Objective To evaluate the effect of bile on growth and function of endothelial cells in vitro. Methods In vitro, endothelial cells explanted from human umbilical vein were cultured. The experiment was designed as 6 groups with concentration of 5%, 10%, 15%, 20%, 25% bile and without bile respectively. Morphological changes of the cells was evaluated daily until to harvest after culture. MTT value of light absorption and total protein was assessed at 5 days. von Willebrand factor (vWF) was measured for identification of cells and estimation of function. Results The explanted cells were verified as endothelial cells by vWF test. Viability and morphology of endothelial cells in culture mediums added with 5%, 10%, 15% bile were similar to those cells without bile, while cells in mediums with 20% and 25% bile were markedly reduced and immature. Comparing with the cells cultured without bile, MTT value of light absorption and total protein in mediums with 25% bile reduced statistical significantly ( P

Interventional techniques not only provide opportunity of treatment for many diseases,but also alter the traditional therapeutic pattern.With the new century of wide application of biological therapies, interventional technique also shows extensive roles.The current biological therapy,including gene therapy, cell transplantation therapy,immunobiologic molecule therapy containing cell factors,tumor antibody or vaccine,recombined proteins,radioactive-particles and targeting materials therapy,can be locally administrated by interventional techniques.The combination of targeting biological therapies and high-targeted interventional technique holds advantages of minimal invasion,accurate delivery,vigorous local effect,and less systemic adverse reactions.Authors believe that the biological therapy may arise a great opportunity for interventional radiology,therefore interventional colleagues should grasp firmly and promptly for the development and extension in this field.(J Intervent Radiol,2007,16:73-74)

BACKGROUND:We have built the three-dimensional human skin melanoma model with human epidermal keratinocytes and MV3 melanoma cel s co-cultured on the de-epidermized dermis in vitro.
OBJECTIVE:To establish a skin model of melanoma by mixed culture of MV3 melanoma cel s and HaCaT cel s on the de-epidermized dermis in vitro.
METHODS:MV3 melanoma cel s and HaCaT cel s were mixed with different percentages and inoculated on the surface of de-epidermized dermis fol owed by a liquid culture and air-liquid culture, and then the tissue-engineered skin model was established in vitro. Routine biopsy immunohistochemical observation was performed on the constructed skin melanoma model.
RESULTS AND CONCLUSION:Hematoxylin-eosin staining showed that MV3 melanoma cel s were distributed on the surface layer of de-epidermized dermis and formed tumor masses, while the HaCaT cel s were mixed growth with tumor cel s and formed a typical epidermoid structure. Some tumor cel s infiltrated into the surface or deep of de-epidermized dermis and showed a tumor foci distribution. The CK10, CK-pan and S-100 proteins were positive for immunohistochemical staining. With the increasing of MV3:HaCaT cel percentage, CK10 and CK-pan gradual y down-moved from the surface, and changed from layer distribution to lumpy distribution, while the staining of S-100 protein was gradual y distributed layer-by-layer, and some area showed tumor-like distribution. The results show that the skin model of melanoma can be in vitro constructed successful y by mixed culture of MV3 melanoma cel s and HaCaT cel s on de-epidermized dermis.

ObjectiveTo investigate the clinical appli cation of ultrasound in Peyronie disease. MethodsSixty-nine patients with Peyronie disease were examined by conventional ultrasound, and compared with pathology. ResultsUltrasonographic imaging showed the size and morphological of Peyronie plague,and located the position of the plagues.The ultrasound figures of plagues directly related to pathological components and case history,and they were roughly divided into twe types:hypoechoic type and hyperechoic type.ConclusionsUltrasound examination is the best and reliable method in choice for Peyronie plagues and evaluation of the therapeutic effects.

Animals ; Antigens, Nuclear ; metabolism ; Basic Helix-Loop-Helix Transcription Factors ; metabolism ; Biomarkers, Tumor ; metabolism ; Central Nervous System Neoplasms ; metabolism ; pathology ; Diagnosis, Differential ; Ependymoma ; metabolism ; pathology ; Humans ; Immunohistochemistry ; methods ; Intermediate Filament Proteins ; metabolism ; Medulloblastoma ; metabolism ; pathology ; Mucin-1 ; metabolism ; Nerve Tissue Proteins ; metabolism ; Nestin ; Neurocytoma ; metabolism ; pathology ; Oligodendrocyte Transcription Factor 2 ; Oligodendroglioma ; metabolism ; pathology

Interventional medicine plays an important role in the diagnosis and treatment of cardiovascular and cerebrovascular diseases, hepatobiliary tumors and other diseases, which has become the third largest type of treatment technology besides internal and surgical treatment. In recent years, with technological breakthroughs in imaging technology, robotic surgical system, artificial intelligence, Internet of Things and other fields, unprecedented opportunities have been provided for interventional/minimally invasive+robotics. Interventional robotic surgical systems have mushroomed around this field. The authors discuss the current status and future of interventional robotic surgical system with high recognition worldwide, especially the three categories of specialized robotic surgical system for vascular intervention, percutaneous puncture intervention and natural non-vascular luminal intervention.

BACKGROUND:Total knee arthroplasty had been generaly accepted as the final treatment plan, relieving pain and reconstructing function of knee joint. However, whether drainage tube can be used after replacement is stil controversial.
OBJECTIVE:To compare the clinical effects of drainageversusnondrainage after primary unilateral total knee arthroplasty.
METHODS:Total 102 patients undergoing primary unilateral total knee arthroplasty were randomly divided into 2 groups. In the drainagegroiup, a drainage tube was used. In the nondrainage group,
drainage tube was not used. Total blood loss was calculated by recording the hemoglobin and hematocrit before operation and that after 1, 3, 7 days of operation. The pain visual analogue scale scores, arthrocele, ecchymosis, infection rate, and deep venous thrombosis of lower extremity were examined and analyzed postoperatively. Knee Society Scores were recorded at 1 year postoperatively. Above indexes were compared between the two groups.
RESULTS AND CONCLUSION:(1) Total blood loss and blood transfusion rate were significantly higher in the drainage group than in the nondrainage group (P< 0.05). (2) There was no statisticaly significant difference in the pain visual analogue scale scores, arthrocele, ecchymosis, infection rate, and deep venous thrombosis of lower extremity between the two groups after replacement (P> 0.05). (3) No significant difference in Knee Society Scores was detected between the two groups (P> 0.05). (4) Results indicated that the total blood loss and blood transfusion rate may decrease significantly in patients without wound drainage after total knee arthroplasty. Limb sweling and ecchymosis area were not increased. No significant difference in infection, deep venousthrombosis of lower extremity and knee function was detectable between the two groups. Thus, total knee arthroplasty without wound drainage is safe and does not have obvious adverse consequences.

Mesoporous silica nanoparticles as drug carrier have become the new hot point in the field of biomedical application in recent years. This review focuses on the more recent developments and achievements on experimental design aspect of mesoporous silica nanoparticles with cancer diagnosis and therapy. The key advances of functionalization strategies of mesoporous silica nanoparticles with controlled release, tumor targeting and overcoming multidrug resistance are discussed in particular. Mesoporous silica nanoparticles as unique delivery systems have the potential to provide significantly a sound platform for cancer theranostic application.

Objective:To investigate the suppression effect and apoptosis of the colon cell line HT-29 treated with adenovirus-expressed human hyperplasia suppressor gene(hHSG)and hyperthermia.Methods:Replication-defective recombinant adenovirus containing hHSG was constructed,and then infected into HT-29 cell line to detect its overexpression by western blot.The synergic effect with hyperthermia was observed,cell survival was assessed using Vi-CELL TMXR Cell Viability Analyzer,and cell apoptosis and cell cycle distribution were determined by flow cytometry.Results:After the cells were treated with 100 MOI(multiplicity of infection)Ad5-hHSG and heated at 42 ℃ for 1 h,the number of viable cells decreased sharply,compared with single treatment with Ad5-hHSG or 42 ℃ for 1 h(P

Objective To study the characteristics of articles, citations and authors in Chinese Journal of Neurology(Chin J Neurol). Methods The date about articles, citations and authors of Chin J Neurol from 2002 to 2004 were collected and analyzed in terms of bibliomertic parameters. Results There were 648 papers published in Chin J Neurol during 3 years and papers with funded projects accounted for 25.0%. The average number of articles per isseu was 36; the average number of article per page was 0.43; the average publishing cycle of the articles was 10 months. The authors distributed in 27 mainland provinces and Hongkong, 91.5% of whom shared with co-author; the coporation rate was 4.19. The papers with citation accounted for 65.3% and 95.0% of all the citations came from journals. The Price’s indicator was 56.6%. Conclusions Chinese Journal of Neurology, an academic journal of high quality, with strong influence, pushes the subject of neurology in China forward.