Humans ; Lead ; Spectrometry, Fluorescence ; Spectrophotometry, Atomic ; Uncertainty

ObjectiveTo investigate the action mechanism of Th17 cells in immunoinflammatory response in systemic lupus erythematosus(SLE).MethodsReverse-transcription PCR was performed to measure the mRNA expre ssion of the retinoic acid receptor-related orphan nuclear receptor RORγt in peripheral blood mononuclear cells (PBMCs) from 12 patients with active SLE,9 patients with inactive SLE and 12 normal human controls.Data were statistically analyzed by approximate F test(Welch test) and Dunnett's T3 multiple comparison test (corrected).ResultsIn the case of RORγt mRNA expression in PBMCs,significant differences existed among the 3 groups(F =23.286,P ＜ 0.01 ); in detail,the patients with active SLE were significantly higher than patients with inactive SLE and normal controls ( 1.06 ± 0.44 vs.0.65 ± 0.25,F =2.453,P ＜ 0.05;1.06 ± 0.44 vs.0.22 ± 0.08,F =6.504,P ＜ 0.05),and the patients with inactive SLE were significantly increased compared with the normal controls (F =3.343,P ＜ 0.05).The expression level of RORγt mRN A was significantly positively correlated with SLE disease activity index (rp =0.623,P ＜ 0.01 ).ConclusionsThere is a polarization of Th17 cells in patients with SLE.To antagonize the transcription factor RORγt,which plays an essential role in the regulation of Th17 cell differentiation,may facilitate the control of SLE via attenuating the immunoinflammatory response.

Se load tests were carried out by administering 150?g Se as 0.1% sodium selenite 0. 5ml in 5 groups of male children (7～13 years) including 2 groups from different Kashin-Beck disease affected areas, 2 groups from non-endemic areas and another from an endemic area supplementing Se-fortilled salt for 4 years. The results showed that Se urinary excretion amounts, 6,12 and 24 hour accumulated rates and 24 hour net excretion amounts as well as rates after Se administration were similar between 2 endemic groups, but all far lower than those of 2 non-endemic groups; that urinary Se excretion peak in endemic groups delayed as comparied with non-endemic groups and that the above—mentioned parameters in endemic group supplementing Se restored to the levels of non-endemic groups. All those findings suggested that the children from Kashin-Beck disease affected areas might be in a Se deficiency status and Se supplementation to the endemic children consuming Se fortified salt significantly improve their Se deficiency status.

Objective To explore the expression of Hes1 mRNA during neural stem cells(NSC) differentiation toward neurons . Methods To establish the model of cultivation NSC in the hippocampal of newborn (24 h) SD rats ,and then to observe the mor-phology of NSC in the course of proliferation and differentiation .Before and after cellular induction ,the expression of Nestin and NSE were respectively measured to detect cell types by immunochemistry method .And flow cytometry was used to determine cell cycle phases ,so as to detect proliferative activity of these cells .Meanwhile ,the expression of Hes1 mRNA in NSC was determined by reverse transcription-PCR(RT-PCR) .Results The results demonstrated that NSC isolated from hippocampal showed vigorously clonal proliferation in vitro ,and positive Nestin expression .In addition ,the differentiated cells demonstrated positive NSE expres-sion .Flow cytometry analysis showed that the percentage of NSC in S phase was obviously higher than that of induced differentia-tion of all time(P<0 .01) ,which indicated that NSC were actively dividing induction before .Compared to NSC ,the percentages of cells in G0 G1 phases were increased significantly after neuronal differentiation (P<0 .01) ,which indicated that differented cells have arrested in G0 G1 phases .Meanwhile ,the results from RT-PCR showed that :Hes1 mRNA was expressed in NSC both before and af-ter induced differention .Compared to induction before ,the level of Hes1 mRNA expression in NSC during different stages of differ-entiation after induction were significantly decreased (P<0 .05) ,and Hes1 mRNA did not show any obvious changes among these stages of differentiation(P>0 .05) .Conclusion The high level of Hes1 mRNA was probably involved proliferation of NSC .How-ever ,low level of Hes1 mRNA might contribute to neuronal differentiation .

Objective: To investigate the expression of Wnt/β-catenin pathway in diabetic nephropathy (DN) rats and the intervention effect of Chinese materia medica (CMM) for dispersing blood stasis and dredging collateral. Methods: Ten rats were selected as control group from 60 rats, the remaining rats were established as DN models by feeding high glucose and high fat diet combined with low-dose streptozotocin ip injection. Model rats were randomly divided into model group, irbesartan treatment group, and CMM group. The rats in each group were ig administered with corresponding drug, at the end of the 20th week, the 24 h urinary total protein was detected. The expression levels of Wnt4 and β-catenin mRNA and protein in renal tissue were detected. Results: Compared with control group, the 24 h urinary total protein, expression of Wnt4, β-catenin mRNA, and protein significantly increased in the model group (P < 0.01). Compared with model group, 24 h urinary total protein, the expression of Wnt4, β-catenin mRNA, and protein decreased significantly in irbesartan group and CMM group (P < 0.01 or P < 0.05). Conclusion: CMM for dispersing blood stasis and dredging collateral might decrease proteinuria in DN rats. It can also inhibit the high expression of Wnt/β-catenin pathway in the kidney of diabetic nephropathy rats. The effect might be one of the main ways to reduce urinary protein excretion.

ObjectiveTo explore the effective methods to improve the pulmonary function and life quality of patients with idiopathic pulmonary fibrosis (IPF).Methods48 IPF patients were randomly divided into two groups, each group includes 24 cases. Group A was treated with prednisone 0.5 mg/kg per day orally, reduce half of the dosage 4 weeks later and Fluimucil 600 mg three times per day orally, while group B with prednisone 0.5 mg/kg per day orally, reduce half of the dosage 4 weeks later. The clinical manifestation, pulmonary function testing, high-resolution CT of the chest, arterial blood gas analysis were observed before and 3 months after treatment. ResultsThere was significant difference in Group A (P<0.05) but no significant difference in Group B (P>0.05) before and after treatment in the pulmonary function testing and arterial blood gas analysis. There was significant difference after treatment in the pulmonary function testing and arterial blood gas analysis between Group A and Group B (P<0.05).There was significant difference after treatment in the clinical manifestation, high-resolution CT of the chest and between Group A and Group B (P<0.05). ConclusionHigh dose Fluimucil can ameliorate the pulmonary function, relieve the symptom of short breath of patients with idiopathic pulmonary fibrosis.

Objective To investigate the relationship between relevant obstetrical factors and postnatal stress urinary incontinence. Methods Standard questionnaire on international urinary incontinence was applied, which was recommended by International Continence Society(ICS). Seven hundred and eighteen primiparas from the Obstetrical Department of Hebei United University Affiliated Hospital and Qinhuangdao Maternity and Child Care Hospital were randomly selected as our subject and fill out the survey. Six hundred and seventy-three primiparas were re-examined after delivery. Their postnatal situation of urinary incontinence after 42 days was recorded. Results ( 1 )Forty-one( 6. 09% ) primiparas of 673 primiparas were diagnosed with urinary incontinence after 42 days of their parturition. There were relationship between postnatal urinary incontinence with the mode of delivery,fetus body weight,progestational body weight and body mass index,and anal contraction movements(P < 0. 05).(2)The probability of the vaginal delivery group was 7. 76%(34 / 438), higher than that in primiparas with selective cesarean section group(2. 97%(7 / 235);χ2 = 6. 1;P < 0. 05).(3) The postnatal urinary incontinence in vaginal delivery group was closely related with fetus body weight, progestational body weight,body mass index( t = 4. 316,2. 093,2. 654;P < 0. 05). Conclusion (1)The postnatal urinary incontinence is more easily appeared in vaginal delivery.(2)Progestational primiparas with light body weight,fetus with less body weight,and anal contraction movements conducted in the postpartum period,can reduce the probability of postnatal urinary incontinence.(3)Mild urinary incontinence is more likely appeared during pregnancy and in the postpartum period. Therefore,more pelvic floor muscle exercise will have a better effect on preventing and curing postnatal urinary incontinence.

OBJECTIVE To observe bacterial distribution in urinary infection and trend of drug resistance in Shangdong Province.METHODS The patients with urinary infection monitored by Shandong Provincial Nosocomial Infection Surveillance System from Aug 2001 to Jul 2005 were analyzed and summarized.RESULTS Among 933 isolates,524 strains(56.16%) were Gram-negative bacilli,214(22.94%) were Gram-positive cocci and 195(20.90%) fungi.The most common pathogens were Escherichia coli(311),Candida albicans(74),and Enterococcus spp(62).Most of them were multidrug resistant.Most strains of Gram-negative bacilli were highly susceptible to imipenem,while most strains of Gram-positive cocci were highly susceptible to vancomycin.CONCLUSIONS Resistance detection of bacteria periodically has an important significance to clinical treatment with drugs.

Objective In recent years,as people have attached importance to the application of regional chemotherapy in the surgery of advanced gastric cancer,the research of fluorouracil sustained release implant,a regional chemotherapeutic drug,is on the increase.Fluorouracil sustained release implant is a new anti-carcinogen combined with fluorouracil,a conventional chemotherapeutic drug,through polymeric scaffolds and rete technology.It can be implanted into cancerous lesion area,as its slow-releasing potential and targeting property will give full play to the antitumor effect of fluorouracil,which can decrease the rate of local recurrence and metastasis of gastric cancer and prolong the life span of the patients.Based on the study of the researches on fluorouracil sustained release implant,a summary was made from different perspectives: basic medicine,pharmacy and clinical medicine.It may have reference value for clinical application and further study.

Objective To observe the role and effect of allitridi to cerebral vasospasm following expe rimental subarachnoid hemorrhage.Methods The models of Japanese rabbits with symptomatic cerebral vasospasm were established by blood injection of twice cisternal magna.Via one side subclavian artery,allitridi was continuously infused by a mini pump.Results After continuous intra arterial infusion allitridi 2 d,the diameter of basilar artery in the treatment group increased more obviously than that before treatment ( P 0.05).There was marked ischemic pathological change in the structure of basilar artery and brain in the control group,however there was no obvious change in the treatment group.Conclusion Allitridi can improve acute cerebral vasospasm;and the continuous intra arterial infusion allitridi has preventive effects in delayed cerebral vasospasm.