0.05). Conclusions Autop-hagy/lysosomal pathway was activated immediately after recurrent seizures as indicated by the elevated expression of Beclin1 in cerebral cortex. 3-MA was involved in the regulation of autophagy/lysosomal pathway by down-regulating the expression of Beclin1.

ted in a dose-dependent manner (P<0.01). Conclusion Quercetin can significantly inhibit the proliferation of HeLa cells, which may be induced apoptosis of cervical cancer cells via the Ca2+-dependent mitochondrial apoptosis pathway.

One hundred and ninety-three patients undergoing transvaginal hysterectomy for cicatrix uterus (study group), 200 patients receiving transabdominal hysterectomy for cicatrix uterus (control group Ⅰ), and 200 patients having transvaginal hysterectomy for non-prolapsed uterus (control group Ⅱ) were retrospectively analyzed. In comparison with the control group Ⅰ, the study group showed a significantly reduced operation time and the average volume of blood loss (P < 0.01). Moreover, patients in the study group had shorter postoperative recovery time (P < 0.01) . The incidence of postoperative fever was decreased in the study group. There was no significant difference in complications of the vaginal wound between the three groups (P 0.05). This investigation demonstrates that transvaginal hysterectomy might be feasible and safe in removing cicatrix non-prolapsed uterus.

Objective To evaluate the effect of methylprednisolone on hepatic ischemia-reperfusion (I/R) injury in the patients undergoing hepatolobectomy.Methods Sixty ASA physical status Ⅱ or Ⅲ patients,aged 30-64 yr,weighing 45-75 kg,scheduled for elective hepatolobectomy,were randomized to control group or methylprednisolone group (n =30 each).After induction of anesthesia,methylprednisolone 500 mg (in 100 ml of normal saline) was infused intravenously at 5 ml/min before skin incision in group M.Anesthesia was induced with propofol,fentanyl and cisatracurium.The patients were endotracheally intubated and mechanically ventilated.PETCO2 was maintained at 35-45 mmHg.Anesthesia was maintained with 1％-3％ sevoflurane inhalation,remifentanil infusion,and intermittent iv boluses of fentanyl and cisatracurium.MAP was maintained at 70-100 mmHg and HR at 50-90 bpm.At 10 min before induction of anesthesia,and on postoperative day 1,3 and 5,venous blood samples were collected for determination of the plasma levels of alanine aminotransferase (ALT),aspartate amminotransferase (AST),total bilirubin (TBIL),tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6).Results Compared with group C,the plasma levels of ALT,AST and TBIL were significantly decreased on postoperative day l and 3,and the plasma concentrations of TNF-α and IL-6 were decreased on postoperative day 1,3 and 5 in group M.Conclusion Methylprednisolone can reduce hepatic I/R injury in the patients undergoing hepatolobectomy and inhibition of systemic inflammatory responses is involved in the mechanism.

Linolenic acid containing selenium was synthesized and brought into emul- sion(82). Its aatitumor effects were tested in vitro and in vivo. As a result, when 82 was injected peritoneally into mice(200mg?kg-1?d-1), its inhibitory rate on mouse S180 was 42.6%(P

AIM: To establish the method for quality control of Guli Granules(Herba Epimedii,Radix Puerariae Lobatae,Rhizoma Curcumae Longae,Radix Achyranthis Bidentatae,Fructus Psoraleae,etc.). METHODS: Radix Puerariae,Rhizoma Curcumae Longae,Radix Achyranthis Bidentatae and Fructus Psoraleae in this medicine were identified by TLC.The icarrin content in this medicine was determined by HPLC.The acetonitril-water(28∶72) solution was used as the mobile phase,the column was C_(18),the flow rate was 1.0 mL?min~(-1).The detection wavelength was at 270 nm. RESULTS: These herbs could be identified by TLC.For Icarrin,the linear range was from 17 to 85 ?g?mL~(-1),and the average recovery was 98.32% with RSD of 1.67%. CONCLUSION: The method is simple,reliable,accurate and can be applied to the quality control of the preparation.

Objective To evaluate the value of volume rendering technique of MSCT in the diagnosis of pediatric pectus carinatum. Methods MSCT data of 36 children with pectus carinatum were collected.Volume rendering and multiplanar reformation recon-struction techniques were adopted at a postprocessing station.Results There were 35 cases of chondrogladiolar variant and 1 case of chondromanubrial variant,27 cases were symmetric and other 9 cases were asymmetric.VR technique clearly demonstrated the abnormal changes of sternum,costae and costal cartilages.Conclusion VR technique of MSCT can fully display the three-dimension morpho-logical changes of pediatric pectus carinatum,which can supply accurate information for minimally invasive treatment procedure.

Objective To discuss the blockage reasons and countermeasures of temporary catheter in central venous including femoral vein and internal jugular vein for hemodialysis patients.Methods The reasons of blockage of temporary catheter in central venous among 60 hemodialysis patients from January 2014 to September 2015 in Hemodialysis Room of the First People's Hospital of Longquanyi district of Chengdu were reviewed.There were 32 cases in femoral vein and 28 cases in the right internal jugular vein.The incidence of blockage of the two temporary catheter in the first two weeks,the incidence of blockage caused by mechanical factors and blood clots or fibrous sheath and the incidence of blockage which needed to change the catheter in the two groups patients within the first two months were analyzed.Results The first rate of defective function in the first two weeks of femoral vein group was 53.13％(17/32),and 7.14％(2/28) of the right internal jugular vein group,there were significant difference between the two groups (x2=13.061,P＜0.001).The incidence of catheter blockage due to mechanical factors of femoral vein group was 96.88％ (31/32),and 53.57％ (15/28) of the right internal jugular vein,there were significant difference between the two groups (x2 =15.654,P＜0.001).The incidence of blockage caused by blood clots or fibrous sheath and the incidence of blockage which needed to change the catheter in the first two months were no significant differences between the two groups(P=0.061,0.096).Conclusion The cases of temporary catheter in femoral vein have blockage earlier,and they have a higher risk of catheter jam than the cases of temporary catheter in right internal jugular vein.

Objective To study the pattern of cerebral gray matter and white matter volume changes among smokers with differ-ent level of nicotine dependence (addition)using voxel-based morphometry (VBM).Methods The current case-control study recrui-ted 53 healthy male smokers and 53 healthy non-smokers from outpatients of our hospital during January 2013 to May 2014.Personal information (including for example age,sex and addition dependence of subjects)was collected using a questionnaire.3D-T1 images of whole brain structure were collected and were analyzed using DARTEL toolbox of SPM8.Smokers were divided into mild to mod-erate nicotine dependence group (n=23)and severe nicotine dependence group (n=30)based on Fagerstr?m Test for Nicotine De-pendence (FTND)score.Independent sample t-test analyses were performed to compare the volumes of gray matter and white mat-ter between smokers with different levels of nicotine dependence and non-smokers.Results Compared with non-smokers,gray and white matter volumes of smokers were smaller in multiple brain areas,mainly in the middle occipital gyrus,posterior cingulate,cer-ebellum anterior lobe,precuneus,caudate body and insula,which however,had larger number and scope of focal areas with gray and white matter atrophy in the mild to moderate nicotine dependence group than that in the severe nicotine dependence group.Conclusion Smokers with mild to moderate nicotine dependence have more pronounced gray and white matter atrophy than that smokers with severe nicotine dependence have.

Objective To explore the application and effect of three-dimensional animation teaching software combined with case based learning ( CBL ) in inguinal hernia clinical teaching . Methods Totally 30 eight-year program students of Grade 2008 of Peking University People's Hos-pital were randomly divided into the teaching software combined with the CBL group(n=15) and tradi-tional teaching group(n=15). Three-dimensional animation teaching software was used to simulate the whole process of inguinal hernia anatomy and physiology, pathogenesis, clinical manifestations, diag-nosis and treatment in teaching software combined with CBL group. CBL teaching was conducted by setting problems through typical case and case discussion under the guidance of the teacher . The teacher-centered teaching, teaching rounds and watching operation were used in the traditional teaching group . Theory examination and physical examination were given at the end of the study and achieve-ment differences were compared between 2 groups. Questionnaire was used to investigate students' evaluation of the teaching method in teaching software combined with the CBL group and data were statistically described using t test. Results Theoretical exam score of the teaching software combined with the CBL group was (94.80±1.54), superior to the traditional teaching group(86.10±1.86), and the difference was statistically significant(t=8.242, P=0.000). Clinical skill test score of the teaching software combined with the CBL group was (91.10±1.01), superior to the traditional teaching group (85.90±2.17), and the difference was statistically significant(t=8.531, P=0.000). The survey result showed that more than 80.00% students thought that this teaching method could arouse students' learning enthusiasm, promote their mastery and application of knowledge, strengthen their self-study ability, cultivating their clinical thinking and improving their learning efficiency. Conclusion The teaching method of three dimensional animation teaching software combined with CBL , as a new teaching mode, helps to improve the quality of teaching.