Objective To investigate the infection status and distribution characteristic of Helicobacter pylori (Hp)in different groups of population in Yangzhou.Methods 2 917 individuals from outpatients,hospitalized patients,and physical examination pop-ulation were recruited in the study.Serum samples from the subjects were rapidly detected using Helicobacter pylori urease antibody detection kits(Colloidal Gold method).The results were statistically analyzed.Results The totally Hp-detection rate of the 2 917 individuals was 32.9% (959/2 917).The Hp-detection rates of outpatients,hospitalized patients,and physical examination popula-tion were 33.1%,34.5% and 30.0% respectively.There were no significant difference in the positive rates between the male and female individuals(P >0.05).Overall,The Hp-positive rates increased with age.The minors(juveniles and children)had the lowest Hp-positive rate 16.0%(285/1 529);The Hp-positive rate of young adults was 34.0%(221/650),which was at the middle;elderly group had the highest Hp-positive rate 61.38%(453/758).59.8% of the patients diagnosed with gastrointestinal diseases were Hp positive.Among which,patients with peptic ulcer(74.4%)and gastric cancer(70.6%)have the highest detection rates.Some pa-tients with blood diseases,cardiovascular disease and some other diseases were also found with high Hp-detection rates.Conclusion The distribution of the Hp infection in population is positively correlated with age,but not with gender.Gastrointestinal diseases, especially peptic ulcers and gastric cancer,have high Hp infection rates,the relationships of Hp infections with some other diseases cannot be ignored and need to be further explored.

BACKGROUND: Schwann cells play an important role in regeneration of peripheral nerves and the extracellular matrix(ECM) is also important to growth of Schwann cells.OBJECTIVE: To explore the effects of laminin and type Ⅳ collagen on the adhesion and proliferation of Schwann cells.DESIGN: A controlled trial in groups with Schwann cell as subject.SETTING: Plastic Surgery Laboratory of the Fourth Military Medical University of Chinese PLA.MATERIALS: The trial was conducted in the Plastic Surgery Laboratory of Xijing Hospital Affiliated to the Fourth Military Medical University of Chinese PLA from January through April in 1995. The Schwann cells were extracted from newborn rabbits.METHODS: The culture plates were coated with laminin and type Ⅳ collagen. While in the control group, the antibody of laminin and collagen Ⅳ,collagen Ⅰ and polylysine were added respectively. The Schwann cells of the same concentration were cultured in plates of each group. The cells attachment rate in each group ,was determined after 2, 6, 24 hours respectively. After 72 hours 3H-TdR were incorporated into culture matrix fluid and double channel liquid scintillation counter was used for measurement of radiactivity count per minute.MAIN OUTCOME MEASURES: ① Attachment rate of Schwann cell. ②3H-TdR counting.RESULTS: The attachment rates in laminin and collagen Ⅳ groups (66%and 59% ) were higher than those of the type Ⅰ collagen and polylysine groups(45% and 43% ) after 2-hour culturing. Those in laminin antibody and collagen Ⅳ antibody groups were lower. The incorporation value were (10.0±2.7)×103, (1.3±0.3)×103, (10.4±2.4)×103, (1.4±0.5) ×103, (5.8±2.7)×103, (3.3±1.0)×103 per minute respectively in laminin, laminin antibody, collagen Ⅳ, collagen Ⅳ antibody, collagen Ⅰ and polylysine groups. The incorporation values in laminin and collagen Ⅳgroups were higher than controls.CONCLUSION: Laminin and type Ⅳ collagen promote the attachment and proliferation of Schwann cells in vitro. This study provides experimental basis for applying the action of Schwann cell to the nerve tissue engineering.

ObjectiveTo explore the effect of mental toughness level on positive and negative emotion and mental health in children with epilepsy.MethodsIn accordance with the International League against epilepsy and epilepsy syndrome in 1989 International Classification of young children with epilepsy,160 cases were sampled.Based on the Adolescent Resilience Scale ( HKRA ) score,according to the 27％ principles of delimitation,the patients were divided into high HKRA score group and low HKRA score group,43 cases in each group.Then positive emotions and negative affect scale (PANAS),mental health test (MHT) were applied to two groups.Results,Except family support dimension,the others HKRA dimension and total score were higher in female children with epilepsy than those in male patients,there was significant difference (P＜ 0.01 ).The positive affect score were higher in high HKRA score group than that of low HKRA group ; and negative emotion was significantly lower than that in low HKRA group (P＜ 0.01 ).Except physical symptom dimension,other dimensions and total score of HKRA in high HKRA score group were higher than those in low HKRA group,there was significant difference (P＜ 0.01 ).Children with epilepsy HKRA score was positive correlation with positive emotion score but negative correlation with negative affect scores (P ＜ 0.01 ) ; in addition to physical symptom,HKRA score and MHT score,and all the other dimensions were negatively related (P＜0.01 or 0.015).ConclusionThe mental toughness level are significantly related to children's epilepsy patients with positive and negative emotion and mental health status.A high level of mental toughness can increase positive emotional experience,and reduce the negative emotional experience of children with epilepsy.

ObjectiveTo study information transmission circumstances within the neural circuits related to the attentional controls of the mate depression patients.MethodsTwelve unipolar depression patients and twelve age- and education- matched healthy controls were involved in the Stroop word-color interference test to detect the variation of brain inhibitory function by Magnetoencephalography scanning.ResultsCompared with the healthy controls,the abnormally activities in depressed patients during in chronological order were separately as follow:decreased activation in the left postcentral gyrus ( t =3.10 ),the right superior frontal gyrus ( t =2.16 ),the left middle frontal gyrus (t=2.64),the left parietal lobe (t=2.89),the right middle temporal grus( t=1.92),the right superior temporal gyrus(t=2.84),the right insula(t=3.03),the right precuneus(t=2.98),the bilateral middle frontal gyros ( t =3.64,t =2.25 ),the right postcentral gyrus ( t =2.20),the right cingulate gyrus ( t =4.04 ),the bilateral precuneus ( t =2.79,t =1.87 ) ;increased activation in the left caudate body ( t =1.91 ),the left pulvinar ( t =2.56),the left middle temporal gyrus( t =1.89),the left precentral gyrus( t =2.02) (P ＜ 0.05,uncorrected).ConclusionThe abnormal activation in the brain regions related with attentional control processing is detected in the male recurrent unipolar recurrent major depression,performances of limbic system increased and.cortex declined more in the recurrent depressed.

ObjectiveTo evaluate the value of MicroPure software system in the display of calcification and diagnostic accuracy of breast carcinoma.MethodsSixty-three patients with 68 breast lesions were checked by conventional ultrasound,X-ray and MicroPure software technique.The calcification was described and the lesions were classified with BI-RADS.All cases were pathologically confirmed by biopsy or operation.Results ①Compared to the detection rate for calcification of X-ray,the display rate of conventional ultrasound was 80.6% ,and the display rate of MicroPure software technique was 100%.②The area under the curve of diagnosing benign and malignant of breast lesions was 0.916,0.857 and 0.970 respectively with ultrasound,X-ray and MicroPure software technique.③Three lesions was classified Ⅲ in BI-RADS with conventional ultrasound,and Ⅳ in BI-RADS with MicroPure software.The pathologic results were malignant.ConclusionsMicroPure software system obviously raised the ultrasonic display rate of calcification,meanwhile,it raised the diagnostic accurate rate of breast cancer.It especially raised the tendency rate for the patients who were classified between Ⅲ and Ⅳ in BI-RADS.

Objective To explore the clinical application of FP-PCR method to detect MP-DNA.Methods Five hundred and sixty-three children suspected of MP infection were enrolled in experimental group. FP-PCR was adopted to detect MP-DNA. MP-DNA was re-detected later in 60 children. At the same time,MP-Ab (MP antibody) was detected by means of particle agglutination. MP-Ab was re-detected one or two weeks later. Also 20 healthy children were selected as the control group. Results The positive rate of MP-DNA and MP-Ab were 34. 99% and 35.52% respectively,which showed no significant difference (x2 =0. 31, P > 0. 05). The coincidence of the two methods was 97. 69%. But the positive rate of MP-DNA was significantly higher than that of MP-Ab in the early stage(30. 48% vs 10. 16%) (x2 = 74. 46, P < 0. 05).The sensitivity and specificity of FP-PCR were 96. 00% and 98.62% respectively. The result of reviewed MP-DNA was consistent with the clinical diagnosis. Conclusion FP-PCR method is very sensitive, convenient and stable. It is fit for the clinical application ,especially the diagnosis of early MP infection. It helps to identify those who had been infected with MP before.

ObjectiveTo investigate the variation of executive function of fronto-cingulate cortex in spatio-temporal sequence in recurrent depressed patients.MethodsEighteen recurrent depressed patients and twenty-one age-,gender- and education- matched healthy controls were involved in the Stroop word-color interference test to detect the executive function of anterior cingulate and prefrontal regions by magnetoencephaloraphy scanning.Results 1.Compared to healthy controls,the increased activation of brain regions in depressed patients during different time periods were separately shown as follows( t represents activation strength):( 1 ) 100 ～ 200 ms:the right anterior cingulate gyrus ( t =1.81,T value means activation strength),the bilateral medial frontal gyrus ( t =1.91,t =1.86),the left superior frontal gyms( t =1.79 ),the right middle frontal gyrus( t =1.82 ),the right inferior frontal gyrus ( t =1.82 ).( 2 ) 200 ～ 300 ms:the left anterior cingulate gyrus ( t =1.82 ).( 3 ) 250 ～ 350 ms:the left anterior cingulate gyrus ( t =2.67 ),the left medial frontal gyrus ( t =2.43 ),the left inferior frontal gyrus ( t=1.91 ).(4)300 ～400ms:the left anterior frontal gyrus(t=2.26),the bilateral inferior gyrus( t=1.97,t=2.66),the left medial frontal gyrus(t=2.35).(5)350 ～450ms:the left anterior cingulate gyrus(t=2.02),the left medial frontal gyrus ( t =2.00),the bilateral inferior frontal gyrus ( t =1.74,t =1.78 ).( 6 ) 400 ～ 500 ms:the left anterior cingulate gyrus( t=2.01 ),the left medial frontal gyrus( t =1.97).2.Compared to healthy controls,patients showed decreased activation in the left middle frontal gyms ( t =1.79) and the left superior gyrus(t =1.76) in 200 ～ 300 ms.ConclusionExecutive dysfunction is reflected in depressed patients with the activation inefficiency in anterior cingulate and prefrontal regions in spatio-temporal sequence.

AIM: To elucidate the mechanism of fibrin in cholesterol gallstone disease. METHODS: Hemostatic indices were systemically examined in the gallbladder bile obtained from 26 patients with gallstones and 17 without gallstones. RESULTS: Significant increases were found in tissue factor (TF) concentration( P

AIM: To elucidate the relationship of inflammation, coagulation and cholesterol gallstone formation. METHODS: Hamsters were divided into four groups: control group (feeding normal diet), lithogenic diet (LD) 2 weeks group, LD 6 weeks group and LD+aspirin 6 weeks group. Gallstone incidence, antithrombin antigen (AT-Ⅲ:Ag), antithrombin activity (AT-Ⅲ:Ac), thrombin (F-Ⅱa:Ac), plasminogen activator inhibitor activity (PAI:Ac), plasmin activity (Plm:Ac), D-dimer:Ag and C-reactive protein (CRP) in gallbladder bile were observed as read-out parameters. RESULTS: The incidence of gallstones in control group, LD 2 weeks group, LD 6 weeks group and LD+aspirin group were 0%, 20%, 73% and 25%, respectively. AT-Ⅲ:Ag, F-Ⅱa:Ac, D-dimer and CRP in LD 2 weeks group and LD 6 weeks group were significantly higher than those in the control group (P

BACKGROUND:Our preliminary study found that the monocusp valves made of ultramicropore expanded
polytetrafluoroethylene (ePTFE) revealed no significant thrombus, calcification, or degradation 20 weeks after implanted into the descending aorta and the left pulmonary artery in sheep, which verified the good property of ePTFE. However, the surface of ePTFE in the left pulmonary artery was covered with obvious neointima.
OBJECTIVE: To assess the biocompatibility of phosphorylcholine-coated ePTFE.
METHODS:ePTFE surface was modified by phosphorylcholine derivative. Then the changes of surface shape, tensile stress at yield and elasticity modulus, water contact angle, and protein absorption capacity of ePTFE after surface modification were observed. (1) Hemolytic test: the leaching solution of phosphorylcholine-coated ePTFE, leaching solution of uncoated ePTFE, normal saline, and distiled water were added to the diluted human blood, respectively. (2) Platelet count test: the phosphorylcholine-coated ePTFE, uncoated ePTFE, high density
polyethylene, and Zymosan A were added to the whole blood samples from healthy volunteers, respectively.
(3) Platelet activation test: the phosphorylcholine-coated ePTFE, uncoated ePTFE, γ-Globulins, and Zymosan A
were added to the whole blood samples from healthy volunteers, respectively.
RESULTS AND CONCLUSION: The mean micropore diameter of ePTFE was significantly decreased after
phosphorylcholine coating (P < 0.001). The hydrophilicity and the ability of suppressing protein adsorption were
significantly strengthened after phosphorylcholine coating (P < 0.001). Phosphorylcholine coating did not influence
ePTFE in biomechanical properties and hemolytic test. The platelet count test and platelet activation test demonstrated that phosphorylcholine coating significantly improved anti-thrombus function of ePTFE. So, phosphorylcholine coating can enhance anti-thrombus function, suppress protein adsorption, and improve biocompatibility of ePTFE.