Objective To explore the clinical value of IgE and IL-5 in diagnosis of mycoplasma pneumoniae children with bronchial asthma.Methods 40 mycoplasma pneumoniae infection children complicated with bronchial asthma were chosen as the observation group,the other 40 mycoplasma pneumoniae infection children without bronchial asthma were selected as the control group.All children were admitted to hospital,the next morning fasting blood was obtained to detect IgE and IL-5 levels.Results The serum total IgE level of observation group was (335.74 ±38.84) IU/ml,the level of IL-5 was (311.86 ± 35.28) ng/L,which were significantly higher than control group (P ＜0.05).Binary Logistic regression analysis showed that,serum IgE level and IL-5 level had statistically significant difference (P ＜ 0.05).Conclusion Mycoplasma pneumoniae infection in children with bronchial asthma,the IgE and IL-5 levels increased more obviously,and the levels of serum IgE and IL-5 were important risk factors of onset of children with Mycoplasma pneumoniae infection combined bronchial asthma.

The paper presents a summary of theories on the impact of health on economy and makes an empirical analysis of the impact from three aspects,viz.health and economic development,health and personal income,and health and economic growth.In light of economic development in the past and studies in recent years of the relationship between health human capital,personal income and economic development in micro and macro economics,the paper argues that health human capital has contributed to the rise of personal income by improving individual labor market performance and has directly promoted economic growth by constituting a form of investment.

Objective To compare the differences of blood glucose detected by four methods with different instruments and specimen types at early stage in severely burned rats.Methods 24 Sprague-Dawley (SD) rats were randomly divided into two groups:control group 1 (Sham scald group,n=8) and scald injury group 1 (n=16).Blood samples of scald injury group 1 were collected at 12,and 24 hours after scald (n=8,each time).Another 20 SD rats were randomly divided into two groups:control group 2 (Sham scald group,n=10) and scald injury group 2 (n=10).Blood samples of scald injury group 2 were collected at 12 hours after scald.The rats in scald injury group 1 and 2 were placed into scalding water (95.0±0.5)℃ for 15 seconds to model third-degree burn with 30％ total burn surface area (TBSA).The rats in scald injury group 1 were given intraperitoneal injection with normal saline(40 ml/kg) immediately,while those in scald injury group 2 were given intraperitoneal injection with normal saline (40 ml/kg) 6 hours after scald.The rats in Sham scald group 1 and 2 were placed into warm water 37℃ for 15 seconds,and did not received injection.Portable glucometer/caudal artery (vein) blood,portable glucometer/abdominal aorta blood,spectrophotometer/femoral venous plasma,and spectrophotometer/abdominal aorta plasma were used to detect blood glucose.Results ①Compared with Sham scald group 1,the levels of blood glucose detected by portable glucometer/caudal artery (vein) blood and spectrophotometer/femoral venous plasma in scald injury group1 at 12,24 hours after scald were significantly increased(P＜0.05).Compared with Sham scald group 2,the levels of blood glucose detected by portable glucometer/abdominal aorta blood and spectrophotometer/abdominal aorta plasma in scald injury group 2 at 12 hours after scald were significantly increased(P＜0.05).②The comparison of portable glucometer/caudal artery (vein) blood and spectrophotometer/femoral venous plasma in Sham scald group 1,portable glucometer/abdominal aorta blood and spectrophotometer/abdominal aorta plasma in Sham scald group 2 had no statistical significance (P＞0.05).The levels of blood glucose detected by portable glucometer/caudal artery (vein) blood were significantly lower than those detected by spectrophotometer/femoral venous plasma (P＜0.05) in scald injury group 1.The comparison of blood glucose detected by portable glucometer/abdominal aorta blood and spectrophotometer/abdominal aorta plasma had no statistical significance in scald injury group 2 (P＞0.05).Conclusion Four kinds of methods used in this study shows that the levels of blood glucose were significantly increased at early stage in severely burned rats,and the portable glucometer/abdominal aorta blood is a relatively simple and fast method to detect blood glucose.

ObjectiveTo investigate the effects of intramuscular Botulinum toxin type A (BTX-A) injection on spasticity of lower limb, walking and activities of daily living (ADL) after stroke. Methods13 patients with spasticity on lower limb were injected intramuscularly with BTX-A, and assessed with Composite Spasticity Scale (CSS), step length and gait velocity, and Barthel index (BI) before and 1, 4, 8 and 12 weeks after treatment. ResultsThe scores of CSS and BI significantly improved after treatment (P<0.05). There was some improvement in step length and gait velocity, but not significant (P>0.05). All the improvement appeared 1 week after injection and lasted to the 12th week. ConclusionLocal intramuscular BTX-A injection can reduce the spasticity on lower limb and improve ADL.

Objective　To explore the clinical superiority of two kinds of chopping nucleus methods in phacoemulsification——etch and turn technique, stop and chop technique.Methods　Three hundred and sixty-five eyes were performed with phacoemulsification, among which 85 eyes were performed with turn technique and 280 eyes were performed with chop technique.The results of the two kinds of chopping nucleus were analyzed.Results　(1)Time of chopping nucleus:time of turn technique averaged 81.26 seconds while chop technique averaged 40.71 seconds; (2)Visual acuity in the first week after operation:turn technique:vision of 76 of 85 eyes were 0.5 of better, 7 eyes were 0.2～0.5(8.2%) and 2 eyes were 0.2 or worse; chop technique: vision of 253 of 280 eyes were 0.5 or better (90.4%), 21 eyes were 0.2～0.5(7.5%) and 6 eyes were 0.2 or worse (2.1%);(3)Reaction of corneal endothelial cells after the operation: central endothelium rugosity appeared in 4 eyes (4.7%) with turn techinque and in 7 eyes (2.5%) with chop technique. Conclusion　The operation of the former is less difficult and the chopping nucleus time is longer. The time of the operation on the latter is shorter and it is appriate for hard nucleus and should be applied and spreaded. There are no marked differences as for the vision after the operation and the microcorneal injury between them.

Objective To investigate the association of platelet-derived growth factor(PDGF)-B gene single nucleotide polymorphism(SNP) and plasma PDGF-BB level with in-stent restenosis(ISR) in elderly patients.Methods 157 patients who had undergone coronary artery stenting for more than half year were divided into ISR group(n=74) and NISR group(non-ISR,n=83) according to the angiographic diagnosis of in-stent restenosis (ISR).Plasma level of PDGF-BB was measured by enzyme-linked immunosorbent assay(ELISA).DNA was isolated from leukocytes.Two SNPs of the PDGF-B gene(rs1800818 and rs1800817) were determined by Taqman Quantitative Real-Time PCR with TaqMan-MGB probe.Results There were no significant differences in genotype frequency of rs1800818 AA,AG,GG between ISR group and NISR group(x2 =4.48,P＞0.05).The frequency of rs1800818A allele was much higher in ISR group than in NISR group(x2 =5.33,P＜0.05).There were no significant differences in genotype frequency of SNP rs1800817 AA and AC(x2 =0.06,P＞ 0.05) and allele frequency of SNP rs1800817 A and C(x2 =0.06,P＞0.05) between ISR group and NISR group,while genotype CC was not found.The plasma level of PDGF-BB was higher in ISR group than in NISR group [(6.53±3.65) ng/L vs.(5.07±2.45) ng/L,t=2.92,P＜0.01].Plasma level of PDGF-BB in patients with rs1800818 AA genotype was significantly higher in ISR group than in NISR group [(9.94 ± 4.60) ng/L vs.(5.90 ± 2.98) ng/L,t =2.69,P＜0.05].Multivariable logistic regression analysis showed that plasma PDGF-BB level was the risk factor for ISR (OR =1.187,95.0％ CI:1.054 1.337,P＜0.01).Conclusions High plasma PDGF-BB level is the risk factor for ISR,but PDGF-B gene SNPs rs1800818 and rs1800817 are not associated with the occurrence of ISR.

Objective To investigate the application effect of montelukast in children with mild asthma.Methods 120 patients with mild asthma were randomly divided into the treatment group and the control group,the control group was given conventional therapy,the treatment group was treated with montelukast on the basis of the control group.Two groups were treated for 4 weeks as a course.Results The total effective rate of treatment group was 91.7％,which was significantly higher than 73.3％ in the control group (x2 =3.89,P ＜0.05).The daytime and nighttime asthma score in the treatment group were (0.13 ± 0.05)points,(0.12 ± 0.04)points,which were significantly lower than (1.13 ± 0.21) points,(0.43 ± 0.23) points in the control group.The symptom-free days was (17.43 ± 2.87) d,which was significantly longer than (9.34 ± 1.57) d in the control group(t =2.32,1.97,5.75,all P ＜ 0.05).IL-6,TNF-α,IgE in the treatment group after the treatment were (140.5 ± 6.4) ng/L,(40.1 ± 4.9) IU/ml,(105.6 ± 8.8) IU/ml,which were significantly lower than (189.3 ± 9.7) ng/L,(78.6 ± 7.5) 1U/ml,(155.4 ±10.5) IU/ml in the control group (t =11.97,8.75,13.56,all P ＜ 0.05).The incidence rate of adverse reaction in the treatment group was 13.3％ and 10％ in the control group,there was no significant ditterence(x2 =1.32,P ＞0.05).Conclusion Montelukast can significantly improve the inflammatory state in children with mild asthma,relieve clinical symptoms,improve the therapeutic effect,and has less adverse reactions.

In many medical instrumentation systems, there exists a long distance between manipulation platform and host unit. Wireless data transferring between the platform and the host could be a feasible way to set parameters. This paper introduces wireless transmitter and receiver circuit based on encoder chip PT2262 and decoder chip PT22720, and presents some typical applications in medical instrument development.

Objective To investigate the role of antisense RNA of plasminogen activator inhibitor 1 (PAI 1) in regulating the expression of PAI 1 and vascular endothelial growth factor (VEGF) in aorta endothelial cells (EC) cultured in vitro. Methods The second extron of PAI 1 was amplified by polymerase chain reaction(PCR) and the product was inserted into eukaryotic cell expression vector pcDNA3.1(-) to construct PAI 1 antisence RNA recombinant plasmid. The recombinant plasmid was transfected into EC and the PAI 1 expression was detected by immunohistochemistry, Westernblot and ELISA. The effects of PAI 1 variation on VEGF were examined by immunofluorescence method. Results PAI 1 antigen was the lowest (0 017 ng/ml) in cells and the immunofluorescence representing the expression of VEGF in the cytoplasm showed the weakest at the third day after transfection. At the fifth day, PAI 1 antigen increased to 0 093 ng/ml with VEGF expression increased correspondingly. At the seventh day, PAI 1 antigen(0 143 ng/ml) and VEGF increased closed to normal level. Conclusions PAI 1 antisense RNA blocked the translation of PAI 1 proteins effectively and inhibited the expression of VEGF in aorta endothelial cells.

Objective To reveal the fibrillar network in vitreous and the effect of plasmin on this network. Methods 20 vitreous gels of freshly slaughtered pigs were divided into 2 groups, the gels in first group were digested by 3 U plasmin (3 U/ml) at 37℃ for 24 hours respectively, the second group received the same PBS as control. After digestion, gels were fixed in neutral buffered formalin solution. Samples from vitreous base, cortex and the central region were observed by the technique of freeze etching electron microscopy. Results In vitreous collagen fibril network was in a three-dimensional array, collagen fibril density showed marked differences, central vitreous had the sparse fibril density, the cortex denser and the basal vitreous densest. After digestion by plasmin, the collagen fibrillar network was destructed. Conclusion Collagen fibrils in vitreous present spatial arrangement regularly, plasmin can lead to destruction of the fibrillar network.