Bleeding Time ; Child, Preschool ; Female ; Hemorrhage ; diagnosis ; Humans ; von Willebrand Diseases ; diagnosis

Biomedical Research ; Humans ; Tai Ji

Objective To investigate the inhibitory effects and mechanism of ginsenoside Rg3 on vasculogenic mimicry of human breast cancer MCF-7 cell line. Methods The MCF-7 cells at logarithmic growth phase were obtained, and were cultured with different concentrations (0, 20, 50, 100, 150 and 300 mg/L) of ginsenoside Rg3. Cells cultured without Rg3 were served as controls. The IC50 were determined by CCK8 assay and anti-angiogenic effects were performed for testing the potential of tube-like structure (TLSs) formation. The expression levels of VEGF-A, MMP 9 and HIF-1αwere detected by Western blotting and real-time PCR. The secreted contents of VEGF-A and MMP9 were detected by enzyme linked immunosorbent assay (ELISA). Results The ginsenoside Rg3 suppressed the proliferation of MCF-7 cells in a dose-dependent manner, in which IC50 was (115.34±8.50) mg/L. The formation numbers of TLSs in MCF-7 cells were significantly inhibited by Rg3 in concentration dependent manner in 50 mg/L, 100 mg/L and 150 mg/L for (19.0 ± 1.0), (15.0 ± 1.5), and (10.0±1.7) vs. controls (22.0±1.8, F=150.805, P<0.05). The mRNA and protein expression levels of VEGF-A, MMP9 and HIF-1αprotein were inhibited by 50 mg/L,100 mg/L and 150 mg/L Rg3 vs. controls (P<0.05). Meanwhile, the contents of VEGF-A in MCF-7 cell supernatant was down-regulated by 50 mg/L,100 mg/L and 150 mg/L Rg3 vs. controls (P<0.05). The contents of MMP-9 in MCF-7 cell supernatant was down-regulated by 100 mg/L and 150 mg/L Rg3 vs. controls (P<0.05). There was no significant difference in MMP-9 expression between 50 mg/L group and control group. Conclusion The ginsenoside Rg3 is able to inhibit the vasculogenic mimicry of MCF-7 cells, which may be related with the down-
regulation of VEGF-A, MMP9 and HIF-1α.

Objective To observe the effect of arthroscopic meniscectomy to tr eat discoid lateral meniscus tear. Methods From July 1999 to June 2003, arthrosc opic meniscectomy was conducted for 49 menisci of 45 patients with discoid later al meniscus tear. 41 patients had unilateral injury, and 4 bilateral. 32 knees w ere complete injury and 17 incomplete. The discoid lateral meniscus tear account ed for nearly one third of the patients with meniscus tear who received arthrosc opic meniscectomy at the same time. Arthroscopic partial meniscectomy was done f or 44 knees, total meniscectomy for 5 knees and meniscus suture for 3 knees. Aft er operation the rehabilitation training programs, including straight-leg-rais ing exercise and range-of-motion exercise, were carried out. Results The opera tions for the 45 cases were successful and there was no complication. 40 patient s were followed up. Before operation, the mean Lysholm -Ⅱscore was 55 points ( 40 to 71 points). After a mean follow-up period of 39 months, the mean Lysholm -Ⅱscore was 88 points (60 to 100 points). The excellent and good results were obtained in 85.3%of the patients. Conclusions Arthroscopic meniscectomy should be a preferred method for discoid lateral menisci tear, due to its minimal invas ion, possibility of early mobilization, a lower complication rate, and preservat ion of more meniscus structure and function. If the operation is combined with s tandard rehabilitation training, the effects can be enhanced.

Mitogen-activated protein kinases (MAPKs) are important kinases involved in the intracellular signaling transduction in mammalian. There are three major MAPK pathways: ERK/MAPK,JNK/SAPK and P38 MAPK. Current studies indicate that all of them play significant roles in the processing of pain especially in the development and maintenance of pathological pain. This review will focus on in the study of MAPK in pain modulation. The further insights into its mechanisms may highlight a nice prospect for the therapies of painful diseases.

Objective To investigate the effect of milk basic protein (MBP) on bone metabolism in normal and ovariectomized (Ovx) rats. Method Forty-eight female Sprague-Dawley rats were ovariectomized and another 12 rats received sham operation (Sham). After 10 d recovery period, the Ovx rats were randomly divided into 4 groups: control, low-dose, medium-dose, and high-dose MBP group. Another 44 normal female rats without ovariectomy were also divided into 4 groups as above. The MBP dosages for each group were respectively 0, 10, 20, 30 mg/kg bw. All rats were i.g. administered for 90 d. Bone mineral density (BMD) of the femur (at proximal end, middle of diaphysis, and distal end) was measured by dual-energy X-ray absorptiometry in vivo. The amounts of calcium, magnesium and phosphorus were analyzed by ICP-AES. Results BMD at distal end of femur was significantly higher in normal low-dose group than in normal control group while no significant effect was observed in Ovx MBP groups. As for the amounts of calcium, magnesium and phosphorus, there were no significant differences among normal experimental groups and also among Ovx experimental groups. However, some variations in the level of those minerals were observed. Conclusion MBP at 10 mg/kg bw significantly elevated BMD at femoral distal end in normal rats, while no similar effect was observed in Ovx rats. Besides its influence on bone minerals, there may be another mechanism involved in its effect on bone metabolism.

Objective To investigate the relationship between metabolic syndrome(MS)and nonalcoholic fatty liver disease (NAFLD)in non-obese and non-diabetic hypertensive patients.Method 84 non-obese and non-diabetic hyperlensive patients were divided into 42 hypertensive patients with NAFLD and 42 hypertensive patients without NAFLD by abdominal ultrasonography examination.Body mass index(BMI),blood pressure,fast and OGGT 2 hour plasma glucose,fast and OGGT 2 hour plasma insulin,plasma lipids and aminotransferase were measured in the two groups.Multivariate logistic regression analysis was used to identify the variables independently associated with MS.Result BMI,triglycerides,fast insulin,2 hour insulin,aminotransferase,HOMA-IB and MS prevalence rate in the group with NAFLD were significantly higher than these in the group without NAFLD.Multivariate logistic regression analysis(forward LR)showed NAFLD:were independently associated with MS.Conclusion MS play a role not only in occurrence of NAFLD but also in liver injury.

Objective:To detect mRNA expression of IL-6 and IL-8 in allergen-induced late-phase cutaneous reactions in schneiderian membrane subjects. Methods:Cryostat sections from rhinitis biopsies from 24 h allergen-induced late-phase cutaneous reactions (LPR) in 10human atopic subjects were hybridization with 35S-labeled RNA probes for IL-6 and IL-8.Results:mRNA was detected for IL-6 (9/10) and IL8 (10/10).Compared with the control, there were significant increases in the numbers of ce11 expreasing mRNA expression for IL-6 and IL-8(P＜0.05, P＜0.01). Conclusion: The augmentation of mRNA expression of IL-6 and IL-8 maybe regarded as the mark of rhinits in IL PR.

Objective To approach the feasibility of the development and routinely carrying out of gynecological analgesia. Methods A total of 286 pregnant women, 58 were primiparae with full-term fetus and 228 were for mid-term delivery or induced abortion, were enrolled in present study. The combined lumbar-epidural analgesia (CLEA) and the patient-controlled epidural analgesia (PCEA) were given to the primiparae and the ones for mid-term delivery, and the intravenous anesthesia was given to those for induced abortion. Results Among the primiparae and the ones for mid-term delivery, VAS scores were rapidly reduced from 87.7?12.5 and 51.1?14.1 to 8.7?8.8 and 10.6?6.2, respectively, within 3 minutes by CLEA, the effective rates of analgesia were 94% and 100%, respectively. For all of them the Bromage evaluation was in 0 degree. After the induction of labor, no algesia memory existed in the women for induced abortion, the operative time was 15.28?2.57 minutes, the dosage of propoful was 162.9?39.8 mg (about 3mg/kg), and the dosage of fentanyl was 0.05 mg. The incidence of hypotension after intravenous anesthesia was 5.8%. Conclusions Intravenous anesthesia with propoful may remit the panic and fearful sense of pregnant women to induced abortion, and reduce the pain during operation. It is suggested to develop widely and carry out routinely the intravenous anesthesia as an effective and safe technique in gynecological analgesia.

Objective The objective of this paper is to statistically analyze what influence can the utilization of Traditional Chinese Medicine (TCM) have on healthcare expenses of inpatients, and to investigate whether TCM has an advantage of reducing healthcare expenses among certain diseases.Method: Inpatient medical records from 13 public general hospitals in Ningxia Hui autonomous region were collected to set up a database.A multiple linear regression models was established and used to analyze the impact of using TCM on hospitalization expenses.Results: The findings have shown that inpatients who apply TCM have significantly higher hospitalization expenses, with an estimated average of ?8 276.24, while those who do not apply TCM in their treatment spend ?7 254.59 on average (P=0.000).When the proportion of TCM expense higher than 10% of total drug expense, inpatients who apply the TCM have lower hospitalization expenses.In addition, hospitalization expenses decrease with the increase of TCM proportion.Among the diseases of the ear and mastoid disease, diseases of the circulatory system, and certain conditions originating in the perinatal period, inpatients who apply TCM have significant lower hospitalization expense (P=0.000).Hospitalization expense of inpatients who do not apply TCM is twice as much as that of those who apply TCM in the treatment of among diseases of the circulatory system, which has the largest TCM proportion.Conclusion: Only when applying TCM as main therapy can provide price advantage of TCM in the circulatory system caused by chronic diseases, and other advantages of treatment.