for epidemiological investigation, risk factor screening developing individualized treatment plan and prognostic measures. The etiologic classifications, such as TOAST, CCS, and ASCO, are more generally accepted methods at present. This article reviews the advantages and disadvantages from the diagnostic criteria and clinical application.

Objective:To study the antigenicity of OMP extracted from Edwardsiella tarda.Methods:ELISA, Bactericidal test, Agglutinating test and Western blotting were used for testing the antigenic titers and immunogenicity of OMP.Results:In immunoblotting, by using ATCC15947 OMP antibody, the non pathgenic strains were negative, while all pathogenic strains except Et 122 gave positive results and had OMP bands of 33k, 35k, 38k, and 45k. OMPs of both ATCC 15947 and JEL4 could induce high antibody titers. Further more, the antibodies evoked by OMPs of ATCC 15947 of 33k or 35k could also protected mice to some degree when diluted.Conclusion:The 33k, 35k, 38k, and 45k of OMPs may be protective antigens, and the OMPs of Et could be a candidative component for vaccine.

Objective To investigate the clinical efficacy and mechanism of minocycline hydrochloride ointment adjuvant therapy for chronic periodontitis.Methods86 patients of chronic periodontitis who received therapy from August 2013 to June 2015 in our hospital were selected.According to random number table, those patients were divided into the experimental group (n=43) and the control group (n=43).The control group was treated on the basis of the foundation treatment with tinidazole Oral Sticking Tablets and the experimental group were treated on the base of the foundation treatment with Minocycline hydrochloride sustained-release ointment, the levels of hs-CRP, TNF-a, IL-8 and IL-10 in gingival sulcus fluid were measured before and after treatment, the indexes of PLI, PD, SBI and AL were recorded,at the same time the clinical curative effect between two groups was compared.ResultsThe effective rate of the experimental group(95.35%) was higher than the control group(81.40%),the difference was statistically significant (P<0.05);Compared with before treatment, two groups general situations and inflammation factors changed after treatment,plaque index, periodontal pocket probing depth, gingival sulcus bleeding index and adhesion levels of the experimental group were lower than the control group after treatment, the PD, SBI, AL and PLI in the experimental group were significantly lower than that of the control group, and the hs-CRP, TNF-a and IL-8 levels were lower than the control group, IL-10 was higher than the control group after treatment(P<0.05).ConclusionMinocycline hydrochloride ointment is well for chronic periodontitis,which can effectively relieve periodontal chronic inflammation, improve clinical efficacy, the underlying mechanism may be related to the reduction of IL-8 and TNF-α levels in gingival crevicular fluid.

Objective To explore the application of mesangial thyroid resection in papillary thyroid carcinoma(PTC) with central lymph node dissection,and its influence on postoperative recurrence.Methods 76 cases with PTC were divided into two groups according to random number table,each group in 38cases.The control group was given primary lesions radical prostatectomy and routine central lymph node dissection,the treatment group was given primary lesions radical prostatectomy and central lymph node dissection by thyroid mesangial resection.The parathyroid function,injury of recurrent laryngeal nerve and local recurrence were compared between the two groups.Results Compared with before surgery,the levels of serum PTH and blood calcium were significantly decreased at the 1st,3rd day after surgery (all P ＜ 0.05),while there were no statistically significant differences in serum PTH and blood calcium at the 7th day after surgery(all P ＞0.05).The levels of serum PTH and blood calcium at the lst,3rd,7th day after surgery between the two groups had no statistically significant differences(all P ＜ 0.05).The incidence rate of injury of recurrent laryngeal nerve in the treatment group was significantly lower than that in the control group (5.3％ vs.21.1％,x2 =4.15,P ＜ 0.05).The secondary surgery rate and local recurrence within 2 years in the treatment group were significantly lower than those in the control group (0％ vs.15.8％,2.6％ vs.18.4％,x2 =6.51,4.69,all P ＜ 0.05).Conclusion Primary lesions radical prostatectomy and central lymph node dissection by thyroid mesangial resection can be used as theroutine operation for PTC,which can effectively produce complete dissection,reduce injury of recurrent laryngeal nerve and local recurrence.

OBJECTIVE:To investigate the effects of Danhong injection combined with low molecular weight heparin (LM-WH) on clinical symptoms,serum inflammatory factors and lung function in elderly patients with chronic obstructive pulmonary disease (COPD). METHODS:A total of 146 elderly patients with COPD were randomly divided into observation group and con-trol group,with 73 cases in each group. Both groups received conventional treatment. On this basis,control group was given Dan-hong injection 30 mL,intravenous drip,once a day. Observation group was additionally given LMWH sodium injection 5000 IU, subcutaneously,twice a day,on the basis of control group. Both groups were treated for 14 d. Clinical efficacies of 2 groups were observed and compared. The clinical symptom score,the levels of IL-6,IL-8,TNF-α,FEV1,FEV1/FVC,pa(O2),pa(CO2) and the occurrence of ADR were compared before and after treatment. RESULTS:After treatment,the total response rate of observa-tion group was significantly higher than that of control group(93.15%vs. 78.08%,P<0.05). Before treatment,there was no statis-tical significance between above indexes (P>0.05). After treatment,individual clinical symptom score,total clinical symptom score,the levels of IL-6,IL-8,TNF-α,pa(CO2)in 2 groups were significantly lower than before;the observation group was signif-icantly lower than the control group;FEV1,FEVl/FVC,pa(O2) were significantly higher than before,and the observation group was significantly higher than the control group,with statistical significance (P<0.05). CONCLUSIONS:Based on conventional treatment,Danhong injection combined with LMWH is effective in the treatment of senile COPD,and can significantly improve the clinical symptoms,serum inflammatory factors levels and pulmonary function with good safety.