Objective To observe and analyze the effect of neoadjuvant chemotherapy on locally advanced non-small cell lung cancer tumor markers and lymphocyte subsets of different pathological type .Methods A total number of 40 NSCLC patients which received neoadjuvant chemotherapy and 20 normal people were selected in our study .To compare the differences of CEA ,CA-125 , CYFRA21-1 and lymphocyte subset in NSCLC pantients with adenocarcinoma or squamous cell carcinoma and the the differences of CEA ,CA-125 ,CYFRA21-1 and lymphocyte subset in patients with different clinical effect .Results (1) The CEA ,CA-125 ,CY-FRA21-1 in pantients with adenocarcinoma or squamous cell carcinoma were both higher than normal people .The CEA level in pa-tients with adenocarcinoma was higher than squamous cell carcinoma ,while the CA-125 and CYFRA21-1 in squamous cell carcino-ma were higher than adenocarcinoma .The CD3+ ,CD4+ ,CD8+ ,CD4+ /CD8+ in pantients with adenocarcinoma or squamous cell carcinoma both had obvious differences with normal people .But there were no obvious differences between patients with adenocarci-noma and squamous cell carcinoma .(2)There were 18 cases of CR+ PR while 22 cases of SD+ PD .The CA-125 and CYFRA21-1 were decreased in patients of squamous cell carcinoma with clinical effective while the CEA and CYFRA 21-1 were decreased in ade-nocarcinoma with clinical effective .The CD3+ ,CD4+ ,CD4+ /CD8+ increased and CD8+ decreased in patients of squamous cell car-cinoma or adenocarcinoma with clinical effective .Conclusion The tumor marker and lymphocyte subsets values had obvious differ-ences in adenocarcinoma and squamous cell carcinoma .Both tumor marker and lymphocyte subsets were useful in diagnosis .

Hepatocellular carcinoma(HCC),one of the most common malignant tumors,is the main cause of cancer death in China.Early diagnosis of the disease is of great importance.Serum tumor markers have been effective for detecting HCC for a long time.Among those markers,alpha fetoprotein(AFP)is the most widely used one in detecting patients with HCC,but it has limited utility for detecting HCC due to its limited sensitivity and specificity.Searching better markers for HCC has been a re- search focus in recent years.This review introduces many useful markers to supplement AFP for detecting HCC.

Acute Disease ; Cytogenetics ; Humans ; Leukemia ; genetics

Objective To study lentinan’s promotion on XELOX regimen’s curative effects on advanced gastric cancer. Method Cases with advanced gastric cancer were divided into observation group and control group according their therapy method. The curative effects, side effects, WBC, lymphocyte subsets, NK cells and quality of life were compared. Results The difference of disease control rates in two groups were not significant (P=0.091). The incidences of side effects in observation group were significantlly lower than in control group (P<0.05). The observation group’s WBC, Lym, CD 3+, CD 4+, CD 8+, NK cells and quality of life were significantly higher than in control group(P<0.05). Conclusion Lentinan could significantly promote gastric cancer patients’immunity and lessen side effects. It can promote XELOX regimen’s curative effects on gastric cancer and improve quality of life.

Objective To evaluate the effects of intraaortic balloon pump (IABP) support in the emergency interventional procedures on pump failure or cardiogenic shock complicating acute myocardial infarction (AMI). Methods Group A included 21 patients with IABP support during the emergent interventional procedures, and group B included 66 patients without IABP. The two groups were compared for clinical criteria and in-hospital events or complications.Results No significant differences were noted between the two groups witch regard to baseline age, gender, onset of chest pain, timing of intervention, location of AMI, the number of coronary artery diseases, individual infarct-related artery, and TIMI grade before intervention. The degree of pump failure was significantly worse in group A than in group B. In a follow-up period of in-hospital, mortality and revascularization in-hospital were lower in the patients with IABP than in the patients without IABP. Conclusion The combination of early IABP and successful emergency coronary intervention is associated with improved survival in patients with pump failure or cardiogenic shock complicating AMI.

Objective To determine the serum level of KL-6 in patients with polymyositis(PM)and dermatomyositis(DM),and to investigate the possible diagnostic value for interstitial lung diseases(ILD)in pa- tients with PM/DM.Methods Serum KL-6 concentrations were measured by ELISA in 53 adult PM/DM pa- tients,the control groups of 22 patients with infectious lung disease,and 50 healthy subjects.The association with clinical features and serum KL-6 levels was analyzed.Results The serum levels of KL-6 were(1543?761)、(429?106)、(336?196)and(289?105)U/ml in PM/DM patients with ILD and without ILD,patients with infectious lung disease and healthy controls,respectively.Serum KL-6 levels in the PM/DM patients with ILD were significantly higher compared with PM/DM patients without ILD,patients with infectious lung disease and healthy controls(all P＜0.01 ).However,no significant differences of serum KL-6 levels was found among PM/ DM patients without ILD,patients with infectious lung disease and healthy controls(P＞0.05).Significant cor- relations were found between the elevated levels of serum KL-6 and the presence of ILD in patients with PM/ DM(P＜0.01).The sensitivity and specificity of serum abnormal KL-6 levels for ILD in patients with PM/DM were 90.9% and 80.6%.Additionally,follow-up study showed the mean serum levels of KL-6 in six patients who died were significantly higher than that in other PM/DM patients with ILD(P＜0.01).Conclusion Serum KL-6 level is a reliable serum marker for ILD in idiopathic inflammatory myopathies,which may contribute to early differentiate ILD from lung infectious disease.Increased serum level of KL-6 may predict a poor out- come.

Hair follicle stem cells located bulge region of hair follicle,had characteristics of all adult stem cells,including slow-cycling,undifferentiated.They also had the abilities of self-renew and proliferation in vitro.CD34,K15,K19 and Nestin might be the makers of the hair follicle stem cells.They could be induced to differentiate to neurons,glial cells,keratinocytes,smooth muscle cells,melanocytes In vitro and neurons,melanocytes in vivo.There were many signals in regulating the hair follicle stem cells involved Wnt signal,BM Psignal and NFATc1 etc.

Animals ; Carcinoma, Hepatocellular ; blood supply ; pathology ; therapy ; Cell Line, Tumor ; Cells, Cultured ; Cytotoxicity, Immunologic ; immunology ; Female ; Humans ; Interleukin-12 ; pharmacology ; Interleukin-2 ; pharmacology ; Killer Cells, Natural ; cytology ; drug effects ; immunology ; Liver Neoplasms, Experimental ; blood supply ; pathology ; therapy ; Lymphocyte Activation ; drug effects ; immunology ; Male ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Microcirculation ; drug effects ; Random Allocation ; Xenograft Model Antitumor Assays

Animals ; Bone Marrow Cells ; Cord Blood Stem Cell Transplantation ; adverse effects ; Female ; Graft vs Host Disease ; Male ; Mesenchymal Stromal Cells ; Mice ; Mice, Inbred C57BL ; Pregnancy

Objective To study the effect of mechanical tension on collagen arrangement and illustrate the relationship between tissue architecture and tension properties. Methods Cell morphologies, orientation and collagen arrangement of fibroblasts cultured in three different types of collagen gels with variation of mechanical tension were observed by phase contrast photomicrographs, light microscopy and transmission electron microscopy. Expression and distribution of a-smooth muscle actin (α-SMA) were investigated by immunohistochemistry. Results Phase contrast photomicrographs, light microscopy and transmission electron microscopy showed high level of tension distributed anisotropically in the monolayer gels and the anchored collagen gels, with bipolar shape of the fibroblasts, obvious polarity, arrangement of exogenous collagen fibres parallel to the long axis of the fibroblasts, especially prominent in monolayer gels. Low level of tension distributed isotropically was observed in floating collagen gels, with stellate morphology and arrangement of exogenous collagen fibres in a reticular array. Immunofluorescence showed that fibroblasts expressed high level of α-SMA protein distributed along the long axis of fibroblasts in the monolayer gels and the anchored collagen gels, especially in former ones. In contrast, few expression of α-SMA protein was found in floating collagen gels. Cell morphologies and orientation, expression and distribution of α-SMA as well as collagen arrangement of fibroblasts in the monolayer gels and the anchored collagen gels were similar to those in granulation tissue, whereas floating collagen gels resembled normal dermis or remodelled tissues. Conclusions Tissue architecture or morphology of the dermis are corresponding to tension proporties. Different tissue architectures are closely correlated with particular tension proporties.